Apis mellifera (Honeybee)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19071 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7M7GQ61|A0A7M7GQ61_APIME Uncharacterized protein OS=Apis mellifera OX=7460 PE=3 SV=1
MM1 pKa = 7.11VVLSNFLAPQEE12 pKa = 4.56GIRR15 pKa = 11.84HH16 pKa = 5.6EE17 pKa = 4.32EE18 pKa = 3.96QNTTVYY24 pKa = 10.76INDD27 pKa = 3.7RR28 pKa = 11.84EE29 pKa = 4.38VGKK32 pKa = 8.77GTLYY36 pKa = 9.3ITEE39 pKa = 4.71SLLSWVNYY47 pKa = 7.76DD48 pKa = 3.57TQQGFSLEE56 pKa = 4.16YY57 pKa = 10.02PHH59 pKa = 7.27ISLHH63 pKa = 6.32AISRR67 pKa = 11.84DD68 pKa = 3.7EE69 pKa = 3.97QVHH72 pKa = 5.94PRR74 pKa = 11.84QCLYY78 pKa = 10.85IMVDD82 pKa = 3.51AKK84 pKa = 11.31VDD86 pKa = 3.89LPDD89 pKa = 3.65VSLSPASDD97 pKa = 3.15SGSEE101 pKa = 4.23NEE103 pKa = 5.53FEE105 pKa = 5.55DD106 pKa = 4.25ADD108 pKa = 4.15TPITEE113 pKa = 4.27MRR115 pKa = 11.84FAPDD119 pKa = 3.02NTNNLEE125 pKa = 4.54AMFQAMNQCQALHH138 pKa = 7.44PDD140 pKa = 3.88PQDD143 pKa = 3.37SFSDD147 pKa = 3.74AEE149 pKa = 3.83EE150 pKa = 5.32DD151 pKa = 3.31IYY153 pKa = 11.51EE154 pKa = 4.54DD155 pKa = 4.26AEE157 pKa = 4.14EE158 pKa = 5.55DD159 pKa = 3.94DD160 pKa = 4.26FEE162 pKa = 6.75HH163 pKa = 7.1YY164 pKa = 10.65DD165 pKa = 3.6VGAGDD170 pKa = 4.46APYY173 pKa = 10.34ILPTEE178 pKa = 4.31QIGTNHH184 pKa = 6.69NGTEE188 pKa = 4.34ADD190 pKa = 3.46DD191 pKa = 4.6AMDD194 pKa = 3.71IEE196 pKa = 4.99AGQFEE201 pKa = 4.76DD202 pKa = 5.49AEE204 pKa = 4.39EE205 pKa = 4.16DD206 pKa = 3.59LL207 pKa = 5.34

Molecular weight: 23.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7M7FYV2|A0A7M7FYV2_APIME 39S ribosomal protein L59 mitochondrial OS=Apis mellifera OX=7460 GN=725669 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 7.85RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.6MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9955

9116

19071

15795738

9

25204

828.3

93.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.862 ± 0.018

1.912 ± 0.045

5.303 ± 0.015

7.498 ± 0.053

3.286 ± 0.015

5.607 ± 0.024

2.391 ± 0.012

6.001 ± 0.019

6.651 ± 0.037

8.549 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.119 ± 0.009

5.185 ± 0.027

5.357 ± 0.032

4.48 ± 0.021

5.542 ± 0.022

8.327 ± 0.027

6.13 ± 0.026

5.836 ± 0.017

1.013 ± 0.006

2.951 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski