Sphingomonas changbaiensis NBRC 104936

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; Sphingomonas changbaiensis

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3151 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E9MNF1|A0A0E9MNF1_9SPHN TPR_REGION domain-containing protein OS=Sphingomonas changbaiensis NBRC 104936 OX=1219043 GN=SCH01S_17_00140 PE=4 SV=1
MM1 pKa = 7.63TIFNVSNSTQLQSALGSATGGDD23 pKa = 4.23TIVLADD29 pKa = 3.7GDD31 pKa = 4.16YY32 pKa = 11.57GKK34 pKa = 11.26VNVYY38 pKa = 8.18TRR40 pKa = 11.84NYY42 pKa = 9.4ASTVTIEE49 pKa = 3.62AATPGGAHH57 pKa = 7.37LDD59 pKa = 3.69GLFVANATNISFAGLDD75 pKa = 3.24VGRR78 pKa = 11.84ALGATEE84 pKa = 4.3PDD86 pKa = 3.7YY87 pKa = 10.91TQLSSVSNSSNITMSHH103 pKa = 4.84ITVHH107 pKa = 6.58GSLDD111 pKa = 3.63NDD113 pKa = 4.11PANDD117 pKa = 3.63GMGMMVRR124 pKa = 11.84NVAGFSISDD133 pKa = 3.52STFGDD138 pKa = 4.4LYY140 pKa = 10.94RR141 pKa = 11.84GIVLQNNTNATVQNNTIKK159 pKa = 10.11TIRR162 pKa = 11.84SDD164 pKa = 4.15GIISIANDD172 pKa = 4.96GITIAGNHH180 pKa = 6.24IGEE183 pKa = 4.45FHH185 pKa = 7.16PKK187 pKa = 10.47LGDD190 pKa = 3.46HH191 pKa = 7.31ADD193 pKa = 5.39AIQFWNTGQTKK204 pKa = 9.15GQTDD208 pKa = 3.44ITVKK212 pKa = 11.02DD213 pKa = 3.52NVIYY217 pKa = 10.47QSYY220 pKa = 10.35FSGIDD225 pKa = 3.11QTGVQGIFMSDD236 pKa = 3.28PLSYY240 pKa = 10.48GYY242 pKa = 11.18KK243 pKa = 10.25NVLIQNNLLYY253 pKa = 11.27SNDD256 pKa = 3.37AYY258 pKa = 11.5NGIFVNGATGAQIIGNTVLSHH279 pKa = 6.88SSDD282 pKa = 3.44SKK284 pKa = 11.31LFWIDD289 pKa = 3.34VLNSDD294 pKa = 5.09QVSLQDD300 pKa = 3.63NLTDD304 pKa = 5.79RR305 pKa = 11.84IITSNVTNFAQSGNVDD321 pKa = 4.02FSVNAGARR329 pKa = 11.84AMLPNLANPAGVEE342 pKa = 4.08DD343 pKa = 4.71LVASGVGYY351 pKa = 10.03HH352 pKa = 6.84LPPPPTPITPPAPLPIDD369 pKa = 3.68FTGDD373 pKa = 3.39DD374 pKa = 4.24ANNLINANAADD385 pKa = 3.79NNIDD389 pKa = 3.63GGKK392 pKa = 10.21GADD395 pKa = 3.54AMYY398 pKa = 11.0GLAGNDD404 pKa = 3.4HH405 pKa = 6.92YY406 pKa = 11.38IVDD409 pKa = 3.92NAGDD413 pKa = 3.79RR414 pKa = 11.84VFEE417 pKa = 4.51DD418 pKa = 3.76ANAGHH423 pKa = 7.05DD424 pKa = 3.85VVSTSVTYY432 pKa = 10.62SLQAGTSVEE441 pKa = 4.12VLQVLGPDD449 pKa = 3.07TTTAINLTGNEE460 pKa = 4.33LANTLIGNAGKK471 pKa = 10.35NILDD475 pKa = 4.04GGAGADD481 pKa = 4.1EE482 pKa = 4.19LHH484 pKa = 6.62GLAGDD489 pKa = 3.43DD490 pKa = 4.82CYY492 pKa = 11.03FVDD495 pKa = 3.71HH496 pKa = 7.09AGDD499 pKa = 3.35HH500 pKa = 5.97VYY502 pKa = 10.53EE503 pKa = 4.42VAGEE507 pKa = 4.56GYY509 pKa = 8.41DD510 pKa = 3.73TVCASVSYY518 pKa = 10.63ALQAGTSIEE527 pKa = 4.08VLQTNDD533 pKa = 2.98VAGTAAINLTGNEE546 pKa = 4.06LVNTVFGNGGANILDD561 pKa = 4.13GGAGADD567 pKa = 3.49KK568 pKa = 10.91LQGFGGDD575 pKa = 3.62DD576 pKa = 3.52SYY578 pKa = 12.11LVDD581 pKa = 3.51NARR584 pKa = 11.84DD585 pKa = 3.75SVYY588 pKa = 10.37EE589 pKa = 3.8AAGGGRR595 pKa = 11.84DD596 pKa = 3.44TVSASVSYY604 pKa = 9.04TLQPGTYY611 pKa = 9.9VEE613 pKa = 4.3VLQTNDD619 pKa = 3.56DD620 pKa = 4.09GGTATLDD627 pKa = 3.38LSGNDD632 pKa = 3.23FANVVRR638 pKa = 11.84GNAGNNTLQGRR649 pKa = 11.84GGDD652 pKa = 3.74DD653 pKa = 3.3VLIGLGGDD661 pKa = 3.33DD662 pKa = 4.28RR663 pKa = 11.84LYY665 pKa = 11.19GQAGNDD671 pKa = 3.36TFVFAPGFGRR681 pKa = 11.84DD682 pKa = 3.5MVGDD686 pKa = 4.57FAAHH690 pKa = 5.97SAGGGDD696 pKa = 3.7LVAFQGKK703 pKa = 9.5AFSGFDD709 pKa = 3.59DD710 pKa = 4.49LMAHH714 pKa = 7.46AAQKK718 pKa = 9.17GTDD721 pKa = 3.38VVFTLDD727 pKa = 3.57ANTSLTLHH735 pKa = 5.96NVQLAALSAQDD746 pKa = 3.32FTFAA750 pKa = 4.84

Molecular weight: 77.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E9MP55|A0A0E9MP55_9SPHN Uncharacterized protein OS=Sphingomonas changbaiensis NBRC 104936 OX=1219043 GN=SCH01S_28_00750 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.32VIAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.89VLSAA44 pKa = 4.11

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3151

0

3151

976675

41

2672

310.0

33.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.403 ± 0.066

0.75 ± 0.013

5.932 ± 0.034

5.51 ± 0.042

3.555 ± 0.03

8.95 ± 0.052

1.972 ± 0.02

4.858 ± 0.026

3.011 ± 0.035

10.0 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.262 ± 0.022

2.504 ± 0.033

5.541 ± 0.034

3.102 ± 0.027

7.597 ± 0.052

5.084 ± 0.036

5.163 ± 0.033

7.197 ± 0.036

1.431 ± 0.021

2.18 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski