Amino acid dipepetide frequency for Sphingobium japonicum BiD32

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.998AlaAla: 17.998 ± 0.163	1.155AlaCys: 1.155 ± 0.032	7.971AlaAsp: 7.971 ± 0.092	6.763AlaGlu: 6.763 ± 0.082	4.274AlaPhe: 4.274 ± 0.058	10.896AlaGly: 10.896 ± 0.102	2.46AlaHis: 2.46 ± 0.049	7.234AlaIle: 7.234 ± 0.074	4.042AlaLys: 4.042 ± 0.068	14.117AlaLeu: 14.117 ± 0.128	4.184AlaMet: 4.184 ± 0.057	3.153AlaAsn: 3.153 ± 0.063	6.282AlaPro: 6.282 ± 0.075	5.043AlaGln: 5.043 ± 0.064	9.673AlaArg: 9.673 ± 0.114	6.718AlaSer: 6.718 ± 0.071	6.677AlaThr: 6.677 ± 0.095	8.329AlaVal: 8.329 ± 0.087	1.657AlaTrp: 1.657 ± 0.032	2.688AlaTyr: 2.688 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	1.081CysAla: 1.081 ± 0.034	0.11CysCys: 0.11 ± 0.009	0.557CysAsp: 0.557 ± 0.02	0.396CysGlu: 0.396 ± 0.02	0.299CysPhe: 0.299 ± 0.016	0.88CysGly: 0.88 ± 0.026	0.236CysHis: 0.236 ± 0.013	0.384CysIle: 0.384 ± 0.014	0.197CysLys: 0.197 ± 0.013	0.756CysLeu: 0.756 ± 0.027	0.186CysMet: 0.186 ± 0.011	0.221CysAsn: 0.221 ± 0.012	0.488CysPro: 0.488 ± 0.021	0.229CysGln: 0.229 ± 0.014	0.62CysArg: 0.62 ± 0.027	0.475CysSer: 0.475 ± 0.02	0.424CysThr: 0.424 ± 0.019	0.551CysVal: 0.551 ± 0.024	0.151CysTrp: 0.151 ± 0.01	0.198CysTyr: 0.198 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.939AspAla: 7.939 ± 0.077	0.506AspCys: 0.506 ± 0.021	3.501AspAsp: 3.501 ± 0.059	3.285AspGlu: 3.285 ± 0.055	2.202AspPhe: 2.202 ± 0.039	5.83AspGly: 5.83 ± 0.083	1.492AspHis: 1.492 ± 0.042	3.189AspIle: 3.189 ± 0.052	1.789AspLys: 1.789 ± 0.041	5.947AspLeu: 5.947 ± 0.07	1.648AspMet: 1.648 ± 0.037	1.445AspAsn: 1.445 ± 0.039	3.764AspPro: 3.764 ± 0.057	2.019AspGln: 2.019 ± 0.041	4.919AspArg: 4.919 ± 0.069	2.505AspSer: 2.505 ± 0.051	2.552AspThr: 2.552 ± 0.047	3.968AspVal: 3.968 ± 0.061	1.118AspTrp: 1.118 ± 0.03	1.718AspTyr: 1.718 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	7.256GluAla: 7.256 ± 0.089	0.316GluCys: 0.316 ± 0.014	2.493GluAsp: 2.493 ± 0.045	2.749GluGlu: 2.749 ± 0.059	1.349GluPhe: 1.349 ± 0.029	4.2GluGly: 4.2 ± 0.063	1.014GluHis: 1.014 ± 0.032	2.924GluIle: 2.924 ± 0.05	1.844GluLys: 1.844 ± 0.043	4.615GluLeu: 4.615 ± 0.059	1.4GluMet: 1.4 ± 0.029	1.306GluAsn: 1.306 ± 0.031	2.288GluPro: 2.288 ± 0.045	2.162GluGln: 2.162 ± 0.04	4.656GluArg: 4.656 ± 0.079	2.22GluSer: 2.22 ± 0.046	2.939GluThr: 2.939 ± 0.045	3.148GluVal: 3.148 ± 0.055	0.702GluTrp: 0.702 ± 0.024	0.942GluTyr: 0.942 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.674PheAla: 4.674 ± 0.057	0.344PheCys: 0.344 ± 0.017	2.638PheAsp: 2.638 ± 0.05	1.798PheGlu: 1.798 ± 0.04	1.2PhePhe: 1.2 ± 0.031	3.461PheGly: 3.461 ± 0.055	0.78PheHis: 0.78 ± 0.024	1.463PheIle: 1.463 ± 0.033	0.848PheLys: 0.848 ± 0.027	3.122PheLeu: 3.122 ± 0.06	0.806PheMet: 0.806 ± 0.026	0.975PheAsn: 0.975 ± 0.029	1.523PhePro: 1.523 ± 0.039	0.914PheGln: 0.914 ± 0.022	2.272PheArg: 2.272 ± 0.043	2.091PheSer: 2.091 ± 0.044	2.012PheThr: 2.012 ± 0.053	2.434PheVal: 2.434 ± 0.041	0.501PheTrp: 0.501 ± 0.02	0.893PheTyr: 0.893 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	9.761GlyAla: 9.761 ± 0.104	0.836GlyCys: 0.836 ± 0.028	5.109GlyAsp: 5.109 ± 0.075	4.432GlyGlu: 4.432 ± 0.055	3.496GlyPhe: 3.496 ± 0.048	7.846GlyGly: 7.846 ± 0.107	1.945GlyHis: 1.945 ± 0.041	4.447GlyIle: 4.447 ± 0.057	3.363GlyLys: 3.363 ± 0.054	8.587GlyLeu: 8.587 ± 0.087	2.439GlyMet: 2.439 ± 0.045	2.311GlyAsn: 2.311 ± 0.059	3.504GlyPro: 3.504 ± 0.05	3.305GlyGln: 3.305 ± 0.052	6.308GlyArg: 6.308 ± 0.079	4.77GlySer: 4.77 ± 0.073	4.721GlyThr: 4.721 ± 0.073	6.202GlyVal: 6.202 ± 0.08	1.634GlyTrp: 1.634 ± 0.042	2.314GlyTyr: 2.314 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.416HisAla: 2.416 ± 0.048	0.246HisCys: 0.246 ± 0.013	1.309HisAsp: 1.309 ± 0.037	0.993HisGlu: 0.993 ± 0.031	0.846HisPhe: 0.846 ± 0.026	2.086HisGly: 2.086 ± 0.047	0.642HisHis: 0.642 ± 0.025	1.042HisIle: 1.042 ± 0.028	0.479HisLys: 0.479 ± 0.021	2.01HisLeu: 2.01 ± 0.042	0.552HisMet: 0.552 ± 0.022	0.465HisAsn: 0.465 ± 0.019	1.339HisPro: 1.339 ± 0.034	0.623HisGln: 0.623 ± 0.019	1.545HisArg: 1.545 ± 0.041	1.033HisSer: 1.033 ± 0.027	0.649HisThr: 0.649 ± 0.022	1.625HisVal: 1.625 ± 0.042	0.393HisTrp: 0.393 ± 0.017	0.627HisTyr: 0.627 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	8.213IleAla: 8.213 ± 0.081	0.499IleCys: 0.499 ± 0.021	4.107IleAsp: 4.107 ± 0.05	3.336IleGlu: 3.336 ± 0.056	1.761IlePhe: 1.761 ± 0.038	5.235IleGly: 5.235 ± 0.077	1.012IleHis: 1.012 ± 0.029	2.437IleIle: 2.437 ± 0.045	1.308IleLys: 1.308 ± 0.034	4.439IleLeu: 4.439 ± 0.058	1.081IleMet: 1.081 ± 0.03	1.437IleAsn: 1.437 ± 0.037	2.31IlePro: 2.31 ± 0.041	1.179IleGln: 1.179 ± 0.03	3.33IleArg: 3.33 ± 0.053	2.768IleSer: 2.768 ± 0.043	2.563IleThr: 2.563 ± 0.05	4.179IleVal: 4.179 ± 0.059	0.661IleTrp: 0.661 ± 0.02	1.009IleTyr: 1.009 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.187LysAla: 4.187 ± 0.066	0.157LysCys: 0.157 ± 0.011	1.649LysAsp: 1.649 ± 0.04	1.212LysGlu: 1.212 ± 0.034	0.795LysPhe: 0.795 ± 0.025	2.829LysGly: 2.829 ± 0.054	0.52LysHis: 0.52 ± 0.021	1.55LysIle: 1.55 ± 0.038	1.061LysLys: 1.061 ± 0.033	3.143LysLeu: 3.143 ± 0.052	0.779LysMet: 0.779 ± 0.023	0.712LysAsn: 0.712 ± 0.026	2.023LysPro: 2.023 ± 0.047	0.979LysGln: 0.979 ± 0.025	2.216LysArg: 2.216 ± 0.04	1.631LysSer: 1.631 ± 0.04	1.604LysThr: 1.604 ± 0.035	2.106LysVal: 2.106 ± 0.045	0.39LysTrp: 0.39 ± 0.016	0.579LysTyr: 0.579 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	13.775LeuAla: 13.775 ± 0.135	0.937LeuCys: 0.937 ± 0.028	6.14LeuAsp: 6.14 ± 0.075	4.674LeuGlu: 4.674 ± 0.065	3.68LeuPhe: 3.68 ± 0.06	7.938LeuGly: 7.938 ± 0.086	1.96LeuHis: 1.96 ± 0.043	5.259LeuIle: 5.259 ± 0.077	2.973LeuLys: 2.973 ± 0.048	9.744LeuLeu: 9.744 ± 0.12	2.321LeuMet: 2.321 ± 0.043	2.391LeuAsn: 2.391 ± 0.044	5.699LeuPro: 5.699 ± 0.063	2.452LeuGln: 2.452 ± 0.051	6.852LeuArg: 6.852 ± 0.067	6.258LeuSer: 6.258 ± 0.071	5.718LeuThr: 5.718 ± 0.075	6.859LeuVal: 6.859 ± 0.074	1.352LeuTrp: 1.352 ± 0.031	1.996LeuTyr: 1.996 ± 0.038	0.0LeuXaa: 0.0 ± 0.0
Met	3.692MetAla: 3.692 ± 0.054	0.159MetCys: 0.159 ± 0.011	1.31MetAsp: 1.31 ± 0.032	1.169MetGlu: 1.169 ± 0.027	0.762MetPhe: 0.762 ± 0.024	2.055MetGly: 2.055 ± 0.047	0.443MetHis: 0.443 ± 0.018	1.505MetIle: 1.505 ± 0.033	0.936MetLys: 0.936 ± 0.03	2.805MetLeu: 2.805 ± 0.051	0.772MetMet: 0.772 ± 0.023	0.717MetAsn: 0.717 ± 0.022	1.561MetPro: 1.561 ± 0.035	0.818MetGln: 0.818 ± 0.025	1.87MetArg: 1.87 ± 0.038	1.481MetSer: 1.481 ± 0.033	1.939MetThr: 1.939 ± 0.036	1.795MetVal: 1.795 ± 0.035	0.214MetTrp: 0.214 ± 0.012	0.274MetTyr: 0.274 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.285AsnAla: 3.285 ± 0.063	0.238AsnCys: 0.238 ± 0.014	1.462AsnAsp: 1.462 ± 0.035	1.095AsnGlu: 1.095 ± 0.027	0.903AsnPhe: 0.903 ± 0.028	2.462AsnGly: 2.462 ± 0.051	0.49AsnHis: 0.49 ± 0.017	1.423AsnIle: 1.423 ± 0.037	0.65AsnLys: 0.65 ± 0.023	2.401AsnLeu: 2.401 ± 0.051	0.631AsnMet: 0.631 ± 0.023	0.723AsnAsn: 0.723 ± 0.027	1.745AsnPro: 1.745 ± 0.038	0.762AsnGln: 0.762 ± 0.029	1.929AsnArg: 1.929 ± 0.039	1.365AsnSer: 1.365 ± 0.047	0.986AsnThr: 0.986 ± 0.033	1.818AsnVal: 1.818 ± 0.044	0.447AsnTrp: 0.447 ± 0.021	0.68AsnTyr: 0.68 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	6.852ProAla: 6.852 ± 0.08	0.362ProCys: 0.362 ± 0.017	4.132ProAsp: 4.132 ± 0.063	2.948ProGlu: 2.948 ± 0.044	1.899ProPhe: 1.899 ± 0.038	4.607ProGly: 4.607 ± 0.06	1.068ProHis: 1.068 ± 0.028	2.53ProIle: 2.53 ± 0.042	1.517ProLys: 1.517 ± 0.039	4.929ProLeu: 4.929 ± 0.061	1.376ProMet: 1.376 ± 0.033	1.235ProAsn: 1.235 ± 0.035	2.787ProPro: 2.787 ± 0.056	1.813ProGln: 1.813 ± 0.037	3.084ProArg: 3.084 ± 0.056	2.747ProSer: 2.747 ± 0.038	2.695ProThr: 2.695 ± 0.051	4.473ProVal: 4.473 ± 0.058	0.707ProTrp: 0.707 ± 0.025	1.093ProTyr: 1.093 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.569GlnAla: 4.569 ± 0.064	0.274GlnCys: 0.274 ± 0.015	1.641GlnAsp: 1.641 ± 0.031	1.39GlnGlu: 1.39 ± 0.034	1.13GlnPhe: 1.13 ± 0.029	2.701GlnGly: 2.701 ± 0.042	0.675GlnHis: 0.675 ± 0.023	1.878GlnIle: 1.878 ± 0.035	0.994GlnLys: 0.994 ± 0.028	3.143GlnLeu: 3.143 ± 0.052	0.931GlnMet: 0.931 ± 0.026	0.812GlnAsn: 0.812 ± 0.024	1.9GlnPro: 1.9 ± 0.042	1.279GlnGln: 1.279 ± 0.04	2.703GlnArg: 2.703 ± 0.053	1.944GlnSer: 1.944 ± 0.038	1.728GlnThr: 1.728 ± 0.038	2.365GlnVal: 2.365 ± 0.038	0.513GlnTrp: 0.513 ± 0.021	0.638GlnTyr: 0.638 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	8.928ArgAla: 8.928 ± 0.103	0.56ArgCys: 0.56 ± 0.019	4.38ArgAsp: 4.38 ± 0.059	3.757ArgGlu: 3.757 ± 0.063	2.988ArgPhe: 2.988 ± 0.055	4.929ArgGly: 4.929 ± 0.072	1.828ArgHis: 1.828 ± 0.038	4.289ArgIle: 4.289 ± 0.051	2.21ArgLys: 2.21 ± 0.042	8.052ArgLeu: 8.052 ± 0.102	1.948ArgMet: 1.948 ± 0.038	1.901ArgAsn: 1.901 ± 0.038	3.668ArgPro: 3.668 ± 0.049	2.748ArgGln: 2.748 ± 0.048	5.731ArgArg: 5.731 ± 0.095	3.71ArgSer: 3.71 ± 0.051	3.646ArgThr: 3.646 ± 0.049	4.604ArgVal: 4.604 ± 0.064	1.251ArgTrp: 1.251 ± 0.034	1.916ArgTyr: 1.916 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	6.834SerAla: 6.834 ± 0.078	0.447SerCys: 0.447 ± 0.02	3.233SerAsp: 3.233 ± 0.052	2.439SerGlu: 2.439 ± 0.046	2.197SerPhe: 2.197 ± 0.052	5.426SerGly: 5.426 ± 0.087	1.068SerHis: 1.068 ± 0.028	2.811SerIle: 2.811 ± 0.049	1.411SerLys: 1.411 ± 0.038	5.231SerLeu: 5.231 ± 0.066	1.293SerMet: 1.293 ± 0.032	1.404SerAsn: 1.404 ± 0.036	2.918SerPro: 2.918 ± 0.049	1.64SerGln: 1.64 ± 0.035	3.613SerArg: 3.613 ± 0.047	2.906SerSer: 2.906 ± 0.059	2.616SerThr: 2.616 ± 0.056	3.834SerVal: 3.834 ± 0.059	0.814SerTrp: 0.814 ± 0.027	1.418SerTyr: 1.418 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	6.267ThrAla: 6.267 ± 0.08	0.407ThrCys: 0.407 ± 0.021	2.97ThrAsp: 2.97 ± 0.062	2.179ThrGlu: 2.179 ± 0.043	1.69ThrPhe: 1.69 ± 0.039	5.389ThrGly: 5.389 ± 0.084	0.999ThrHis: 0.999 ± 0.029	3.117ThrIle: 3.117 ± 0.052	1.337ThrLys: 1.337 ± 0.034	5.912ThrLeu: 5.912 ± 0.084	1.294ThrMet: 1.294 ± 0.029	1.344ThrAsn: 1.344 ± 0.045	3.486ThrPro: 3.486 ± 0.06	1.625ThrGln: 1.625 ± 0.03	3.414ThrArg: 3.414 ± 0.053	2.746ThrSer: 2.746 ± 0.053	2.594ThrThr: 2.594 ± 0.056	3.999ThrVal: 3.999 ± 0.075	0.582ThrTrp: 0.582 ± 0.02	1.125ThrTyr: 1.125 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	9.473ValAla: 9.473 ± 0.09	0.507ValCys: 0.507 ± 0.022	4.357ValAsp: 4.357 ± 0.058	4.243ValGlu: 4.243 ± 0.06	1.891ValPhe: 1.891 ± 0.037	5.472ValGly: 5.472 ± 0.07	1.38ValHis: 1.38 ± 0.032	3.774ValIle: 3.774 ± 0.057	2.103ValLys: 2.103 ± 0.046	6.061ValLeu: 6.061 ± 0.068	1.752ValMet: 1.752 ± 0.036	1.895ValAsn: 1.895 ± 0.046	3.782ValPro: 3.782 ± 0.049	2.265ValGln: 2.265 ± 0.044	5.006ValArg: 5.006 ± 0.057	4.03ValSer: 4.03 ± 0.066	4.518ValThr: 4.518 ± 0.077	4.882ValVal: 4.882 ± 0.081	0.829ValTrp: 0.829 ± 0.026	1.392ValTyr: 1.392 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.426TrpAla: 1.426 ± 0.039	0.127TrpCys: 0.127 ± 0.011	0.783TrpAsp: 0.783 ± 0.023	0.607TrpGlu: 0.607 ± 0.021	0.532TrpPhe: 0.532 ± 0.019	0.937TrpGly: 0.937 ± 0.03	0.364TrpHis: 0.364 ± 0.016	0.715TrpIle: 0.715 ± 0.021	0.478TrpLys: 0.478 ± 0.019	1.782TrpLeu: 1.782 ± 0.043	0.39TrpMet: 0.39 ± 0.017	0.449TrpAsn: 0.449 ± 0.019	0.754TrpPro: 0.754 ± 0.024	0.612TrpGln: 0.612 ± 0.022	1.355TrpArg: 1.355 ± 0.03	0.952TrpSer: 0.952 ± 0.03	0.878TrpThr: 0.878 ± 0.034	0.836TrpVal: 0.836 ± 0.026	0.241TrpTrp: 0.241 ± 0.014	0.284TrpTyr: 0.284 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.689TyrAla: 2.689 ± 0.044	0.252TyrCys: 0.252 ± 0.013	1.596TyrAsp: 1.596 ± 0.035	1.057TyrGlu: 1.057 ± 0.028	0.852TyrPhe: 0.852 ± 0.025	2.153TyrGly: 2.153 ± 0.043	0.545TyrHis: 0.545 ± 0.021	0.895TyrIle: 0.895 ± 0.026	0.581TyrLys: 0.581 ± 0.022	2.184TyrLeu: 2.184 ± 0.039	0.491TyrMet: 0.491 ± 0.02	0.599TyrAsn: 0.599 ± 0.023	1.029TyrPro: 1.029 ± 0.026	0.74TyrGln: 0.74 ± 0.025	1.949TyrArg: 1.949 ± 0.035	1.204TyrSer: 1.204 ± 0.034	1.015TyrThr: 1.015 ± 0.033	1.608TyrVal: 1.608 ± 0.035	0.344TyrTrp: 0.344 ± 0.014	0.625TyrTyr: 0.625 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4670 proteins (1388006 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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