Gordonia phage Vivi2 
Average proteome isoelectric point is 5.99 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A142K9W7|A0A142K9W7_9CAUD Uncharacterized protein OS=Gordonia phage Vivi2 OX=1821564 GN=68 PE=4 SV=1MM1 pKa = 7.52  SWTEE5 pKa = 3.81  DD6 pKa = 3.54  CNACGHH12 pKa = 5.69  THH14 pKa = 7.4  PDD16 pKa = 3.65  EE17 pKa = 4.91  CDD19 pKa = 3.81  CPCGCTMHH27 pKa = 7.33  PVDD30 pKa = 3.83  TWDD33 pKa = 3.45  MTEE36 pKa = 4.44  LPGDD40 pKa = 3.66  EE41 pKa = 4.19  
 4.57 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.901 
IPC2_protein 3.834 
IPC_protein 3.706 
Toseland    3.516 
ProMoST     3.846 
Dawson      3.719 
Bjellqvist  3.986 
Wikipedia   3.694 
Rodwell     3.554 
Grimsley    3.452 
Solomon     3.681 
Lehninger   3.643 
Nozaki      3.872 
DTASelect   4.05 
Thurlkill   3.605 
EMBOSS      3.694 
Sillero     3.846 
Patrickios  0.006 
IPC_peptide 3.681 
IPC2_peptide  3.808 
IPC2.peptide.svr19  3.864 
 Protein with the highest isoelectric point: 
>tr|A0A142K9R7|A0A142K9R7_9CAUD HNH endonuclease OS=Gordonia phage Vivi2 OX=1821564 GN=18 PE=4 SV=1MM1 pKa = 7.26  GWLLFGAVFIPVRR14 pKa = 11.84  IAKK17 pKa = 9.9  IIEE20 pKa = 4.48  DD21 pKa = 3.61  ALSPRR26 pKa = 11.84  RR27 pKa = 11.84  RR28 pKa = 11.84  RR29 pKa = 11.84  EE30 pKa = 3.9  LIQQQTRR37 pKa = 11.84  DD38 pKa = 3.68  EE39 pKa = 4.24  VAAAAEE45 pKa = 3.96  RR46 pKa = 11.84  SEE48 pKa = 4.23  EE49 pKa = 3.96  YY50 pKa = 10.34  RR51 pKa = 11.84  RR52 pKa = 11.84  TRR54 pKa = 11.84  YY55 pKa = 10.12  GLL57 pKa = 3.49  
 6.7 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.29 
IPC2_protein 9.428 
IPC_protein 10.511 
Toseland    10.16 
ProMoST     10.101 
Dawson      10.394 
Bjellqvist  10.204 
Wikipedia   10.687 
Rodwell     10.335 
Grimsley    10.496 
Solomon     10.54 
Lehninger   10.496 
Nozaki      10.116 
DTASelect   10.204 
Thurlkill   10.233 
EMBOSS      10.584 
Sillero     10.321 
Patrickios  10.16 
IPC_peptide 10.526 
IPC2_peptide  9.224 
IPC2.peptide.svr19  8.475 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        89 
0
89 
18766
37
1603
210.9
22.95
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        11.974 ± 0.389
0.837 ± 0.109
7.625 ± 0.458
5.611 ± 0.361
2.489 ± 0.168
8.681 ± 0.369
2.163 ± 0.18
4.497 ± 0.275
2.387 ± 0.207
7.386 ± 0.261
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.185 ± 0.096
2.824 ± 0.188
6.139 ± 0.213
3.73 ± 0.183
7.647 ± 0.434
4.7 ± 0.221
6.741 ± 0.307
7.961 ± 0.265
2.126 ± 0.121
2.297 ± 0.143
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here