Streptomyces sp. CB02488

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7296 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q5MJM2|A0A1Q5MJM2_9ACTN RNA methyltransferase OS=Streptomyces sp. CB02488 OX=1703920 GN=AMK09_27545 PE=3 SV=1
MM1 pKa = 8.11DD2 pKa = 4.66ISIDD6 pKa = 3.67FDD8 pKa = 3.79KK9 pKa = 11.39CCGAGQCVLAAPEE22 pKa = 4.3VFDD25 pKa = 5.26QRR27 pKa = 11.84DD28 pKa = 3.29EE29 pKa = 4.88DD30 pKa = 4.39GVVVLLDD37 pKa = 4.29DD38 pKa = 4.79NPPAGQTEE46 pKa = 4.34NVVQAAAACPAAVIQVRR63 pKa = 11.84VV64 pKa = 3.35

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q5LQP3|A0A1Q5LQP3_9ACTN Fatty acid--CoA ligase OS=Streptomyces sp. CB02488 OX=1703920 GN=AMK09_38170 PE=4 SV=1
MM1 pKa = 7.32TGAASAVVVTTGQAASVGTTTAGRR25 pKa = 11.84TGVTTAGPPTAAAASAATTAGPAAVAATSVATTTVVTGIVRR66 pKa = 11.84LGVTTAAVVPAVAVSGVTTAVVVRR90 pKa = 11.84PVVASAGTTAVAVPLVAGSVAMTAVVVLLVVGSVAMIGLRR130 pKa = 11.84VGMMTVVGVRR140 pKa = 11.84AVGSSVVTTAPVAAAAPAAASVGTTIVAVPAVVAPGATTAVVAPPVVGSVGTTAGPAVVAATSAATTIVVVPLVVGSVAMTAVVVRR226 pKa = 11.84PVVGSVVMIGLRR238 pKa = 11.84VGMMTVVGVRR248 pKa = 11.84AVGSSVVTTAPVGRR262 pKa = 11.84VATTTTVAVPLVVGSVGTTAVVVRR286 pKa = 11.84PVVGSVVMIGLRR298 pKa = 11.84VGMMTVVGVRR308 pKa = 11.84AVGSSVVTTAPVGRR322 pKa = 11.84VATTTTVAVPLAAASVGTTVVVVPAVVGSVGTTVVVVPAVVGSVGTTVVVVPAVVASVGTTAGPAVVAATSAATTTVVTGIVRR405 pKa = 11.84LGVTTAGEE413 pKa = 4.05ALPAVSVVGAMRR425 pKa = 11.84ATGVATAAATTVVGTSGGTSVSASRR450 pKa = 11.84SSGFPSLTRR459 pKa = 11.84SPATRR464 pKa = 11.84STRR467 pKa = 11.84MSGRR471 pKa = 11.84SS472 pKa = 3.17

Molecular weight: 43.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7295

1

7296

2468403

28

8759

338.3

36.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.55 ± 0.042

0.764 ± 0.007

6.017 ± 0.025

5.607 ± 0.027

2.765 ± 0.015

9.636 ± 0.029

2.255 ± 0.015

3.298 ± 0.02

2.215 ± 0.028

10.276 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.787 ± 0.012

1.832 ± 0.016

6.039 ± 0.025

2.786 ± 0.019

7.788 ± 0.03

5.309 ± 0.019

6.163 ± 0.022

8.321 ± 0.028

1.504 ± 0.012

2.088 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski