Allium cepa amalgavirus 1
Average proteome isoelectric point is 8.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5MI89|A0A2H5MI89_9VIRU ORF1+2p OS=Allium cepa amalgavirus 1 OX=2058778 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.2 SAEE4 pKa = 4.36 DD5 pKa = 3.62 FTPRR9 pKa = 11.84 TGLVDD14 pKa = 3.47 TEE16 pKa = 4.67 LLVDD20 pKa = 4.16 PAAEE24 pKa = 4.17 LEE26 pKa = 4.19 EE27 pKa = 4.3 LHH29 pKa = 6.95 DD30 pKa = 4.4 ALMPLEE36 pKa = 3.97 VLNINVKK43 pKa = 10.51 AFTRR47 pKa = 11.84 ANIFALRR54 pKa = 11.84 MTVPQYY60 pKa = 10.2 IKK62 pKa = 10.34 EE63 pKa = 4.11 VRR65 pKa = 11.84 VLQNLQDD72 pKa = 3.46 VQLIRR77 pKa = 11.84 KK78 pKa = 7.73 VWTEE82 pKa = 4.16 AISHH86 pKa = 6.23 KK87 pKa = 10.57 CATLPDD93 pKa = 3.72 EE94 pKa = 5.3 ADD96 pKa = 3.38 AGVCLSFARR105 pKa = 11.84 WLKK108 pKa = 10.28 RR109 pKa = 11.84 GVGAKK114 pKa = 9.72 VLSDD118 pKa = 3.41 AQHH121 pKa = 7.03 LIKK124 pKa = 10.47 LQKK127 pKa = 9.77 RR128 pKa = 11.84 AVGSVEE134 pKa = 3.98 PKK136 pKa = 10.37 VLAFVQLLDD145 pKa = 3.54 QQIADD150 pKa = 3.41 MHH152 pKa = 6.61 AEE154 pKa = 3.97 RR155 pKa = 11.84 KK156 pKa = 9.6 KK157 pKa = 10.57 IQAEE161 pKa = 4.31 GQAKK165 pKa = 9.25 IDD167 pKa = 3.5 EE168 pKa = 4.57 LRR170 pKa = 11.84 RR171 pKa = 11.84 EE172 pKa = 3.99 IQRR175 pKa = 11.84 VEE177 pKa = 3.68 RR178 pKa = 11.84 EE179 pKa = 3.71 YY180 pKa = 11.37 DD181 pKa = 3.49 EE182 pKa = 4.47 KK183 pKa = 11.52 AKK185 pKa = 10.86 ASSKK189 pKa = 9.92 RR190 pKa = 11.84 FKK192 pKa = 10.15 PARR195 pKa = 11.84 KK196 pKa = 9.02 YY197 pKa = 10.82 VPPTKK202 pKa = 10.62 AKK204 pKa = 9.87 IDD206 pKa = 3.83 EE207 pKa = 4.6 EE208 pKa = 4.39 CWNAYY213 pKa = 8.88 LNKK216 pKa = 9.32 VQKK219 pKa = 10.16 SGKK222 pKa = 7.22 TAPEE226 pKa = 3.73 WNAVLQEE233 pKa = 4.23 QANTMYY239 pKa = 10.32 QQLYY243 pKa = 8.14 LTQHH247 pKa = 5.94 KK248 pKa = 10.46 QDD250 pKa = 3.44 FCGLEE255 pKa = 4.21 SNQIPLKK262 pKa = 10.33 VWADD266 pKa = 3.5 SKK268 pKa = 11.37 LKK270 pKa = 10.45 EE271 pKa = 4.19 LADD274 pKa = 3.36 NHH276 pKa = 6.15 EE277 pKa = 4.96 FVASARR283 pKa = 11.84 PSEE286 pKa = 4.35 SWVSRR291 pKa = 11.84 VEE293 pKa = 4.17 SHH295 pKa = 6.7 LHH297 pKa = 6.29 RR298 pKa = 11.84 LPLPQRR304 pKa = 11.84 VFQANNVPVGPVSQLVMNQSRR325 pKa = 11.84 NMALRR330 pKa = 11.84 TLLNPQLLTWKK341 pKa = 9.9 SQIGMRR347 pKa = 11.84 KK348 pKa = 9.42 KK349 pKa = 10.56 PPLQKK354 pKa = 10.72 ANEE357 pKa = 4.19 SRR359 pKa = 11.84 SSKK362 pKa = 10.23 IDD364 pKa = 3.46 LLLRR368 pKa = 11.84 NPRR371 pKa = 11.84 ILVLRR376 pKa = 11.84 KK377 pKa = 9.39 PEE379 pKa = 4.29 RR380 pKa = 11.84 GPLGGIPTARR390 pKa = 11.84 SKK392 pKa = 11.21 FEE394 pKa = 3.76 AMVRR398 pKa = 11.84 KK399 pKa = 10.0 VIGGGEE405 pKa = 4.02 MLNWSIDD412 pKa = 3.41 SNMYY416 pKa = 10.3 RR417 pKa = 11.84 GGGNFTDD424 pKa = 4.67 ALTLLADD431 pKa = 3.8 ARR433 pKa = 11.84 YY434 pKa = 8.4 DD435 pKa = 3.55 APEE438 pKa = 4.26 MFLSDD443 pKa = 3.6 YY444 pKa = 11.35 LSIEE448 pKa = 4.09 KK449 pKa = 10.34 ARR451 pKa = 11.84 SLLCLPSDD459 pKa = 4.32 LKK461 pKa = 11.38 VPCHH465 pKa = 6.22 RR466 pKa = 11.84 NCVSVNNFNNEE477 pKa = 3.37 ATAGPFFRR485 pKa = 11.84 AHH487 pKa = 7.73 GIRR490 pKa = 11.84 NKK492 pKa = 10.74 YY493 pKa = 9.14 GMRR496 pKa = 11.84 LQLEE500 pKa = 4.23 DD501 pKa = 4.92 FAWEE505 pKa = 4.55 CYY507 pKa = 8.84 NAYY510 pKa = 9.97 VDD512 pKa = 4.35 SGGNPSMLPYY522 pKa = 10.06 ISSRR526 pKa = 11.84 VGFRR530 pKa = 11.84 TKK532 pKa = 10.53 LVSTTEE538 pKa = 4.01 AFAKK542 pKa = 10.25 MKK544 pKa = 10.26 DD545 pKa = 3.32 NKK547 pKa = 10.54 PIGRR551 pKa = 11.84 CVMMLDD557 pKa = 4.91 AIEE560 pKa = 4.41 QMFSTPLYY568 pKa = 10.03 NVLSKK573 pKa = 8.07 TTAAQRR579 pKa = 11.84 FDD581 pKa = 3.23 IKK583 pKa = 11.04 SGFRR587 pKa = 11.84 NTIVRR592 pKa = 11.84 ASSDD596 pKa = 2.73 WAKK599 pKa = 10.46 FWEE602 pKa = 4.42 EE603 pKa = 3.56 VKK605 pKa = 10.31 AAKK608 pKa = 10.42 VIVEE612 pKa = 4.28 LDD614 pKa = 2.88 WKK616 pKa = 10.85 KK617 pKa = 10.69 FDD619 pKa = 4.23 RR620 pKa = 11.84 EE621 pKa = 4.11 RR622 pKa = 11.84 PTEE625 pKa = 4.05 DD626 pKa = 3.74 LEE628 pKa = 5.5 FIIQVIISCFKK639 pKa = 9.55 PTDD642 pKa = 3.27 EE643 pKa = 5.36 RR644 pKa = 11.84 EE645 pKa = 3.89 RR646 pKa = 11.84 LFLRR650 pKa = 11.84 GYY652 pKa = 10.81 GIMLRR657 pKa = 11.84 RR658 pKa = 11.84 CLIDD662 pKa = 4.24 RR663 pKa = 11.84 YY664 pKa = 10.44 FITDD668 pKa = 3.36 DD669 pKa = 3.69 GGVFKK674 pKa = 10.44 IDD676 pKa = 3.25 GMVPSGSLWTGWVDD690 pKa = 3.2 TALNILYY697 pKa = 8.69 LQSVMISLGLVSTAVSPKK715 pKa = 10.4 CAGDD719 pKa = 4.0 DD720 pKa = 3.7 NLTLFWKK727 pKa = 10.68 DD728 pKa = 3.18 FDD730 pKa = 4.59 DD731 pKa = 5.82 KK732 pKa = 11.31 RR733 pKa = 11.84 LLSIKK738 pKa = 9.31 TRR740 pKa = 11.84 LNAWFRR746 pKa = 11.84 AGIDD750 pKa = 3.69 DD751 pKa = 4.04 EE752 pKa = 5.82 DD753 pKa = 4.81 FFIHH757 pKa = 6.78 RR758 pKa = 11.84 PPYY761 pKa = 9.97 HH762 pKa = 5.2 VTRR765 pKa = 11.84 EE766 pKa = 4.01 QATFPPGTDD775 pKa = 3.16 LTKK778 pKa = 10.06 GTSRR782 pKa = 11.84 KK783 pKa = 9.96 LKK785 pKa = 9.73 DD786 pKa = 4.7 AIWIPIDD793 pKa = 3.61 GEE795 pKa = 4.34 PIIDD799 pKa = 3.55 QAQGLSHH806 pKa = 6.84 RR807 pKa = 11.84 WQYY810 pKa = 9.82 VFRR813 pKa = 11.84 GKK815 pKa = 9.77 PKK817 pKa = 10.19 FLSAYY822 pKa = 7.8 WLEE825 pKa = 4.52 DD826 pKa = 3.06 GRR828 pKa = 11.84 PIRR831 pKa = 11.84 PTHH834 pKa = 5.8 VNAEE838 pKa = 4.07 KK839 pKa = 10.92 LLFPEE844 pKa = 6.28 GIHH847 pKa = 7.16 KK848 pKa = 10.51 DD849 pKa = 3.04 IEE851 pKa = 4.43 QYY853 pKa = 10.82 EE854 pKa = 4.52 SAVLSMIVDD863 pKa = 3.64 NPYY866 pKa = 9.65 NHH868 pKa = 7.02 HH869 pKa = 6.21 NVNHH873 pKa = 6.0 CMHH876 pKa = 6.99 RR877 pKa = 11.84 FIICEE882 pKa = 3.71 QVKK885 pKa = 9.35 RR886 pKa = 11.84 QSAAGIDD893 pKa = 3.71 PVDD896 pKa = 3.48 ILWFSRR902 pKa = 11.84 IRR904 pKa = 11.84 PGNPDD909 pKa = 3.39 VVPYY913 pKa = 10.6 PMVASWRR920 pKa = 11.84 RR921 pKa = 11.84 CEE923 pKa = 4.44 GYY925 pKa = 10.74 VDD927 pKa = 5.38 LEE929 pKa = 3.95 QLPFISDD936 pKa = 3.53 YY937 pKa = 11.71 VKK939 pKa = 10.7 DD940 pKa = 3.68 LKK942 pKa = 11.18 EE943 pKa = 3.96 FVAGVTSLYY952 pKa = 10.99 ARR954 pKa = 11.84 DD955 pKa = 4.0 SIGGLDD961 pKa = 2.97 AWRR964 pKa = 11.84 FTEE967 pKa = 4.72 IIRR970 pKa = 11.84 GEE972 pKa = 4.15 TDD974 pKa = 2.8 LGTGQFGNDD983 pKa = 2.52 IDD985 pKa = 4.86 VWIHH989 pKa = 5.62 WLHH992 pKa = 5.91 HH993 pKa = 6.38 HH994 pKa = 7.24 PLTKK998 pKa = 9.92 YY999 pKa = 9.33 LKK1001 pKa = 8.78 PIKK1004 pKa = 9.86 RR1005 pKa = 11.84 HH1006 pKa = 4.96 RR1007 pKa = 11.84 QPPLSVMPDD1016 pKa = 3.22 KK1017 pKa = 10.46 EE1018 pKa = 3.91 LHH1020 pKa = 5.53 GRR1022 pKa = 11.84 IEE1024 pKa = 4.04 EE1025 pKa = 4.14 ALQVYY1030 pKa = 10.12 RR1031 pKa = 11.84 DD1032 pKa = 3.53 IRR1034 pKa = 11.84 GTTTNASAEE1043 pKa = 4.11 AFSLFLSNVIKK1054 pKa = 10.55 HH1055 pKa = 5.57 QGG1057 pKa = 2.91
Molecular weight: 120.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.782
IPC2_protein 8.024
IPC_protein 7.849
Toseland 7.922
ProMoST 8.463
Dawson 8.726
Bjellqvist 8.873
Wikipedia 8.726
Rodwell 8.829
Grimsley 7.98
Solomon 8.785
Lehninger 8.77
Nozaki 9.048
DTASelect 8.668
Thurlkill 8.77
EMBOSS 8.916
Sillero 9.019
Patrickios 4.736
IPC_peptide 8.785
IPC2_peptide 7.585
IPC2.peptide.svr19 7.761
Protein with the highest isoelectric point:
>tr|A0A2H5MI89|A0A2H5MI89_9VIRU ORF1+2p OS=Allium cepa amalgavirus 1 OX=2058778 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.2 SAEE4 pKa = 4.36 DD5 pKa = 3.62 FTPRR9 pKa = 11.84 TGLVDD14 pKa = 3.47 TEE16 pKa = 4.67 LLVDD20 pKa = 4.16 PAAEE24 pKa = 4.17 LEE26 pKa = 4.19 EE27 pKa = 4.3 LHH29 pKa = 6.95 DD30 pKa = 4.4 ALMPLEE36 pKa = 3.97 VLNINVKK43 pKa = 10.51 AFTRR47 pKa = 11.84 ANIFALRR54 pKa = 11.84 MTVPQYY60 pKa = 10.2 IKK62 pKa = 10.34 EE63 pKa = 4.11 VRR65 pKa = 11.84 VLQNLQDD72 pKa = 3.46 VQLIRR77 pKa = 11.84 KK78 pKa = 7.73 VWTEE82 pKa = 4.16 AISHH86 pKa = 6.23 KK87 pKa = 10.57 CATLPDD93 pKa = 3.72 EE94 pKa = 5.3 ADD96 pKa = 3.38 AGVCLSFARR105 pKa = 11.84 WLKK108 pKa = 10.28 RR109 pKa = 11.84 GVGAKK114 pKa = 9.72 VLSDD118 pKa = 3.41 AQHH121 pKa = 7.03 LIKK124 pKa = 10.47 LQKK127 pKa = 9.77 RR128 pKa = 11.84 AVGSVEE134 pKa = 3.98 PKK136 pKa = 10.37 VLAFVQLLDD145 pKa = 3.54 QQIADD150 pKa = 3.41 MHH152 pKa = 6.61 AEE154 pKa = 3.97 RR155 pKa = 11.84 KK156 pKa = 9.6 KK157 pKa = 10.57 IQAEE161 pKa = 4.31 GQAKK165 pKa = 9.25 IDD167 pKa = 3.5 EE168 pKa = 4.57 LRR170 pKa = 11.84 RR171 pKa = 11.84 EE172 pKa = 3.99 IQRR175 pKa = 11.84 VEE177 pKa = 3.68 RR178 pKa = 11.84 EE179 pKa = 3.71 YY180 pKa = 11.37 DD181 pKa = 3.49 EE182 pKa = 4.47 KK183 pKa = 11.52 AKK185 pKa = 10.86 ASSKK189 pKa = 9.92 RR190 pKa = 11.84 FKK192 pKa = 10.15 PARR195 pKa = 11.84 KK196 pKa = 9.02 YY197 pKa = 10.82 VPPTKK202 pKa = 10.62 AKK204 pKa = 9.87 IDD206 pKa = 3.83 EE207 pKa = 4.6 EE208 pKa = 4.39 CWNAYY213 pKa = 8.88 LNKK216 pKa = 9.32 VQKK219 pKa = 10.16 SGKK222 pKa = 7.22 TAPEE226 pKa = 3.73 WNAVLQEE233 pKa = 4.23 QANTMYY239 pKa = 10.32 QQLYY243 pKa = 8.14 LTQHH247 pKa = 5.94 KK248 pKa = 10.46 QDD250 pKa = 3.44 FCGLEE255 pKa = 4.21 SNQIPLKK262 pKa = 10.33 VWADD266 pKa = 3.5 SKK268 pKa = 11.37 LKK270 pKa = 10.45 EE271 pKa = 4.19 LADD274 pKa = 3.27 NHH276 pKa = 5.11 EE277 pKa = 4.24 FRR279 pKa = 11.84 RR280 pKa = 11.84 VRR282 pKa = 11.84 EE283 pKa = 3.95 TQRR286 pKa = 11.84 ILGLASGVPSSPSASASKK304 pKa = 10.59 SLSGKK309 pKa = 8.42 QRR311 pKa = 11.84 SGGPSQSAGDD321 pKa = 3.94 EE322 pKa = 4.08 PVEE325 pKa = 4.05 EE326 pKa = 4.82 HH327 pKa = 6.76 GSSNSAKK334 pKa = 9.86 PAAPDD339 pKa = 3.38 MEE341 pKa = 4.2 ITDD344 pKa = 3.81 RR345 pKa = 11.84 DD346 pKa = 3.76 EE347 pKa = 4.72 EE348 pKa = 4.41 KK349 pKa = 10.35 TSSSKK354 pKa = 10.79 GKK356 pKa = 10.2 RR357 pKa = 11.84 KK358 pKa = 9.44 QKK360 pKa = 10.84 LKK362 pKa = 11.05 DD363 pKa = 3.04 RR364 pKa = 11.84 LAAKK368 pKa = 9.99 KK369 pKa = 10.78 SKK371 pKa = 10.46 DD372 pKa = 3.31 LSAKK376 pKa = 8.59 KK377 pKa = 8.27 TRR379 pKa = 11.84 KK380 pKa = 7.94 GTIRR384 pKa = 11.84 RR385 pKa = 11.84 DD386 pKa = 3.39 PHH388 pKa = 6.13 RR389 pKa = 11.84 KK390 pKa = 8.93 KK391 pKa = 10.85
Molecular weight: 43.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.784
IPC2_protein 8.756
IPC_protein 8.639
Toseland 9.765
ProMoST 9.326
Dawson 9.897
Bjellqvist 9.502
Wikipedia 9.984
Rodwell 10.511
Grimsley 9.926
Solomon 9.926
Lehninger 9.911
Nozaki 9.794
DTASelect 9.472
Thurlkill 9.78
EMBOSS 10.145
Sillero 9.823
Patrickios 10.16
IPC_peptide 9.926
IPC2_peptide 7.907
IPC2.peptide.svr19 7.692
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1448
391
1057
724.0
82.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.08 ± 1.134
1.312 ± 0.152
6.146 ± 0.139
6.492 ± 0.758
3.66 ± 0.851
5.11 ± 0.402
2.348 ± 0.159
5.387 ± 0.817
7.735 ± 1.72
9.599 ± 0.207
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.141 ± 0.32
3.729 ± 0.483
5.18 ± 0.304
4.696 ± 0.76
6.975 ± 0.037
6.285 ± 0.597
4.282 ± 0.1
6.492 ± 0.186
2.003 ± 0.382
2.348 ± 0.429
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here