Duck hepatitis B virus (strain United States/DHBV-16) (DHBV)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Avihepadnavirus; Duck hepatitis B virus

Average proteome isoelectric point is 8.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03145-2|HBSAG-2_DHBV1 Isoform of P03145 Isoform S of Large envelope protein OS=Duck hepatitis B virus (strain United States/DHBV-16) OX=489543 GN=S PE=1 SV=4
MM1 pKa = 7.08GQHH4 pKa = 6.83PAKK7 pKa = 10.86SMDD10 pKa = 2.88VRR12 pKa = 11.84RR13 pKa = 11.84IEE15 pKa = 4.32GGEE18 pKa = 3.69ILLNQLAGRR27 pKa = 11.84MIPKK31 pKa = 8.61GTLTWSGKK39 pKa = 10.11FPTLDD44 pKa = 2.95HH45 pKa = 6.62VLDD48 pKa = 4.2HH49 pKa = 5.99VQTMEE54 pKa = 5.69EE55 pKa = 4.53INTLQNQGAWPAGAGRR71 pKa = 11.84RR72 pKa = 11.84VGLSNPTPQEE82 pKa = 3.55IPQPQWTPEE91 pKa = 3.83EE92 pKa = 4.01DD93 pKa = 3.23QKK95 pKa = 11.68ARR97 pKa = 11.84EE98 pKa = 3.95AFRR101 pKa = 11.84RR102 pKa = 11.84YY103 pKa = 8.96QEE105 pKa = 3.91EE106 pKa = 4.36RR107 pKa = 11.84PPEE110 pKa = 4.17TTTIPPSSPPQWKK123 pKa = 8.78LQPGDD128 pKa = 4.58DD129 pKa = 4.21PLLGNQSLLEE139 pKa = 4.04THH141 pKa = 7.35PLYY144 pKa = 10.57QSEE147 pKa = 4.28PAVPVIKK154 pKa = 9.85TPPLKK159 pKa = 10.58KK160 pKa = 10.34KK161 pKa = 10.19MSGTFGGILAGLIGLLVSFFLLIKK185 pKa = 10.07ILEE188 pKa = 4.06ILRR191 pKa = 11.84RR192 pKa = 11.84LDD194 pKa = 3.1WWWISLSSPKK204 pKa = 10.95GKK206 pKa = 8.31MQCAFQDD213 pKa = 3.1TGAQISPHH221 pKa = 5.58YY222 pKa = 10.41VGSCPWGCPGFLWTYY237 pKa = 10.39LRR239 pKa = 11.84LFIIFLLILLVAAGLLYY256 pKa = 10.14LTDD259 pKa = 3.77NGSTILGKK267 pKa = 9.65LQWASVSALFSSISSLLPSDD287 pKa = 3.98PKK289 pKa = 11.3SLVALTFGLSLIWMTSSSATQTLVTLTQLATLSALFYY326 pKa = 10.91KK327 pKa = 10.61SS328 pKa = 2.99

Molecular weight: 36.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03145-2|HBSAG-2_DHBV1 Isoform of P03145 Isoform S of Large envelope protein OS=Duck hepatitis B virus (strain United States/DHBV-16) OX=489543 GN=S PE=1 SV=4
MM1 pKa = 7.55SGTFGGILAGLIGLLVSFFLLIKK24 pKa = 10.07ILEE27 pKa = 4.06ILRR30 pKa = 11.84RR31 pKa = 11.84LDD33 pKa = 3.1WWWISLSSPKK43 pKa = 10.95GKK45 pKa = 8.31MQCAFQDD52 pKa = 3.1TGAQISPHH60 pKa = 5.58YY61 pKa = 10.41VGSCPWGCPGFLWTYY76 pKa = 10.39LRR78 pKa = 11.84LFIIFLLILLVAAGLLYY95 pKa = 10.14LTDD98 pKa = 3.77NGSTILGKK106 pKa = 9.65LQWASVSALFSSISSLLPSDD126 pKa = 3.98PKK128 pKa = 11.3SLVALTFGLSLIWMTSSSATQTLVTLTQLATLSALFYY165 pKa = 10.91KK166 pKa = 10.61SS167 pKa = 2.99

Molecular weight: 18.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

1

2

495

167

328

247.5

27.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.061 ± 0.279

1.212 ± 0.31

2.828 ± 0.229

3.03 ± 1.288

4.444 ± 0.818

8.081 ± 0.16

1.212 ± 0.325

6.667 ± 0.592

4.242 ± 0.344

16.768 ± 1.903

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.02 ± 0.119

1.414 ± 0.432

6.869 ± 1.735

5.253 ± 0.879

3.03 ± 0.654

10.303 ± 1.521

7.273 ± 0.046

3.636 ± 0.023

3.636 ± 0.294

2.02 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski