Sorex minutus polyomavirus 1
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A223PYN7|A0A223PYN7_9POLY small T antigen OS=Sorex minutus polyomavirus 1 OX=2560771 GN=STAg PE=4 SV=1
MM1 pKa = 7.44 GGVISLAVEE10 pKa = 3.99 LAGAISSLVAATEE23 pKa = 4.09 LTAEE27 pKa = 4.97 AILTGEE33 pKa = 4.12 ALAAIEE39 pKa = 4.52 SEE41 pKa = 4.52 VASMVLVEE49 pKa = 5.18 GFAEE53 pKa = 4.39 ADD55 pKa = 3.12 ALAALGLTAEE65 pKa = 4.47 HH66 pKa = 6.58 MSMLTAIPGMVEE78 pKa = 3.74 QLASTGLLIQTLSGAAALFTAGIKK102 pKa = 10.43 LSQHH106 pKa = 5.26 EE107 pKa = 4.38 VSLVNRR113 pKa = 11.84 NTNMALQIWRR123 pKa = 11.84 PDD125 pKa = 3.02 LWEE128 pKa = 5.22 LILPGFRR135 pKa = 11.84 HH136 pKa = 6.01 FEE138 pKa = 3.72 WGFDD142 pKa = 3.55 VLGDD146 pKa = 3.59 WGHH149 pKa = 6.22 SLVSAVGRR157 pKa = 11.84 YY158 pKa = 6.27 VWEE161 pKa = 3.97 MLLTEE166 pKa = 4.01 SRR168 pKa = 11.84 RR169 pKa = 11.84 RR170 pKa = 11.84 LGMLGGNLIGEE181 pKa = 4.86 GRR183 pKa = 11.84 RR184 pKa = 11.84 QAAEE188 pKa = 3.65 QVAQLLEE195 pKa = 3.93 GARR198 pKa = 11.84 WVVTSPYY205 pKa = 10.12 TGLQNYY211 pKa = 7.97 YY212 pKa = 10.58 RR213 pKa = 11.84 SLPPMNVIQMRR224 pKa = 11.84 QQARR228 pKa = 11.84 LRR230 pKa = 11.84 GQKK233 pKa = 10.47 LIDD236 pKa = 3.54 YY237 pKa = 9.8 NRR239 pKa = 11.84 VEE241 pKa = 4.63 EE242 pKa = 4.39 IDD244 pKa = 3.9 PEE246 pKa = 4.32 SGEE249 pKa = 4.59 VIQKK253 pKa = 9.56 YY254 pKa = 9.74 GPPGGADD261 pKa = 3.2 QPVTPDD267 pKa = 2.75 WMLLMILGLYY277 pKa = 10.59 GDD279 pKa = 4.27 ITPLWGHH286 pKa = 5.71 VLEE289 pKa = 4.71 QEE291 pKa = 5.33 HH292 pKa = 6.65 ILSPKK297 pKa = 10.62 NMIEE301 pKa = 4.11 GPPAKK306 pKa = 9.77 KK307 pKa = 9.58 RR308 pKa = 11.84 RR309 pKa = 11.84 KK310 pKa = 8.08 MM311 pKa = 3.39
Molecular weight: 33.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.114
IPC2_protein 5.13
IPC_protein 5.054
Toseland 5.016
ProMoST 5.169
Dawson 5.029
Bjellqvist 5.156
Wikipedia 4.863
Rodwell 4.965
Grimsley 4.952
Solomon 5.029
Lehninger 4.978
Nozaki 5.156
DTASelect 5.245
Thurlkill 5.003
EMBOSS 4.914
Sillero 5.232
Patrickios 4.228
IPC_peptide 5.041
IPC2_peptide 5.232
IPC2.peptide.svr19 5.208
Protein with the highest isoelectric point:
>tr|A0A223PYN8|A0A223PYN8_9POLY Capsid protein VP1 OS=Sorex minutus polyomavirus 1 OX=2560771 GN=VP1 PE=3 SV=1
MM1 pKa = 7.84 DD2 pKa = 6.58 RR3 pKa = 11.84 IFTSADD9 pKa = 3.53 RR10 pKa = 11.84 QLLISLLRR18 pKa = 11.84 ISGDD22 pKa = 3.21 TFGNVPAMARR32 pKa = 11.84 AYY34 pKa = 9.7 KK35 pKa = 10.24 LAAKK39 pKa = 10.03 RR40 pKa = 11.84 LHH42 pKa = 6.69 PDD44 pKa = 2.68 KK45 pKa = 11.18 GGNEE49 pKa = 4.08 ADD51 pKa = 3.28 MKK53 pKa = 10.91 KK54 pKa = 10.73 LNEE57 pKa = 4.01 LWNKK61 pKa = 9.74 FKK63 pKa = 11.4 DD64 pKa = 3.4 EE65 pKa = 4.2 IYY67 pKa = 10.8 RR68 pKa = 11.84 MRR70 pKa = 11.84 EE71 pKa = 3.58 VKK73 pKa = 10.38 PCVNPVITCIVLGTCSIFALIRR95 pKa = 11.84 NTAQCMQNLLRR106 pKa = 11.84 CCTCICCLLFKK117 pKa = 8.29 QHH119 pKa = 6.96 RR120 pKa = 11.84 EE121 pKa = 3.63 LKK123 pKa = 7.36 ITYY126 pKa = 9.71 RR127 pKa = 11.84 RR128 pKa = 11.84 RR129 pKa = 11.84 CNVWGEE135 pKa = 4.32 CYY137 pKa = 10.58 CFFCYY142 pKa = 9.05 YY143 pKa = 9.35 TWFGVNCSLNAFTEE157 pKa = 4.07 WLILLKK163 pKa = 10.81 HH164 pKa = 6.36 LDD166 pKa = 3.17 WRR168 pKa = 11.84 LLKK171 pKa = 10.39 ISSAEE176 pKa = 3.78 LDD178 pKa = 3.48 SLGKK182 pKa = 10.61 YY183 pKa = 9.62 NFYY186 pKa = 10.8 EE187 pKa = 3.63 IFLIHH192 pKa = 6.62 FVKK195 pKa = 10.81 CC196 pKa = 3.56
Molecular weight: 23.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.133
IPC2_protein 8.068
IPC_protein 7.863
Toseland 7.351
ProMoST 8.492
Dawson 8.58
Bjellqvist 9.033
Wikipedia 8.492
Rodwell 8.624
Grimsley 7.293
Solomon 8.653
Lehninger 8.682
Nozaki 9.326
DTASelect 8.697
Thurlkill 8.756
EMBOSS 8.785
Sillero 9.121
Patrickios 3.897
IPC_peptide 8.653
IPC2_peptide 8.331
IPC2.peptide.svr19 8.468
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1777
195
709
355.4
40.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.359 ± 0.974
2.251 ± 0.995
5.402 ± 0.773
5.909 ± 0.605
3.995 ± 1.074
7.034 ± 1.244
2.026 ± 0.38
5.684 ± 0.513
5.965 ± 1.381
10.242 ± 1.104
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.151 ± 0.599
4.671 ± 0.648
5.29 ± 0.729
3.995 ± 0.497
5.684 ± 0.437
6.584 ± 0.934
5.177 ± 0.645
5.684 ± 0.879
1.688 ± 0.55
3.208 ± 0.485
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here