Acinetobacter phage YMC13/03/R2096
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 162 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0RMY3|A0A0A0RMY3_9CAUD Uncharacterized protein OS=Acinetobacter phage YMC13/03/R2096 OX=1560342 GN=BPABA577_01510 PE=4 SV=1
MM1 pKa = 7.03 NTFEE5 pKa = 4.52 YY6 pKa = 10.11 IKK8 pKa = 10.66 SAVASEE14 pKa = 5.02 IYY16 pKa = 9.86 QLPSISFDD24 pKa = 3.26 YY25 pKa = 11.07 HH26 pKa = 6.75 QDD28 pKa = 3.46 NEE30 pKa = 4.4 QALLDD35 pKa = 4.51 HH36 pKa = 6.93 IEE38 pKa = 4.23 VNGYY42 pKa = 7.69 CQPSEE47 pKa = 3.82 IGTTAEE53 pKa = 4.37 AYY55 pKa = 10.55 NIVWSDD61 pKa = 3.12 LARR64 pKa = 11.84 NTDD67 pKa = 3.24 YY68 pKa = 11.6 EE69 pKa = 4.26 MPDD72 pKa = 3.43 FSLCKK77 pKa = 10.23 SSLEE81 pKa = 4.29 CIEE84 pKa = 4.52 VEE86 pKa = 4.26 GQAILSGAYY95 pKa = 9.89 YY96 pKa = 10.26 EE97 pKa = 4.23 ATQEE101 pKa = 3.99 IVKK104 pKa = 9.87 EE105 pKa = 3.82 IASAIDD111 pKa = 3.07 EE112 pKa = 4.42 CFSYY116 pKa = 11.26 DD117 pKa = 3.88 FGNLEE122 pKa = 4.17 LTEE125 pKa = 4.34 MYY127 pKa = 10.38 LGEE130 pKa = 4.86 SGLGHH135 pKa = 6.87 IPHH138 pKa = 7.6 DD139 pKa = 4.21 YY140 pKa = 10.8 EE141 pKa = 5.53 IDD143 pKa = 3.56 IADD146 pKa = 3.46 SSMLVWVKK154 pKa = 11.03 SKK156 pKa = 10.09 MIQITVSDD164 pKa = 3.72 VTLYY168 pKa = 11.21 GILEE172 pKa = 4.13 QVEE175 pKa = 4.22 NN176 pKa = 4.15
Molecular weight: 19.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.912
IPC2_protein 3.897
IPC_protein 3.846
Toseland 3.668
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.694
Rodwell 3.681
Grimsley 3.579
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.062
Thurlkill 3.694
EMBOSS 3.706
Sillero 3.961
Patrickios 0.083
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.86
Protein with the highest isoelectric point:
>tr|A0A0A0RMI8|A0A0A0RMI8_9CAUD Phosphoprotein phosphatase OS=Acinetobacter phage YMC13/03/R2096 OX=1560342 GN=BPABA577_00010 PE=4 SV=1
MM1 pKa = 7.69 PKK3 pKa = 9.9 INQKK7 pKa = 10.12 GFALRR12 pKa = 11.84 GFMCGVLALPINTPCHH28 pKa = 4.99 VWLFPHH34 pKa = 7.62 RR35 pKa = 11.84 IAQVV39 pKa = 2.86
Molecular weight: 4.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.212
IPC2_protein 9.18
IPC_protein 9.385
Toseland 10.423
ProMoST 9.853
Dawson 10.467
Bjellqvist 10.101
Wikipedia 10.526
Rodwell 11.023
Grimsley 10.452
Solomon 10.57
Lehninger 10.584
Nozaki 10.511
DTASelect 10.043
Thurlkill 10.394
EMBOSS 10.789
Sillero 10.423
Patrickios 11.023
IPC_peptide 10.584
IPC2_peptide 9.531
IPC2.peptide.svr19 8.069
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
162
0
162
27861
38
923
172.0
19.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.682 ± 0.216
1.206 ± 0.094
6.335 ± 0.159
7.161 ± 0.254
4.278 ± 0.15
6.909 ± 0.162
1.892 ± 0.111
6.457 ± 0.163
7.667 ± 0.246
8.255 ± 0.191
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.724 ± 0.087
4.914 ± 0.224
3.058 ± 0.108
3.679 ± 0.113
4.343 ± 0.136
6.525 ± 0.232
5.714 ± 0.236
7.462 ± 0.151
1.403 ± 0.08
4.336 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here