Wenzhou picorna-like virus 33

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KMD5|A0A1L3KMD5_9VIRU Uncharacterized protein OS=Wenzhou picorna-like virus 33 OX=1923619 PE=4 SV=1
MM1 pKa = 7.62SFTTNEE7 pKa = 3.99SEE9 pKa = 4.36VVVNVKK15 pKa = 9.99LLRR18 pKa = 11.84RR19 pKa = 11.84AEE21 pKa = 3.91RR22 pKa = 11.84EE23 pKa = 4.09AIQRR27 pKa = 11.84ACNIRR32 pKa = 11.84RR33 pKa = 11.84LKK35 pKa = 10.64KK36 pKa = 10.4AFKK39 pKa = 10.38SLPKK43 pKa = 10.1YY44 pKa = 9.98EE45 pKa = 4.18RR46 pKa = 11.84EE47 pKa = 3.89QVIASARR54 pKa = 11.84DD55 pKa = 3.27AKK57 pKa = 10.95YY58 pKa = 10.34SFVRR62 pKa = 11.84EE63 pKa = 3.82EE64 pKa = 4.24SGITDD69 pKa = 3.85YY70 pKa = 11.4LYY72 pKa = 11.31GAAAVAAGAAGAAAGFSIAKK92 pKa = 8.23TSHH95 pKa = 6.63EE96 pKa = 3.89IGEE99 pKa = 4.37AVKK102 pKa = 9.7DD103 pKa = 3.71TLSGVNAVMQPAGAIANSVGGALEE127 pKa = 3.93YY128 pKa = 10.56LYY130 pKa = 11.34SLVKK134 pKa = 10.11QVYY137 pKa = 7.49DD138 pKa = 3.42TCANIVGKK146 pKa = 10.05FSQLIFGIFVVAMKK160 pKa = 10.7ACFGLVNMASRR171 pKa = 11.84LFDD174 pKa = 3.53SVVSMVAPDD183 pKa = 3.58FHH185 pKa = 8.52LLDD188 pKa = 4.14SLEE191 pKa = 4.4EE192 pKa = 4.87DD193 pKa = 4.12DD194 pKa = 5.93VLPQSGCMSYY204 pKa = 10.8LPQVVTLVCTFFVPTYY220 pKa = 7.69STKK223 pKa = 10.69AYY225 pKa = 8.43MSEE228 pKa = 3.62VMRR231 pKa = 11.84RR232 pKa = 11.84VGSYY236 pKa = 10.93DD237 pKa = 3.0RR238 pKa = 11.84TASGISSVFTSIAMMVQDD256 pKa = 5.48CINVVLRR263 pKa = 11.84RR264 pKa = 11.84FGVDD268 pKa = 3.71EE269 pKa = 4.0IQLIGEE275 pKa = 4.22AEE277 pKa = 4.1KK278 pKa = 10.65QVLEE282 pKa = 4.14WCRR285 pKa = 11.84QVDD288 pKa = 4.11DD289 pKa = 3.92VFKK292 pKa = 10.73IIDD295 pKa = 3.65TSTPQIKK302 pKa = 10.15DD303 pKa = 3.35LQFAQSLLAVGYY315 pKa = 9.09NFKK318 pKa = 10.75KK319 pKa = 9.53VTTASHH325 pKa = 7.13LLHH328 pKa = 6.68TIDD331 pKa = 4.33RR332 pKa = 11.84TLEE335 pKa = 4.02KK336 pKa = 10.71LNHH339 pKa = 6.55KK340 pKa = 9.9LVAHH344 pKa = 6.62KK345 pKa = 10.98GIMNDD350 pKa = 3.09EE351 pKa = 4.07NAFRR355 pKa = 11.84QQPILVMFGGGSGIGKK371 pKa = 8.32TNLIKK376 pKa = 10.84SLAGAILQLADD387 pKa = 3.28MCKK390 pKa = 9.9PEE392 pKa = 5.37HH393 pKa = 5.92VAQNLWQKK401 pKa = 11.43GDD403 pKa = 3.34TQYY406 pKa = 11.12WNGYY410 pKa = 8.05VGQLVYY416 pKa = 11.07IMDD419 pKa = 4.4DD420 pKa = 3.3VFQKK424 pKa = 11.04KK425 pKa = 7.96EE426 pKa = 3.85VAGGAEE432 pKa = 4.04NEE434 pKa = 4.15GFTIIRR440 pKa = 11.84AVSNWPFPLNFADD453 pKa = 3.99VEE455 pKa = 4.88SKK457 pKa = 10.88GRR459 pKa = 11.84FYY461 pKa = 10.92FSSKK465 pKa = 10.8LMIGTTNVDD474 pKa = 3.97DD475 pKa = 4.09IQRR478 pKa = 11.84AAGGVLQYY486 pKa = 10.63PEE488 pKa = 3.96AVVRR492 pKa = 11.84RR493 pKa = 11.84IEE495 pKa = 3.82HH496 pKa = 6.26GYY498 pKa = 9.05WLEE501 pKa = 4.27LADD504 pKa = 5.29DD505 pKa = 4.25YY506 pKa = 11.03KK507 pKa = 10.88TEE509 pKa = 4.45RR510 pKa = 11.84KK511 pKa = 7.5TLDD514 pKa = 3.13YY515 pKa = 11.35AKK517 pKa = 10.35LKK519 pKa = 10.19RR520 pKa = 11.84VHH522 pKa = 6.94DD523 pKa = 3.87EE524 pKa = 3.72RR525 pKa = 11.84LRR527 pKa = 11.84TLFADD532 pKa = 4.85GNQPTKK538 pKa = 10.47MEE540 pKa = 4.29VLRR543 pKa = 11.84SFPWEE548 pKa = 3.7AFEE551 pKa = 5.34ARR553 pKa = 11.84PHH555 pKa = 5.95NFASGAPGRR564 pKa = 11.84VGGFTSLLDD573 pKa = 3.35IAVEE577 pKa = 3.76IAEE580 pKa = 4.14EE581 pKa = 3.91LKK583 pKa = 10.91ARR585 pKa = 11.84SEE587 pKa = 4.02RR588 pKa = 11.84HH589 pKa = 4.6EE590 pKa = 4.57AEE592 pKa = 4.17CTMTLDD598 pKa = 2.96WLTAISRR605 pKa = 11.84ARR607 pKa = 11.84PEE609 pKa = 4.3AGFSSALIDD618 pKa = 3.5PTVGVSSQASHH629 pKa = 6.82SGEE632 pKa = 4.02PPQTGDD638 pKa = 3.57PPSSLRR644 pKa = 11.84GTMEE648 pKa = 3.69RR649 pKa = 11.84QIDD652 pKa = 3.86DD653 pKa = 3.64LVARR657 pKa = 11.84RR658 pKa = 11.84LRR660 pKa = 11.84ASTSTTPEE668 pKa = 3.22EE669 pKa = 4.07RR670 pKa = 11.84TAEE673 pKa = 4.07FAAGLDD679 pKa = 4.34GVAEE683 pKa = 4.07MCIGDD688 pKa = 3.98IKK690 pKa = 10.84RR691 pKa = 11.84EE692 pKa = 3.92QEE694 pKa = 3.72KK695 pKa = 10.44RR696 pKa = 11.84RR697 pKa = 11.84TAIEE701 pKa = 4.05YY702 pKa = 9.81IVYY705 pKa = 10.13LFKK708 pKa = 10.57TVAPSVLALLNVAMFLASGAISVWLHH734 pKa = 4.68YY735 pKa = 10.8KK736 pKa = 10.27FIQGICSLVSYY747 pKa = 10.5AFKK750 pKa = 10.78CVLSLMEE757 pKa = 4.12MARR760 pKa = 11.84DD761 pKa = 4.72FIFGTTPVEE770 pKa = 3.91QSVHH774 pKa = 5.69VEE776 pKa = 4.0HH777 pKa = 6.68KK778 pKa = 9.39TMPRR782 pKa = 11.84KK783 pKa = 8.72KK784 pKa = 8.24TQLAPPSAVIQMGNPPADD802 pKa = 3.49NLADD806 pKa = 3.22IAYY809 pKa = 8.55KK810 pKa = 10.53NSYY813 pKa = 10.47KK814 pKa = 10.53IMLGNGEE821 pKa = 4.08EE822 pKa = 4.17AKK824 pKa = 10.75SVGQVLMLRR833 pKa = 11.84QSFGVMPCHH842 pKa = 7.21FLTCPEE848 pKa = 4.24GPLRR852 pKa = 11.84FISCAGNGHH861 pKa = 6.43VIHH864 pKa = 7.09SSVQALKK871 pKa = 9.71ACRR874 pKa = 11.84VIALPTSDD882 pKa = 3.86MVFVDD887 pKa = 4.92LKK889 pKa = 10.33PILVRR894 pKa = 11.84AHH896 pKa = 6.64RR897 pKa = 11.84DD898 pKa = 3.18IVKK901 pKa = 10.34HH902 pKa = 5.53FVTDD906 pKa = 3.75KK907 pKa = 10.08TWNTLKK913 pKa = 10.86DD914 pKa = 3.42MRR916 pKa = 11.84NTSVRR921 pKa = 11.84LDD923 pKa = 3.52VARR926 pKa = 11.84MSGDD930 pKa = 3.26SLDD933 pKa = 3.5RR934 pKa = 11.84HH935 pKa = 5.88VMYY938 pKa = 10.83SSFLRR943 pKa = 11.84FDD945 pKa = 3.6PQITTIVTQVDD956 pKa = 3.84NVWAYY961 pKa = 10.43DD962 pKa = 3.79CPTKK966 pKa = 10.9VGDD969 pKa = 4.05CGAVLSISEE978 pKa = 3.94PCYY981 pKa = 10.58YY982 pKa = 10.27GGKK985 pKa = 10.04AILGIHH991 pKa = 6.54IAGKK995 pKa = 8.22TNSLMSVGSRR1005 pKa = 11.84EE1006 pKa = 4.17GYY1008 pKa = 9.93AAALTLEE1015 pKa = 5.02FIEE1018 pKa = 5.05KK1019 pKa = 10.54ALDD1022 pKa = 3.44KK1023 pKa = 10.76FKK1025 pKa = 9.57PTPVVDD1031 pKa = 4.38RR1032 pKa = 11.84FHH1034 pKa = 7.42EE1035 pKa = 4.51DD1036 pKa = 3.07LEE1038 pKa = 4.59GRR1040 pKa = 11.84GIEE1043 pKa = 4.08LEE1045 pKa = 4.06EE1046 pKa = 4.36VEE1048 pKa = 6.07DD1049 pKa = 5.3IEE1051 pKa = 4.68TQCGVVGGSITYY1063 pKa = 10.13VGSLSAAHH1071 pKa = 6.79SISQAGKK1078 pKa = 8.77SKK1080 pKa = 10.64LMRR1083 pKa = 11.84TGFSGWGRR1091 pKa = 11.84CPVAPAPLRR1100 pKa = 11.84PVYY1103 pKa = 10.38RR1104 pKa = 11.84DD1105 pKa = 3.72GILIKK1110 pKa = 10.53PMHH1113 pKa = 6.07QAMANYY1119 pKa = 8.05KK1120 pKa = 10.56KK1121 pKa = 9.88PVQPSAVRR1129 pKa = 11.84NGAAIMGLAMKK1140 pKa = 8.42MHH1142 pKa = 6.71NLHH1145 pKa = 6.39TKK1147 pKa = 8.97HH1148 pKa = 5.89CTRR1151 pKa = 11.84KK1152 pKa = 9.32ILTFEE1157 pKa = 4.14EE1158 pKa = 4.67SVLGVPEE1165 pKa = 4.06MKK1167 pKa = 10.16IKK1169 pKa = 9.18NTNRR1173 pKa = 11.84STSPGWPWRR1182 pKa = 11.84LEE1184 pKa = 4.11CANGKK1189 pKa = 9.64RR1190 pKa = 11.84DD1191 pKa = 3.38IFGSGVEE1198 pKa = 3.95FDD1200 pKa = 5.12LYY1202 pKa = 10.1TEE1204 pKa = 3.99KK1205 pKa = 11.18AMALRR1210 pKa = 11.84GRR1212 pKa = 11.84VEE1214 pKa = 4.27EE1215 pKa = 4.07VLEE1218 pKa = 3.95AAKK1221 pKa = 10.44RR1222 pKa = 11.84GEE1224 pKa = 4.06RR1225 pKa = 11.84LAHH1228 pKa = 5.76VFADD1232 pKa = 4.09FLKK1235 pKa = 11.09DD1236 pKa = 3.15EE1237 pKa = 4.61TRR1239 pKa = 11.84PHH1241 pKa = 6.36AKK1243 pKa = 9.83VEE1245 pKa = 4.15QVMTRR1250 pKa = 11.84AISGAPLDD1258 pKa = 3.68YY1259 pKa = 10.52TIAVRR1264 pKa = 11.84MYY1266 pKa = 9.38FGAFLSSMFLHH1277 pKa = 5.79NTVSGMAPGLNYY1289 pKa = 10.64YY1290 pKa = 10.51SDD1292 pKa = 3.41WSTLARR1298 pKa = 11.84EE1299 pKa = 4.47LLRR1302 pKa = 11.84KK1303 pKa = 8.78GEE1305 pKa = 4.11KK1306 pKa = 9.41MFAGDD1311 pKa = 4.74FKK1313 pKa = 11.52AFDD1316 pKa = 3.88ASEE1319 pKa = 4.07QPDD1322 pKa = 3.4IHH1324 pKa = 8.36LLILDD1329 pKa = 5.11YY1330 pKa = 11.36INDD1333 pKa = 3.56WYY1335 pKa = 10.47DD1336 pKa = 3.15QYY1338 pKa = 11.92GADD1341 pKa = 3.53PEE1343 pKa = 4.39GRR1345 pKa = 11.84RR1346 pKa = 11.84VRR1348 pKa = 11.84TVLFEE1353 pKa = 4.45DD1354 pKa = 5.61LIHH1357 pKa = 6.46SRR1359 pKa = 11.84HH1360 pKa = 5.39LTGDD1364 pKa = 3.51SYY1366 pKa = 12.01KK1367 pKa = 10.68LDD1369 pKa = 5.11TIVQWNKK1376 pKa = 10.34SLPSGHH1382 pKa = 7.46PLTTAVNSMYY1392 pKa = 11.18SLFTLTACYY1401 pKa = 10.47VHH1403 pKa = 6.42LTKK1406 pKa = 10.81DD1407 pKa = 3.55HH1408 pKa = 6.38EE1409 pKa = 4.53NMWDD1413 pKa = 3.37HH1414 pKa = 7.04VFICTFGDD1422 pKa = 3.91DD1423 pKa = 3.99NVVSADD1429 pKa = 3.99DD1430 pKa = 3.5DD1431 pKa = 4.41TIAVFNQVSVAKK1443 pKa = 10.59AMQEE1447 pKa = 4.17LFHH1450 pKa = 6.4LTYY1453 pKa = 10.73TSDD1456 pKa = 3.42KK1457 pKa = 10.59KK1458 pKa = 10.89DD1459 pKa = 3.47AEE1461 pKa = 4.28LVPYY1465 pKa = 7.85EE1466 pKa = 4.53TIHH1469 pKa = 7.82DD1470 pKa = 3.58ITFLKK1475 pKa = 10.48RR1476 pKa = 11.84SFVRR1480 pKa = 11.84SQSDD1484 pKa = 3.68GGWIAPLAMDD1494 pKa = 4.74SILYY1498 pKa = 8.06RR1499 pKa = 11.84TYY1501 pKa = 10.63FYY1503 pKa = 10.95QNPRR1507 pKa = 11.84NYY1509 pKa = 9.97VRR1511 pKa = 11.84DD1512 pKa = 3.1QATNFKK1518 pKa = 9.18EE1519 pKa = 4.05ALMEE1523 pKa = 4.3LSLHH1527 pKa = 6.87GEE1529 pKa = 4.31EE1530 pKa = 4.69EE1531 pKa = 3.75WDD1533 pKa = 3.74EE1534 pKa = 4.3RR1535 pKa = 11.84FQAAAVYY1542 pKa = 10.54CRR1544 pKa = 11.84DD1545 pKa = 3.56NNIDD1549 pKa = 3.41FCITSRR1555 pKa = 11.84EE1556 pKa = 4.24QARR1559 pKa = 11.84QMCLARR1565 pKa = 11.84TDD1567 pKa = 3.03VWFF1570 pKa = 4.46

Molecular weight:
174.98 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KMD5|A0A1L3KMD5_9VIRU Uncharacterized protein OS=Wenzhou picorna-like virus 33 OX=1923619 PE=4 SV=1
MM1 pKa = 7.59LSGVLATASRR11 pKa = 11.84VPKK14 pKa = 10.42AVGRR18 pKa = 11.84LFPSLAPIMGSTSWFLAASARR39 pKa = 11.84AANAFGFSKK48 pKa = 10.52PVVQKK53 pKa = 10.26VPPVLRR59 pKa = 11.84TFHH62 pKa = 6.76NYY64 pKa = 8.98EE65 pKa = 4.3SNIDD69 pKa = 3.64MPVPAVAVGAFAGNSVAVSKK89 pKa = 11.11ALGGTDD95 pKa = 3.18IDD97 pKa = 4.44EE98 pKa = 4.31MSFDD102 pKa = 4.59YY103 pKa = 10.76ILSKK107 pKa = 10.35PSQIFRR113 pKa = 11.84GTMSTTDD120 pKa = 2.79AHH122 pKa = 6.0ATVEE126 pKa = 4.47YY127 pKa = 9.86ASHH130 pKa = 6.3VALTHH135 pKa = 4.44MWFRR139 pKa = 11.84APTTGVDD146 pKa = 3.06RR147 pKa = 11.84GNISFPRR154 pKa = 11.84GAPAVGGTTAVIPSNLLYY172 pKa = 10.22FAQHH176 pKa = 5.09FRR178 pKa = 11.84LWHH181 pKa = 6.1GDD183 pKa = 2.95LVYY186 pKa = 10.58RR187 pKa = 11.84VTFAKK192 pKa = 10.43SKK194 pKa = 8.65FHH196 pKa = 6.64TGRR199 pKa = 11.84VMFSFIPNYY208 pKa = 10.04QRR210 pKa = 11.84VTNVSTYY217 pKa = 10.95SEE219 pKa = 4.46AGLAGGPAPGVFAGDD234 pKa = 4.27LQPSQYY240 pKa = 11.5SLVFDD245 pKa = 5.06LKK247 pKa = 11.0DD248 pKa = 3.37GSEE251 pKa = 4.56FEE253 pKa = 4.92FEE255 pKa = 4.39VPYY258 pKa = 9.94IAPMPHH264 pKa = 6.95VGINDD269 pKa = 3.96SIGFVTMQIMDD280 pKa = 4.4PLVANGEE287 pKa = 4.24SSDD290 pKa = 3.78TISFIVEE297 pKa = 3.66VAAKK301 pKa = 9.82PGFYY305 pKa = 10.1FAGIAGPNVPIWPDD319 pKa = 3.2LTAPTIEE326 pKa = 4.29FQSGVGGITVDD337 pKa = 3.82ASQHH341 pKa = 5.06SVGEE345 pKa = 4.21KK346 pKa = 10.4FMSAKK351 pKa = 10.11QLMLHH356 pKa = 6.84PIMRR360 pKa = 11.84RR361 pKa = 11.84FSHH364 pKa = 6.77ANNTTAQGTIPLWPCSPAWGDD385 pKa = 3.74GAPLPVNTQRR395 pKa = 11.84QYY397 pKa = 10.41PFIRR401 pKa = 11.84SGMVAQCFAFGIGSTLLHH419 pKa = 6.2MQTAGLSVSSIVRR432 pKa = 11.84VVQSPGDD439 pKa = 3.44NGEE442 pKa = 4.24AVTGTVPGLYY452 pKa = 9.44NRR454 pKa = 11.84PIASPNTAWAQSSRR468 pKa = 11.84GPTSTEE474 pKa = 3.92SYY476 pKa = 10.1LLPMLSAAPRR486 pKa = 11.84FRR488 pKa = 11.84IGDD491 pKa = 3.79FNNASPTRR499 pKa = 11.84DD500 pKa = 3.16WSPGGSSTISTDD512 pKa = 2.98SLAVKK517 pKa = 7.42TTYY520 pKa = 9.89NWSVANVDD528 pKa = 3.07GATRR532 pKa = 11.84LWSWGVSAADD542 pKa = 4.62DD543 pKa = 3.92ARR545 pKa = 11.84CAAWIGPCPMVLANNGSTTNTFYY568 pKa = 10.67IDD570 pKa = 3.38GGVNNN575 pKa = 4.77

Molecular weight:
61.21 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

2145

575

1570

1072.5

118.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.604 ± 0.634

1.492 ± 0.428

5.361 ± 0.638

4.988 ± 1.466

4.942 ± 0.429

7.319 ± 1.02

2.471 ± 0.206

5.175 ± 0.164

4.382 ± 1.234

7.646 ± 1.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.17 ± 0.115

3.543 ± 0.526

4.848 ± 1.507

3.31 ± 0.19

5.315 ± 0.613

7.646 ± 1.126

6.107 ± 0.644

8.065 ± 0.152

1.492 ± 0.32

3.124 ± 0.277

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski