Nonomuraea jiangxiensis
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10809 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G9UM79|A0A1G9UM79_9ACTN Uncharacterized protein OS=Nonomuraea jiangxiensis OX=633440 GN=SAMN05421869_14917 PE=4 SV=1
MM1 pKa = 7.13 TVVTHH6 pKa = 7.7 DD7 pKa = 5.51 DD8 pKa = 3.93 DD9 pKa = 5.62 FPWIYY14 pKa = 10.8 NPYY17 pKa = 7.86 WDD19 pKa = 4.7 KK20 pKa = 11.21 PEE22 pKa = 4.23 PPIPFQKK29 pKa = 10.09 VSGYY33 pKa = 9.59 EE34 pKa = 3.33 WVYY37 pKa = 10.85 PDD39 pKa = 4.53 KK40 pKa = 10.93 TDD42 pKa = 4.3 SDD44 pKa = 4.47 GNYY47 pKa = 9.16 IWAKK51 pKa = 10.73 DD52 pKa = 3.82 NSDD55 pKa = 3.69 DD56 pKa = 4.26
Molecular weight: 6.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.872
IPC_protein 3.821
Toseland 3.592
ProMoST 3.935
Dawson 3.846
Bjellqvist 4.075
Wikipedia 3.834
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.973
DTASelect 4.266
Thurlkill 3.681
EMBOSS 3.834
Sillero 3.948
Patrickios 1.926
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A1G8FWG9|A0A1G8FWG9_9ACTN Bifunctional purine biosynthesis protein PurH OS=Nonomuraea jiangxiensis OX=633440 GN=purH PE=3 SV=1
MM1 pKa = 7.59 LNGRR5 pKa = 11.84 LTRR8 pKa = 11.84 VLTGRR13 pKa = 11.84 LTGVLARR20 pKa = 11.84 EE21 pKa = 4.21 LTGVLARR28 pKa = 11.84 EE29 pKa = 4.35 LSRR32 pKa = 11.84 RR33 pKa = 11.84 LTGMLSRR40 pKa = 11.84 RR41 pKa = 11.84 LSGRR45 pKa = 11.84 LAHH48 pKa = 6.63 RR49 pKa = 11.84 PSGRR53 pKa = 11.84 LARR56 pKa = 11.84 LLSGRR61 pKa = 11.84 LAPLLSRR68 pKa = 11.84 RR69 pKa = 11.84 LTRR72 pKa = 11.84 VLSGRR77 pKa = 11.84 LAGVLSGRR85 pKa = 11.84 RR86 pKa = 11.84 GKK88 pKa = 10.26 LRR90 pKa = 11.84 RR91 pKa = 11.84 LHH93 pKa = 6.7 RR94 pKa = 11.84 GLGSGLRR101 pKa = 11.84 RR102 pKa = 11.84 RR103 pKa = 11.84 SRR105 pKa = 11.84 TRR107 pKa = 11.84 SLL109 pKa = 3.2
Molecular weight: 12.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.506
IPC2_protein 11.14
IPC_protein 12.764
Toseland 12.925
ProMoST 13.422
Dawson 12.925
Bjellqvist 12.925
Wikipedia 13.408
Rodwell 12.442
Grimsley 12.969
Solomon 13.422
Lehninger 13.32
Nozaki 12.925
DTASelect 12.925
Thurlkill 12.925
EMBOSS 13.422
Sillero 12.925
Patrickios 12.149
IPC_peptide 13.422
IPC2_peptide 12.413
IPC2.peptide.svr19 9.186
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10809
0
10809
3474277
27
5397
321.4
34.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.184 ± 0.037
0.762 ± 0.007
5.745 ± 0.019
5.557 ± 0.024
2.84 ± 0.014
9.33 ± 0.024
2.245 ± 0.013
3.571 ± 0.015
1.935 ± 0.019
10.643 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.88 ± 0.009
1.845 ± 0.015
6.145 ± 0.02
2.752 ± 0.014
8.23 ± 0.024
5.006 ± 0.017
5.931 ± 0.019
8.623 ± 0.025
1.605 ± 0.011
2.169 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here