Alphaproteobacteria bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; unclassified Alphaproteobacteria

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5432 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q5XCX3|A0A4Q5XCX3_9PROT DNA ligase (NAD(+)) (Fragment) OS=Alphaproteobacteria bacterium OX=1913988 GN=ligA PE=3 SV=1
MM1 pKa = 7.96RR2 pKa = 11.84NLLVLGFIAAVSAAPALAQEE22 pKa = 4.5VTISGNVGLTTDD34 pKa = 3.56YY35 pKa = 11.4AFRR38 pKa = 11.84GVSQSDD44 pKa = 3.69EE45 pKa = 4.14GPAIQGGLDD54 pKa = 3.23ATFGDD59 pKa = 3.56SGFYY63 pKa = 10.2VGTWASSINFGTGGSNMEE81 pKa = 4.04LDD83 pKa = 3.7VYY85 pKa = 10.93GGYY88 pKa = 10.5KK89 pKa = 9.95FAIGGVNVDD98 pKa = 3.64VGALGYY104 pKa = 9.74LYY106 pKa = 10.1PGAADD111 pKa = 5.26DD112 pKa = 4.89GAEE115 pKa = 3.92LDD117 pKa = 3.71FWEE120 pKa = 5.96LYY122 pKa = 10.34AKK124 pKa = 10.42PSIALSDD131 pKa = 3.67QFTLGAAFAYY141 pKa = 10.24SPEE144 pKa = 4.14FTGEE148 pKa = 3.99SGDD151 pKa = 3.41AFYY154 pKa = 11.66YY155 pKa = 9.74EE156 pKa = 4.37VNGAFAATPEE166 pKa = 4.4LSFSGAVGVQTVDD179 pKa = 2.6TDD181 pKa = 3.67GFFAGEE187 pKa = 4.15DD188 pKa = 3.95KK189 pKa = 10.54YY190 pKa = 8.22TTWNVGGTYY199 pKa = 10.27SAFGLGFDD207 pKa = 4.56LRR209 pKa = 11.84YY210 pKa = 10.26HH211 pKa = 5.84GTDD214 pKa = 2.83VDD216 pKa = 4.0NVAIYY221 pKa = 10.18DD222 pKa = 3.67DD223 pKa = 4.32RR224 pKa = 11.84VIFSIKK230 pKa = 9.83KK231 pKa = 9.78ALL233 pKa = 3.56

Molecular weight: 24.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q5XYL8|A0A4Q5XYL8_9PROT Uncharacterized protein OS=Alphaproteobacteria bacterium OX=1913988 GN=EON61_02755 PE=4 SV=1
MM1 pKa = 7.74AALTSKK7 pKa = 10.22KK8 pKa = 9.56RR9 pKa = 11.84ARR11 pKa = 11.84NVASVDD17 pKa = 3.07ARR19 pKa = 11.84LGNFRR24 pKa = 11.84FSTHH28 pKa = 5.93LAISAGGLLRR38 pKa = 11.84VSALVGVILAGTTGIVWVATTPARR62 pKa = 11.84PPRR65 pKa = 11.84RR66 pKa = 11.84HH67 pKa = 6.8RR68 pKa = 11.84ILTT71 pKa = 3.67

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5432

0

5432

1485962

23

2807

273.6

29.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.637 ± 0.043

0.785 ± 0.011

5.776 ± 0.028

5.609 ± 0.028

3.731 ± 0.023

8.599 ± 0.033

1.853 ± 0.014

5.067 ± 0.024

4.047 ± 0.029

9.233 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.016

2.87 ± 0.022

5.341 ± 0.028

3.196 ± 0.018

6.581 ± 0.038

5.522 ± 0.024

5.562 ± 0.033

7.261 ± 0.025

1.456 ± 0.015

2.277 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski