Mastomys coucha papillomavirus 2
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q06RH5|Q06RH5_9PAPI Protein E6 OS=Mastomys coucha papillomavirus 2 OX=392505 GN=E6 PE=3 SV=1
MM1 pKa = 7.59 LGPVPTLRR9 pKa = 11.84 DD10 pKa = 3.26 IEE12 pKa = 4.62 LVDD15 pKa = 4.39 LEE17 pKa = 4.38 VLAAHH22 pKa = 6.61 VPPADD27 pKa = 3.65 QPDD30 pKa = 3.93 VGSSSLSPDD39 pKa = 3.14 SLGEE43 pKa = 4.03 EE44 pKa = 4.48 VEE46 pKa = 4.99 LEE48 pKa = 3.84 VDD50 pKa = 4.38 PYY52 pKa = 11.03 RR53 pKa = 11.84 VATTCYY59 pKa = 10.5 SCDD62 pKa = 3.16 TVLRR66 pKa = 11.84 FIVVAGCDD74 pKa = 3.38 SLKK77 pKa = 10.81 LFEE80 pKa = 4.95 EE81 pKa = 4.83 LLTEE85 pKa = 5.72 DD86 pKa = 5.64 FDD88 pKa = 4.95 FLCPGCVKK96 pKa = 10.65 DD97 pKa = 3.92 RR98 pKa = 11.84 LLRR101 pKa = 11.84 KK102 pKa = 9.66 RR103 pKa = 11.84 NNGRR107 pKa = 11.84 RR108 pKa = 11.84 KK109 pKa = 9.84
Molecular weight: 12.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.515
IPC2_protein 4.507
IPC_protein 4.406
Toseland 4.228
ProMoST 4.533
Dawson 4.368
Bjellqvist 4.52
Wikipedia 4.266
Rodwell 4.24
Grimsley 4.139
Solomon 4.355
Lehninger 4.317
Nozaki 4.482
DTASelect 4.673
Thurlkill 4.253
EMBOSS 4.279
Sillero 4.52
Patrickios 3.808
IPC_peptide 4.368
IPC2_peptide 4.507
IPC2.peptide.svr19 4.475
Protein with the highest isoelectric point:
>tr|Q06RH3|Q06RH3_9PAPI Replication protein E1 OS=Mastomys coucha papillomavirus 2 OX=392505 GN=E1 PE=3 SV=1
MM1 pKa = 7.48 NSLEE5 pKa = 3.98 ARR7 pKa = 11.84 FDD9 pKa = 3.64 AVQEE13 pKa = 4.32 QILSLYY19 pKa = 10.1 EE20 pKa = 4.07 KK21 pKa = 10.71 GSTDD25 pKa = 3.52 LADD28 pKa = 3.39 HH29 pKa = 5.68 VKK31 pKa = 9.41 YY32 pKa = 9.99 WEE34 pKa = 4.7 LIRR37 pKa = 11.84 AEE39 pKa = 4.46 GALEE43 pKa = 3.88 YY44 pKa = 10.39 CARR47 pKa = 11.84 QSGRR51 pKa = 11.84 NKK53 pKa = 10.44 LGLHH57 pKa = 5.88 SVPSSVGAEE66 pKa = 4.11 SKK68 pKa = 10.61 AKK70 pKa = 10.2 KK71 pKa = 9.46 AIHH74 pKa = 5.41 MQMVLSSLQDD84 pKa = 3.13 SSFGSEE90 pKa = 4.01 PWTMADD96 pKa = 3.48 TSLEE100 pKa = 4.13 MYY102 pKa = 9.7 EE103 pKa = 4.24 RR104 pKa = 11.84 TSPEE108 pKa = 3.58 YY109 pKa = 9.76 TFKK112 pKa = 11.12 KK113 pKa = 9.88 KK114 pKa = 10.61 GSEE117 pKa = 4.11 AEE119 pKa = 4.25 VTFGSDD125 pKa = 3.12 PDD127 pKa = 3.8 NSVSYY132 pKa = 8.33 MVWGCVYY139 pKa = 9.85 TQDD142 pKa = 5.73 EE143 pKa = 4.37 DD144 pKa = 4.6 GQWHH148 pKa = 5.74 KK149 pKa = 10.81 HH150 pKa = 4.5 MSVVDD155 pKa = 3.84 YY156 pKa = 11.22 YY157 pKa = 10.96 GVYY160 pKa = 9.96 YY161 pKa = 10.45 VDD163 pKa = 3.43 HH164 pKa = 6.65 SGVRR168 pKa = 11.84 VYY170 pKa = 9.92 YY171 pKa = 10.29 HH172 pKa = 6.66 QFDD175 pKa = 4.15 EE176 pKa = 4.74 DD177 pKa = 4.14 SKK179 pKa = 10.16 TFGGDD184 pKa = 2.79 FWTVRR189 pKa = 11.84 YY190 pKa = 9.62 KK191 pKa = 11.04 LQTFTSSPDD200 pKa = 3.09 STTKK204 pKa = 10.65 EE205 pKa = 3.96 NEE207 pKa = 3.76 QGLDD211 pKa = 3.67 EE212 pKa = 4.9 PCHH215 pKa = 5.69 KK216 pKa = 10.12 RR217 pKa = 11.84 RR218 pKa = 11.84 RR219 pKa = 11.84 SSPCSTEE226 pKa = 4.05 TIRR229 pKa = 11.84 TPNRR233 pKa = 11.84 RR234 pKa = 11.84 ARR236 pKa = 11.84 PSEE239 pKa = 3.92 RR240 pKa = 11.84 PQPTSGRR247 pKa = 11.84 RR248 pKa = 11.84 GRR250 pKa = 11.84 LGQGEE255 pKa = 4.33 RR256 pKa = 11.84 TPEE259 pKa = 3.87 GTRR262 pKa = 11.84 PSPEE266 pKa = 3.94 EE267 pKa = 3.3 IGRR270 pKa = 11.84 RR271 pKa = 11.84 PEE273 pKa = 3.87 GSEE276 pKa = 3.83 RR277 pKa = 11.84 SCQRR281 pKa = 11.84 RR282 pKa = 11.84 VPNPVHH288 pKa = 7.01 CDD290 pKa = 3.36 PPVILLKK297 pKa = 11.05 GPTNTLKK304 pKa = 10.54 CWRR307 pKa = 11.84 NRR309 pKa = 11.84 LRR311 pKa = 11.84 RR312 pKa = 11.84 RR313 pKa = 11.84 HH314 pKa = 5.6 FRR316 pKa = 11.84 PFIKK320 pKa = 10.15 ISTAFSWVEE329 pKa = 3.74 DD330 pKa = 3.9 RR331 pKa = 11.84 DD332 pKa = 4.14 SGGVTNMLVAFLDD345 pKa = 3.71 SQQRR349 pKa = 11.84 DD350 pKa = 3.69 VFLKK354 pKa = 9.34 TVPIPKK360 pKa = 9.92 GSVYY364 pKa = 10.98 YY365 pKa = 10.48 KK366 pKa = 11.24 GNFEE370 pKa = 4.24 GLL372 pKa = 3.46
Molecular weight: 42.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.277
IPC2_protein 7.644
IPC_protein 7.556
Toseland 7.263
ProMoST 8.068
Dawson 8.229
Bjellqvist 8.551
Wikipedia 8.17
Rodwell 8.244
Grimsley 7.307
Solomon 8.331
Lehninger 8.331
Nozaki 8.697
DTASelect 8.317
Thurlkill 8.361
EMBOSS 8.448
Sillero 8.668
Patrickios 4.418
IPC_peptide 8.317
IPC2_peptide 7.556
IPC2.peptide.svr19 7.645
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2306
109
614
384.3
42.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.637 ± 0.849
2.298 ± 0.576
6.548 ± 0.323
6.071 ± 0.588
4.944 ± 0.345
7.329 ± 1.077
1.951 ± 0.249
3.903 ± 0.853
4.337 ± 0.875
8.066 ± 0.808
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.255 ± 0.417
4.163 ± 0.649
6.288 ± 0.96
3.99 ± 0.483
6.852 ± 0.437
7.502 ± 0.661
6.808 ± 0.4
7.112 ± 0.772
1.171 ± 0.311
2.775 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here