Pseudanabaena biceps PCC 7429

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Pseudanabaenaceae; Pseudanabaena; Pseudanabaena biceps

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4751 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L8MW26|L8MW26_9CYAN Uncharacterized protein OS=Pseudanabaena biceps PCC 7429 OX=927668 GN=Pse7429DRAFT_4566 PE=4 SV=1
TT1 pKa = 7.03DD2 pKa = 4.02TDD4 pKa = 3.44WQDD7 pKa = 3.04GHH9 pKa = 7.27DD10 pKa = 4.53GLDD13 pKa = 3.97GLDD16 pKa = 3.53SDD18 pKa = 5.65IALICNNHH26 pKa = 6.57LDD28 pKa = 3.88IFPHH32 pKa = 6.31PFLFRR37 pKa = 11.84TTVCISFTLPFFPFLVV53 pKa = 3.7

Molecular weight: 6.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L8N4R5|L8N4R5_9CYAN DNA (cytosine-5-)-methyltransferase OS=Pseudanabaena biceps PCC 7429 OX=927668 GN=Pse7429DRAFT_1756 PE=3 SV=1
MM1 pKa = 7.52TKK3 pKa = 8.95RR4 pKa = 11.84TLRR7 pKa = 11.84GSVRR11 pKa = 11.84KK12 pKa = 9.73KK13 pKa = 10.2KK14 pKa = 9.12RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MEE24 pKa = 4.32SPTGRR29 pKa = 11.84RR30 pKa = 11.84VIKK33 pKa = 10.24ARR35 pKa = 11.84RR36 pKa = 11.84SRR38 pKa = 11.84GRR40 pKa = 11.84VRR42 pKa = 11.84LTTVV46 pKa = 2.58

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4751

0

4751

1492453

30

5577

314.1

35.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.282 ± 0.037

0.952 ± 0.014

5.413 ± 0.037

6.087 ± 0.047

3.986 ± 0.026

6.383 ± 0.043

1.813 ± 0.02

7.326 ± 0.03

5.071 ± 0.033

10.7 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.97 ± 0.019

4.569 ± 0.035

4.381 ± 0.028

4.91 ± 0.035

5.162 ± 0.031

6.951 ± 0.037

5.474 ± 0.041

6.216 ± 0.028

1.329 ± 0.016

3.024 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski