Corchorus olitorius
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 35489 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R3JVW1|A0A1R3JVW1_9ROSI CCT-theta OS=Corchorus olitorius OX=93759 GN=COLO4_13606 PE=3 SV=1
MM1 pKa = 7.83 DD2 pKa = 4.74 NKK4 pKa = 10.24 NGSMVDD10 pKa = 3.32 VSCFLLVEE18 pKa = 4.48 DD19 pKa = 5.07 SGDD22 pKa = 3.95 SEE24 pKa = 5.86 LEE26 pKa = 3.8 RR27 pKa = 11.84 QGNMDD32 pKa = 4.88 IIATMAADD40 pKa = 3.84 NYY42 pKa = 10.26 HH43 pKa = 7.29 DD44 pKa = 4.35 EE45 pKa = 5.03 GDD47 pKa = 4.59 AEE49 pKa = 4.45 SCSCDD54 pKa = 3.04 TSEE57 pKa = 5.74 RR58 pKa = 11.84 LDD60 pKa = 3.67 DD61 pKa = 5.55 FYY63 pKa = 11.82 DD64 pKa = 3.72 EE65 pKa = 5.76 LVQLDD70 pKa = 3.88 DD71 pKa = 4.41 HH72 pKa = 7.08 HH73 pKa = 7.21 DD74 pKa = 3.47 QGFKK78 pKa = 10.36 DD79 pKa = 4.87 CKK81 pKa = 10.73 LLDD84 pKa = 4.83 PIAADD89 pKa = 3.68 GWSSCSKK96 pKa = 9.75 IWSIDD101 pKa = 3.35 EE102 pKa = 4.33 ALGSTLSVDD111 pKa = 3.91 DD112 pKa = 4.17 QEE114 pKa = 4.56 QSFGINLCRR123 pKa = 11.84 EE124 pKa = 4.24 VMDD127 pKa = 4.17 GMEE130 pKa = 5.78 DD131 pKa = 3.16 RR132 pKa = 11.84 LFWEE136 pKa = 4.15 TCIAVGYY143 pKa = 8.54 PLNN146 pKa = 3.93
Molecular weight: 16.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.681
IPC_protein 3.694
Toseland 3.465
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.516
Grimsley 3.376
Solomon 3.681
Lehninger 3.643
Nozaki 3.808
DTASelect 4.062
Thurlkill 3.528
EMBOSS 3.656
Sillero 3.821
Patrickios 1.062
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.734
Protein with the highest isoelectric point:
>tr|A0A1R3ILW5|A0A1R3ILW5_9ROSI Zonadhesin-like protein OS=Corchorus olitorius OX=93759 GN=COLO4_22410 PE=4 SV=1
MM1 pKa = 7.82 KK2 pKa = 10.29 ARR4 pKa = 11.84 NPPARR9 pKa = 11.84 RR10 pKa = 11.84 IPRR13 pKa = 11.84 LMRR16 pKa = 11.84 RR17 pKa = 11.84 KK18 pKa = 9.34 RR19 pKa = 11.84 HH20 pKa = 6.05 RR21 pKa = 11.84 GWPPRR26 pKa = 11.84 GWRR29 pKa = 11.84 STT31 pKa = 3.36
Molecular weight: 3.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.544
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.281
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.178
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
35489
0
35489
11105778
29
4461
312.9
34.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.83 ± 0.015
1.862 ± 0.008
5.293 ± 0.01
6.515 ± 0.015
4.285 ± 0.012
6.536 ± 0.012
2.337 ± 0.006
5.384 ± 0.011
6.2 ± 0.013
9.646 ± 0.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.471 ± 0.006
4.563 ± 0.011
4.985 ± 0.016
3.764 ± 0.011
5.291 ± 0.013
8.711 ± 0.018
4.8 ± 0.009
6.453 ± 0.01
1.305 ± 0.005
2.768 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here