Beihai shrimp virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE77|A0A1L3KE77_9VIRU Uncharacterized protein OS=Beihai shrimp virus 5 OX=1922671 PE=4 SV=1
MM1 pKa = 7.1GRR3 pKa = 11.84EE4 pKa = 3.99LEE6 pKa = 4.26TRR8 pKa = 11.84SDD10 pKa = 3.87GSGTTNGPNKK20 pKa = 9.56FRR22 pKa = 11.84ACLDD26 pKa = 3.47TTHH29 pKa = 6.39QGSVFSTFGNSKK41 pKa = 10.1IGVSNLGHH49 pKa = 6.31AVSPAKK55 pKa = 10.4EE56 pKa = 3.87EE57 pKa = 4.24SGVLDD62 pKa = 3.29SRR64 pKa = 11.84GSGNVDD70 pKa = 2.69PWSATQRR77 pKa = 11.84TTGSICVFDD86 pKa = 4.36GNYY89 pKa = 10.15GGCEE93 pKa = 3.7TSTSGTYY100 pKa = 9.68RR101 pKa = 11.84EE102 pKa = 4.1QQRR105 pKa = 11.84IVYY108 pKa = 9.58EE109 pKa = 3.95SGTIGKK115 pKa = 9.71RR116 pKa = 11.84KK117 pKa = 9.1IEE119 pKa = 3.93RR120 pKa = 11.84FEE122 pKa = 4.04SCEE125 pKa = 3.75RR126 pKa = 11.84RR127 pKa = 11.84AGTSQDD133 pKa = 3.61NEE135 pKa = 4.34LKK137 pKa = 10.5NSEE140 pKa = 4.48NVGEE144 pKa = 4.4TCDD147 pKa = 3.49GRR149 pKa = 11.84QIPTRR154 pKa = 11.84WFGKK158 pKa = 10.11SEE160 pKa = 4.11TDD162 pKa = 3.2EE163 pKa = 4.4PTSSGQIGNASSCEE177 pKa = 3.89EE178 pKa = 3.95SSEE181 pKa = 4.49SFGTRR186 pKa = 11.84TTEE189 pKa = 3.93RR190 pKa = 11.84RR191 pKa = 11.84NDD193 pKa = 3.32GCQSTGCASIAQEE206 pKa = 4.0TTTQQEE212 pKa = 4.52TEE214 pKa = 4.3FVSDD218 pKa = 3.94CSSEE222 pKa = 3.91RR223 pKa = 11.84RR224 pKa = 11.84SSIGSPCGFAPAGDD238 pKa = 4.15GEE240 pKa = 5.1PIFARR245 pKa = 11.84RR246 pKa = 11.84DD247 pKa = 3.43QPTMGRR253 pKa = 11.84FAWLHH258 pKa = 4.94EE259 pKa = 4.16QAVSAGCEE267 pKa = 3.99PIADD271 pKa = 4.21KK272 pKa = 10.4STSSAFEE279 pKa = 3.83WPPYY283 pKa = 10.56GGDD286 pKa = 3.41SEE288 pKa = 4.54YY289 pKa = 11.16QSLCAHH295 pKa = 6.88AKK297 pKa = 9.85LRR299 pKa = 11.84ADD301 pKa = 3.09AHH303 pKa = 6.6SNARR307 pKa = 11.84TPTKK311 pKa = 10.44LEE313 pKa = 3.65RR314 pKa = 11.84SAAIAATYY322 pKa = 9.27RR323 pKa = 11.84ANSKK327 pKa = 9.68FSKK330 pKa = 9.86WSLPVDD336 pKa = 4.58FLSRR340 pKa = 11.84AHH342 pKa = 6.59LVRR345 pKa = 11.84VIRR348 pKa = 11.84ALDD351 pKa = 3.73FTSSPGLPICKK362 pKa = 9.58YY363 pKa = 10.47SPTISDD369 pKa = 4.35FLRR372 pKa = 11.84VDD374 pKa = 3.88PVTGLPDD381 pKa = 3.25RR382 pKa = 11.84EE383 pKa = 4.08RR384 pKa = 11.84EE385 pKa = 3.85DD386 pKa = 3.87RR387 pKa = 11.84LIEE390 pKa = 4.08EE391 pKa = 4.54VYY393 pKa = 10.3RR394 pKa = 11.84WLDD397 pKa = 3.35EE398 pKa = 4.71GDD400 pKa = 2.96IQYY403 pKa = 10.68YY404 pKa = 9.23IRR406 pKa = 11.84LFIKK410 pKa = 10.42YY411 pKa = 8.61EE412 pKa = 3.7PHH414 pKa = 6.57KK415 pKa = 10.45LSKK418 pKa = 10.46ISAKK422 pKa = 9.97RR423 pKa = 11.84FRR425 pKa = 11.84LIWSIPLVHH434 pKa = 6.71QIVDD438 pKa = 3.55HH439 pKa = 6.74LLFDD443 pKa = 3.99AQNDD447 pKa = 4.06RR448 pKa = 11.84EE449 pKa = 4.4VQTSSLSEE457 pKa = 4.0FKK459 pKa = 10.86GGSSFAKK466 pKa = 10.21GGCLDD471 pKa = 4.19YY472 pKa = 10.94FSHH475 pKa = 6.26TPGWHH480 pKa = 6.06LSADD484 pKa = 3.24KK485 pKa = 10.96SLWDD489 pKa = 3.54FTVPAWACEE498 pKa = 3.75DD499 pKa = 3.72DD500 pKa = 4.45LEE502 pKa = 4.49FRR504 pKa = 11.84SMCCLDD510 pKa = 3.35SALNPEE516 pKa = 4.61KK517 pKa = 10.62FKK519 pKa = 10.61IWLEE523 pKa = 4.06CAQRR527 pKa = 11.84RR528 pKa = 11.84YY529 pKa = 11.04NEE531 pKa = 4.42MFNLDD536 pKa = 3.84YY537 pKa = 10.85DD538 pKa = 4.52VKK540 pKa = 11.04SKK542 pKa = 9.05PTLIQLSDD550 pKa = 3.24GRR552 pKa = 11.84RR553 pKa = 11.84FLQLTKK559 pKa = 11.04GLMKK563 pKa = 10.25SGSVITLSANSHH575 pKa = 4.91MQSFFHH581 pKa = 6.9RR582 pKa = 11.84IAWSRR587 pKa = 11.84VSDD590 pKa = 4.01VPAPLIWSMGDD601 pKa = 3.27DD602 pKa = 3.24TTLRR606 pKa = 11.84VSRR609 pKa = 11.84DD610 pKa = 3.55FPTEE614 pKa = 3.81LYY616 pKa = 10.7LRR618 pKa = 11.84EE619 pKa = 4.0TSEE622 pKa = 3.97LGAIVKK628 pKa = 8.46FANLTVNEE636 pKa = 4.2YY637 pKa = 9.98EE638 pKa = 4.13FAGMIIKK645 pKa = 10.15QPMCLIPSKK654 pKa = 11.13NDD656 pKa = 2.82THH658 pKa = 6.74YY659 pKa = 11.36YY660 pKa = 10.28KK661 pKa = 11.11LLFEE665 pKa = 4.32TDD667 pKa = 3.08EE668 pKa = 4.61RR669 pKa = 11.84LLEE672 pKa = 4.14MLHH675 pKa = 6.8SYY677 pKa = 10.31QFLYY681 pKa = 10.88AADD684 pKa = 4.02NRR686 pKa = 11.84LKK688 pKa = 9.97TIQYY692 pKa = 9.89VLSLLPGGSEE702 pKa = 4.11RR703 pKa = 11.84IEE705 pKa = 3.9TPMYY709 pKa = 9.91LNQWLTEE716 pKa = 4.32PGSSS720 pKa = 3.57

Molecular weight: 80.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE78|A0A1L3KE78_9VIRU Uncharacterized protein OS=Beihai shrimp virus 5 OX=1922671 PE=4 SV=1
MM1 pKa = 7.15QPNGTVTVSTKK12 pKa = 9.03NKK14 pKa = 8.9KK15 pKa = 9.17RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84QKK20 pKa = 10.51RR21 pKa = 11.84KK22 pKa = 8.6NKK24 pKa = 8.84TVIQVVQKK32 pKa = 9.83PKK34 pKa = 8.29RR35 pKa = 11.84QRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84GLALNQAPVAAGPVTRR56 pKa = 11.84TTFTGPLITSGMDD69 pKa = 3.26YY70 pKa = 11.13LDD72 pKa = 5.18ADD74 pKa = 5.44LIPAKK79 pKa = 10.51VDD81 pKa = 3.44DD82 pKa = 4.27GTLIVDD88 pKa = 3.84KK89 pKa = 10.7LLNPSQLPGTHH100 pKa = 6.75FSAIAGGFDD109 pKa = 3.17RR110 pKa = 11.84YY111 pKa = 10.61QFTSLSVQVISRR123 pKa = 11.84VPTTVGGGYY132 pKa = 9.38IAALSSDD139 pKa = 3.39VVEE142 pKa = 4.23EE143 pKa = 4.28FEE145 pKa = 5.03NGVSLKK151 pKa = 9.4QHH153 pKa = 4.43TMAMEE158 pKa = 4.98GSKK161 pKa = 10.46GAPWWTSVSVTMGKK175 pKa = 7.73QTGEE179 pKa = 3.58WYY181 pKa = 10.85YY182 pKa = 11.5NSPQSNEE189 pKa = 3.8RR190 pKa = 11.84FKK192 pKa = 9.01TTQARR197 pKa = 11.84FLMVVDD203 pKa = 5.32GPPTNLSMNSSVQVTLLLKK222 pKa = 9.99WKK224 pKa = 10.42AKK226 pKa = 9.15FASPTGMAPRR236 pKa = 11.84TVQKK240 pKa = 8.91FTIPRR245 pKa = 11.84GTVFTSPNPTHH256 pKa = 7.27PYY258 pKa = 8.16YY259 pKa = 10.86GQYY262 pKa = 10.76LYY264 pKa = 11.37VPGTLSEE271 pKa = 4.08FWKK274 pKa = 10.56ASKK277 pKa = 10.39FSYY280 pKa = 10.57VYY282 pKa = 9.99IVVPGAEE289 pKa = 4.5YY290 pKa = 10.76KK291 pKa = 9.1RR292 pKa = 11.84TPPAEE297 pKa = 4.17SANVFAIQVGKK308 pKa = 8.98TSEE311 pKa = 4.25GSWTIGLWEE320 pKa = 4.56TIEE323 pKa = 4.19SCEE326 pKa = 4.43KK327 pKa = 10.7VDD329 pKa = 4.83FNAPGGRR336 pKa = 11.84SGILCNGDD344 pKa = 2.66WTLDD348 pKa = 3.32RR349 pKa = 11.84DD350 pKa = 3.56VDD352 pKa = 4.3FRR354 pKa = 11.84PLCPVTPKK362 pKa = 10.29KK363 pKa = 10.27RR364 pKa = 11.84SYY366 pKa = 11.46LNLSVSDD373 pKa = 4.39DD374 pKa = 3.93FTHH377 pKa = 7.01KK378 pKa = 10.57AARR381 pKa = 11.84NLINTEE387 pKa = 3.9RR388 pKa = 11.84EE389 pKa = 4.41SIIHH393 pKa = 7.0ADD395 pKa = 3.65AIGNRR400 pKa = 11.84SNKK403 pKa = 9.48LLQASTEE410 pKa = 4.0LLQNQMKK417 pKa = 7.97MTEE420 pKa = 4.23KK421 pKa = 10.38LSSLMEE427 pKa = 4.43SLTLTIPIIGTTLRR441 pKa = 11.84TMNVEE446 pKa = 4.19VPIPPASSSCDD457 pKa = 3.11DD458 pKa = 3.47STFSVVAEE466 pKa = 4.06EE467 pKa = 4.62TEE469 pKa = 3.92

Molecular weight: 51.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2066

469

877

688.7

77.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.002 ± 0.098

1.597 ± 0.408

5.131 ± 0.379

6.873 ± 0.742

4.211 ± 0.23

6.05 ± 0.685

2.033 ± 0.351

5.227 ± 0.424

6.196 ± 0.817

8.132 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.338

3.872 ± 0.277

5.663 ± 0.44

4.55 ± 0.462

5.566 ± 0.56

8.422 ± 1.32

7.406 ± 0.824

6.583 ± 1.038

1.404 ± 0.301

2.711 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski