Hubei picorna-like virus 76
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KJM2|A0A1L3KJM2_9VIRU Calici_coat domain-containing protein OS=Hubei picorna-like virus 76 OX=1923160 PE=4 SV=1
MM1 pKa = 7.2 ATLAANQPDD10 pKa = 4.37 PGSGEE15 pKa = 4.13 IFDD18 pKa = 3.88 STAIRR23 pKa = 11.84 NEE25 pKa = 4.21 TPLSAVGGQGGPTQEE40 pKa = 4.45 APQHH44 pKa = 5.5 VGEE47 pKa = 4.89 VNTIDD52 pKa = 3.22 QFLRR56 pKa = 11.84 VQSIHH61 pKa = 7.05 LSTTTWSTSADD72 pKa = 3.22 AGTLLFSVPIHH83 pKa = 6.37 PSFTHH88 pKa = 6.46 VYY90 pKa = 10.13 LGHH93 pKa = 6.67 LWKK96 pKa = 9.99 MYY98 pKa = 9.72 NAWAGGTDD106 pKa = 3.67 FDD108 pKa = 5.92 LYY110 pKa = 10.9 INATGFHH117 pKa = 6.58 AGKK120 pKa = 9.97 LGQARR125 pKa = 11.84 IPPNIHH131 pKa = 6.84 PSTLRR136 pKa = 11.84 SISDD140 pKa = 3.09 WTAFEE145 pKa = 4.17 YY146 pKa = 10.61 QVLDD150 pKa = 3.74 AKK152 pKa = 10.02 TMQGSCSSIIDD163 pKa = 3.58 QRR165 pKa = 11.84 NTMYY169 pKa = 10.38 HH170 pKa = 5.27 YY171 pKa = 11.04 QDD173 pKa = 3.55 FDD175 pKa = 4.19 EE176 pKa = 5.08 NNPSTFGGYY185 pKa = 9.93 FCVFVIAPLATSSTGATSVSILIFSKK211 pKa = 9.98 PASNFTFAQLRR222 pKa = 11.84 PLKK225 pKa = 10.22 SAQNNAVIPPDD236 pKa = 3.65 LEE238 pKa = 3.94 KK239 pKa = 11.12 ALDD242 pKa = 3.74 FRR244 pKa = 11.84 RR245 pKa = 11.84 FAAEE249 pKa = 3.75 PVQGSEE255 pKa = 3.88 IQGMTVHH262 pKa = 6.29 SLKK265 pKa = 10.66 GKK267 pKa = 10.33 KK268 pKa = 8.87 ILKK271 pKa = 10.48 DD272 pKa = 3.58 EE273 pKa = 4.89 VINCAKK279 pKa = 10.5 FGGAAMYY286 pKa = 9.12 EE287 pKa = 4.27 HH288 pKa = 7.86 LFTPISSGAVRR299 pKa = 11.84 KK300 pKa = 9.34 KK301 pKa = 10.51 QNFVEE306 pKa = 4.43 LQANLQVVRR315 pKa = 11.84 VNGEE319 pKa = 3.56 NYY321 pKa = 8.48 IRR323 pKa = 11.84 SVGRR327 pKa = 11.84 SDD329 pKa = 5.31 LVFNTLTDD337 pKa = 3.6 NPKK340 pKa = 10.61 EE341 pKa = 3.93 PGIPYY346 pKa = 9.85 VDD348 pKa = 3.83 NLVCVLTFVAQKK360 pKa = 10.88 ADD362 pKa = 3.55 STVPSSVVAGFFTNNTKK379 pKa = 10.4 NPQFHH384 pKa = 6.46 GFWANFKK391 pKa = 10.91 DD392 pKa = 4.91 NAGNWDD398 pKa = 3.58 SYY400 pKa = 11.71 GFMDD404 pKa = 5.93 DD405 pKa = 4.49 EE406 pKa = 4.51 VPEE409 pKa = 4.61 LKK411 pKa = 9.91 TDD413 pKa = 3.55 TSYY416 pKa = 11.99 DD417 pKa = 3.56 LLLKK421 pKa = 10.89 NFFIQILQVTLPDD434 pKa = 3.46 SAKK437 pKa = 9.96 TYY439 pKa = 10.38 APPITEE445 pKa = 4.16 SVIDD449 pKa = 3.79 FQTYY453 pKa = 9.72 SRR455 pKa = 11.84 SSAQPLALQMLLKK468 pKa = 10.66 DD469 pKa = 3.76 PSFDD473 pKa = 3.52 SVLPDD478 pKa = 3.38 GQTLNFEE485 pKa = 4.63 LWDD488 pKa = 3.53 EE489 pKa = 4.35 HH490 pKa = 7.37 ANLPIMPLRR499 pKa = 11.84 LHH501 pKa = 7.06 RR502 pKa = 11.84 DD503 pKa = 3.33 GFFSTVAQDD512 pKa = 3.09 TDD514 pKa = 4.01 LVFDD518 pKa = 4.62 LTTHH522 pKa = 6.9 KK523 pKa = 10.83 YY524 pKa = 7.83 ALKK527 pKa = 10.66 YY528 pKa = 9.97 IGRR531 pKa = 11.84 SPATSALSAAVSRR544 pKa = 11.84 APSEE548 pKa = 4.02 YY549 pKa = 10.02 KK550 pKa = 10.52 RR551 pKa = 11.84 NFLHH555 pKa = 6.39 SRR557 pKa = 11.84 LVATVDD563 pKa = 3.79 GEE565 pKa = 4.69 LKK567 pKa = 10.64 FF568 pKa = 4.27
Molecular weight: 62.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.5
IPC2_protein 5.397
IPC_protein 5.436
Toseland 5.817
ProMoST 5.741
Dawson 5.639
Bjellqvist 5.677
Wikipedia 5.626
Rodwell 5.601
Grimsley 5.982
Solomon 5.639
Lehninger 5.626
Nozaki 5.881
DTASelect 6.084
Thurlkill 6.02
EMBOSS 5.995
Sillero 5.97
Patrickios 4.062
IPC_peptide 5.652
IPC2_peptide 5.982
IPC2.peptide.svr19 5.911
Protein with the highest isoelectric point:
>tr|A0A1L3KJC6|A0A1L3KJC6_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 76 OX=1923160 PE=4 SV=1
MM1 pKa = 6.33 SHH3 pKa = 6.45 SRR5 pKa = 11.84 ISHH8 pKa = 5.69 TGSMLAQDD16 pKa = 5.03 KK17 pKa = 10.34 KK18 pKa = 10.07 VCTNFTPDD26 pKa = 4.27 DD27 pKa = 3.66 FCAACLRR34 pKa = 11.84 FLNLRR39 pKa = 11.84 LEE41 pKa = 4.48 YY42 pKa = 10.77 DD43 pKa = 3.72 DD44 pKa = 5.85 DD45 pKa = 3.85 LHH47 pKa = 7.01 EE48 pKa = 4.97 FYY50 pKa = 10.91 EE51 pKa = 4.28 LQQEE55 pKa = 4.26 NKK57 pKa = 9.98 IFFCSLRR64 pKa = 11.84 TAQRR68 pKa = 11.84 CFGIIKK74 pKa = 9.7 RR75 pKa = 11.84 YY76 pKa = 9.99 KK77 pKa = 9.29 KK78 pKa = 9.39 IHH80 pKa = 5.93 VNFSIASPGTPDD92 pKa = 3.19 QVKK95 pKa = 9.18 EE96 pKa = 4.0 AVSRR100 pKa = 11.84 FQGLSTMQKK109 pKa = 9.37 MSVLNSNHH117 pKa = 5.96 IFFVPSSVLSQNFEE131 pKa = 3.99 PYY133 pKa = 9.79 IPEE136 pKa = 6.12 LITSMLPDD144 pKa = 3.68 TYY146 pKa = 11.22 AIINEE151 pKa = 3.98 NPFYY155 pKa = 10.8 NRR157 pKa = 11.84 NGMWEE162 pKa = 4.2 LCQPQIEE169 pKa = 4.63 FYY171 pKa = 10.67 EE172 pKa = 4.59 YY173 pKa = 8.43 ITGVFDD179 pKa = 4.22 CKK181 pKa = 10.64 YY182 pKa = 9.69 RR183 pKa = 11.84 HH184 pKa = 6.38 KK185 pKa = 11.1 NCDD188 pKa = 3.2 FPTPNFDD195 pKa = 3.83 RR196 pKa = 11.84 SSKK199 pKa = 10.24 PVGKK203 pKa = 9.88 EE204 pKa = 3.34 WILIDD209 pKa = 4.82 QPDD212 pKa = 4.05 SLHH215 pKa = 6.17 FASIADD221 pKa = 3.23 IRR223 pKa = 11.84 AYY225 pKa = 9.52 MHH227 pKa = 7.16 DD228 pKa = 3.59 VAKK231 pKa = 10.81 KK232 pKa = 9.23 EE233 pKa = 4.15 VGYY236 pKa = 10.49 HH237 pKa = 6.06 PKK239 pKa = 10.25 LDD241 pKa = 3.55 KK242 pKa = 11.12 KK243 pKa = 9.58 FTNHH247 pKa = 5.43 VYY249 pKa = 9.16 ATATRR254 pKa = 11.84 QYY256 pKa = 10.82 PVMRR260 pKa = 11.84 EE261 pKa = 3.73 KK262 pKa = 10.75 DD263 pKa = 3.63 VKK265 pKa = 8.19 TWKK268 pKa = 10.05 HH269 pKa = 4.37 ALLWYY274 pKa = 9.05 EE275 pKa = 4.08 HH276 pKa = 6.66 SRR278 pKa = 11.84 LCHH281 pKa = 5.35 EE282 pKa = 4.59 MLRR285 pKa = 11.84 GQALRR290 pKa = 11.84 LGLDD294 pKa = 3.57 CTPPCDD300 pKa = 3.77 WPPDD304 pKa = 3.87 PSEE307 pKa = 4.07 HH308 pKa = 6.43 LLYY311 pKa = 10.43 HH312 pKa = 6.47 IPDD315 pKa = 3.69 SEE317 pKa = 4.4 EE318 pKa = 3.55 IAEE321 pKa = 4.32 EE322 pKa = 4.67 FSQHH326 pKa = 4.67 EE327 pKa = 4.16 QKK329 pKa = 10.67 EE330 pKa = 4.32 EE331 pKa = 3.69 KK332 pKa = 10.38 KK333 pKa = 10.74 IIINCLRR340 pKa = 11.84 QKK342 pKa = 10.67 NHH344 pKa = 5.44 VDD346 pKa = 2.73 KK347 pKa = 10.86 HH348 pKa = 6.33 VYY350 pKa = 9.94 QIAQSTPVEE359 pKa = 4.04 KK360 pKa = 10.58 FEE362 pKa = 4.3 YY363 pKa = 10.47 SLTFEE368 pKa = 5.39 PYY370 pKa = 9.95 PIPPKK375 pKa = 9.12 RR376 pKa = 11.84 THH378 pKa = 5.57 IEE380 pKa = 3.55 KK381 pKa = 10.48 RR382 pKa = 11.84 EE383 pKa = 4.16 PIVMAEE389 pKa = 4.08 EE390 pKa = 4.65 VTCTRR395 pKa = 11.84 NSFPVIHH402 pKa = 6.69 AKK404 pKa = 10.02 KK405 pKa = 9.83 NKK407 pKa = 7.76 PAIVKK412 pKa = 10.0 FEE414 pKa = 4.59 SNPIFEE420 pKa = 4.31 YY421 pKa = 10.96 AKK423 pKa = 10.15 LNNMCTPRR431 pKa = 11.84 GSDD434 pKa = 3.14 FWEE437 pKa = 4.4 DD438 pKa = 3.1 RR439 pKa = 11.84 VAGAPHH445 pKa = 6.86 PLFYY449 pKa = 10.51 NYY451 pKa = 10.16 CIEE454 pKa = 4.5 RR455 pKa = 11.84 FGEE458 pKa = 4.47 KK459 pKa = 9.96 YY460 pKa = 10.78 GKK462 pKa = 10.15 DD463 pKa = 3.24 YY464 pKa = 11.31 SYY466 pKa = 11.77 FMWFKK471 pKa = 11.24 YY472 pKa = 9.61 GDD474 pKa = 3.26 QVFDD478 pKa = 3.97 HH479 pKa = 6.65 KK480 pKa = 11.14 VVHH483 pKa = 6.03 YY484 pKa = 10.25 KK485 pKa = 10.84 NSFHH489 pKa = 7.64 DD490 pKa = 4.42 FVNKK494 pKa = 9.46 VCKK497 pKa = 8.71 TVQMPKK503 pKa = 9.99 KK504 pKa = 9.03 KK505 pKa = 9.47 HH506 pKa = 5.06 LRR508 pKa = 11.84 RR509 pKa = 11.84 IAFKK513 pKa = 10.84 QPMSTIMSEE522 pKa = 3.98 DD523 pKa = 3.45 EE524 pKa = 4.07 NRR526 pKa = 11.84 FSALEE531 pKa = 4.03 EE532 pKa = 4.02 EE533 pKa = 4.78 LLDD536 pKa = 5.83 DD537 pKa = 3.99 GTNYY541 pKa = 9.88 QPEE544 pKa = 3.84 RR545 pKa = 11.84 RR546 pKa = 11.84 KK547 pKa = 10.16 RR548 pKa = 11.84 FKK550 pKa = 10.64 SKK552 pKa = 10.65 KK553 pKa = 9.19 AIKK556 pKa = 10.02 KK557 pKa = 9.85 KK558 pKa = 10.69 LEE560 pKa = 3.82 KK561 pKa = 9.76 LTQKK565 pKa = 10.46 RR566 pKa = 11.84 KK567 pKa = 9.57 ARR569 pKa = 11.84 KK570 pKa = 6.83 EE571 pKa = 3.76 QEE573 pKa = 4.21 RR574 pKa = 11.84 IQALYY579 pKa = 10.08 DD580 pKa = 3.43 APAYY584 pKa = 8.68 AQHH587 pKa = 6.43 VEE589 pKa = 4.07 PFYY592 pKa = 10.97 LVCQSKK598 pKa = 10.18 EE599 pKa = 3.84 VFYY602 pKa = 11.17 DD603 pKa = 3.72 ALEE606 pKa = 4.61 PDD608 pKa = 3.27 QKK610 pKa = 11.61 KK611 pKa = 10.14 NLLEE615 pKa = 4.2 KK616 pKa = 10.6 AADD619 pKa = 3.53 FTFQQKK625 pKa = 10.37 KK626 pKa = 10.1 KK627 pKa = 9.45 ISEE630 pKa = 4.43 KK631 pKa = 10.52 IEE633 pKa = 3.91 DD634 pKa = 4.74 FYY636 pKa = 11.87 DD637 pKa = 3.26 KK638 pKa = 11.15 TKK640 pKa = 10.66 FICANMTLDD649 pKa = 4.48 DD650 pKa = 4.32 YY651 pKa = 11.92 EE652 pKa = 5.53 HH653 pKa = 7.46 LLEE656 pKa = 5.36 KK657 pKa = 9.98 YY658 pKa = 10.47 KK659 pKa = 10.93 PMNTLNLIAQWWSCEE674 pKa = 4.26 SITEE678 pKa = 4.24 DD679 pKa = 3.73 AACVAAFLEE688 pKa = 4.3 LHH690 pKa = 5.41 EE691 pKa = 5.07 MYY693 pKa = 7.64 WTTEE697 pKa = 3.81 LTKK700 pKa = 10.64 LKK702 pKa = 11.0 VMLTPVIVFCLNVFKK717 pKa = 10.72 GWWKK721 pKa = 10.9 KK722 pKa = 10.47 KK723 pKa = 10.57 DD724 pKa = 3.16 QDD726 pKa = 3.95 LYY728 pKa = 10.83 EE729 pKa = 4.22 FQNFEE734 pKa = 3.86 QSPAHH739 pKa = 6.18 SFIRR743 pKa = 11.84 DD744 pKa = 3.5 TLGFDD749 pKa = 3.59 VPAEE753 pKa = 4.06 ILPTAAIVVCVGILLCGGIIPHH775 pKa = 6.4 MFRR778 pKa = 11.84 NFKK781 pKa = 9.94 WILKK785 pKa = 8.69 SLSSITAILKK795 pKa = 10.29 LSSCFEE801 pKa = 4.42 SGFSALFDD809 pKa = 3.88 GLSSVIGKK817 pKa = 9.37 LFGYY821 pKa = 8.46 TYY823 pKa = 10.32 IKK825 pKa = 10.61 EE826 pKa = 4.08 EE827 pKa = 4.04 EE828 pKa = 4.03 ADD830 pKa = 3.78 VISFKK835 pKa = 10.87 QSLCSFRR842 pKa = 11.84 EE843 pKa = 3.82 RR844 pKa = 11.84 LGEE847 pKa = 3.76 MSTALNHH854 pKa = 6.17 NPTSVMDD861 pKa = 4.37 DD862 pKa = 3.2 EE863 pKa = 5.4 EE864 pKa = 4.75 KK865 pKa = 10.98 FNEE868 pKa = 4.65 LEE870 pKa = 4.05 ALYY873 pKa = 10.63 QEE875 pKa = 4.69 LDD877 pKa = 3.37 GVYY880 pKa = 10.53 LRR882 pKa = 11.84 LISIKK887 pKa = 10.83 ANLSNCRR894 pKa = 11.84 PLFEE898 pKa = 6.82 DD899 pKa = 3.33 IRR901 pKa = 11.84 QEE903 pKa = 3.98 FKK905 pKa = 10.84 AIQTVVRR912 pKa = 11.84 SARR915 pKa = 11.84 QKK917 pKa = 7.59 TCRR920 pKa = 11.84 KK921 pKa = 9.45 IEE923 pKa = 4.03 PVWIFISGVPGVGKK937 pKa = 9.95 SQLISAIQRR946 pKa = 11.84 GISEE950 pKa = 3.92 KK951 pKa = 9.87 MGRR954 pKa = 11.84 KK955 pKa = 5.69 VTSYY959 pKa = 11.08 ARR961 pKa = 11.84 NSGEE965 pKa = 4.21 KK966 pKa = 9.25 HH967 pKa = 4.0 WSGYY971 pKa = 7.98 TGQTFVTYY979 pKa = 10.89 DD980 pKa = 3.86 DD981 pKa = 5.01 FSQSKK986 pKa = 10.43 VDD988 pKa = 3.68 TDD990 pKa = 3.77 HH991 pKa = 7.76 ADD993 pKa = 2.76 IMLFKK998 pKa = 10.8 NPNKK1002 pKa = 7.96 TTLIMSDD1009 pKa = 3.06 NSEE1012 pKa = 4.17 KK1013 pKa = 10.57 GRR1015 pKa = 11.84 QFSSPFVITASNEE1028 pKa = 3.75 IDD1030 pKa = 3.84 CKK1032 pKa = 11.04 NSASVKK1038 pKa = 10.74 NGVALNRR1045 pKa = 11.84 RR1046 pKa = 11.84 RR1047 pKa = 11.84 DD1048 pKa = 3.66 VLILVSDD1055 pKa = 4.42 PEE1057 pKa = 4.04 LAMFQNQHH1065 pKa = 3.99 GCYY1068 pKa = 8.35 PYY1070 pKa = 11.34 EE1071 pKa = 4.25 MLSDD1075 pKa = 4.13 DD1076 pKa = 4.6 PNLNGNPIITDD1087 pKa = 3.53 RR1088 pKa = 11.84 FGVTRR1093 pKa = 11.84 HH1094 pKa = 5.86 FYY1096 pKa = 10.98 DD1097 pKa = 3.64 PNFANLVINEE1107 pKa = 4.05 ISTDD1111 pKa = 3.35 STRR1114 pKa = 11.84 ADD1116 pKa = 3.4 YY1117 pKa = 10.4 RR1118 pKa = 11.84 GKK1120 pKa = 10.5 VINVQAVIDD1129 pKa = 4.19 KK1130 pKa = 10.46 AWRR1133 pKa = 11.84 AHH1135 pKa = 6.02 LKK1137 pKa = 10.32 YY1138 pKa = 8.93 KK1139 pKa = 10.15 QSYY1142 pKa = 7.95 IGSVEE1147 pKa = 4.09 VSSQLYY1153 pKa = 7.03 EE1154 pKa = 3.94 ACEE1157 pKa = 4.18 ASSSDD1162 pKa = 3.74 CDD1164 pKa = 3.61 VTPVMSEE1171 pKa = 3.48 ITRR1174 pKa = 11.84 SKK1176 pKa = 7.89 QQRR1179 pKa = 11.84 VRR1181 pKa = 11.84 LLPGEE1186 pKa = 4.02 VDD1188 pKa = 3.13 HH1189 pKa = 7.06 RR1190 pKa = 11.84 AKK1192 pKa = 10.75 KK1193 pKa = 10.36 KK1194 pKa = 10.25 FEE1196 pKa = 4.05 KK1197 pKa = 10.57 AQEE1200 pKa = 4.52 LINYY1204 pKa = 6.27 WTPTGDD1210 pKa = 4.33 YY1211 pKa = 11.19 ADD1213 pKa = 4.64 LEE1215 pKa = 4.66 QNFEE1219 pKa = 4.17 NFCADD1224 pKa = 3.14 MEE1226 pKa = 4.75 TYY1228 pKa = 10.13 QLQSGEE1234 pKa = 4.42 SFGSYY1239 pKa = 9.55 VYY1241 pKa = 11.04 GRR1243 pKa = 11.84 GKK1245 pKa = 10.54 VIAIGGPPGTGKK1257 pKa = 7.55 TTILDD1262 pKa = 3.48 MVKK1265 pKa = 10.54 DD1266 pKa = 3.99 HH1267 pKa = 7.15 FGEE1270 pKa = 4.36 LCTRR1274 pKa = 11.84 ISGVDD1279 pKa = 3.7 LLLQKK1284 pKa = 10.67 PEE1286 pKa = 4.07 TPVVIIEE1293 pKa = 4.88 DD1294 pKa = 3.61 GSTTKK1299 pKa = 10.64 EE1300 pKa = 3.48 IFNALKK1306 pKa = 10.2 QLVVTTYY1313 pKa = 11.33 DD1314 pKa = 3.34 SLSPIQVLFFSYY1326 pKa = 10.66 NKK1328 pKa = 9.81 EE1329 pKa = 3.73 LAKK1332 pKa = 10.43 SYY1334 pKa = 10.84 CHH1336 pKa = 6.97 DD1337 pKa = 3.98 EE1338 pKa = 4.04 EE1339 pKa = 4.04 WTLFYY1344 pKa = 10.88 RR1345 pKa = 11.84 RR1346 pKa = 11.84 LEE1348 pKa = 4.26 EE1349 pKa = 4.24 IEE1351 pKa = 4.12 TNFRR1355 pKa = 11.84 IKK1357 pKa = 10.84 GKK1359 pKa = 10.37 GIWSCIRR1366 pKa = 11.84 SHH1368 pKa = 7.54 LGVGKK1373 pKa = 10.37 KK1374 pKa = 9.9 NSYY1377 pKa = 11.17 EE1378 pKa = 3.88 NVQLNGTNINEE1389 pKa = 4.09 YY1390 pKa = 9.6 VGYY1393 pKa = 10.1 RR1394 pKa = 11.84 FKK1396 pKa = 11.22 GVNYY1400 pKa = 8.01 TQAAIVTLLTSVEE1413 pKa = 4.21 VKK1415 pKa = 10.66 EE1416 pKa = 4.73 EE1417 pKa = 3.97 EE1418 pKa = 4.18 FKK1420 pKa = 11.37 VAVCHH1425 pKa = 5.96 CCPVIPRR1432 pKa = 11.84 RR1433 pKa = 11.84 LVSATFEE1440 pKa = 4.03 VEE1442 pKa = 3.77 YY1443 pKa = 10.4 RR1444 pKa = 11.84 GPFSEE1449 pKa = 5.03 FSGGLPSAHH1458 pKa = 6.77 FDD1460 pKa = 3.54 LSKK1463 pKa = 11.21 VSLKK1467 pKa = 10.64 KK1468 pKa = 10.85 GSLTKK1473 pKa = 10.78 ALDD1476 pKa = 4.69 LIQNFLMPYY1485 pKa = 10.12 DD1486 pKa = 3.72 SEE1488 pKa = 4.37 NNKK1491 pKa = 9.45 FVTPDD1496 pKa = 4.31 TIPEE1500 pKa = 3.97 FCVFMNNMNKK1510 pKa = 9.12 VCTSEE1515 pKa = 4.92 LSAHH1519 pKa = 6.63 LKK1521 pKa = 10.59 FSDD1524 pKa = 3.43 GEE1526 pKa = 4.36 FYY1528 pKa = 11.34 VRR1530 pKa = 11.84 TRR1532 pKa = 11.84 AMAPYY1537 pKa = 9.36 VLRR1540 pKa = 11.84 FFKK1543 pKa = 10.91 VDD1545 pKa = 3.64 DD1546 pKa = 3.56 VDD1548 pKa = 5.78 FIDD1551 pKa = 4.99 GKK1553 pKa = 10.46 IVYY1556 pKa = 9.01 PDD1558 pKa = 3.46 KK1559 pKa = 10.85 TEE1561 pKa = 3.83 SWTKK1565 pKa = 7.83 TTASEE1570 pKa = 4.15 YY1571 pKa = 10.53 AQVFSKK1577 pKa = 10.82 EE1578 pKa = 3.55 QLQILNCKK1586 pKa = 9.9 FKK1588 pKa = 10.58 DD1589 pKa = 3.16 IKK1591 pKa = 11.11 AITDD1595 pKa = 3.53 TTASAIGMDD1604 pKa = 3.67 TFKK1607 pKa = 11.29 DD1608 pKa = 3.97 HH1609 pKa = 6.96 NPKK1612 pKa = 10.3 FYY1614 pKa = 10.88 GALRR1618 pKa = 11.84 IAAFVAKK1625 pKa = 9.83 IAVSAFSIYY1634 pKa = 10.55 KK1635 pKa = 9.49 LLPMHH1640 pKa = 6.21 GRR1642 pKa = 11.84 KK1643 pKa = 9.59 LFGAMEE1649 pKa = 4.95 SDD1651 pKa = 2.99 TRR1653 pKa = 11.84 DD1654 pKa = 2.88 RR1655 pKa = 11.84 TKK1657 pKa = 10.33 IKK1659 pKa = 10.48 KK1660 pKa = 9.28 SAKK1663 pKa = 9.37 QEE1665 pKa = 3.71 YY1666 pKa = 7.96 TQYY1669 pKa = 11.27 FDD1671 pKa = 5.59 LNTGEE1676 pKa = 4.49 YY1677 pKa = 9.71 EE1678 pKa = 4.12 WVSDD1682 pKa = 3.85 YY1683 pKa = 11.53 DD1684 pKa = 3.81 SEE1686 pKa = 4.92 SRR1688 pKa = 11.84 PEE1690 pKa = 4.14 PKK1692 pKa = 8.84 PTPQRR1697 pKa = 11.84 IVIDD1701 pKa = 3.85 PVLEE1705 pKa = 3.98 SRR1707 pKa = 11.84 PEE1709 pKa = 4.01 VKK1711 pKa = 8.69 PTPQRR1716 pKa = 11.84 VVVDD1720 pKa = 4.85 PILLPEE1726 pKa = 4.35 ASIDD1730 pKa = 3.61 QNSRR1734 pKa = 11.84 QIAKK1738 pKa = 9.83 IVTKK1742 pKa = 11.06 NMVQLGTIEE1751 pKa = 4.09 NGEE1754 pKa = 4.28 FEE1756 pKa = 4.57 HH1757 pKa = 6.92 KK1758 pKa = 9.21 VWAMMVIGRR1767 pKa = 11.84 CGATVGHH1774 pKa = 5.49 VVKK1777 pKa = 10.67 DD1778 pKa = 3.61 QEE1780 pKa = 5.17 LYY1782 pKa = 11.2 ALDD1785 pKa = 3.65 SRR1787 pKa = 11.84 FDD1789 pKa = 3.2 KK1790 pKa = 10.36 TQFYY1794 pKa = 9.57 PIKK1797 pKa = 9.32 ILKK1800 pKa = 10.02 RR1801 pKa = 11.84 SVSHH1805 pKa = 7.26 DD1806 pKa = 3.27 VTIFQVNDD1814 pKa = 2.81 VHH1816 pKa = 7.14 CPNFRR1821 pKa = 11.84 DD1822 pKa = 3.41 IAQYY1826 pKa = 10.8 LPSKK1830 pKa = 10.94 NEE1832 pKa = 3.7 LSQTSLYY1839 pKa = 10.73 SGSQGILWIKK1849 pKa = 10.56 SKK1851 pKa = 10.7 INEE1854 pKa = 4.57 SPTTLQPINVKK1865 pKa = 9.67 IDD1867 pKa = 3.23 AVVQKK1872 pKa = 10.34 RR1873 pKa = 11.84 CVSGDD1878 pKa = 3.62 DD1879 pKa = 3.48 VLTLNGIGYY1888 pKa = 9.09 HH1889 pKa = 6.01 GFMTAVKK1896 pKa = 10.35 VHH1898 pKa = 5.95 GADD1901 pKa = 3.92 LGTSPGDD1908 pKa = 3.55 CGSPLMLLNPLCRR1921 pKa = 11.84 FKK1923 pKa = 11.17 IMGLHH1928 pKa = 6.6 CAANANSGLSNYY1940 pKa = 9.11 LVRR1943 pKa = 11.84 EE1944 pKa = 4.28 LFIGMHH1950 pKa = 5.9 VEE1952 pKa = 4.09 MAGKK1956 pKa = 9.47 CQANLPKK1963 pKa = 10.47 VYY1965 pKa = 10.41 VLSHH1969 pKa = 5.63 QEE1971 pKa = 3.96 FTPVPPKK1978 pKa = 10.62 EE1979 pKa = 4.52 SLCGMPIVGDD1989 pKa = 3.99 LKK1991 pKa = 9.91 MQHH1994 pKa = 5.67 VPEE1997 pKa = 5.07 KK1998 pKa = 10.39 SRR2000 pKa = 11.84 LWKK2003 pKa = 10.77 SPLALPGKK2011 pKa = 9.94 NIYY2014 pKa = 10.06 EE2015 pKa = 4.09 PTILSPIDD2023 pKa = 3.8 DD2024 pKa = 3.76 RR2025 pKa = 11.84 NKK2027 pKa = 9.77 TDD2029 pKa = 3.12 RR2030 pKa = 11.84 HH2031 pKa = 5.78 PYY2033 pKa = 9.12 YY2034 pKa = 11.14 SNLAKK2039 pKa = 10.03 WSHH2042 pKa = 6.18 DD2043 pKa = 3.43 QPNLDD2048 pKa = 4.18 EE2049 pKa = 4.83 EE2050 pKa = 5.86 LIDD2053 pKa = 4.56 ACVEE2057 pKa = 4.12 DD2058 pKa = 3.81 LAEE2061 pKa = 4.83 RR2062 pKa = 11.84 YY2063 pKa = 9.23 AQEE2066 pKa = 4.1 IYY2068 pKa = 8.86 QTGFPVKK2075 pKa = 10.37 KK2076 pKa = 9.04 LTKK2079 pKa = 8.08 TQAVNSGKK2087 pKa = 10.37 EE2088 pKa = 3.89 YY2089 pKa = 10.56 GVNPIAMSSSPGYY2102 pKa = 9.78 PWTCGQASKK2111 pKa = 11.07 RR2112 pKa = 11.84 KK2113 pKa = 8.82 FFSLHH2118 pKa = 4.87 VSNDD2122 pKa = 3.32 QSHH2125 pKa = 5.95 MFYY2128 pKa = 10.73 SIADD2132 pKa = 3.57 TSEE2135 pKa = 3.41 GKK2137 pKa = 10.12 RR2138 pKa = 11.84 LHH2140 pKa = 6.04 NAIDD2144 pKa = 3.84 ALVTSCAQGKK2154 pKa = 9.34 VSAVVFAGHH2163 pKa = 7.44 LKK2165 pKa = 10.59 DD2166 pKa = 4.16 EE2167 pKa = 4.51 PVKK2170 pKa = 9.67 ATKK2173 pKa = 10.0 IQEE2176 pKa = 4.0 CTTRR2180 pKa = 11.84 SFAGAPLDD2188 pKa = 3.55 YY2189 pKa = 10.86 VVAHH2193 pKa = 6.36 RR2194 pKa = 11.84 QYY2196 pKa = 10.72 FAAALGAINSVRR2208 pKa = 11.84 HH2209 pKa = 5.43 TLPSQIGINPTSMEE2223 pKa = 3.38 WHH2225 pKa = 6.47 LLVSRR2230 pKa = 11.84 LLEE2233 pKa = 3.95 HH2234 pKa = 6.86 SPIGFDD2240 pKa = 4.82 LDD2242 pKa = 3.94 FKK2244 pKa = 11.35 NFDD2247 pKa = 3.58 STVPSLIMSKK2257 pKa = 9.78 IYY2259 pKa = 10.29 KK2260 pKa = 9.0 IYY2262 pKa = 10.9 NRR2264 pKa = 11.84 IFEE2267 pKa = 4.47 VNDD2270 pKa = 3.78 PNVTEE2275 pKa = 4.13 VEE2277 pKa = 4.13 QIIRR2281 pKa = 11.84 KK2282 pKa = 9.02 SLHH2285 pKa = 6.1 HH2286 pKa = 6.76 CLTHH2290 pKa = 6.87 PLLACVHH2297 pKa = 5.65 PTGTVALQAPGGQVSGQPATSTDD2320 pKa = 2.89 NCFVHH2325 pKa = 6.76 MIYY2328 pKa = 10.9 LMYY2331 pKa = 11.01 AWMTIFKK2338 pKa = 10.07 DD2339 pKa = 3.6 DD2340 pKa = 3.95 PLGNFSAFWKK2350 pKa = 9.36 NVKK2353 pKa = 9.97 PIVYY2357 pKa = 10.2 GDD2359 pKa = 3.87 DD2360 pKa = 3.0 GCYY2363 pKa = 10.2 AVDD2366 pKa = 3.79 PSVIDD2371 pKa = 3.56 RR2372 pKa = 11.84 FNLISVSQIMQNIGITCTGASKK2394 pKa = 10.43 QGLMQPFKK2402 pKa = 10.27 EE2403 pKa = 4.24 VKK2405 pKa = 9.49 NFEE2408 pKa = 4.26 FIKK2411 pKa = 10.1 RR2412 pKa = 11.84 TFVQAGPYY2420 pKa = 8.95 WIGPLHH2426 pKa = 5.93 PTSFFKK2432 pKa = 10.42 MLNYY2436 pKa = 10.51 SKK2438 pKa = 10.93 GRR2440 pKa = 11.84 THH2442 pKa = 6.02 HH2443 pKa = 6.6 WFRR2446 pKa = 11.84 EE2447 pKa = 4.23 PEE2449 pKa = 4.82 AIDD2452 pKa = 3.78 YY2453 pKa = 9.8 NQEE2456 pKa = 3.87 DD2457 pKa = 3.94 MTNTVTSALEE2467 pKa = 3.75 EE2468 pKa = 4.3 ACFHH2472 pKa = 6.8 GEE2474 pKa = 4.01 RR2475 pKa = 11.84 AFNLMKK2481 pKa = 10.27 QHH2483 pKa = 7.09 LFHH2486 pKa = 6.77 KK2487 pKa = 9.76 CKK2489 pKa = 10.03 MYY2491 pKa = 10.69 GIPLHH2496 pKa = 6.24 PTLSYY2501 pKa = 10.22 TFRR2504 pKa = 11.84 DD2505 pKa = 3.34 TLARR2509 pKa = 11.84 KK2510 pKa = 9.38 DD2511 pKa = 3.7 LNFLKK2516 pKa = 10.44 FIPDD2520 pKa = 3.2 SDD2522 pKa = 4.16 APEE2525 pKa = 3.41 IWGFVPRR2532 pKa = 11.84 QQ2533 pKa = 3.13
Molecular weight: 289.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.521
IPC2_protein 6.693
IPC_protein 6.634
Toseland 6.678
ProMoST 7.146
Dawson 7.161
Bjellqvist 7.337
Wikipedia 7.132
Rodwell 7.161
Grimsley 6.795
Solomon 7.161
Lehninger 7.176
Nozaki 7.614
DTASelect 7.483
Thurlkill 7.527
EMBOSS 7.512
Sillero 7.629
Patrickios 4.139
IPC_peptide 7.176
IPC2_peptide 7.044
IPC2.peptide.svr19 6.882
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3326
225
2533
1108.7
125.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.524 ± 1.259
1.984 ± 0.623
5.442 ± 0.517
5.713 ± 1.183
5.622 ± 0.352
5.201 ± 0.566
2.886 ± 0.273
5.803 ± 0.651
6.795 ± 1.56
8.148 ± 0.975
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.255 ± 0.221
4.991 ± 0.975
5.262 ± 0.289
4.57 ± 0.946
3.939 ± 0.487
7.547 ± 0.752
6.103 ± 1.104
6.254 ± 0.55
1.263 ± 0.012
3.698 ± 0.636
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here