Amino acid dipepetide frequency for Streptococcus phage IPP25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.617AlaAla: 2.617 ± 0.751
0.28AlaCys: 0.28 ± 0.142
5.234AlaAsp: 5.234 ± 0.489
5.327AlaGlu: 5.327 ± 0.533
2.056AlaPhe: 2.056 ± 0.415
3.832AlaGly: 3.832 ± 0.676
0.841AlaHis: 0.841 ± 0.257
4.766AlaIle: 4.766 ± 0.61
5.327AlaLys: 5.327 ± 0.733
5.234AlaLeu: 5.234 ± 0.757
1.495AlaMet: 1.495 ± 0.401
4.112AlaAsn: 4.112 ± 0.522
0.935AlaPro: 0.935 ± 0.373
2.056AlaGln: 2.056 ± 0.548
2.991AlaArg: 2.991 ± 0.543
3.925AlaSer: 3.925 ± 0.577
4.486AlaThr: 4.486 ± 0.653
4.393AlaVal: 4.393 ± 0.824
0.841AlaTrp: 0.841 ± 0.251
1.963AlaTyr: 1.963 ± 0.359
0.0AlaXaa: 0.0 ± 0.0
Cys
0.093CysAla: 0.093 ± 0.094
0.0CysCys: 0.0 ± 0.0
0.374CysAsp: 0.374 ± 0.166
0.28CysGlu: 0.28 ± 0.149
0.0CysPhe: 0.0 ± 0.0
0.093CysGly: 0.093 ± 0.091
0.093CysHis: 0.093 ± 0.086
0.374CysIle: 0.374 ± 0.223
0.374CysLys: 0.374 ± 0.18
0.841CysLeu: 0.841 ± 0.257
0.093CysMet: 0.093 ± 0.094
0.374CysAsn: 0.374 ± 0.202
0.187CysPro: 0.187 ± 0.121
0.093CysGln: 0.093 ± 0.078
0.28CysArg: 0.28 ± 0.178
0.374CysSer: 0.374 ± 0.218
0.093CysThr: 0.093 ± 0.096
0.28CysVal: 0.28 ± 0.164
0.187CysTrp: 0.187 ± 0.101
0.467CysTyr: 0.467 ± 0.274
0.0CysXaa: 0.0 ± 0.0
Asp
3.364AspAla: 3.364 ± 0.386
0.187AspCys: 0.187 ± 0.146
4.393AspAsp: 4.393 ± 0.698
4.112AspGlu: 4.112 ± 0.571
3.832AspPhe: 3.832 ± 0.531
5.514AspGly: 5.514 ± 0.949
1.028AspHis: 1.028 ± 0.294
5.234AspIle: 5.234 ± 0.733
5.234AspLys: 5.234 ± 0.651
5.701AspLeu: 5.701 ± 0.885
1.869AspMet: 1.869 ± 0.372
4.299AspAsn: 4.299 ± 0.632
1.589AspPro: 1.589 ± 0.397
1.215AspGln: 1.215 ± 0.375
2.43AspArg: 2.43 ± 0.525
3.832AspSer: 3.832 ± 0.51
2.804AspThr: 2.804 ± 0.435
3.551AspVal: 3.551 ± 0.633
0.748AspTrp: 0.748 ± 0.286
3.178AspTyr: 3.178 ± 0.483
0.0AspXaa: 0.0 ± 0.0
Glu
4.766GluAla: 4.766 ± 0.628
0.28GluCys: 0.28 ± 0.143
3.178GluAsp: 3.178 ± 0.475
5.888GluGlu: 5.888 ± 0.921
2.523GluPhe: 2.523 ± 0.407
4.299GluGly: 4.299 ± 0.628
1.308GluHis: 1.308 ± 0.354
6.916GluIle: 6.916 ± 1.081
6.542GluLys: 6.542 ± 0.865
7.944GluLeu: 7.944 ± 0.908
2.056GluMet: 2.056 ± 0.502
4.86GluAsn: 4.86 ± 0.718
1.869GluPro: 1.869 ± 0.43
3.551GluGln: 3.551 ± 0.466
3.551GluArg: 3.551 ± 0.613
4.112GluSer: 4.112 ± 0.558
4.86GluThr: 4.86 ± 0.646
5.421GluVal: 5.421 ± 0.946
1.215GluTrp: 1.215 ± 0.329
3.551GluTyr: 3.551 ± 0.499
0.0GluXaa: 0.0 ± 0.0
Phe
2.243PheAla: 2.243 ± 0.494
0.093PheCys: 0.093 ± 0.098
2.991PheAsp: 2.991 ± 0.55
4.393PheGlu: 4.393 ± 0.814
2.523PhePhe: 2.523 ± 0.521
2.804PheGly: 2.804 ± 0.657
0.374PheHis: 0.374 ± 0.163
1.963PheIle: 1.963 ± 0.483
3.084PheLys: 3.084 ± 0.378
3.084PheLeu: 3.084 ± 0.386
0.841PheMet: 0.841 ± 0.233
2.897PheAsn: 2.897 ± 0.538
0.748PhePro: 0.748 ± 0.245
1.495PheGln: 1.495 ± 0.325
1.589PheArg: 1.589 ± 0.397
2.336PheSer: 2.336 ± 0.463
2.523PheThr: 2.523 ± 0.414
2.804PheVal: 2.804 ± 0.441
0.561PheTrp: 0.561 ± 0.214
1.869PheTyr: 1.869 ± 0.347
0.0PheXaa: 0.0 ± 0.0
Gly
4.206GlyAla: 4.206 ± 0.911
0.28GlyCys: 0.28 ± 0.176
3.364GlyAsp: 3.364 ± 0.531
3.925GlyGlu: 3.925 ± 0.587
3.084GlyPhe: 3.084 ± 0.475
3.551GlyGly: 3.551 ± 0.715
1.215GlyHis: 1.215 ± 0.282
5.14GlyIle: 5.14 ± 0.725
5.888GlyLys: 5.888 ± 0.817
4.953GlyLeu: 4.953 ± 0.732
1.869GlyMet: 1.869 ± 0.486
2.617GlyAsn: 2.617 ± 0.392
0.561GlyPro: 0.561 ± 0.215
2.43GlyGln: 2.43 ± 0.533
2.991GlyArg: 2.991 ± 0.488
2.897GlySer: 2.897 ± 0.723
2.897GlyThr: 2.897 ± 0.605
3.178GlyVal: 3.178 ± 0.567
1.308GlyTrp: 1.308 ± 0.38
2.897GlyTyr: 2.897 ± 0.435
0.0GlyXaa: 0.0 ± 0.0
His
0.841HisAla: 0.841 ± 0.264
0.0HisCys: 0.0 ± 0.0
1.308HisAsp: 1.308 ± 0.278
1.028HisGlu: 1.028 ± 0.272
0.935HisPhe: 0.935 ± 0.299
1.028HisGly: 1.028 ± 0.288
0.374HisHis: 0.374 ± 0.167
1.215HisIle: 1.215 ± 0.284
0.935HisLys: 0.935 ± 0.282
1.308HisLeu: 1.308 ± 0.322
0.561HisMet: 0.561 ± 0.206
1.121HisAsn: 1.121 ± 0.276
0.561HisPro: 0.561 ± 0.224
0.561HisGln: 0.561 ± 0.181
0.093HisArg: 0.093 ± 0.084
0.561HisSer: 0.561 ± 0.226
0.467HisThr: 0.467 ± 0.17
0.654HisVal: 0.654 ± 0.219
0.187HisTrp: 0.187 ± 0.129
0.467HisTyr: 0.467 ± 0.275
0.0HisXaa: 0.0 ± 0.0
Ile
5.701IleAla: 5.701 ± 0.682
0.467IleCys: 0.467 ± 0.182
5.701IleAsp: 5.701 ± 0.609
6.542IleGlu: 6.542 ± 0.838
2.43IlePhe: 2.43 ± 0.438
3.364IleGly: 3.364 ± 0.519
0.841IleHis: 0.841 ± 0.217
5.234IleIle: 5.234 ± 0.661
7.57IleLys: 7.57 ± 0.786
6.355IleLeu: 6.355 ± 0.737
1.869IleMet: 1.869 ± 0.325
3.832IleAsn: 3.832 ± 0.777
2.243IlePro: 2.243 ± 0.454
1.869IleGln: 1.869 ± 0.516
2.71IleArg: 2.71 ± 0.481
5.047IleSer: 5.047 ± 0.839
4.486IleThr: 4.486 ± 0.779
4.206IleVal: 4.206 ± 0.556
1.028IleTrp: 1.028 ± 0.422
2.804IleTyr: 2.804 ± 0.538
0.0IleXaa: 0.0 ± 0.0
Lys
5.327LysAla: 5.327 ± 0.701
0.28LysCys: 0.28 ± 0.148
4.579LysAsp: 4.579 ± 0.541
8.318LysGlu: 8.318 ± 0.747
3.271LysPhe: 3.271 ± 0.601
4.953LysGly: 4.953 ± 0.658
1.121LysHis: 1.121 ± 0.342
6.168LysIle: 6.168 ± 0.853
8.692LysLys: 8.692 ± 1.161
7.757LysLeu: 7.757 ± 0.923
2.71LysMet: 2.71 ± 0.536
5.421LysAsn: 5.421 ± 0.707
2.336LysPro: 2.336 ± 0.442
3.271LysGln: 3.271 ± 0.582
3.364LysArg: 3.364 ± 0.568
5.514LysSer: 5.514 ± 0.675
5.981LysThr: 5.981 ± 0.984
5.514LysVal: 5.514 ± 0.646
1.121LysTrp: 1.121 ± 0.261
2.804LysTyr: 2.804 ± 0.553
0.0LysXaa: 0.0 ± 0.0
Leu
6.729LeuAla: 6.729 ± 0.612
0.841LeuCys: 0.841 ± 0.311
7.477LeuAsp: 7.477 ± 0.836
8.037LeuGlu: 8.037 ± 0.852
2.804LeuPhe: 2.804 ± 0.538
4.579LeuGly: 4.579 ± 0.716
0.841LeuHis: 0.841 ± 0.287
5.794LeuIle: 5.794 ± 0.64
8.505LeuLys: 8.505 ± 0.934
6.916LeuLeu: 6.916 ± 0.847
1.963LeuMet: 1.963 ± 0.434
4.206LeuAsn: 4.206 ± 0.575
2.804LeuPro: 2.804 ± 0.544
3.738LeuGln: 3.738 ± 0.674
3.271LeuArg: 3.271 ± 0.544
5.234LeuSer: 5.234 ± 0.632
5.421LeuThr: 5.421 ± 0.802
4.766LeuVal: 4.766 ± 0.548
1.028LeuTrp: 1.028 ± 0.284
1.776LeuTyr: 1.776 ± 0.34
0.0LeuXaa: 0.0 ± 0.0
Met
1.682MetAla: 1.682 ± 0.377
0.187MetCys: 0.187 ± 0.11
1.589MetAsp: 1.589 ± 0.345
2.617MetGlu: 2.617 ± 0.427
0.935MetPhe: 0.935 ± 0.264
1.121MetGly: 1.121 ± 0.332
0.187MetHis: 0.187 ± 0.118
1.682MetIle: 1.682 ± 0.437
1.776MetLys: 1.776 ± 0.36
2.243MetLeu: 2.243 ± 0.382
0.561MetMet: 0.561 ± 0.203
1.495MetAsn: 1.495 ± 0.386
0.935MetPro: 0.935 ± 0.277
0.654MetGln: 0.654 ± 0.243
1.402MetArg: 1.402 ± 0.38
1.589MetSer: 1.589 ± 0.368
2.243MetThr: 2.243 ± 0.378
2.15MetVal: 2.15 ± 0.392
0.374MetTrp: 0.374 ± 0.185
0.467MetTyr: 0.467 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
4.112AsnAla: 4.112 ± 0.458
0.561AsnCys: 0.561 ± 0.257
3.458AsnAsp: 3.458 ± 0.678
4.579AsnGlu: 4.579 ± 0.644
2.336AsnPhe: 2.336 ± 0.441
4.86AsnGly: 4.86 ± 0.591
0.935AsnHis: 0.935 ± 0.253
5.047AsnIle: 5.047 ± 0.742
4.953AsnLys: 4.953 ± 0.61
5.327AsnLeu: 5.327 ± 0.685
1.308AsnMet: 1.308 ± 0.306
3.738AsnAsn: 3.738 ± 0.734
2.15AsnPro: 2.15 ± 0.459
2.523AsnGln: 2.523 ± 0.53
2.617AsnArg: 2.617 ± 0.505
3.364AsnSer: 3.364 ± 0.536
3.271AsnThr: 3.271 ± 0.47
2.617AsnVal: 2.617 ± 0.453
1.028AsnTrp: 1.028 ± 0.305
2.897AsnTyr: 2.897 ± 0.533
0.0AsnXaa: 0.0 ± 0.0
Pro
1.682ProAla: 1.682 ± 0.594
0.28ProCys: 0.28 ± 0.146
1.495ProAsp: 1.495 ± 0.322
3.084ProGlu: 3.084 ± 0.579
1.028ProPhe: 1.028 ± 0.379
1.402ProGly: 1.402 ± 0.305
0.467ProHis: 0.467 ± 0.206
2.523ProIle: 2.523 ± 0.452
2.43ProLys: 2.43 ± 0.452
1.215ProLeu: 1.215 ± 0.331
0.374ProMet: 0.374 ± 0.159
1.963ProAsn: 1.963 ± 0.434
0.467ProPro: 0.467 ± 0.203
0.748ProGln: 0.748 ± 0.215
0.841ProArg: 0.841 ± 0.273
1.682ProSer: 1.682 ± 0.323
1.308ProThr: 1.308 ± 0.314
1.776ProVal: 1.776 ± 0.396
0.093ProTrp: 0.093 ± 0.089
1.121ProTyr: 1.121 ± 0.303
0.0ProXaa: 0.0 ± 0.0
Gln
2.71GlnAla: 2.71 ± 0.487
0.093GlnCys: 0.093 ± 0.11
1.495GlnAsp: 1.495 ± 0.319
2.243GlnGlu: 2.243 ± 0.417
1.402GlnPhe: 1.402 ± 0.307
1.495GlnGly: 1.495 ± 0.261
0.748GlnHis: 0.748 ± 0.289
2.897GlnIle: 2.897 ± 0.46
3.738GlnLys: 3.738 ± 0.807
3.458GlnLeu: 3.458 ± 0.586
0.561GlnMet: 0.561 ± 0.229
2.804GlnAsn: 2.804 ± 0.482
0.841GlnPro: 0.841 ± 0.242
1.869GlnGln: 1.869 ± 0.559
2.15GlnArg: 2.15 ± 0.368
2.243GlnSer: 2.243 ± 0.384
2.336GlnThr: 2.336 ± 0.355
2.243GlnVal: 2.243 ± 0.407
0.28GlnTrp: 0.28 ± 0.137
1.402GlnTyr: 1.402 ± 0.324
0.0GlnXaa: 0.0 ± 0.0
Arg
1.308ArgAla: 1.308 ± 0.299
0.374ArgCys: 0.374 ± 0.162
2.336ArgAsp: 2.336 ± 0.424
2.991ArgGlu: 2.991 ± 0.622
1.215ArgPhe: 1.215 ± 0.363
2.15ArgGly: 2.15 ± 0.451
0.935ArgHis: 0.935 ± 0.301
3.084ArgIle: 3.084 ± 0.538
3.458ArgLys: 3.458 ± 0.736
4.393ArgLeu: 4.393 ± 0.795
1.682ArgMet: 1.682 ± 0.378
3.271ArgAsn: 3.271 ± 0.583
0.748ArgPro: 0.748 ± 0.241
1.495ArgGln: 1.495 ± 0.391
2.523ArgArg: 2.523 ± 0.63
2.056ArgSer: 2.056 ± 0.41
3.271ArgThr: 3.271 ± 0.588
2.336ArgVal: 2.336 ± 0.527
0.935ArgTrp: 0.935 ± 0.347
1.776ArgTyr: 1.776 ± 0.387
0.0ArgXaa: 0.0 ± 0.0
Ser
3.271SerAla: 3.271 ± 0.522
0.093SerCys: 0.093 ± 0.095
3.645SerAsp: 3.645 ± 0.598
3.645SerGlu: 3.645 ± 0.676
1.869SerPhe: 1.869 ± 0.477
2.991SerGly: 2.991 ± 0.612
0.935SerHis: 0.935 ± 0.261
4.766SerIle: 4.766 ± 0.643
5.981SerLys: 5.981 ± 0.9
5.981SerLeu: 5.981 ± 0.694
1.495SerMet: 1.495 ± 0.371
3.832SerAsn: 3.832 ± 0.521
1.495SerPro: 1.495 ± 0.422
2.991SerGln: 2.991 ± 0.607
2.71SerArg: 2.71 ± 0.486
4.112SerSer: 4.112 ± 0.784
3.084SerThr: 3.084 ± 0.572
2.804SerVal: 2.804 ± 0.471
0.561SerTrp: 0.561 ± 0.175
3.178SerTyr: 3.178 ± 0.469
0.0SerXaa: 0.0 ± 0.0
Thr
4.673ThrAla: 4.673 ± 0.792
0.187ThrCys: 0.187 ± 0.127
4.019ThrAsp: 4.019 ± 0.604
4.579ThrGlu: 4.579 ± 0.747
2.804ThrPhe: 2.804 ± 0.486
5.14ThrGly: 5.14 ± 0.823
0.654ThrHis: 0.654 ± 0.257
5.14ThrIle: 5.14 ± 0.657
4.299ThrLys: 4.299 ± 0.667
4.393ThrLeu: 4.393 ± 0.626
1.682ThrMet: 1.682 ± 0.352
3.925ThrAsn: 3.925 ± 0.5
2.336ThrPro: 2.336 ± 0.558
1.963ThrGln: 1.963 ± 0.385
1.682ThrArg: 1.682 ± 0.413
2.991ThrSer: 2.991 ± 0.579
4.579ThrThr: 4.579 ± 0.73
3.738ThrVal: 3.738 ± 0.639
0.561ThrTrp: 0.561 ± 0.235
2.15ThrTyr: 2.15 ± 0.46
0.0ThrXaa: 0.0 ± 0.0
Val
4.112ValAla: 4.112 ± 0.599
0.187ValCys: 0.187 ± 0.124
3.738ValAsp: 3.738 ± 0.456
3.925ValGlu: 3.925 ± 0.63
2.71ValPhe: 2.71 ± 0.389
4.112ValGly: 4.112 ± 0.698
0.374ValHis: 0.374 ± 0.168
4.206ValIle: 4.206 ± 0.565
5.14ValLys: 5.14 ± 0.629
4.019ValLeu: 4.019 ± 0.529
1.589ValMet: 1.589 ± 0.338
3.832ValAsn: 3.832 ± 0.767
1.402ValPro: 1.402 ± 0.283
1.963ValGln: 1.963 ± 0.396
2.243ValArg: 2.243 ± 0.364
4.019ValSer: 4.019 ± 0.573
4.579ValThr: 4.579 ± 0.584
4.112ValVal: 4.112 ± 0.766
0.374ValTrp: 0.374 ± 0.198
2.243ValTyr: 2.243 ± 0.505
0.0ValXaa: 0.0 ± 0.0
Trp
0.654TrpAla: 0.654 ± 0.224
0.187TrpCys: 0.187 ± 0.131
0.748TrpAsp: 0.748 ± 0.252
1.028TrpGlu: 1.028 ± 0.262
0.935TrpPhe: 0.935 ± 0.241
0.28TrpGly: 0.28 ± 0.163
0.187TrpHis: 0.187 ± 0.118
0.467TrpIle: 0.467 ± 0.186
0.841TrpLys: 0.841 ± 0.27
1.215TrpLeu: 1.215 ± 0.402
0.467TrpMet: 0.467 ± 0.183
0.841TrpAsn: 0.841 ± 0.351
0.187TrpPro: 0.187 ± 0.113
0.748TrpGln: 0.748 ± 0.284
0.841TrpArg: 0.841 ± 0.234
0.841TrpSer: 0.841 ± 0.323
0.748TrpThr: 0.748 ± 0.195
0.935TrpVal: 0.935 ± 0.259
0.187TrpTrp: 0.187 ± 0.159
0.748TrpTyr: 0.748 ± 0.407
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.617TyrAla: 2.617 ± 0.561
0.093TyrCys: 0.093 ± 0.078
2.991TyrAsp: 2.991 ± 0.459
2.15TyrGlu: 2.15 ± 0.539
2.523TyrPhe: 2.523 ± 0.489
1.682TyrGly: 1.682 ± 0.432
0.748TyrHis: 0.748 ± 0.309
1.682TyrIle: 1.682 ± 0.418
3.738TyrLys: 3.738 ± 0.392
4.393TyrLeu: 4.393 ± 0.684
0.748TyrMet: 0.748 ± 0.313
2.243TyrAsn: 2.243 ± 0.423
1.589TyrPro: 1.589 ± 0.39
1.776TyrGln: 1.776 ± 0.401
2.056TyrArg: 2.056 ± 0.414
2.71TyrSer: 2.71 ± 0.66
2.056TyrThr: 2.056 ± 0.427
1.495TyrVal: 1.495 ± 0.355
0.467TyrTrp: 0.467 ± 0.187
1.776TyrTyr: 1.776 ± 0.584
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (10701 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski