Rhinopithecus roxellana (Golden snub-nosed monkey) (Pygathrix roxellana)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44577 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K6RK38|A0A2K6RK38_RHIRO 40S ribosomal protein S9 OS=Rhinopithecus roxellana OX=61622 PE=3 SV=1
MM1 pKa = 7.51 SFLKK5 pKa = 10.56 SFPPPGPAEE14 pKa = 4.1 GLLWQQPDD22 pKa = 3.61 TEE24 pKa = 4.45 AVLNGKK30 pKa = 8.91 GLGTGTLYY38 pKa = 10.36 IAEE41 pKa = 4.77 SRR43 pKa = 11.84 LSWLDD48 pKa = 3.16 GSGLGFSLEE57 pKa = 4.09 YY58 pKa = 9.04 PTISLHH64 pKa = 5.94 ALSRR68 pKa = 11.84 DD69 pKa = 3.33 RR70 pKa = 11.84 SDD72 pKa = 4.01 CLGEE76 pKa = 3.91 HH77 pKa = 7.02 LYY79 pKa = 11.73 VMVNAKK85 pKa = 10.28 FEE87 pKa = 4.38 EE88 pKa = 4.69 EE89 pKa = 4.23 SKK91 pKa = 10.66 EE92 pKa = 3.94 PAMFTAMCEE101 pKa = 4.33 CQALHH106 pKa = 7.39 PDD108 pKa = 4.25 PEE110 pKa = 6.21 DD111 pKa = 4.16 EE112 pKa = 6.53 DD113 pKa = 5.1 SDD115 pKa = 5.45 DD116 pKa = 4.29 YY117 pKa = 11.95 DD118 pKa = 4.07 GEE120 pKa = 4.56 EE121 pKa = 4.23 YY122 pKa = 10.88 DD123 pKa = 4.24 GDD125 pKa = 3.54 IPTFYY130 pKa = 9.98 TYY132 pKa = 11.09 EE133 pKa = 4.17 EE134 pKa = 4.62 GLSHH138 pKa = 6.9 LTAEE142 pKa = 4.71 GQATLEE148 pKa = 4.16 RR149 pKa = 11.84 LEE151 pKa = 4.8 GMLSQSVSSQYY162 pKa = 11.68 NMAGVRR168 pKa = 11.84 TEE170 pKa = 4.12 DD171 pKa = 3.21 SVRR174 pKa = 11.84 DD175 pKa = 3.8 YY176 pKa = 11.43 EE177 pKa = 5.89 DD178 pKa = 3.4 GMEE181 pKa = 4.14 VDD183 pKa = 3.82 TTPTVAGQFEE193 pKa = 4.58 DD194 pKa = 4.85 ADD196 pKa = 3.71 VDD198 pKa = 3.98 HH199 pKa = 7.2
Molecular weight: 21.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.91
IPC_protein 3.884
Toseland 3.694
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.605
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.151
Thurlkill 3.732
EMBOSS 3.77
Sillero 3.999
Patrickios 1.189
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.889
Protein with the highest isoelectric point:
>tr|A0A2K6Q292|A0A2K6Q292_RHIRO Uncharacterized protein OS=Rhinopithecus roxellana OX=61622 GN=LSMEM1 PE=4 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.12 WRR6 pKa = 11.84 KK7 pKa = 9.1 KK8 pKa = 9.32 RR9 pKa = 11.84 MRR11 pKa = 11.84 RR12 pKa = 11.84 LKK14 pKa = 10.08 RR15 pKa = 11.84 KK16 pKa = 8.21 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.46 MRR21 pKa = 11.84 QRR23 pKa = 11.84 SKK25 pKa = 11.41
Molecular weight: 3.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21114
23463
44577
23994639
12
34273
538.3
59.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.871 ± 0.015
2.188 ± 0.009
4.877 ± 0.008
7.235 ± 0.016
3.602 ± 0.008
6.441 ± 0.017
2.595 ± 0.008
4.415 ± 0.011
5.892 ± 0.017
9.842 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.165 ± 0.006
3.666 ± 0.01
6.167 ± 0.018
4.813 ± 0.015
5.618 ± 0.011
8.294 ± 0.015
5.332 ± 0.012
6.031 ± 0.014
1.2 ± 0.004
2.651 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here