Carrot Ch virus 2
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0P9V6|A0A0A0P9V6_9VIRU ORF1 OS=Carrot Ch virus 2 OX=1425362 PE=4 SV=1
MM1 pKa = 7.35 SLVDD5 pKa = 3.77 INKK8 pKa = 9.24 FCALVSKK15 pKa = 10.39 GASSVEE21 pKa = 4.33 AIDD24 pKa = 4.08 KK25 pKa = 10.95 GNIYY29 pKa = 10.66 GDD31 pKa = 3.94 SSWLKK36 pKa = 9.7 FNQVSAIRR44 pKa = 11.84 KK45 pKa = 8.08 FEE47 pKa = 4.61 SNISIEE53 pKa = 4.71 LDD55 pKa = 3.78 DD56 pKa = 4.63 GDD58 pKa = 4.24 KK59 pKa = 11.33 SLVIPNIPLVSSFEE73 pKa = 4.1 VATVKK78 pKa = 10.68 KK79 pKa = 10.27 SLKK82 pKa = 9.69 NANFLHH88 pKa = 6.65 LGGIVISIQALFAEE102 pKa = 4.75 NKK104 pKa = 9.65 GVTGTAVLADD114 pKa = 3.87 KK115 pKa = 10.53 RR116 pKa = 11.84 WNNLDD121 pKa = 3.25 QAIIGAFHH129 pKa = 6.98 FNLDD133 pKa = 3.03 KK134 pKa = 11.03 RR135 pKa = 11.84 RR136 pKa = 11.84 ADD138 pKa = 4.21 FIMRR142 pKa = 11.84 PNFDD146 pKa = 3.06 VSLNDD151 pKa = 4.19 PMLCDD156 pKa = 5.27 SISLMLKK163 pKa = 10.37 FEE165 pKa = 4.39 NLDD168 pKa = 3.6 MQVSSIPINVSVGLIARR185 pKa = 11.84 FYY187 pKa = 9.67 NTIDD191 pKa = 3.56 PGIKK195 pKa = 9.96 FADD198 pKa = 4.41 EE199 pKa = 3.95 EE200 pKa = 4.7 LKK202 pKa = 9.78 MQGLIEE208 pKa = 4.94 AEE210 pKa = 4.65 GIDD213 pKa = 4.4 KK214 pKa = 11.11 NDD216 pKa = 3.64 MLHH219 pKa = 7.18 SIGDD223 pKa = 3.83 LADD226 pKa = 3.68 TFSNPAIFNPTIVNNRR242 pKa = 11.84 KK243 pKa = 9.03 YY244 pKa = 10.98 DD245 pKa = 3.44 RR246 pKa = 11.84 GLFKK250 pKa = 11.05 DD251 pKa = 3.55 KK252 pKa = 11.35 GFIKK256 pKa = 10.19 QIRR259 pKa = 11.84 GSRR262 pKa = 11.84 SDD264 pKa = 3.25 DD265 pKa = 3.37 SKK267 pKa = 11.22 ALRR270 pKa = 11.84 RR271 pKa = 11.84 VGRR274 pKa = 11.84 FNAPSSSRR282 pKa = 11.84 RR283 pKa = 11.84 FDD285 pKa = 3.58 QIKK288 pKa = 10.26 VDD290 pKa = 3.59 PLFYY294 pKa = 11.04 DD295 pKa = 3.79 KK296 pKa = 10.72 IKK298 pKa = 10.68 RR299 pKa = 11.84 GNINSWLNNEE309 pKa = 4.4 LPKK312 pKa = 10.61 SSEE315 pKa = 4.13 RR316 pKa = 11.84 EE317 pKa = 3.67 EE318 pKa = 3.9 EE319 pKa = 4.4 RR320 pKa = 11.84 YY321 pKa = 10.19 SEE323 pKa = 4.18 TGSEE327 pKa = 4.03 GRR329 pKa = 11.84 VEE331 pKa = 4.21 IVEE334 pKa = 4.28 HH335 pKa = 6.26
Molecular weight: 37.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.964
IPC2_protein 5.906
IPC_protein 5.944
Toseland 6.262
ProMoST 6.02
Dawson 6.16
Bjellqvist 6.173
Wikipedia 6.16
Rodwell 6.135
Grimsley 6.402
Solomon 6.173
Lehninger 6.16
Nozaki 6.402
DTASelect 6.59
Thurlkill 6.561
EMBOSS 6.547
Sillero 6.504
Patrickios 4.825
IPC_peptide 6.186
IPC2_peptide 6.389
IPC2.peptide.svr19 6.592
Protein with the highest isoelectric point:
>tr|A0A0A0P507|A0A0A0P507_9VIRU ORF2 OS=Carrot Ch virus 2 OX=1425362 PE=4 SV=1
MM1 pKa = 7.8 SYY3 pKa = 11.19 LSLQRR8 pKa = 11.84 EE9 pKa = 3.98 RR10 pKa = 11.84 RR11 pKa = 11.84 SDD13 pKa = 3.37 IVKK16 pKa = 10.28 LALKK20 pKa = 10.57 EE21 pKa = 3.95 EE22 pKa = 4.16 LRR24 pKa = 11.84 SLNTEE29 pKa = 3.35 IDD31 pKa = 3.42 EE32 pKa = 4.47 KK33 pKa = 10.8 GTYY36 pKa = 9.2 FKK38 pKa = 10.79 QVLEE42 pKa = 4.09 YY43 pKa = 10.57 YY44 pKa = 10.25 IMYY47 pKa = 10.08 FVLNMAEE54 pKa = 4.54 MGTSPDD60 pKa = 3.04 TVYY63 pKa = 10.66 PSEE66 pKa = 4.09 NFEE69 pKa = 4.56 LKK71 pKa = 10.27 PVTMKK76 pKa = 10.84 SGDD79 pKa = 3.6 EE80 pKa = 3.94 PKK82 pKa = 10.64 TFILKK87 pKa = 9.63 MNLITCIDD95 pKa = 4.76 RR96 pKa = 11.84 IQDD99 pKa = 3.52 LSEE102 pKa = 3.86 NRR104 pKa = 11.84 VDD106 pKa = 6.05 LGDD109 pKa = 3.48 LTLRR113 pKa = 11.84 SFMRR117 pKa = 11.84 PFADD121 pKa = 2.98 IAKK124 pKa = 9.67 KK125 pKa = 9.89 QLQKK129 pKa = 10.8 RR130 pKa = 11.84 GVDD133 pKa = 4.0 GILARR138 pKa = 11.84 KK139 pKa = 8.58 YY140 pKa = 10.27 PRR142 pKa = 11.84 VCRR145 pKa = 11.84 GAKK148 pKa = 10.19 HH149 pKa = 5.88 MGFDD153 pKa = 4.74 FNQDD157 pKa = 3.43 LQSSEE162 pKa = 4.11 LSNSEE167 pKa = 3.64 MRR169 pKa = 11.84 VKK171 pKa = 10.72 QNLGRR176 pKa = 11.84 VMFNRR181 pKa = 11.84 GLKK184 pKa = 9.07 VANLEE189 pKa = 4.18 EE190 pKa = 4.39 SSSIDD195 pKa = 3.54 FEE197 pKa = 4.47
Molecular weight: 22.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.746
IPC2_protein 6.942
IPC_protein 7.322
Toseland 7.015
ProMoST 7.059
Dawson 7.922
Bjellqvist 7.688
Wikipedia 7.863
Rodwell 7.892
Grimsley 6.956
Solomon 8.156
Lehninger 8.17
Nozaki 7.98
DTASelect 7.922
Thurlkill 7.966
EMBOSS 8.17
Sillero 8.229
Patrickios 4.635
IPC_peptide 8.141
IPC2_peptide 7.059
IPC2.peptide.svr19 7.802
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2388
197
1856
796.0
91.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.276 ± 0.42
1.675 ± 0.447
7.328 ± 0.217
6.826 ± 0.362
6.784 ± 0.632
5.36 ± 0.249
1.424 ± 0.295
7.161 ± 0.622
9.213 ± 0.843
9.757 ± 0.418
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.722 ± 0.586
4.983 ± 0.86
3.141 ± 0.039
2.806 ± 0.31
4.899 ± 0.891
8.501 ± 0.372
2.889 ± 0.298
5.318 ± 0.243
0.754 ± 0.192
3.183 ± 0.474
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here