Gammapapillomavirus 24
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2AM75|A0A2D2AM75_9PAPI Replication protein E1 OS=Gammapapillomavirus 24 OX=1961681 GN=E1 PE=3 SV=1
MM1 pKa = 7.62 EE2 pKa = 5.41 PRR4 pKa = 11.84 AKK6 pKa = 9.9 RR7 pKa = 11.84 QKK9 pKa = 10.17 RR10 pKa = 11.84 DD11 pKa = 3.45 SVEE14 pKa = 3.53 NLYY17 pKa = 10.18 RR18 pKa = 11.84 QCKK21 pKa = 8.4 LTGQCPDD28 pKa = 3.27 DD29 pKa = 4.54 VINKK33 pKa = 9.42 VEE35 pKa = 4.15 GNTLADD41 pKa = 3.99 RR42 pKa = 11.84 LLKK45 pKa = 10.5 ILGSVIYY52 pKa = 10.6 LGGLGIGTGRR62 pKa = 11.84 GSGGSTGYY70 pKa = 10.46 RR71 pKa = 11.84 PIGATTPRR79 pKa = 11.84 VTDD82 pKa = 3.47 STPIRR87 pKa = 11.84 PTVPVDD93 pKa = 3.3 PLGPTDD99 pKa = 4.24 VLPVDD104 pKa = 3.63 PAGPSIVPLTEE115 pKa = 3.78 SGIPEE120 pKa = 3.91 DD121 pKa = 4.43 TIIEE125 pKa = 4.33 TSGTAVTVNPTEE137 pKa = 4.19 PPVITNIDD145 pKa = 4.31 PISDD149 pKa = 3.57 VTGVEE154 pKa = 4.11 GQPTIITGEE163 pKa = 4.16 DD164 pKa = 3.31 DD165 pKa = 3.69 SVAVLDD171 pKa = 4.36 VQPSTPAPKK180 pKa = 9.96 RR181 pKa = 11.84 VTIGVRR187 pKa = 11.84 PRR189 pKa = 11.84 TSSPSEE195 pKa = 3.62 PTLYY199 pKa = 10.2 PIPEE203 pKa = 4.14 PAAPDD208 pKa = 3.34 FTVFVDD214 pKa = 4.21 AQAVGEE220 pKa = 4.53 TIGGEE225 pKa = 4.15 YY226 pKa = 9.98 IPLEE230 pKa = 4.4 EE231 pKa = 5.01 FSQIQNFEE239 pKa = 3.9 IEE241 pKa = 4.32 EE242 pKa = 4.05 QQTLSSTPEE251 pKa = 3.53 QRR253 pKa = 11.84 LNRR256 pKa = 11.84 AFQRR260 pKa = 11.84 ARR262 pKa = 11.84 DD263 pKa = 3.75 LYY265 pKa = 10.82 NRR267 pKa = 11.84 QITQIRR273 pKa = 11.84 TRR275 pKa = 11.84 NLDD278 pKa = 3.42 FLGAASRR285 pKa = 11.84 AVQFEE290 pKa = 4.21 FEE292 pKa = 4.35 NPAFVDD298 pKa = 4.1 DD299 pKa = 3.74 VTLQFEE305 pKa = 4.18 QDD307 pKa = 3.6 VRR309 pKa = 11.84 DD310 pKa = 3.84 VAAAPDD316 pKa = 3.96 PDD318 pKa = 3.57 FRR320 pKa = 11.84 DD321 pKa = 3.0 IVTLHH326 pKa = 6.93 RR327 pKa = 11.84 ARR329 pKa = 11.84 YY330 pKa = 7.11 STTDD334 pKa = 2.98 TGRR337 pKa = 11.84 VRR339 pKa = 11.84 VSRR342 pKa = 11.84 LGKK345 pKa = 9.98 RR346 pKa = 11.84 GTIVTRR352 pKa = 11.84 SGAQIGEE359 pKa = 4.3 NVHH362 pKa = 6.74 FYY364 pKa = 11.29 YY365 pKa = 10.63 DD366 pKa = 3.37 VSTIGSDD373 pKa = 3.03 AADD376 pKa = 4.67 AIEE379 pKa = 4.85 LSTIGQHH386 pKa = 6.19 SGDD389 pKa = 3.93 SAIVDD394 pKa = 3.93 ALAEE398 pKa = 4.21 SSFINPIEE406 pKa = 4.54 NPDD409 pKa = 3.5 TTYY412 pKa = 11.17 TEE414 pKa = 4.76 ADD416 pKa = 3.53 LLDD419 pKa = 3.98 TQVEE423 pKa = 4.44 DD424 pKa = 4.06 FDD426 pKa = 4.71 GLHH429 pKa = 6.6 LVLTSSGRR437 pKa = 11.84 RR438 pKa = 11.84 GDD440 pKa = 3.77 VYY442 pKa = 10.94 SIPTLPPGTTLRR454 pKa = 11.84 VFVDD458 pKa = 3.69 DD459 pKa = 3.76 YY460 pKa = 11.89 GRR462 pKa = 11.84 DD463 pKa = 3.49 LFVSYY468 pKa = 9.96 PVSHH472 pKa = 7.03 TKK474 pKa = 9.95 TDD476 pKa = 3.02 ILLPDD481 pKa = 3.85 EE482 pKa = 4.93 ALHH485 pKa = 5.98 PLEE488 pKa = 5.24 PPVLLDD494 pKa = 4.66 AYY496 pKa = 11.12 SSDD499 pKa = 4.48 YY500 pKa = 10.76 YY501 pKa = 10.81 LHH503 pKa = 6.96 PSLWSRR509 pKa = 11.84 RR510 pKa = 11.84 KK511 pKa = 9.64 RR512 pKa = 11.84 KK513 pKa = 10.01 RR514 pKa = 11.84 SDD516 pKa = 2.5 IYY518 pKa = 11.62 NFFTDD523 pKa = 3.75 GTVDD527 pKa = 3.15 ATEE530 pKa = 3.84 WW531 pKa = 3.26
Molecular weight: 58.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.557
IPC2_protein 4.469
IPC_protein 4.457
Toseland 4.279
ProMoST 4.584
Dawson 4.418
Bjellqvist 4.571
Wikipedia 4.329
Rodwell 4.291
Grimsley 4.177
Solomon 4.418
Lehninger 4.38
Nozaki 4.533
DTASelect 4.749
Thurlkill 4.291
EMBOSS 4.342
Sillero 4.571
Patrickios 3.961
IPC_peptide 4.418
IPC2_peptide 4.558
IPC2.peptide.svr19 4.518
Protein with the highest isoelectric point:
>tr|A0A2D2AM89|A0A2D2AM89_9PAPI Protein E7 OS=Gammapapillomavirus 24 OX=1961681 GN=E7 PE=3 SV=1
MM1 pKa = 7.53 EE2 pKa = 4.87 WLTDD6 pKa = 3.37 RR7 pKa = 11.84 FNAVQEE13 pKa = 4.27 TLLNLIEE20 pKa = 5.37 QGAQDD25 pKa = 4.68 LDD27 pKa = 3.89 SQIEE31 pKa = 4.04 YY32 pKa = 9.14 WNNVRR37 pKa = 11.84 KK38 pKa = 10.07 EE39 pKa = 4.14 NVYY42 pKa = 9.03 MYY44 pKa = 9.06 YY45 pKa = 10.86 AKK47 pKa = 10.48 RR48 pKa = 11.84 EE49 pKa = 4.13 GLTHH53 pKa = 7.23 IGLQPLPVPAVSEE66 pKa = 4.36 YY67 pKa = 10.27 KK68 pKa = 10.53 AKK70 pKa = 10.38 QAIHH74 pKa = 6.71 LVLLLQSLKK83 pKa = 10.22 KK84 pKa = 10.44 SPYY87 pKa = 8.68 ATEE90 pKa = 3.86 SWTLQNTSEE99 pKa = 4.27 EE100 pKa = 4.74 LINTQPKK107 pKa = 10.61 DD108 pKa = 3.61 CFKK111 pKa = 10.38 KK112 pKa = 9.74 HH113 pKa = 6.45 PYY115 pKa = 8.21 TVEE118 pKa = 3.4 VWFDD122 pKa = 3.18 NDD124 pKa = 3.47 RR125 pKa = 11.84 NNSYY129 pKa = 10.75 PYY131 pKa = 10.25 INWDD135 pKa = 3.5 AIYY138 pKa = 10.67 YY139 pKa = 9.85 QDD141 pKa = 5.31 SQEE144 pKa = 4.16 KK145 pKa = 6.18 WHH147 pKa = 6.44 KK148 pKa = 10.75 VPGLVDD154 pKa = 3.71 YY155 pKa = 11.46 NGLYY159 pKa = 9.67 YY160 pKa = 10.94 NEE162 pKa = 4.3 VGGDD166 pKa = 2.95 RR167 pKa = 11.84 VYY169 pKa = 10.36 FTIFDD174 pKa = 3.9 TDD176 pKa = 3.34 AQKK179 pKa = 11.07 YY180 pKa = 7.97 GQTGMWTVHH189 pKa = 6.53 FKK191 pKa = 10.91 RR192 pKa = 11.84 KK193 pKa = 7.85 TLVAPISSSKK203 pKa = 10.37 QSSAYY208 pKa = 10.02 SGKK211 pKa = 8.23 TPTSVSSSSKK221 pKa = 9.66 DD222 pKa = 3.3 TLPTKK227 pKa = 10.07 EE228 pKa = 4.14 SPRR231 pKa = 11.84 RR232 pKa = 11.84 LQRR235 pKa = 11.84 PEE237 pKa = 4.02 VGSSTGEE244 pKa = 3.83 TTAVRR249 pKa = 11.84 RR250 pKa = 11.84 RR251 pKa = 11.84 RR252 pKa = 11.84 RR253 pKa = 11.84 EE254 pKa = 3.73 QGEE257 pKa = 4.26 SSTDD261 pKa = 3.56 GEE263 pKa = 4.42 PTTTSKK269 pKa = 10.52 RR270 pKa = 11.84 RR271 pKa = 11.84 RR272 pKa = 11.84 GGGGGGGTGRR282 pKa = 11.84 LGVSPEE288 pKa = 4.15 EE289 pKa = 4.23 VGSRR293 pKa = 11.84 HH294 pKa = 5.55 HH295 pKa = 6.53 SATGRR300 pKa = 11.84 HH301 pKa = 4.64 RR302 pKa = 11.84 TRR304 pKa = 11.84 LEE306 pKa = 3.62 QLKK309 pKa = 10.45 EE310 pKa = 3.9 EE311 pKa = 4.59 ARR313 pKa = 11.84 DD314 pKa = 3.53 PPIIIVTGPANSLKK328 pKa = 9.67 CWRR331 pKa = 11.84 YY332 pKa = 9.64 RR333 pKa = 11.84 KK334 pKa = 9.51 QNSNAFPFLAVSTIFTWVGGCGSAEE359 pKa = 4.0 QARR362 pKa = 11.84 MLIAFNTNEE371 pKa = 4.42 EE372 pKa = 3.9 RR373 pKa = 11.84 TQFIKK378 pKa = 10.85 YY379 pKa = 8.54 VQFPKK384 pKa = 9.52 QTTFALGQLDD394 pKa = 3.7 KK395 pKa = 11.57 LL396 pKa = 4.16
Molecular weight: 45.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.638
IPC2_protein 8.624
IPC_protein 8.551
Toseland 9.077
ProMoST 8.975
Dawson 9.414
Bjellqvist 9.209
Wikipedia 9.619
Rodwell 9.575
Grimsley 9.502
Solomon 9.472
Lehninger 9.414
Nozaki 9.209
DTASelect 9.165
Thurlkill 9.253
EMBOSS 9.531
Sillero 9.385
Patrickios 4.584
IPC_peptide 9.458
IPC2_peptide 7.834
IPC2.peptide.svr19 7.699
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2448
95
607
349.7
39.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.596 ± 0.495
2.206 ± 0.607
6.985 ± 0.748
6.25 ± 0.512
4.248 ± 0.415
5.76 ± 0.652
1.838 ± 0.227
5.27 ± 0.466
5.351 ± 0.905
8.864 ± 0.488
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.471 ± 0.322
4.453 ± 0.454
5.964 ± 1.017
5.351 ± 0.417
5.882 ± 0.692
6.618 ± 0.388
6.618 ± 0.761
6.168 ± 0.448
1.225 ± 0.284
3.881 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here