Granulicatella sp. WM01

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Granulicatella; unclassified Granulicatella

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1694 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9J5Q9|A0A4Y9J5Q9_9LACT Uncharacterized protein OS=Granulicatella sp. WM01 OX=2558277 GN=E4T68_04965 PE=4 SV=1
MM1 pKa = 7.48SSALIVFASLTGSTEE16 pKa = 3.57EE17 pKa = 4.42CADD20 pKa = 4.01FVAEE24 pKa = 4.23KK25 pKa = 10.92LEE27 pKa = 3.99EE28 pKa = 4.13LGIAVDD34 pKa = 5.46IMDD37 pKa = 4.24STQASASDD45 pKa = 3.54FQNYY49 pKa = 8.91DD50 pKa = 3.08LCIVGSYY57 pKa = 9.88TYY59 pKa = 11.18GEE61 pKa = 4.43GEE63 pKa = 4.67IPDD66 pKa = 3.98EE67 pKa = 4.04MLDD70 pKa = 3.81FYY72 pKa = 11.59EE73 pKa = 6.14DD74 pKa = 3.61LAQEE78 pKa = 4.5DD79 pKa = 4.29LTGKK83 pKa = 10.01IFGVFGSGDD92 pKa = 3.33SYY94 pKa = 12.0YY95 pKa = 10.78EE96 pKa = 4.09FYY98 pKa = 11.23CNAVLLFEE106 pKa = 4.45EE107 pKa = 4.68QFKK110 pKa = 9.6KK111 pKa = 9.35TGATQGSEE119 pKa = 3.93NVKK122 pKa = 9.99IEE124 pKa = 5.12LMAEE128 pKa = 4.04DD129 pKa = 3.86QDD131 pKa = 4.38IEE133 pKa = 4.22NLHH136 pKa = 6.28KK137 pKa = 10.29FAEE140 pKa = 4.27EE141 pKa = 3.81LVQNLGKK148 pKa = 10.58

Molecular weight: 16.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9IWZ8|A0A4Y9IWZ8_9LACT HDIG domain-containing protein OS=Granulicatella sp. WM01 OX=2558277 GN=E4T68_07400 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 9.2KK9 pKa = 7.96RR10 pKa = 11.84HH11 pKa = 4.8RR12 pKa = 11.84QKK14 pKa = 10.16VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.73GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1694

0

1694

528419

26

2219

311.9

35.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.484 ± 0.076

0.826 ± 0.02

5.309 ± 0.048

6.83 ± 0.063

4.138 ± 0.051

5.989 ± 0.069

2.49 ± 0.035

8.037 ± 0.066

7.057 ± 0.061

9.75 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.533 ± 0.028

4.683 ± 0.051

3.075 ± 0.036

4.644 ± 0.05

3.844 ± 0.037

6.331 ± 0.115

6.035 ± 0.057

7.116 ± 0.056

0.798 ± 0.019

4.03 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski