Emticicia sp. TH156
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4502 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N4XDQ6|A0A2N4XDQ6_9BACT RNA polymerase subunit sigma OS=Emticicia sp. TH156 OX=2067454 GN=C0V77_08685 PE=4 SV=1
MM1 pKa = 7.11 ITPISFKK8 pKa = 10.81 NDD10 pKa = 2.74 LSYY13 pKa = 11.26 GVSIYY18 pKa = 10.91 LSTDD22 pKa = 3.08 DD23 pKa = 4.54 TNAGQPNYY31 pKa = 9.63 IARR34 pKa = 11.84 LSFVGHH40 pKa = 6.77 IEE42 pKa = 3.88 PAATLAISQQSDD54 pKa = 3.46 LTSVYY59 pKa = 10.11 ICYY62 pKa = 10.39 DD63 pKa = 3.54 NQNKK67 pKa = 8.48 PVKK70 pKa = 10.27 RR71 pKa = 11.84 MVYY74 pKa = 9.98 EE75 pKa = 4.73 GGSSQQGFTITQADD89 pKa = 3.69 ADD91 pKa = 4.47 VIASNYY97 pKa = 10.51 SFFAFIAANPNDD109 pKa = 3.94 ALSVSFAKK117 pKa = 9.36 LTEE120 pKa = 4.69 GGNATPDD127 pKa = 3.5 QIDD130 pKa = 3.2 AFYY133 pKa = 10.62 EE134 pKa = 4.42 GTANYY139 pKa = 7.83 QACTYY144 pKa = 10.41 VSYY147 pKa = 11.05 ILVLAALSADD157 pKa = 3.76 TGTSPADD164 pKa = 3.18 TAYY167 pKa = 10.97 SLKK170 pKa = 10.99 SLVGYY175 pKa = 10.44 LGVNWPGLVPDD186 pKa = 4.54 FPVRR190 pKa = 11.84 SFTCTTLNGNGNLVSLSCDD209 pKa = 2.92 VNMEE213 pKa = 4.79 DD214 pKa = 5.27 IYY216 pKa = 11.19 FGSQIAANIFTITPFNSVKK235 pKa = 10.09 VVHH238 pKa = 5.95 VNISFSYY245 pKa = 9.88 TLTPDD250 pKa = 3.56 LVTVDD255 pKa = 3.89 LTFSLDD261 pKa = 3.58 AIRR264 pKa = 11.84 IPVAANTDD272 pKa = 3.21 ITLSNPAITFSLSPQSGEE290 pKa = 3.9 VSFMAQATIPFKK302 pKa = 10.75 LWSNPTFYY310 pKa = 11.32 ADD312 pKa = 3.17 LSMVIGADD320 pKa = 3.21 QANIGVVIRR329 pKa = 11.84 GDD331 pKa = 3.33 NSVLFTPPIIQGVHH345 pKa = 4.4 FTSFGVGMGVFFQPIIGYY363 pKa = 8.65 SLGVQGSFTIGKK375 pKa = 9.03 PEE377 pKa = 4.6 DD378 pKa = 3.63 NVLVEE383 pKa = 4.91 DD384 pKa = 3.61 NSFAVVCALIGDD396 pKa = 4.57 IPNPLYY402 pKa = 10.36 ISFYY406 pKa = 10.49 VSQISLNEE414 pKa = 3.85 LVTIFTDD421 pKa = 2.95 VDD423 pKa = 4.1 FNIDD427 pKa = 3.52 FPVSVKK433 pKa = 10.52 NLSFVWQEE441 pKa = 3.84 NPTQPVVLPDD451 pKa = 3.89 GSTSKK456 pKa = 11.47 YY457 pKa = 9.18 EE458 pKa = 4.14 FGFSGYY464 pKa = 10.8 LDD466 pKa = 3.61 FFGLGFYY473 pKa = 11.01 GDD475 pKa = 4.88 LDD477 pKa = 4.01 INPVSGVNGIITMSPYY493 pKa = 10.8 EE494 pKa = 4.68 LGPLSLKK501 pKa = 11.04 GNGQAISIKK510 pKa = 10.32 VNEE513 pKa = 4.69 AGDD516 pKa = 4.42 PISNNVVPEE525 pKa = 4.31 TSAEE529 pKa = 3.85 EE530 pKa = 3.83 LAIEE534 pKa = 4.16 NAGTQVIIQAGGPVMEE550 pKa = 4.56 VSTFEE555 pKa = 3.84 SPYY558 pKa = 9.64 FVASIAIAFLDD569 pKa = 3.75 CRR571 pKa = 11.84 QSVEE575 pKa = 4.41 ASVTNNGFSFEE586 pKa = 4.76 LDD588 pKa = 3.64 FEE590 pKa = 4.54 ATLTSSMACVLKK602 pKa = 10.61 NYY604 pKa = 10.13 QYY606 pKa = 11.19 FSGSFTYY613 pKa = 10.44 GADD616 pKa = 3.3 LMIRR620 pKa = 11.84 IPDD623 pKa = 3.67 SVDD626 pKa = 3.0 VFNLGSIHH634 pKa = 6.24 FVSTISSEE642 pKa = 4.17 LTIDD646 pKa = 3.75 TSADD650 pKa = 3.57 VIRR653 pKa = 11.84 FTYY656 pKa = 10.42 AGSFDD661 pKa = 3.49 FAGYY665 pKa = 9.72 HH666 pKa = 4.15 YY667 pKa = 9.96 HH668 pKa = 7.53 IPEE671 pKa = 4.71 LSLDD675 pKa = 3.59 INTQSLQDD683 pKa = 4.01 IINTANQWIIDD694 pKa = 3.9 NVSTLFKK701 pKa = 10.77 NLYY704 pKa = 10.35 ADD706 pKa = 3.48 ATAWINAITQGVIVLATNTAQYY728 pKa = 10.34 VIEE731 pKa = 4.44 GFKK734 pKa = 10.67 VVYY737 pKa = 9.77 GVSAEE742 pKa = 4.11 AVGDD746 pKa = 3.86 LLKK749 pKa = 10.3 GTNYY753 pKa = 10.96 ALNDD757 pKa = 3.39 VAKK760 pKa = 10.58 GMKK763 pKa = 9.58 DD764 pKa = 3.29 VYY766 pKa = 10.92 NATSTLTTQTLVTAYY781 pKa = 10.54 NAAEE785 pKa = 4.4 DD786 pKa = 3.82 SAAAALQYY794 pKa = 11.11 AGYY797 pKa = 7.64 TADD800 pKa = 4.62 EE801 pKa = 4.32 IADD804 pKa = 3.74 ALVNVYY810 pKa = 10.12 GSTMEE815 pKa = 4.07 VVGEE819 pKa = 3.82 ILLALGYY826 pKa = 10.1 SADD829 pKa = 3.66 VVAQALTDD837 pKa = 3.59 AFNATEE843 pKa = 4.27 AEE845 pKa = 4.56 VAAVMAEE852 pKa = 4.17 LGYY855 pKa = 11.02 AIDD858 pKa = 3.77 TAGNWVEE865 pKa = 4.41 KK866 pKa = 8.29 TAKK869 pKa = 10.24 DD870 pKa = 3.61 AADD873 pKa = 3.59 ATKK876 pKa = 10.77 NAADD880 pKa = 4.53 DD881 pKa = 4.25 ALKK884 pKa = 10.56 QATDD888 pKa = 3.53 STKK891 pKa = 10.95 DD892 pKa = 3.37 ATDD895 pKa = 3.36 SAIKK899 pKa = 8.92 EE900 pKa = 4.48 TEE902 pKa = 4.06 DD903 pKa = 3.5 AAKK906 pKa = 10.53 DD907 pKa = 3.47 AADD910 pKa = 3.93 EE911 pKa = 4.33 AEE913 pKa = 4.54 KK914 pKa = 10.54 AAKK917 pKa = 9.99 DD918 pKa = 3.4 AADD921 pKa = 3.82 AAKK924 pKa = 10.49 DD925 pKa = 3.62 AADD928 pKa = 3.78 SALKK932 pKa = 10.56 ASEE935 pKa = 4.74 DD936 pKa = 3.79 AAKK939 pKa = 10.57 DD940 pKa = 3.35 AADD943 pKa = 3.86 AAKK946 pKa = 10.55 DD947 pKa = 3.67 AADD950 pKa = 4.01 EE951 pKa = 4.07 AAKK954 pKa = 10.15 AAKK957 pKa = 9.96 DD958 pKa = 3.34 AADD961 pKa = 3.65 AAKK964 pKa = 10.53 NATDD968 pKa = 3.57 SAKK971 pKa = 10.68 NATDD975 pKa = 3.53 SALKK979 pKa = 10.31 SAADD983 pKa = 4.18 AIIPP987 pKa = 3.89
Molecular weight: 104.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.859
IPC_protein 3.91
Toseland 3.681
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.719
Grimsley 3.579
Solomon 3.897
Lehninger 3.846
Nozaki 3.999
DTASelect 4.266
Thurlkill 3.719
EMBOSS 3.834
Sillero 4.024
Patrickios 1.214
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.907
Protein with the highest isoelectric point:
>tr|A0A2N4X6Q9|A0A2N4X6Q9_9BACT TIR domain-containing protein OS=Emticicia sp. TH156 OX=2067454 GN=C0V77_22715 PE=4 SV=1
MM1 pKa = 7.92 RR2 pKa = 11.84 ILRR5 pKa = 11.84 FTLFFFIAGLLIAPQVQATHH25 pKa = 6.19 LRR27 pKa = 11.84 AGEE30 pKa = 3.92 ITAKK34 pKa = 10.22 RR35 pKa = 11.84 ISSTTLTYY43 pKa = 10.55 RR44 pKa = 11.84 VTLTTYY50 pKa = 9.08 TDD52 pKa = 3.46 QINGYY57 pKa = 8.6 IANDD61 pKa = 3.62 AANTSQFSFGVTGVPLFEE79 pKa = 4.15 VKK81 pKa = 9.8 RR82 pKa = 11.84 RR83 pKa = 11.84 KK84 pKa = 9.91 KK85 pKa = 10.62 FLINSS90 pKa = 3.94
Molecular weight: 10.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.984
IPC_protein 10.833
Toseland 10.804
ProMoST 10.555
Dawson 10.906
Bjellqvist 10.643
Wikipedia 11.14
Rodwell 11.125
Grimsley 10.965
Solomon 11.038
Lehninger 11.008
Nozaki 10.774
DTASelect 10.643
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.833
Patrickios 10.906
IPC_peptide 11.038
IPC2_peptide 9.531
IPC2.peptide.svr19 8.301
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4502
0
4502
1548216
25
2417
343.9
38.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.435 ± 0.037
0.775 ± 0.011
5.155 ± 0.024
6.077 ± 0.041
5.089 ± 0.032
6.764 ± 0.035
1.791 ± 0.017
7.226 ± 0.035
7.186 ± 0.032
9.508 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.338 ± 0.018
5.51 ± 0.032
3.866 ± 0.021
3.726 ± 0.023
4.149 ± 0.024
6.108 ± 0.025
5.655 ± 0.026
6.377 ± 0.026
1.225 ± 0.015
4.041 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here