Sphingomonas sp. Ant20

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3084 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A6D127|A0A0A6D127_9SPHN Uncharacterized protein OS=Sphingomonas sp. Ant20 OX=104605 GN=NI18_11060 PE=4 SV=1
MM1 pKa = 7.42TYY3 pKa = 10.71VVTDD7 pKa = 3.09ACIRR11 pKa = 11.84CKK13 pKa = 11.04YY14 pKa = 7.9MDD16 pKa = 4.38CVEE19 pKa = 4.32VCPVDD24 pKa = 3.79CFYY27 pKa = 11.19EE28 pKa = 4.58GEE30 pKa = 4.18NMLVINPSEE39 pKa = 4.67CIDD42 pKa = 4.07CGVCEE47 pKa = 4.44PEE49 pKa = 4.82CPAEE53 pKa = 5.13AILPDD58 pKa = 3.95TEE60 pKa = 4.74SGLEE64 pKa = 3.64QWLEE68 pKa = 4.01LNTTFSAQWPNVTRR82 pKa = 11.84NDD84 pKa = 3.41NQTPPDD90 pKa = 3.95ADD92 pKa = 3.05AMKK95 pKa = 10.5GVEE98 pKa = 4.37GKK100 pKa = 9.86FDD102 pKa = 3.67KK103 pKa = 11.17YY104 pKa = 10.72FSPEE108 pKa = 3.84PGAGDD113 pKa = 3.34

Molecular weight: 12.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A6D4Z1|A0A0A6D4Z1_9SPHN PapD_N domain-containing protein OS=Sphingomonas sp. Ant20 OX=104605 GN=NI18_01455 PE=4 SV=1
MM1 pKa = 7.48TRR3 pKa = 11.84TCMDD7 pKa = 3.85CPTEE11 pKa = 4.0LGARR15 pKa = 11.84NLTGRR20 pKa = 11.84CKK22 pKa = 10.54SCATRR27 pKa = 11.84HH28 pKa = 5.34NNADD32 pKa = 3.18PVAAEE37 pKa = 4.47KK38 pKa = 10.43RR39 pKa = 11.84RR40 pKa = 11.84QGIARR45 pKa = 11.84RR46 pKa = 11.84FADD49 pKa = 3.48PEE51 pKa = 3.74YY52 pKa = 10.33RR53 pKa = 11.84AAHH56 pKa = 6.88AARR59 pKa = 11.84CASMNRR65 pKa = 11.84HH66 pKa = 6.09ISPEE70 pKa = 3.66VRR72 pKa = 11.84EE73 pKa = 3.95QRR75 pKa = 11.84RR76 pKa = 11.84QHH78 pKa = 4.44GHH80 pKa = 6.34RR81 pKa = 11.84MTATLRR87 pKa = 11.84AANDD91 pKa = 4.49KK92 pKa = 8.98ITKK95 pKa = 7.51DD96 pKa = 3.23QRR98 pKa = 11.84MAAGAARR105 pKa = 11.84SATVLAWCPPEE116 pKa = 3.75WRR118 pKa = 11.84EE119 pKa = 4.56KK120 pKa = 10.9YY121 pKa = 10.04RR122 pKa = 11.84DD123 pKa = 3.54LKK125 pKa = 11.03RR126 pKa = 11.84GGRR129 pKa = 11.84PAADD133 pKa = 3.63AKK135 pKa = 10.98RR136 pKa = 11.84LVLDD140 pKa = 5.11LIAGKK145 pKa = 9.57PMPTPYY151 pKa = 10.67AKK153 pKa = 9.84QRR155 pKa = 11.84AALNWCPPARR165 pKa = 11.84RR166 pKa = 11.84AEE168 pKa = 4.22YY169 pKa = 10.56ASARR173 pKa = 11.84AALGAAEE180 pKa = 4.18ARR182 pKa = 11.84RR183 pKa = 11.84IIEE186 pKa = 4.1ADD188 pKa = 3.05MTPFEE193 pKa = 4.13RR194 pKa = 11.84QMARR198 pKa = 11.84IAAGAEE204 pKa = 3.91LVAAPDD210 pKa = 3.66TRR212 pKa = 11.84TGGPAYY218 pKa = 8.94TLGGISSGMLL228 pKa = 3.03

Molecular weight: 24.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3084

0

3084

807437

29

1393

261.8

28.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.719 ± 0.075

0.775 ± 0.013

6.162 ± 0.036

4.932 ± 0.04

3.411 ± 0.028

8.78 ± 0.046

1.952 ± 0.021

5.05 ± 0.034

2.892 ± 0.038

9.634 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.023

2.375 ± 0.034

5.178 ± 0.034

3.026 ± 0.025

7.632 ± 0.052

5.165 ± 0.038

5.884 ± 0.045

7.448 ± 0.034

1.38 ± 0.02

2.136 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski