Allium cepa amalgavirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; Amalgavirus

Average proteome isoelectric point is 8.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2H5MI89|A0A2H5MI89_9VIRU ORF1+2p OS=Allium cepa amalgavirus 1 OX=2058778 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.2SAEE4 pKa = 4.36DD5 pKa = 3.62FTPRR9 pKa = 11.84TGLVDD14 pKa = 3.47TEE16 pKa = 4.67LLVDD20 pKa = 4.16PAAEE24 pKa = 4.17LEE26 pKa = 4.19EE27 pKa = 4.3LHH29 pKa = 6.95DD30 pKa = 4.4ALMPLEE36 pKa = 3.97VLNINVKK43 pKa = 10.51AFTRR47 pKa = 11.84ANIFALRR54 pKa = 11.84MTVPQYY60 pKa = 10.2IKK62 pKa = 10.34EE63 pKa = 4.11VRR65 pKa = 11.84VLQNLQDD72 pKa = 3.46VQLIRR77 pKa = 11.84KK78 pKa = 7.73VWTEE82 pKa = 4.16AISHH86 pKa = 6.23KK87 pKa = 10.57CATLPDD93 pKa = 3.72EE94 pKa = 5.3ADD96 pKa = 3.38AGVCLSFARR105 pKa = 11.84WLKK108 pKa = 10.28RR109 pKa = 11.84GVGAKK114 pKa = 9.72VLSDD118 pKa = 3.41AQHH121 pKa = 7.03LIKK124 pKa = 10.47LQKK127 pKa = 9.77RR128 pKa = 11.84AVGSVEE134 pKa = 3.98PKK136 pKa = 10.37VLAFVQLLDD145 pKa = 3.54QQIADD150 pKa = 3.41MHH152 pKa = 6.61AEE154 pKa = 3.97RR155 pKa = 11.84KK156 pKa = 9.6KK157 pKa = 10.57IQAEE161 pKa = 4.31GQAKK165 pKa = 9.25IDD167 pKa = 3.5EE168 pKa = 4.57LRR170 pKa = 11.84RR171 pKa = 11.84EE172 pKa = 3.99IQRR175 pKa = 11.84VEE177 pKa = 3.68RR178 pKa = 11.84EE179 pKa = 3.71YY180 pKa = 11.37DD181 pKa = 3.49EE182 pKa = 4.47KK183 pKa = 11.52AKK185 pKa = 10.86ASSKK189 pKa = 9.92RR190 pKa = 11.84FKK192 pKa = 10.15PARR195 pKa = 11.84KK196 pKa = 9.02YY197 pKa = 10.82VPPTKK202 pKa = 10.62AKK204 pKa = 9.87IDD206 pKa = 3.83EE207 pKa = 4.6EE208 pKa = 4.39CWNAYY213 pKa = 8.88LNKK216 pKa = 9.32VQKK219 pKa = 10.16SGKK222 pKa = 7.22TAPEE226 pKa = 3.73WNAVLQEE233 pKa = 4.23QANTMYY239 pKa = 10.32QQLYY243 pKa = 8.14LTQHH247 pKa = 5.94KK248 pKa = 10.46QDD250 pKa = 3.44FCGLEE255 pKa = 4.21SNQIPLKK262 pKa = 10.33VWADD266 pKa = 3.5SKK268 pKa = 11.37LKK270 pKa = 10.45EE271 pKa = 4.19LADD274 pKa = 3.36NHH276 pKa = 6.15EE277 pKa = 4.96FVASARR283 pKa = 11.84PSEE286 pKa = 4.35SWVSRR291 pKa = 11.84VEE293 pKa = 4.17SHH295 pKa = 6.7LHH297 pKa = 6.29RR298 pKa = 11.84LPLPQRR304 pKa = 11.84VFQANNVPVGPVSQLVMNQSRR325 pKa = 11.84NMALRR330 pKa = 11.84TLLNPQLLTWKK341 pKa = 9.9SQIGMRR347 pKa = 11.84KK348 pKa = 9.42KK349 pKa = 10.56PPLQKK354 pKa = 10.72ANEE357 pKa = 4.19SRR359 pKa = 11.84SSKK362 pKa = 10.23IDD364 pKa = 3.46LLLRR368 pKa = 11.84NPRR371 pKa = 11.84ILVLRR376 pKa = 11.84KK377 pKa = 9.39PEE379 pKa = 4.29RR380 pKa = 11.84GPLGGIPTARR390 pKa = 11.84SKK392 pKa = 11.21FEE394 pKa = 3.76AMVRR398 pKa = 11.84KK399 pKa = 10.0VIGGGEE405 pKa = 4.02MLNWSIDD412 pKa = 3.41SNMYY416 pKa = 10.3RR417 pKa = 11.84GGGNFTDD424 pKa = 4.67ALTLLADD431 pKa = 3.8ARR433 pKa = 11.84YY434 pKa = 8.4DD435 pKa = 3.55APEE438 pKa = 4.26MFLSDD443 pKa = 3.6YY444 pKa = 11.35LSIEE448 pKa = 4.09KK449 pKa = 10.34ARR451 pKa = 11.84SLLCLPSDD459 pKa = 4.32LKK461 pKa = 11.38VPCHH465 pKa = 6.22RR466 pKa = 11.84NCVSVNNFNNEE477 pKa = 3.37ATAGPFFRR485 pKa = 11.84AHH487 pKa = 7.73GIRR490 pKa = 11.84NKK492 pKa = 10.74YY493 pKa = 9.14GMRR496 pKa = 11.84LQLEE500 pKa = 4.23DD501 pKa = 4.92FAWEE505 pKa = 4.55CYY507 pKa = 8.84NAYY510 pKa = 9.97VDD512 pKa = 4.35SGGNPSMLPYY522 pKa = 10.06ISSRR526 pKa = 11.84VGFRR530 pKa = 11.84TKK532 pKa = 10.53LVSTTEE538 pKa = 4.01AFAKK542 pKa = 10.25MKK544 pKa = 10.26DD545 pKa = 3.32NKK547 pKa = 10.54PIGRR551 pKa = 11.84CVMMLDD557 pKa = 4.91AIEE560 pKa = 4.41QMFSTPLYY568 pKa = 10.03NVLSKK573 pKa = 8.07TTAAQRR579 pKa = 11.84FDD581 pKa = 3.23IKK583 pKa = 11.04SGFRR587 pKa = 11.84NTIVRR592 pKa = 11.84ASSDD596 pKa = 2.73WAKK599 pKa = 10.46FWEE602 pKa = 4.42EE603 pKa = 3.56VKK605 pKa = 10.31AAKK608 pKa = 10.42VIVEE612 pKa = 4.28LDD614 pKa = 2.88WKK616 pKa = 10.85KK617 pKa = 10.69FDD619 pKa = 4.23RR620 pKa = 11.84EE621 pKa = 4.11RR622 pKa = 11.84PTEE625 pKa = 4.05DD626 pKa = 3.74LEE628 pKa = 5.5FIIQVIISCFKK639 pKa = 9.55PTDD642 pKa = 3.27EE643 pKa = 5.36RR644 pKa = 11.84EE645 pKa = 3.89RR646 pKa = 11.84LFLRR650 pKa = 11.84GYY652 pKa = 10.81GIMLRR657 pKa = 11.84RR658 pKa = 11.84CLIDD662 pKa = 4.24RR663 pKa = 11.84YY664 pKa = 10.44FITDD668 pKa = 3.36DD669 pKa = 3.69GGVFKK674 pKa = 10.44IDD676 pKa = 3.25GMVPSGSLWTGWVDD690 pKa = 3.2TALNILYY697 pKa = 8.69LQSVMISLGLVSTAVSPKK715 pKa = 10.4CAGDD719 pKa = 4.0DD720 pKa = 3.7NLTLFWKK727 pKa = 10.68DD728 pKa = 3.18FDD730 pKa = 4.59DD731 pKa = 5.82KK732 pKa = 11.31RR733 pKa = 11.84LLSIKK738 pKa = 9.31TRR740 pKa = 11.84LNAWFRR746 pKa = 11.84AGIDD750 pKa = 3.69DD751 pKa = 4.04EE752 pKa = 5.82DD753 pKa = 4.81FFIHH757 pKa = 6.78RR758 pKa = 11.84PPYY761 pKa = 9.97HH762 pKa = 5.2VTRR765 pKa = 11.84EE766 pKa = 4.01QATFPPGTDD775 pKa = 3.16LTKK778 pKa = 10.06GTSRR782 pKa = 11.84KK783 pKa = 9.96LKK785 pKa = 9.73DD786 pKa = 4.7AIWIPIDD793 pKa = 3.61GEE795 pKa = 4.34PIIDD799 pKa = 3.55QAQGLSHH806 pKa = 6.84RR807 pKa = 11.84WQYY810 pKa = 9.82VFRR813 pKa = 11.84GKK815 pKa = 9.77PKK817 pKa = 10.19FLSAYY822 pKa = 7.8WLEE825 pKa = 4.52DD826 pKa = 3.06GRR828 pKa = 11.84PIRR831 pKa = 11.84PTHH834 pKa = 5.8VNAEE838 pKa = 4.07KK839 pKa = 10.92LLFPEE844 pKa = 6.28GIHH847 pKa = 7.16KK848 pKa = 10.51DD849 pKa = 3.04IEE851 pKa = 4.43QYY853 pKa = 10.82EE854 pKa = 4.52SAVLSMIVDD863 pKa = 3.64NPYY866 pKa = 9.65NHH868 pKa = 7.02HH869 pKa = 6.21NVNHH873 pKa = 6.0CMHH876 pKa = 6.99RR877 pKa = 11.84FIICEE882 pKa = 3.71QVKK885 pKa = 9.35RR886 pKa = 11.84QSAAGIDD893 pKa = 3.71PVDD896 pKa = 3.48ILWFSRR902 pKa = 11.84IRR904 pKa = 11.84PGNPDD909 pKa = 3.39VVPYY913 pKa = 10.6PMVASWRR920 pKa = 11.84RR921 pKa = 11.84CEE923 pKa = 4.44GYY925 pKa = 10.74VDD927 pKa = 5.38LEE929 pKa = 3.95QLPFISDD936 pKa = 3.53YY937 pKa = 11.71VKK939 pKa = 10.7DD940 pKa = 3.68LKK942 pKa = 11.18EE943 pKa = 3.96FVAGVTSLYY952 pKa = 10.99ARR954 pKa = 11.84DD955 pKa = 4.0SIGGLDD961 pKa = 2.97AWRR964 pKa = 11.84FTEE967 pKa = 4.72IIRR970 pKa = 11.84GEE972 pKa = 4.15TDD974 pKa = 2.8LGTGQFGNDD983 pKa = 2.52IDD985 pKa = 4.86VWIHH989 pKa = 5.62WLHH992 pKa = 5.91HH993 pKa = 6.38HH994 pKa = 7.24PLTKK998 pKa = 9.92YY999 pKa = 9.33LKK1001 pKa = 8.78PIKK1004 pKa = 9.86RR1005 pKa = 11.84HH1006 pKa = 4.96RR1007 pKa = 11.84QPPLSVMPDD1016 pKa = 3.22KK1017 pKa = 10.46EE1018 pKa = 3.91LHH1020 pKa = 5.53GRR1022 pKa = 11.84IEE1024 pKa = 4.04EE1025 pKa = 4.14ALQVYY1030 pKa = 10.12RR1031 pKa = 11.84DD1032 pKa = 3.53IRR1034 pKa = 11.84GTTTNASAEE1043 pKa = 4.11AFSLFLSNVIKK1054 pKa = 10.55HH1055 pKa = 5.57QGG1057 pKa = 2.91

Molecular weight:
120.78 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2H5MI89|A0A2H5MI89_9VIRU ORF1+2p OS=Allium cepa amalgavirus 1 OX=2058778 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.2SAEE4 pKa = 4.36DD5 pKa = 3.62FTPRR9 pKa = 11.84TGLVDD14 pKa = 3.47TEE16 pKa = 4.67LLVDD20 pKa = 4.16PAAEE24 pKa = 4.17LEE26 pKa = 4.19EE27 pKa = 4.3LHH29 pKa = 6.95DD30 pKa = 4.4ALMPLEE36 pKa = 3.97VLNINVKK43 pKa = 10.51AFTRR47 pKa = 11.84ANIFALRR54 pKa = 11.84MTVPQYY60 pKa = 10.2IKK62 pKa = 10.34EE63 pKa = 4.11VRR65 pKa = 11.84VLQNLQDD72 pKa = 3.46VQLIRR77 pKa = 11.84KK78 pKa = 7.73VWTEE82 pKa = 4.16AISHH86 pKa = 6.23KK87 pKa = 10.57CATLPDD93 pKa = 3.72EE94 pKa = 5.3ADD96 pKa = 3.38AGVCLSFARR105 pKa = 11.84WLKK108 pKa = 10.28RR109 pKa = 11.84GVGAKK114 pKa = 9.72VLSDD118 pKa = 3.41AQHH121 pKa = 7.03LIKK124 pKa = 10.47LQKK127 pKa = 9.77RR128 pKa = 11.84AVGSVEE134 pKa = 3.98PKK136 pKa = 10.37VLAFVQLLDD145 pKa = 3.54QQIADD150 pKa = 3.41MHH152 pKa = 6.61AEE154 pKa = 3.97RR155 pKa = 11.84KK156 pKa = 9.6KK157 pKa = 10.57IQAEE161 pKa = 4.31GQAKK165 pKa = 9.25IDD167 pKa = 3.5EE168 pKa = 4.57LRR170 pKa = 11.84RR171 pKa = 11.84EE172 pKa = 3.99IQRR175 pKa = 11.84VEE177 pKa = 3.68RR178 pKa = 11.84EE179 pKa = 3.71YY180 pKa = 11.37DD181 pKa = 3.49EE182 pKa = 4.47KK183 pKa = 11.52AKK185 pKa = 10.86ASSKK189 pKa = 9.92RR190 pKa = 11.84FKK192 pKa = 10.15PARR195 pKa = 11.84KK196 pKa = 9.02YY197 pKa = 10.82VPPTKK202 pKa = 10.62AKK204 pKa = 9.87IDD206 pKa = 3.83EE207 pKa = 4.6EE208 pKa = 4.39CWNAYY213 pKa = 8.88LNKK216 pKa = 9.32VQKK219 pKa = 10.16SGKK222 pKa = 7.22TAPEE226 pKa = 3.73WNAVLQEE233 pKa = 4.23QANTMYY239 pKa = 10.32QQLYY243 pKa = 8.14LTQHH247 pKa = 5.94KK248 pKa = 10.46QDD250 pKa = 3.44FCGLEE255 pKa = 4.21SNQIPLKK262 pKa = 10.33VWADD266 pKa = 3.5SKK268 pKa = 11.37LKK270 pKa = 10.45EE271 pKa = 4.19LADD274 pKa = 3.27NHH276 pKa = 5.11EE277 pKa = 4.24FRR279 pKa = 11.84RR280 pKa = 11.84VRR282 pKa = 11.84EE283 pKa = 3.95TQRR286 pKa = 11.84ILGLASGVPSSPSASASKK304 pKa = 10.59SLSGKK309 pKa = 8.42QRR311 pKa = 11.84SGGPSQSAGDD321 pKa = 3.94EE322 pKa = 4.08PVEE325 pKa = 4.05EE326 pKa = 4.82HH327 pKa = 6.76GSSNSAKK334 pKa = 9.86PAAPDD339 pKa = 3.38MEE341 pKa = 4.2ITDD344 pKa = 3.81RR345 pKa = 11.84DD346 pKa = 3.76EE347 pKa = 4.72EE348 pKa = 4.41KK349 pKa = 10.35TSSSKK354 pKa = 10.79GKK356 pKa = 10.2RR357 pKa = 11.84KK358 pKa = 9.44QKK360 pKa = 10.84LKK362 pKa = 11.05DD363 pKa = 3.04RR364 pKa = 11.84LAAKK368 pKa = 9.99KK369 pKa = 10.78SKK371 pKa = 10.46DD372 pKa = 3.31LSAKK376 pKa = 8.59KK377 pKa = 8.27TRR379 pKa = 11.84KK380 pKa = 7.94GTIRR384 pKa = 11.84RR385 pKa = 11.84DD386 pKa = 3.39PHH388 pKa = 6.13RR389 pKa = 11.84KK390 pKa = 8.93KK391 pKa = 10.85

Molecular weight:
43.97 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1448

391

1057

724.0

82.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.08 ± 1.134

1.312 ± 0.152

6.146 ± 0.139

6.492 ± 0.758

3.66 ± 0.851

5.11 ± 0.402

2.348 ± 0.159

5.387 ± 0.817

7.735 ± 1.72

9.599 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.141 ± 0.32

3.729 ± 0.483

5.18 ± 0.304

4.696 ± 0.76

6.975 ± 0.037

6.285 ± 0.597

4.282 ± 0.1

6.492 ± 0.186

2.003 ± 0.382

2.348 ± 0.429

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski