Paracoccus sp. 1011MAR3C25
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4491 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A418SWM4|A0A418SWM4_9RHOB Iron-sulfur cluster assembly accessory protein OS=Paracoccus sp. 1011MAR3C25 OX=1675788 GN=D3P04_09835 PE=3 SV=1
MM1 pKa = 7.6 AHH3 pKa = 5.39 PHH5 pKa = 6.21 IYY7 pKa = 9.91 IEE9 pKa = 4.43 ASLDD13 pKa = 3.71 LIYY16 pKa = 10.92 NEE18 pKa = 4.48 KK19 pKa = 11.22 GEE21 pKa = 4.13 LTSFGVEE28 pKa = 3.45 WAYY31 pKa = 11.17 DD32 pKa = 3.66 EE33 pKa = 5.8 LYY35 pKa = 10.98 SLLIIEE41 pKa = 5.36 DD42 pKa = 4.09 FSFDD46 pKa = 3.46 QDD48 pKa = 3.1 GDD50 pKa = 4.09 GVLTPEE56 pKa = 4.56 EE57 pKa = 4.18 NEE59 pKa = 4.18 MIQGFDD65 pKa = 4.15 ADD67 pKa = 4.35 WEE69 pKa = 4.37 PDD71 pKa = 3.16 FDD73 pKa = 3.83 GRR75 pKa = 11.84 LYY77 pKa = 10.46 PSVDD81 pKa = 3.42 GQPVAMEE88 pKa = 3.96 PVRR91 pKa = 11.84 DD92 pKa = 4.37 FTAEE96 pKa = 3.82 YY97 pKa = 10.19 RR98 pKa = 11.84 DD99 pKa = 3.59 GRR101 pKa = 11.84 LVSYY105 pKa = 10.04 HH106 pKa = 6.47 LHH108 pKa = 6.48 PLSTPLPADD117 pKa = 3.2 KK118 pKa = 10.89 SLTIQVYY125 pKa = 9.5 DD126 pKa = 3.49 PEE128 pKa = 5.0 FYY130 pKa = 11.38 VHH132 pKa = 6.44 FTLPEE137 pKa = 4.0 PPEE140 pKa = 3.96 AKK142 pKa = 10.45 GRR144 pKa = 11.84 DD145 pKa = 3.56 DD146 pKa = 5.79 CEE148 pKa = 3.82 ITLIPGDD155 pKa = 4.15 PNAAPAAYY163 pKa = 8.88 RR164 pKa = 11.84 QAVEE168 pKa = 4.71 ALLGSNASNDD178 pKa = 3.67 DD179 pKa = 3.73 ADD181 pKa = 4.9 LVTVDD186 pKa = 4.22 IGAAGADD193 pKa = 3.85 EE194 pKa = 4.17 ARR196 pKa = 11.84 IRR198 pKa = 11.84 CGTGLAEE205 pKa = 4.14
Molecular weight: 22.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.884
IPC_protein 3.884
Toseland 3.681
ProMoST 4.012
Dawson 3.859
Bjellqvist 4.05
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.19
Thurlkill 3.719
EMBOSS 3.795
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.859
IPC2_peptide 3.986
IPC2.peptide.svr19 3.908
Protein with the highest isoelectric point:
>tr|A0A418T4K1|A0A418T4K1_9RHOB Damage-inducible protein DinB OS=Paracoccus sp. 1011MAR3C25 OX=1675788 GN=D3P04_04355 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.12 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.69 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4491
0
4491
1420025
28
4619
316.2
34.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.931 ± 0.045
0.878 ± 0.011
5.975 ± 0.031
5.951 ± 0.036
3.627 ± 0.024
8.78 ± 0.039
2.099 ± 0.02
5.697 ± 0.026
2.84 ± 0.028
10.166 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.82 ± 0.018
2.669 ± 0.021
5.235 ± 0.03
3.301 ± 0.019
7.128 ± 0.04
5.364 ± 0.025
5.198 ± 0.023
6.767 ± 0.028
1.418 ± 0.014
2.157 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here