Amino acid dipepetide frequency for Rheinheimera phage vB_RspM_Barba19A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.688AlaAla: 4.688 ± 0.543
0.985AlaCys: 0.985 ± 0.193
3.782AlaAsp: 3.782 ± 0.432
4.491AlaGlu: 4.491 ± 0.497
2.482AlaPhe: 2.482 ± 0.317
4.058AlaGly: 4.058 ± 0.462
0.788AlaHis: 0.788 ± 0.181
3.979AlaIle: 3.979 ± 0.355
4.767AlaLys: 4.767 ± 0.454
5.752AlaLeu: 5.752 ± 0.691
1.3AlaMet: 1.3 ± 0.231
2.915AlaAsn: 2.915 ± 0.277
1.773AlaPro: 1.773 ± 0.292
2.206AlaGln: 2.206 ± 0.314
2.049AlaArg: 2.049 ± 0.317
3.703AlaSer: 3.703 ± 0.431
4.018AlaThr: 4.018 ± 0.463
4.018AlaVal: 4.018 ± 0.487
0.591AlaTrp: 0.591 ± 0.147
3.309AlaTyr: 3.309 ± 0.392
0.0AlaXaa: 0.0 ± 0.0
Cys
0.512CysAla: 0.512 ± 0.149
0.276CysCys: 0.276 ± 0.104
1.064CysAsp: 1.064 ± 0.229
0.67CysGlu: 0.67 ± 0.173
0.749CysPhe: 0.749 ± 0.207
0.945CysGly: 0.945 ± 0.193
0.276CysHis: 0.276 ± 0.099
0.749CysIle: 0.749 ± 0.182
1.458CysLys: 1.458 ± 0.253
0.945CysLeu: 0.945 ± 0.188
0.512CysMet: 0.512 ± 0.132
0.827CysAsn: 0.827 ± 0.188
0.827CysPro: 0.827 ± 0.188
0.355CysGln: 0.355 ± 0.12
0.709CysArg: 0.709 ± 0.164
0.985CysSer: 0.985 ± 0.195
0.945CysThr: 0.945 ± 0.206
1.142CysVal: 1.142 ± 0.211
0.355CysTrp: 0.355 ± 0.106
0.552CysTyr: 0.552 ± 0.152
0.0CysXaa: 0.0 ± 0.0
Asp
3.427AspAla: 3.427 ± 0.35
0.945AspCys: 0.945 ± 0.139
3.782AspAsp: 3.782 ± 0.429
3.939AspGlu: 3.939 ± 0.445
2.639AspPhe: 2.639 ± 0.316
5.515AspGly: 5.515 ± 0.529
1.024AspHis: 1.024 ± 0.188
5.082AspIle: 5.082 ± 0.464
4.727AspLys: 4.727 ± 0.513
4.767AspLeu: 4.767 ± 0.531
1.261AspMet: 1.261 ± 0.216
3.23AspAsn: 3.23 ± 0.333
1.576AspPro: 1.576 ± 0.251
1.576AspGln: 1.576 ± 0.233
2.246AspArg: 2.246 ± 0.263
3.624AspSer: 3.624 ± 0.404
4.53AspThr: 4.53 ± 0.478
5.043AspVal: 5.043 ± 0.455
1.773AspTrp: 1.773 ± 0.295
3.27AspTyr: 3.27 ± 0.37
0.0AspXaa: 0.0 ± 0.0
Glu
3.743GluAla: 3.743 ± 0.431
1.142GluCys: 1.142 ± 0.256
4.058GluAsp: 4.058 ± 0.375
3.821GluGlu: 3.821 ± 0.42
2.758GluPhe: 2.758 ± 0.304
3.664GluGly: 3.664 ± 0.446
1.694GluHis: 1.694 ± 0.226
4.609GluIle: 4.609 ± 0.415
4.294GluLys: 4.294 ± 0.439
7.367GluLeu: 7.367 ± 0.627
1.97GluMet: 1.97 ± 0.3
3.546GluAsn: 3.546 ± 0.306
1.536GluPro: 1.536 ± 0.254
2.797GluGln: 2.797 ± 0.365
2.994GluArg: 2.994 ± 0.334
3.703GluSer: 3.703 ± 0.427
3.112GluThr: 3.112 ± 0.365
4.964GluVal: 4.964 ± 0.421
1.339GluTrp: 1.339 ± 0.244
3.743GluTyr: 3.743 ± 0.351
0.0GluXaa: 0.0 ± 0.0
Phe
2.679PheAla: 2.679 ± 0.362
0.749PheCys: 0.749 ± 0.168
2.994PheAsp: 2.994 ± 0.297
2.955PheGlu: 2.955 ± 0.332
1.852PhePhe: 1.852 ± 0.299
2.364PheGly: 2.364 ± 0.278
0.512PheHis: 0.512 ± 0.122
3.073PheIle: 3.073 ± 0.291
2.797PheLys: 2.797 ± 0.298
3.349PheLeu: 3.349 ± 0.294
1.3PheMet: 1.3 ± 0.196
3.23PheAsn: 3.23 ± 0.436
1.221PhePro: 1.221 ± 0.214
1.339PheGln: 1.339 ± 0.21
1.497PheArg: 1.497 ± 0.218
3.309PheSer: 3.309 ± 0.365
3.309PheThr: 3.309 ± 0.452
2.915PheVal: 2.915 ± 0.3
0.552PheTrp: 0.552 ± 0.147
1.261PheTyr: 1.261 ± 0.233
0.0PheXaa: 0.0 ± 0.0
Gly
3.664GlyAla: 3.664 ± 0.485
1.064GlyCys: 1.064 ± 0.185
4.294GlyAsp: 4.294 ± 0.588
3.703GlyGlu: 3.703 ± 0.338
3.27GlyPhe: 3.27 ± 0.38
4.609GlyGly: 4.609 ± 0.452
0.788GlyHis: 0.788 ± 0.174
3.191GlyIle: 3.191 ± 0.349
5.043GlyLys: 5.043 ± 0.509
5.318GlyLeu: 5.318 ± 0.468
1.891GlyMet: 1.891 ± 0.302
4.53GlyAsn: 4.53 ± 0.936
0.236GlyPro: 0.236 ± 0.104
1.93GlyGln: 1.93 ± 0.319
2.482GlyArg: 2.482 ± 0.351
5.633GlySer: 5.633 ± 0.516
3.979GlyThr: 3.979 ± 0.638
5.358GlyVal: 5.358 ± 0.468
1.497GlyTrp: 1.497 ± 0.298
3.388GlyTyr: 3.388 ± 0.685
0.0GlyXaa: 0.0 ± 0.0
His
0.749HisAla: 0.749 ± 0.183
0.158HisCys: 0.158 ± 0.09
0.67HisAsp: 0.67 ± 0.163
1.339HisGlu: 1.339 ± 0.216
0.512HisPhe: 0.512 ± 0.125
0.867HisGly: 0.867 ± 0.197
0.276HisHis: 0.276 ± 0.101
1.064HisIle: 1.064 ± 0.254
1.261HisLys: 1.261 ± 0.216
2.009HisLeu: 2.009 ± 0.285
0.276HisMet: 0.276 ± 0.097
0.906HisAsn: 0.906 ± 0.178
0.749HisPro: 0.749 ± 0.218
0.433HisGln: 0.433 ± 0.124
0.63HisArg: 0.63 ± 0.126
1.182HisSer: 1.182 ± 0.166
1.261HisThr: 1.261 ± 0.236
1.142HisVal: 1.142 ± 0.231
0.236HisTrp: 0.236 ± 0.089
0.788HisTyr: 0.788 ± 0.169
0.0HisXaa: 0.0 ± 0.0
Ile
3.624IleAla: 3.624 ± 0.292
0.67IleCys: 0.67 ± 0.197
4.53IleAsp: 4.53 ± 0.452
3.467IleGlu: 3.467 ± 0.502
1.97IlePhe: 1.97 ± 0.229
3.861IleGly: 3.861 ± 0.43
1.182IleHis: 1.182 ± 0.215
2.994IleIle: 2.994 ± 0.324
4.964IleLys: 4.964 ± 0.399
3.9IleLeu: 3.9 ± 0.397
1.3IleMet: 1.3 ± 0.217
3.467IleAsn: 3.467 ± 0.358
2.167IlePro: 2.167 ± 0.364
2.442IleGln: 2.442 ± 0.284
2.915IleArg: 2.915 ± 0.291
4.412IleSer: 4.412 ± 0.367
4.806IleThr: 4.806 ± 0.454
3.624IleVal: 3.624 ± 0.413
0.67IleTrp: 0.67 ± 0.145
2.758IleTyr: 2.758 ± 0.283
0.0IleXaa: 0.0 ± 0.0
Lys
4.018LysAla: 4.018 ± 0.488
0.945LysCys: 0.945 ± 0.209
4.058LysAsp: 4.058 ± 0.394
5.121LysGlu: 5.121 ± 0.534
2.994LysPhe: 2.994 ± 0.284
3.506LysGly: 3.506 ± 0.371
1.694LysHis: 1.694 ± 0.248
4.373LysIle: 4.373 ± 0.415
3.624LysLys: 3.624 ± 0.446
5.318LysLeu: 5.318 ± 0.495
2.324LysMet: 2.324 ± 0.337
3.033LysAsn: 3.033 ± 0.394
2.6LysPro: 2.6 ± 0.38
4.018LysGln: 4.018 ± 0.419
3.191LysArg: 3.191 ± 0.447
4.018LysSer: 4.018 ± 0.384
3.9LysThr: 3.9 ± 0.372
5.515LysVal: 5.515 ± 0.447
1.103LysTrp: 1.103 ± 0.225
3.309LysTyr: 3.309 ± 0.342
0.0LysXaa: 0.0 ± 0.0
Leu
5.436LeuAla: 5.436 ± 0.516
1.458LeuCys: 1.458 ± 0.21
4.964LeuAsp: 4.964 ± 0.441
6.5LeuGlu: 6.5 ± 0.46
3.861LeuPhe: 3.861 ± 0.536
4.846LeuGly: 4.846 ± 0.437
1.261LeuHis: 1.261 ± 0.224
4.649LeuIle: 4.649 ± 0.449
5.673LeuLys: 5.673 ± 0.506
6.894LeuLeu: 6.894 ± 0.586
2.167LeuMet: 2.167 ± 0.308
4.846LeuAsn: 4.846 ± 0.407
3.388LeuPro: 3.388 ± 0.383
3.585LeuGln: 3.585 ± 0.423
3.467LeuArg: 3.467 ± 0.349
6.382LeuSer: 6.382 ± 0.521
5.436LeuThr: 5.436 ± 0.508
5.555LeuVal: 5.555 ± 0.424
0.906LeuTrp: 0.906 ± 0.26
3.152LeuTyr: 3.152 ± 0.336
0.0LeuXaa: 0.0 ± 0.0
Met
1.812MetAla: 1.812 ± 0.242
0.433MetCys: 0.433 ± 0.124
0.945MetAsp: 0.945 ± 0.187
1.812MetGlu: 1.812 ± 0.342
1.3MetPhe: 1.3 ± 0.23
1.103MetGly: 1.103 ± 0.218
0.118MetHis: 0.118 ± 0.063
1.221MetIle: 1.221 ± 0.224
1.97MetLys: 1.97 ± 0.328
2.364MetLeu: 2.364 ± 0.348
0.749MetMet: 0.749 ± 0.187
1.221MetAsn: 1.221 ± 0.204
0.67MetPro: 0.67 ± 0.185
1.339MetGln: 1.339 ± 0.229
0.749MetArg: 0.749 ± 0.167
2.364MetSer: 2.364 ± 0.369
1.536MetThr: 1.536 ± 0.218
1.733MetVal: 1.733 ± 0.276
0.315MetTrp: 0.315 ± 0.114
0.945MetTyr: 0.945 ± 0.243
0.0MetXaa: 0.0 ± 0.0
Asn
3.152AsnAla: 3.152 ± 0.527
0.906AsnCys: 0.906 ± 0.301
2.876AsnAsp: 2.876 ± 0.366
2.876AsnGlu: 2.876 ± 0.384
2.049AsnPhe: 2.049 ± 0.288
4.53AsnGly: 4.53 ± 0.593
0.591AsnHis: 0.591 ± 0.182
3.191AsnIle: 3.191 ± 0.36
4.57AsnLys: 4.57 ± 0.456
4.176AsnLeu: 4.176 ± 0.389
1.418AsnMet: 1.418 ± 0.238
3.23AsnAsn: 3.23 ± 0.335
2.6AsnPro: 2.6 ± 0.336
2.482AsnGln: 2.482 ± 0.359
2.285AsnArg: 2.285 ± 0.33
2.679AsnSer: 2.679 ± 0.283
4.491AsnThr: 4.491 ± 1.184
4.058AsnVal: 4.058 ± 0.369
0.709AsnTrp: 0.709 ± 0.237
2.442AsnTyr: 2.442 ± 0.307
0.0AsnXaa: 0.0 ± 0.0
Pro
1.97ProAla: 1.97 ± 0.238
0.079ProCys: 0.079 ± 0.05
2.994ProAsp: 2.994 ± 0.363
2.758ProGlu: 2.758 ± 0.38
1.576ProPhe: 1.576 ± 0.26
0.039ProGly: 0.039 ± 0.035
0.473ProHis: 0.473 ± 0.124
2.364ProIle: 2.364 ± 0.298
1.773ProLys: 1.773 ± 0.286
2.679ProLeu: 2.679 ± 0.28
0.473ProMet: 0.473 ± 0.145
1.733ProAsn: 1.733 ± 0.303
0.906ProPro: 0.906 ± 0.204
1.064ProGln: 1.064 ± 0.225
1.103ProArg: 1.103 ± 0.243
2.009ProSer: 2.009 ± 0.288
3.191ProThr: 3.191 ± 0.403
3.191ProVal: 3.191 ± 0.439
0.315ProTrp: 0.315 ± 0.101
1.93ProTyr: 1.93 ± 0.246
0.0ProXaa: 0.0 ± 0.0
Gln
2.758GlnAla: 2.758 ± 0.44
0.512GlnCys: 0.512 ± 0.152
1.891GlnAsp: 1.891 ± 0.277
3.664GlnGlu: 3.664 ± 0.4
1.576GlnPhe: 1.576 ± 0.228
1.615GlnGly: 1.615 ± 0.259
0.788GlnHis: 0.788 ± 0.196
1.891GlnIle: 1.891 ± 0.268
2.285GlnLys: 2.285 ± 0.36
3.467GlnLeu: 3.467 ± 0.356
0.985GlnMet: 0.985 ± 0.277
2.246GlnAsn: 2.246 ± 0.504
1.536GlnPro: 1.536 ± 0.244
1.812GlnGln: 1.812 ± 0.36
2.049GlnArg: 2.049 ± 0.314
2.442GlnSer: 2.442 ± 0.31
1.97GlnThr: 1.97 ± 0.279
2.246GlnVal: 2.246 ± 0.299
0.394GlnTrp: 0.394 ± 0.13
1.97GlnTyr: 1.97 ± 0.295
0.0GlnXaa: 0.0 ± 0.0
Arg
2.482ArgAla: 2.482 ± 0.365
0.906ArgCys: 0.906 ± 0.213
2.285ArgAsp: 2.285 ± 0.352
2.246ArgGlu: 2.246 ± 0.279
1.891ArgPhe: 1.891 ± 0.274
2.561ArgGly: 2.561 ± 0.341
0.827ArgHis: 0.827 ± 0.17
2.442ArgIle: 2.442 ± 0.29
2.521ArgLys: 2.521 ± 0.325
3.743ArgLeu: 3.743 ± 0.402
1.024ArgMet: 1.024 ± 0.219
2.364ArgAsn: 2.364 ± 0.468
1.458ArgPro: 1.458 ± 0.231
1.733ArgGln: 1.733 ± 0.286
1.694ArgArg: 1.694 ± 0.271
2.206ArgSer: 2.206 ± 0.28
2.285ArgThr: 2.285 ± 0.269
3.033ArgVal: 3.033 ± 0.389
0.433ArgTrp: 0.433 ± 0.11
2.009ArgTyr: 2.009 ± 0.25
0.0ArgXaa: 0.0 ± 0.0
Ser
4.097SerAla: 4.097 ± 0.554
1.103SerCys: 1.103 ± 0.199
5.2SerAsp: 5.2 ± 0.462
3.9SerGlu: 3.9 ± 0.402
3.152SerPhe: 3.152 ± 0.328
4.964SerGly: 4.964 ± 0.462
1.064SerHis: 1.064 ± 0.204
3.427SerIle: 3.427 ± 0.366
4.806SerLys: 4.806 ± 0.397
5.476SerLeu: 5.476 ± 0.569
1.615SerMet: 1.615 ± 0.257
3.546SerAsn: 3.546 ± 0.394
2.364SerPro: 2.364 ± 0.339
2.167SerGln: 2.167 ± 0.238
2.442SerArg: 2.442 ± 0.296
4.806SerSer: 4.806 ± 0.568
3.743SerThr: 3.743 ± 0.473
4.57SerVal: 4.57 ± 0.522
0.788SerTrp: 0.788 ± 0.173
2.955SerTyr: 2.955 ± 0.39
0.0SerXaa: 0.0 ± 0.0
Thr
4.924ThrAla: 4.924 ± 0.692
0.512ThrCys: 0.512 ± 0.169
4.333ThrAsp: 4.333 ± 0.372
3.939ThrGlu: 3.939 ± 0.345
2.758ThrPhe: 2.758 ± 0.344
6.54ThrGly: 6.54 ± 1.252
1.024ThrHis: 1.024 ± 0.211
4.176ThrIle: 4.176 ± 0.435
4.136ThrLys: 4.136 ± 0.398
4.846ThrLeu: 4.846 ± 0.525
0.945ThrMet: 0.945 ± 0.181
3.309ThrAsn: 3.309 ± 0.369
2.639ThrPro: 2.639 ± 0.383
2.285ThrGln: 2.285 ± 0.318
1.812ThrArg: 1.812 ± 0.239
3.782ThrSer: 3.782 ± 0.418
4.609ThrThr: 4.609 ± 0.502
4.806ThrVal: 4.806 ± 0.381
0.63ThrTrp: 0.63 ± 0.145
2.324ThrTyr: 2.324 ± 0.298
0.0ThrXaa: 0.0 ± 0.0
Val
4.609ValAla: 4.609 ± 0.556
0.906ValCys: 0.906 ± 0.19
5.279ValAsp: 5.279 ± 0.478
5.633ValGlu: 5.633 ± 0.499
3.309ValPhe: 3.309 ± 0.349
5.476ValGly: 5.476 ± 0.465
1.221ValHis: 1.221 ± 0.256
3.664ValIle: 3.664 ± 0.352
4.215ValLys: 4.215 ± 0.4
4.924ValLeu: 4.924 ± 0.426
1.93ValMet: 1.93 ± 0.28
3.821ValAsn: 3.821 ± 0.363
2.6ValPro: 2.6 ± 0.348
2.206ValGln: 2.206 ± 0.286
3.033ValArg: 3.033 ± 0.309
5.121ValSer: 5.121 ± 0.504
4.176ValThr: 4.176 ± 0.386
6.303ValVal: 6.303 ± 0.54
1.418ValTrp: 1.418 ± 0.236
2.797ValTyr: 2.797 ± 0.293
0.0ValXaa: 0.0 ± 0.0
Trp
0.67TrpAla: 0.67 ± 0.148
0.158TrpCys: 0.158 ± 0.083
0.552TrpAsp: 0.552 ± 0.151
1.024TrpGlu: 1.024 ± 0.19
0.749TrpPhe: 0.749 ± 0.17
0.709TrpGly: 0.709 ± 0.151
0.197TrpHis: 0.197 ± 0.086
0.827TrpIle: 0.827 ± 0.167
0.985TrpLys: 0.985 ± 0.226
1.852TrpLeu: 1.852 ± 0.317
0.355TrpMet: 0.355 ± 0.119
0.749TrpAsn: 0.749 ± 0.362
0.394TrpPro: 0.394 ± 0.136
0.945TrpGln: 0.945 ± 0.163
0.788TrpArg: 0.788 ± 0.202
0.827TrpSer: 0.827 ± 0.187
0.788TrpThr: 0.788 ± 0.168
1.182TrpVal: 1.182 ± 0.205
0.355TrpTrp: 0.355 ± 0.131
0.473TrpTyr: 0.473 ± 0.145
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.639TyrAla: 2.639 ± 0.26
0.945TyrCys: 0.945 ± 0.219
3.427TyrAsp: 3.427 ± 0.46
2.994TyrGlu: 2.994 ± 0.274
1.812TyrPhe: 1.812 ± 0.24
4.097TyrGly: 4.097 ± 0.673
0.709TyrHis: 0.709 ± 0.17
2.758TyrIle: 2.758 ± 0.321
2.797TyrLys: 2.797 ± 0.342
5.279TyrLeu: 5.279 ± 0.542
0.867TyrMet: 0.867 ± 0.189
2.561TyrAsn: 2.561 ± 0.41
1.379TyrPro: 1.379 ± 0.262
1.379TyrGln: 1.379 ± 0.238
2.009TyrArg: 2.009 ± 0.291
2.994TyrSer: 2.994 ± 0.309
2.246TyrThr: 2.246 ± 0.325
2.285TyrVal: 2.285 ± 0.333
0.197TyrTrp: 0.197 ± 0.077
2.246TyrTyr: 2.246 ± 0.264
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 143 proteins (25385 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski