Microbacterium phage Phractured

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Eekayvirinae; Akonivirus; unclassified Akonivirus

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3T3W9|A0A6M3T3W9_9CAUD Minor tail protein OS=Microbacterium phage Phractured OX=2725605 GN=35 PE=4 SV=1
MM1 pKa = 7.45SLHH4 pKa = 6.89PEE6 pKa = 4.36DD7 pKa = 4.18KK8 pKa = 10.95AQLIQLLNDD17 pKa = 3.93TTCLKK22 pKa = 10.45CGKK25 pKa = 9.58QMYY28 pKa = 9.36WDD30 pKa = 4.79DD31 pKa = 3.98SDD33 pKa = 3.66TGEE36 pKa = 4.13GSVYY40 pKa = 10.77SDD42 pKa = 3.93AGLKK46 pKa = 7.01EE47 pKa = 3.97TRR49 pKa = 11.84ISGFCEE55 pKa = 3.71HH56 pKa = 6.98CFDD59 pKa = 4.52EE60 pKa = 4.98VTRR63 pKa = 11.84PPDD66 pKa = 3.37EE67 pKa = 5.26PEE69 pKa = 5.39DD70 pKa = 3.83DD71 pKa = 5.07RR72 pKa = 11.84DD73 pKa = 3.4WKK75 pKa = 10.82DD76 pKa = 3.47YY77 pKa = 11.01YY78 pKa = 10.01EE79 pKa = 3.97QSIIDD84 pKa = 3.59EE85 pKa = 4.8EE86 pKa = 4.51YY87 pKa = 11.01AKK89 pKa = 11.02DD90 pKa = 3.59RR91 pKa = 11.84FSDD94 pKa = 4.4LIEE97 pKa = 4.46DD98 pKa = 4.37RR99 pKa = 11.84EE100 pKa = 4.1PDD102 pKa = 3.32EE103 pKa = 6.13HH104 pKa = 9.02SDD106 pKa = 3.63EE107 pKa = 4.33LL108 pKa = 4.61

Molecular weight: 12.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3T3R3|A0A6M3T3R3_9CAUD Uncharacterized protein OS=Microbacterium phage Phractured OX=2725605 GN=24 PE=4 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84FTRR5 pKa = 11.84RR6 pKa = 11.84TYY8 pKa = 9.52KK9 pKa = 10.44AAVRR13 pKa = 11.84RR14 pKa = 11.84AFDD17 pKa = 3.62LEE19 pKa = 4.39LYY21 pKa = 10.16FYY23 pKa = 10.6RR24 pKa = 11.84GWCAGFCGPHH34 pKa = 7.5KK35 pKa = 10.26YY36 pKa = 9.6RR37 pKa = 11.84QAVRR41 pKa = 11.84TTNLLLARR49 pKa = 11.84RR50 pKa = 11.84KK51 pKa = 9.57KK52 pKa = 10.82ALGFF56 pKa = 4.04

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

17030

38

4489

309.6

34.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.542 ± 0.383

0.47 ± 0.169

6.606 ± 0.222

6.618 ± 0.396

3.23 ± 0.133

7.499 ± 0.363

1.656 ± 0.173

4.944 ± 0.193

4.398 ± 0.401

8.297 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.525 ± 0.14

4.639 ± 0.187

4.586 ± 0.167

4.51 ± 0.27

5.989 ± 0.375

6.224 ± 0.338

6.806 ± 0.315

6.506 ± 0.228

1.844 ± 0.127

3.112 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski