Amino acid dipepetide frequency for Spizellomyces punctatus (strain DAOM BR117)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.166AlaAla: 8.166 ± 0.079	1.038AlaCys: 1.038 ± 0.019	4.268AlaAsp: 4.268 ± 0.034	5.245AlaGlu: 5.245 ± 0.05	2.93AlaPhe: 2.93 ± 0.026	5.191AlaGly: 5.191 ± 0.039	1.795AlaHis: 1.795 ± 0.021	3.979AlaIle: 3.979 ± 0.029	4.418AlaLys: 4.418 ± 0.044	7.66AlaLeu: 7.66 ± 0.046	1.897AlaMet: 1.897 ± 0.023	2.914AlaAsn: 2.914 ± 0.03	4.285AlaPro: 4.285 ± 0.046	3.207AlaGln: 3.207 ± 0.028	4.615AlaArg: 4.615 ± 0.032	6.664AlaSer: 6.664 ± 0.052	5.184AlaThr: 5.184 ± 0.041	5.61AlaVal: 5.61 ± 0.045	0.923AlaTrp: 0.923 ± 0.015	2.101AlaTyr: 2.101 ± 0.025	0.0AlaXaa: 0.0 ± 0.0
Cys	0.927CysAla: 0.927 ± 0.017	0.246CysCys: 0.246 ± 0.008	0.633CysAsp: 0.633 ± 0.014	0.669CysGlu: 0.669 ± 0.013	0.536CysPhe: 0.536 ± 0.012	0.945CysGly: 0.945 ± 0.018	0.334CysHis: 0.334 ± 0.009	0.721CysIle: 0.721 ± 0.013	0.603CysLys: 0.603 ± 0.012	1.295CysLeu: 1.295 ± 0.02	0.271CysMet: 0.271 ± 0.008	0.442CysAsn: 0.442 ± 0.012	0.705CysPro: 0.705 ± 0.017	0.462CysGln: 0.462 ± 0.011	0.814CysArg: 0.814 ± 0.015	0.903CysSer: 0.903 ± 0.017	0.774CysThr: 0.774 ± 0.016	0.856CysVal: 0.856 ± 0.012	0.169CysTrp: 0.169 ± 0.006	0.351CysTyr: 0.351 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	4.809AspAla: 4.809 ± 0.039	0.6AspCys: 0.6 ± 0.012	4.066AspAsp: 4.066 ± 0.044	4.478AspGlu: 4.478 ± 0.038	2.091AspPhe: 2.091 ± 0.023	3.832AspGly: 3.832 ± 0.032	1.189AspHis: 1.189 ± 0.017	3.171AspIle: 3.171 ± 0.026	2.533AspLys: 2.533 ± 0.026	5.214AspLeu: 5.214 ± 0.039	1.391AspMet: 1.391 ± 0.019	1.807AspAsn: 1.807 ± 0.021	3.138AspPro: 3.138 ± 0.026	1.846AspGln: 1.846 ± 0.018	3.161AspArg: 3.161 ± 0.031	3.697AspSer: 3.697 ± 0.031	3.167AspThr: 3.167 ± 0.027	4.278AspVal: 4.278 ± 0.033	0.781AspTrp: 0.781 ± 0.014	1.51AspTyr: 1.51 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	5.248GluAla: 5.248 ± 0.05	0.723GluCys: 0.723 ± 0.014	4.26GluAsp: 4.26 ± 0.034	6.124GluGlu: 6.124 ± 0.071	2.006GluPhe: 2.006 ± 0.02	3.923GluGly: 3.923 ± 0.032	1.485GluHis: 1.485 ± 0.018	3.208GluIle: 3.208 ± 0.026	4.004GluLys: 4.004 ± 0.046	5.684GluLeu: 5.684 ± 0.051	1.56GluMet: 1.56 ± 0.021	2.479GluAsn: 2.479 ± 0.022	2.598GluPro: 2.598 ± 0.032	2.802GluGln: 2.802 ± 0.03	4.537GluArg: 4.537 ± 0.049	4.31GluSer: 4.31 ± 0.035	3.691GluThr: 3.691 ± 0.033	3.898GluVal: 3.898 ± 0.026	0.842GluTrp: 0.842 ± 0.013	1.694GluTyr: 1.694 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	2.88PheAla: 2.88 ± 0.026	0.573PheCys: 0.573 ± 0.013	2.146PheAsp: 2.146 ± 0.021	2.148PheGlu: 2.148 ± 0.022	1.453PhePhe: 1.453 ± 0.023	2.653PheGly: 2.653 ± 0.028	0.896PheHis: 0.896 ± 0.016	1.735PheIle: 1.735 ± 0.021	1.602PheLys: 1.602 ± 0.019	3.402PheLeu: 3.402 ± 0.029	0.764PheMet: 0.764 ± 0.012	1.302PheAsn: 1.302 ± 0.019	1.823PhePro: 1.823 ± 0.018	1.29PheGln: 1.29 ± 0.018	1.965PheArg: 1.965 ± 0.018	2.654PheSer: 2.654 ± 0.025	2.041PheThr: 2.041 ± 0.023	2.459PheVal: 2.459 ± 0.03	0.517PheTrp: 0.517 ± 0.01	1.052PheTyr: 1.052 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	4.753GlyAla: 4.753 ± 0.038	0.82GlyCys: 0.82 ± 0.014	3.504GlyAsp: 3.504 ± 0.03	3.632GlyGlu: 3.632 ± 0.028	2.458GlyPhe: 2.458 ± 0.027	5.09GlyGly: 5.09 ± 0.055	1.597GlyHis: 1.597 ± 0.019	3.285GlyIle: 3.285 ± 0.027	3.668GlyLys: 3.668 ± 0.037	5.418GlyLeu: 5.418 ± 0.045	1.558GlyMet: 1.558 ± 0.023	2.412GlyAsn: 2.412 ± 0.03	2.822GlyPro: 2.822 ± 0.032	2.377GlyGln: 2.377 ± 0.024	4.081GlyArg: 4.081 ± 0.034	5.201GlySer: 5.201 ± 0.043	4.034GlyThr: 4.034 ± 0.036	4.299GlyVal: 4.299 ± 0.034	0.97GlyTrp: 0.97 ± 0.015	1.913GlyTyr: 1.913 ± 0.026	0.0GlyXaa: 0.0 ± 0.0
His	1.852HisAla: 1.852 ± 0.019	0.333HisCys: 0.333 ± 0.009	1.208HisAsp: 1.208 ± 0.017	1.342HisGlu: 1.342 ± 0.019	0.941HisPhe: 0.941 ± 0.015	1.546HisGly: 1.546 ± 0.019	0.875HisHis: 0.875 ± 0.019	1.316HisIle: 1.316 ± 0.02	1.016HisLys: 1.016 ± 0.015	2.456HisLeu: 2.456 ± 0.027	0.492HisMet: 0.492 ± 0.012	0.765HisAsn: 0.765 ± 0.013	1.764HisPro: 1.764 ± 0.025	1.01HisGln: 1.01 ± 0.019	1.543HisArg: 1.543 ± 0.021	1.788HisSer: 1.788 ± 0.022	1.388HisThr: 1.388 ± 0.018	1.652HisVal: 1.652 ± 0.019	0.293HisTrp: 0.293 ± 0.007	0.698HisTyr: 0.698 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.073IleAla: 4.073 ± 0.035	0.74IleCys: 0.74 ± 0.013	2.837IleAsp: 2.837 ± 0.027	2.912IleGlu: 2.912 ± 0.029	1.807IlePhe: 1.807 ± 0.024	3.061IleGly: 3.061 ± 0.028	1.217IleHis: 1.217 ± 0.015	2.429IleIle: 2.429 ± 0.026	2.449IleLys: 2.449 ± 0.028	4.801IleLeu: 4.801 ± 0.039	1.082IleMet: 1.082 ± 0.015	1.751IleAsn: 1.751 ± 0.02	3.216IlePro: 3.216 ± 0.032	1.984IleGln: 1.984 ± 0.023	3.002IleArg: 3.002 ± 0.031	3.751IleSer: 3.751 ± 0.028	2.832IleThr: 2.832 ± 0.024	3.495IleVal: 3.495 ± 0.027	0.639IleTrp: 0.639 ± 0.013	1.331IleTyr: 1.331 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	4.358LysAla: 4.358 ± 0.045	0.603LysCys: 0.603 ± 0.012	3.005LysAsp: 3.005 ± 0.032	4.012LysGlu: 4.012 ± 0.041	1.53LysPhe: 1.53 ± 0.021	3.205LysGly: 3.205 ± 0.029	1.24LysHis: 1.24 ± 0.018	2.43LysIle: 2.43 ± 0.024	3.76LysLys: 3.76 ± 0.047	4.536LysLeu: 4.536 ± 0.037	1.164LysMet: 1.164 ± 0.017	1.866LysAsn: 1.866 ± 0.024	2.667LysPro: 2.667 ± 0.029	2.162LysGln: 2.162 ± 0.028	4.019LysArg: 4.019 ± 0.036	3.682LysSer: 3.682 ± 0.035	3.059LysThr: 3.059 ± 0.032	3.272LysVal: 3.272 ± 0.029	0.669LysTrp: 0.669 ± 0.011	1.466LysTyr: 1.466 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	7.568LeuAla: 7.568 ± 0.046	1.303LeuCys: 1.303 ± 0.018	5.276LeuAsp: 5.276 ± 0.035	6.076LeuGlu: 6.076 ± 0.054	3.244LeuPhe: 3.244 ± 0.029	5.4LeuGly: 5.4 ± 0.04	2.373LeuHis: 2.373 ± 0.026	3.958LeuIle: 3.958 ± 0.036	4.911LeuLys: 4.911 ± 0.045	8.797LeuLeu: 8.797 ± 0.059	1.885LeuMet: 1.885 ± 0.022	3.251LeuAsn: 3.251 ± 0.031	5.393LeuPro: 5.393 ± 0.04	4.169LeuGln: 4.169 ± 0.038	6.104LeuArg: 6.104 ± 0.045	7.331LeuSer: 7.331 ± 0.043	5.121LeuThr: 5.121 ± 0.034	5.86LeuVal: 5.86 ± 0.041	1.065LeuTrp: 1.065 ± 0.017	2.482LeuTyr: 2.482 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	1.938MetAla: 1.938 ± 0.022	0.265MetCys: 0.265 ± 0.008	1.419MetAsp: 1.419 ± 0.017	1.575MetGlu: 1.575 ± 0.019	0.704MetPhe: 0.704 ± 0.012	1.399MetGly: 1.399 ± 0.017	0.521MetHis: 0.521 ± 0.01	0.994MetIle: 0.994 ± 0.014	1.092MetLys: 1.092 ± 0.016	1.948MetLeu: 1.948 ± 0.02	0.587MetMet: 0.587 ± 0.012	0.851MetAsn: 0.851 ± 0.015	1.141MetPro: 1.141 ± 0.016	0.927MetGln: 0.927 ± 0.016	1.361MetArg: 1.361 ± 0.019	1.871MetSer: 1.871 ± 0.018	1.354MetThr: 1.354 ± 0.016	1.431MetVal: 1.431 ± 0.018	0.266MetTrp: 0.266 ± 0.007	0.597MetTyr: 0.597 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	3.121AsnAla: 3.121 ± 0.031	0.434AsnCys: 0.434 ± 0.011	2.045AsnAsp: 2.045 ± 0.022	2.178AsnGlu: 2.178 ± 0.027	1.234AsnPhe: 1.234 ± 0.018	2.748AsnGly: 2.748 ± 0.033	0.773AsnHis: 0.773 ± 0.012	2.011AsnIle: 2.011 ± 0.021	1.662AsnLys: 1.662 ± 0.022	3.461AsnLeu: 3.461 ± 0.034	0.859AsnMet: 0.859 ± 0.014	1.357AsnAsn: 1.357 ± 0.036	2.245AsnPro: 2.245 ± 0.023	1.296AsnGln: 1.296 ± 0.017	2.058AsnArg: 2.058 ± 0.02	2.418AsnSer: 2.418 ± 0.024	2.032AsnThr: 2.032 ± 0.022	2.766AsnVal: 2.766 ± 0.025	0.473AsnTrp: 0.473 ± 0.009	0.947AsnTyr: 0.947 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	4.753ProAla: 4.753 ± 0.04	0.546ProCys: 0.546 ± 0.013	2.979ProAsp: 2.979 ± 0.024	3.387ProGlu: 3.387 ± 0.033	1.966ProPhe: 1.966 ± 0.021	3.206ProGly: 3.206 ± 0.037	1.395ProHis: 1.395 ± 0.021	2.712ProIle: 2.712 ± 0.028	2.659ProLys: 2.659 ± 0.026	4.755ProLeu: 4.755 ± 0.036	1.0ProMet: 1.0 ± 0.017	2.138ProAsn: 2.138 ± 0.025	5.341ProPro: 5.341 ± 0.075	2.25ProGln: 2.25 ± 0.025	3.058ProArg: 3.058 ± 0.03	5.868ProSer: 5.868 ± 0.06	4.289ProThr: 4.289 ± 0.044	3.876ProVal: 3.876 ± 0.037	0.559ProTrp: 0.559 ± 0.011	1.463ProTyr: 1.463 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	3.169GlnAla: 3.169 ± 0.031	0.461GlnCys: 0.461 ± 0.011	1.964GlnAsp: 1.964 ± 0.02	2.62GlnGlu: 2.62 ± 0.03	1.251GlnPhe: 1.251 ± 0.016	2.118GlnGly: 2.118 ± 0.023	1.085GlnHis: 1.085 ± 0.018	1.938GlnIle: 1.938 ± 0.023	2.263GlnLys: 2.263 ± 0.024	3.724GlnLeu: 3.724 ± 0.036	0.952GlnMet: 0.952 ± 0.015	1.546GlnAsn: 1.546 ± 0.018	2.304GlnPro: 2.304 ± 0.034	2.651GlnGln: 2.651 ± 0.061	2.785GlnArg: 2.785 ± 0.027	3.106GlnSer: 3.106 ± 0.028	2.459GlnThr: 2.459 ± 0.024	2.365GlnVal: 2.365 ± 0.023	0.494GlnTrp: 0.494 ± 0.012	1.118GlnTyr: 1.118 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	4.526ArgAla: 4.526 ± 0.036	0.764ArgCys: 0.764 ± 0.016	3.341ArgAsp: 3.341 ± 0.033	4.035ArgGlu: 4.035 ± 0.04	2.189ArgPhe: 2.189 ± 0.023	3.534ArgGly: 3.534 ± 0.036	1.576ArgHis: 1.576 ± 0.021	3.24ArgIle: 3.24 ± 0.027	4.006ArgLys: 4.006 ± 0.035	5.689ArgLeu: 5.689 ± 0.04	1.483ArgMet: 1.483 ± 0.019	2.37ArgAsn: 2.37 ± 0.023	3.361ArgPro: 3.361 ± 0.034	2.639ArgGln: 2.639 ± 0.027	5.333ArgArg: 5.333 ± 0.045	4.905ArgSer: 4.905 ± 0.046	3.726ArgThr: 3.726 ± 0.027	3.708ArgVal: 3.708 ± 0.034	0.805ArgTrp: 0.805 ± 0.014	1.709ArgTyr: 1.709 ± 0.021	0.0ArgXaa: 0.0 ± 0.0
Ser	6.36SerAla: 6.36 ± 0.046	0.856SerCys: 0.856 ± 0.016	4.175SerAsp: 4.175 ± 0.038	4.232SerGlu: 4.232 ± 0.037	2.75SerPhe: 2.75 ± 0.024	5.171SerGly: 5.171 ± 0.046	1.906SerHis: 1.906 ± 0.02	3.871SerIle: 3.871 ± 0.028	3.754SerLys: 3.754 ± 0.032	7.11SerLeu: 7.11 ± 0.042	1.663SerMet: 1.663 ± 0.021	2.791SerAsn: 2.791 ± 0.027	5.284SerPro: 5.284 ± 0.057	3.021SerGln: 3.021 ± 0.028	4.733SerArg: 4.733 ± 0.041	8.071SerSer: 8.071 ± 0.076	5.631SerThr: 5.631 ± 0.04	5.038SerVal: 5.038 ± 0.033	0.851SerTrp: 0.851 ± 0.014	1.826SerTyr: 1.826 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	5.139ThrAla: 5.139 ± 0.039	0.812ThrCys: 0.812 ± 0.017	3.051ThrAsp: 3.051 ± 0.024	3.333ThrGlu: 3.333 ± 0.03	2.288ThrPhe: 2.288 ± 0.022	4.11ThrGly: 4.11 ± 0.036	1.403ThrHis: 1.403 ± 0.019	3.172ThrIle: 3.172 ± 0.028	2.806ThrLys: 2.806 ± 0.026	5.807ThrLeu: 5.807 ± 0.04	1.228ThrMet: 1.228 ± 0.015	2.074ThrAsn: 2.074 ± 0.023	4.362ThrPro: 4.362 ± 0.041	2.1ThrGln: 2.1 ± 0.024	3.305ThrArg: 3.305 ± 0.028	5.458ThrSer: 5.458 ± 0.039	4.455ThrThr: 4.455 ± 0.089	4.252ThrVal: 4.252 ± 0.039	0.732ThrTrp: 0.732 ± 0.013	1.566ThrTyr: 1.566 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	5.501ValAla: 5.501 ± 0.039	0.907ValCys: 0.907 ± 0.015	4.178ValAsp: 4.178 ± 0.031	4.667ValGlu: 4.667 ± 0.037	2.379ValPhe: 2.379 ± 0.026	4.168ValGly: 4.168 ± 0.037	1.62ValHis: 1.62 ± 0.018	3.143ValIle: 3.143 ± 0.028	3.498ValLys: 3.498 ± 0.032	6.077ValLeu: 6.077 ± 0.04	1.432ValMet: 1.432 ± 0.02	2.394ValAsn: 2.394 ± 0.022	3.912ValPro: 3.912 ± 0.035	2.678ValGln: 2.678 ± 0.027	3.904ValArg: 3.904 ± 0.034	4.733ValSer: 4.733 ± 0.03	3.88ValThr: 3.88 ± 0.032	4.838ValVal: 4.838 ± 0.037	0.822ValTrp: 0.822 ± 0.015	1.749ValTyr: 1.749 ± 0.021	0.0ValXaa: 0.0 ± 0.0
Trp	0.892TrpAla: 0.892 ± 0.015	0.189TrpCys: 0.189 ± 0.007	0.799TrpAsp: 0.799 ± 0.016	0.787TrpGlu: 0.787 ± 0.015	0.498TrpPhe: 0.498 ± 0.011	0.727TrpGly: 0.727 ± 0.012	0.284TrpHis: 0.284 ± 0.008	0.674TrpIle: 0.674 ± 0.013	0.797TrpLys: 0.797 ± 0.015	1.072TrpLeu: 1.072 ± 0.016	0.354TrpMet: 0.354 ± 0.009	0.584TrpAsn: 0.584 ± 0.012	0.449TrpPro: 0.449 ± 0.01	0.445TrpGln: 0.445 ± 0.01	0.863TrpArg: 0.863 ± 0.014	0.903TrpSer: 0.903 ± 0.013	0.794TrpThr: 0.794 ± 0.016	0.78TrpVal: 0.78 ± 0.012	0.243TrpTrp: 0.243 ± 0.007	0.349TrpTyr: 0.349 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.057TyrAla: 2.057 ± 0.023	0.444TyrCys: 0.444 ± 0.011	1.605TyrAsp: 1.605 ± 0.022	1.626TyrGlu: 1.626 ± 0.022	1.099TyrPhe: 1.099 ± 0.019	1.866TyrGly: 1.866 ± 0.025	0.714TyrHis: 0.714 ± 0.013	1.424TyrIle: 1.424 ± 0.021	1.203TyrLys: 1.203 ± 0.019	2.663TyrLeu: 2.663 ± 0.029	0.617TyrMet: 0.617 ± 0.011	1.025TyrAsn: 1.025 ± 0.015	1.388TyrPro: 1.388 ± 0.022	1.058TyrGln: 1.058 ± 0.018	1.7TyrArg: 1.7 ± 0.018	1.781TyrSer: 1.781 ± 0.02	1.54TyrThr: 1.54 ± 0.022	1.744TyrVal: 1.744 ± 0.02	0.368TyrTrp: 0.368 ± 0.01	0.91TyrTyr: 0.91 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9267 proteins (4819746 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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