Gordonia phage Vivi2
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142K9W7|A0A142K9W7_9CAUD Uncharacterized protein OS=Gordonia phage Vivi2 OX=1821564 GN=68 PE=4 SV=1
MM1 pKa = 7.52 SWTEE5 pKa = 3.81 DD6 pKa = 3.54 CNACGHH12 pKa = 5.69 THH14 pKa = 7.4 PDD16 pKa = 3.65 EE17 pKa = 4.91 CDD19 pKa = 3.81 CPCGCTMHH27 pKa = 7.33 PVDD30 pKa = 3.83 TWDD33 pKa = 3.45 MTEE36 pKa = 4.44 LPGDD40 pKa = 3.66 EE41 pKa = 4.19
Molecular weight: 4.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.901
IPC2_protein 3.834
IPC_protein 3.706
Toseland 3.516
ProMoST 3.846
Dawson 3.719
Bjellqvist 3.986
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.452
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.846
Patrickios 0.006
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.864
Protein with the highest isoelectric point:
>tr|A0A142K9R7|A0A142K9R7_9CAUD HNH endonuclease OS=Gordonia phage Vivi2 OX=1821564 GN=18 PE=4 SV=1
MM1 pKa = 7.26 GWLLFGAVFIPVRR14 pKa = 11.84 IAKK17 pKa = 9.9 IIEE20 pKa = 4.48 DD21 pKa = 3.61 ALSPRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 EE30 pKa = 3.9 LIQQQTRR37 pKa = 11.84 DD38 pKa = 3.68 EE39 pKa = 4.24 VAAAAEE45 pKa = 3.96 RR46 pKa = 11.84 SEE48 pKa = 4.23 EE49 pKa = 3.96 YY50 pKa = 10.34 RR51 pKa = 11.84 RR52 pKa = 11.84 TRR54 pKa = 11.84 YY55 pKa = 10.12 GLL57 pKa = 3.49
Molecular weight: 6.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.29
IPC2_protein 9.428
IPC_protein 10.511
Toseland 10.16
ProMoST 10.101
Dawson 10.394
Bjellqvist 10.204
Wikipedia 10.687
Rodwell 10.335
Grimsley 10.496
Solomon 10.54
Lehninger 10.496
Nozaki 10.116
DTASelect 10.204
Thurlkill 10.233
EMBOSS 10.584
Sillero 10.321
Patrickios 10.16
IPC_peptide 10.526
IPC2_peptide 9.224
IPC2.peptide.svr19 8.475
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
89
0
89
18766
37
1603
210.9
22.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.974 ± 0.389
0.837 ± 0.109
7.625 ± 0.458
5.611 ± 0.361
2.489 ± 0.168
8.681 ± 0.369
2.163 ± 0.18
4.497 ± 0.275
2.387 ± 0.207
7.386 ± 0.261
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.185 ± 0.096
2.824 ± 0.188
6.139 ± 0.213
3.73 ± 0.183
7.647 ± 0.434
4.7 ± 0.221
6.741 ± 0.307
7.961 ± 0.265
2.126 ± 0.121
2.297 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here