Amino acid dipepetide frequency for Bombyx mori (Silk moth)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.535AlaAla: 7.535 ± 0.081	1.426AlaCys: 1.426 ± 0.039	3.549AlaAsp: 3.549 ± 0.028	4.373AlaGlu: 4.373 ± 0.039	2.316AlaPhe: 2.316 ± 0.025	4.596AlaGly: 4.596 ± 0.269	1.824AlaHis: 1.824 ± 0.024	3.397AlaIle: 3.397 ± 0.029	3.635AlaLys: 3.635 ± 0.029	7.152AlaLeu: 7.152 ± 0.065	1.514AlaMet: 1.514 ± 0.017	2.626AlaAsn: 2.626 ± 0.024	4.173AlaPro: 4.173 ± 0.046	2.598AlaGln: 2.598 ± 0.026	4.224AlaArg: 4.224 ± 0.044	5.011AlaSer: 5.011 ± 0.039	3.867AlaThr: 3.867 ± 0.032	4.868AlaVal: 4.868 ± 0.038	0.761AlaTrp: 0.761 ± 0.012	1.909AlaTyr: 1.909 ± 0.026	0.002AlaXaa: 0.002 ± 0.001
Cys	1.416CysAla: 1.416 ± 0.024	0.476CysCys: 0.476 ± 0.012	1.191CysAsp: 1.191 ± 0.022	1.246CysGlu: 1.246 ± 0.025	0.698CysPhe: 0.698 ± 0.014	1.545CysGly: 1.545 ± 0.046	0.487CysHis: 0.487 ± 0.012	1.005CysIle: 1.005 ± 0.039	1.092CysLys: 1.092 ± 0.03	1.752CysLeu: 1.752 ± 0.033	0.379CysMet: 0.379 ± 0.009	0.853CysAsn: 0.853 ± 0.02	1.061CysPro: 1.061 ± 0.038	0.673CysGln: 0.673 ± 0.023	1.179CysArg: 1.179 ± 0.037	1.534CysSer: 1.534 ± 0.041	1.109CysThr: 1.109 ± 0.028	1.391CysVal: 1.391 ± 0.034	0.226CysTrp: 0.226 ± 0.006	0.596CysTyr: 0.596 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	3.63AspAla: 3.63 ± 0.031	1.049AspCys: 1.049 ± 0.024	3.964AspAsp: 3.964 ± 0.039	4.404AspGlu: 4.404 ± 0.035	2.149AspPhe: 2.149 ± 0.022	3.277AspGly: 3.277 ± 0.031	1.199AspHis: 1.199 ± 0.015	3.345AspIle: 3.345 ± 0.027	3.482AspLys: 3.482 ± 0.035	4.94AspLeu: 4.94 ± 0.035	1.243AspMet: 1.243 ± 0.015	2.72AspAsn: 2.72 ± 0.026	2.673AspPro: 2.673 ± 0.04	1.644AspGln: 1.644 ± 0.018	2.787AspArg: 2.787 ± 0.034	4.289AspSer: 4.289 ± 0.037	2.972AspThr: 2.972 ± 0.025	3.918AspVal: 3.918 ± 0.029	0.653AspTrp: 0.653 ± 0.012	1.929AspTyr: 1.929 ± 0.018	0.002AspXaa: 0.002 ± 0.0
Glu	4.431GluAla: 4.431 ± 0.038	1.297GluCys: 1.297 ± 0.055	4.078GluAsp: 4.078 ± 0.036	5.733GluGlu: 5.733 ± 0.061	2.131GluPhe: 2.131 ± 0.024	3.181GluGly: 3.181 ± 0.028	1.566GluHis: 1.566 ± 0.022	3.972GluIle: 3.972 ± 0.038	5.025GluLys: 5.025 ± 0.057	6.036GluLeu: 6.036 ± 0.049	1.58GluMet: 1.58 ± 0.018	3.691GluAsn: 3.691 ± 0.037	2.997GluPro: 2.997 ± 0.035	2.605GluGln: 2.605 ± 0.029	3.991GluArg: 3.991 ± 0.038	4.36GluSer: 4.36 ± 0.037	3.83GluThr: 3.83 ± 0.038	3.904GluVal: 3.904 ± 0.03	0.715GluTrp: 0.715 ± 0.013	2.076GluTyr: 2.076 ± 0.022	0.001GluXaa: 0.001 ± 0.0
Phe	2.174PheAla: 2.174 ± 0.026	0.695PheCys: 0.695 ± 0.01	2.077PheAsp: 2.077 ± 0.021	2.19PheGlu: 2.19 ± 0.021	1.365PhePhe: 1.365 ± 0.02	2.33PheGly: 2.33 ± 0.032	0.872PheHis: 0.872 ± 0.012	2.005PheIle: 2.005 ± 0.021	2.156PheLys: 2.156 ± 0.022	3.164PheLeu: 3.164 ± 0.029	0.851PheMet: 0.851 ± 0.014	1.773PheAsn: 1.773 ± 0.02	1.579PhePro: 1.579 ± 0.017	1.259PheGln: 1.259 ± 0.013	1.847PheArg: 1.847 ± 0.017	2.694PheSer: 2.694 ± 0.026	2.047PheThr: 2.047 ± 0.023	2.434PheVal: 2.434 ± 0.024	0.407PheTrp: 0.407 ± 0.009	1.308PheTyr: 1.308 ± 0.015	0.001PheXaa: 0.001 ± 0.0
Gly	4.63GlyAla: 4.63 ± 0.279	1.084GlyCys: 1.084 ± 0.022	3.19GlyAsp: 3.19 ± 0.035	3.279GlyGlu: 3.279 ± 0.031	2.173GlyPhe: 2.173 ± 0.024	4.666GlyGly: 4.666 ± 0.065	1.507GlyHis: 1.507 ± 0.023	2.864GlyIle: 2.864 ± 0.025	3.211GlyLys: 3.211 ± 0.031	4.689GlyLeu: 4.689 ± 0.042	1.152GlyMet: 1.152 ± 0.017	2.455GlyAsn: 2.455 ± 0.026	2.654GlyPro: 2.654 ± 0.046	2.014GlyGln: 2.014 ± 0.044	3.269GlyArg: 3.269 ± 0.029	4.475GlySer: 4.475 ± 0.102	3.182GlyThr: 3.182 ± 0.028	3.791GlyVal: 3.791 ± 0.035	0.743GlyTrp: 0.743 ± 0.013	2.093GlyTyr: 2.093 ± 0.055	0.002GlyXaa: 0.002 ± 0.0
His	1.747HisAla: 1.747 ± 0.023	0.58HisCys: 0.58 ± 0.012	1.251HisAsp: 1.251 ± 0.016	1.448HisGlu: 1.448 ± 0.018	0.965HisPhe: 0.965 ± 0.014	1.443HisGly: 1.443 ± 0.023	0.962HisHis: 0.962 ± 0.023	1.326HisIle: 1.326 ± 0.016	1.419HisLys: 1.419 ± 0.018	2.348HisLeu: 2.348 ± 0.025	0.634HisMet: 0.634 ± 0.014	1.164HisAsn: 1.164 ± 0.015	1.344HisPro: 1.344 ± 0.019	0.926HisGln: 0.926 ± 0.014	1.519HisArg: 1.519 ± 0.02	1.907HisSer: 1.907 ± 0.023	1.396HisThr: 1.396 ± 0.016	1.655HisVal: 1.655 ± 0.018	0.327HisTrp: 0.327 ± 0.008	0.935HisTyr: 0.935 ± 0.013	0.001HisXaa: 0.001 ± 0.0
Ile	3.464IleAla: 3.464 ± 0.029	1.126IleCys: 1.126 ± 0.029	3.164IleAsp: 3.164 ± 0.029	3.726IleGlu: 3.726 ± 0.039	1.991IlePhe: 1.991 ± 0.025	2.736IleGly: 2.736 ± 0.026	1.259IleHis: 1.259 ± 0.015	3.096IleIle: 3.096 ± 0.033	3.653IleLys: 3.653 ± 0.033	4.719IleLeu: 4.719 ± 0.035	1.18IleMet: 1.18 ± 0.013	2.757IleAsn: 2.757 ± 0.031	2.762IlePro: 2.762 ± 0.024	2.091IleGln: 2.091 ± 0.021	2.63IleArg: 2.63 ± 0.023	3.981IleSer: 3.981 ± 0.024	3.164IleThr: 3.164 ± 0.028	3.549IleVal: 3.549 ± 0.029	0.527IleTrp: 0.527 ± 0.011	1.706IleTyr: 1.706 ± 0.023	0.002IleXaa: 0.002 ± 0.001
Lys	3.589LysAla: 3.589 ± 0.034	1.197LysCys: 1.197 ± 0.031	3.491LysAsp: 3.491 ± 0.032	4.68LysGlu: 4.68 ± 0.047	2.105LysPhe: 2.105 ± 0.022	2.733LysGly: 2.733 ± 0.035	1.594LysHis: 1.594 ± 0.019	3.79LysIle: 3.79 ± 0.03	5.348LysLys: 5.348 ± 0.055	5.709LysLeu: 5.709 ± 0.041	1.534LysMet: 1.534 ± 0.02	3.28LysAsn: 3.28 ± 0.032	3.212LysPro: 3.212 ± 0.039	2.549LysGln: 2.549 ± 0.024	3.727LysArg: 3.727 ± 0.029	4.525LysSer: 4.525 ± 0.039	3.71LysThr: 3.71 ± 0.033	3.602LysVal: 3.602 ± 0.029	0.645LysTrp: 0.645 ± 0.012	2.251LysTyr: 2.251 ± 0.024	0.002LysXaa: 0.002 ± 0.001
Leu	6.708LeuAla: 6.708 ± 0.048	1.877LeuCys: 1.877 ± 0.025	4.943LeuAsp: 4.943 ± 0.04	5.953LeuGlu: 5.953 ± 0.054	3.062LeuPhe: 3.062 ± 0.03	4.682LeuGly: 4.682 ± 0.036	2.44LeuHis: 2.44 ± 0.026	4.325LeuIle: 4.325 ± 0.037	6.068LeuLys: 6.068 ± 0.049	8.637LeuLeu: 8.637 ± 0.064	1.979LeuMet: 1.979 ± 0.023	4.221LeuAsn: 4.221 ± 0.031	4.762LeuPro: 4.762 ± 0.037	4.2LeuGln: 4.2 ± 0.046	5.584LeuArg: 5.584 ± 0.041	6.786LeuSer: 6.786 ± 0.048	5.05LeuThr: 5.05 ± 0.034	5.514LeuVal: 5.514 ± 0.045	0.964LeuTrp: 0.964 ± 0.016	2.755LeuTyr: 2.755 ± 0.026	0.003LeuXaa: 0.003 ± 0.001
Met	1.657MetAla: 1.657 ± 0.02	0.448MetCys: 0.448 ± 0.012	1.193MetAsp: 1.193 ± 0.015	1.493MetGlu: 1.493 ± 0.017	0.907MetPhe: 0.907 ± 0.014	1.123MetGly: 1.123 ± 0.019	0.489MetHis: 0.489 ± 0.009	1.074MetIle: 1.074 ± 0.015	1.53MetLys: 1.53 ± 0.02	2.0MetLeu: 2.0 ± 0.019	0.612MetMet: 0.612 ± 0.012	1.045MetAsn: 1.045 ± 0.015	1.127MetPro: 1.127 ± 0.017	0.884MetGln: 0.884 ± 0.015	1.217MetArg: 1.217 ± 0.018	1.813MetSer: 1.813 ± 0.021	1.225MetThr: 1.225 ± 0.016	1.287MetVal: 1.287 ± 0.017	0.26MetTrp: 0.26 ± 0.007	0.713MetTyr: 0.713 ± 0.013	0.001MetXaa: 0.001 ± 0.0
Asn	2.815AsnAla: 2.815 ± 0.031	0.851AsnCys: 0.851 ± 0.017	2.651AsnAsp: 2.651 ± 0.024	3.274AsnGlu: 3.274 ± 0.03	1.8AsnPhe: 1.8 ± 0.019	2.764AsnGly: 2.764 ± 0.032	1.03AsnHis: 1.03 ± 0.02	3.163AsnIle: 3.163 ± 0.03	3.283AsnLys: 3.283 ± 0.031	4.178AsnLeu: 4.178 ± 0.037	1.169AsnMet: 1.169 ± 0.015	2.877AsnAsn: 2.877 ± 0.037	2.207AsnPro: 2.207 ± 0.038	1.729AsnGln: 1.729 ± 0.028	2.239AsnArg: 2.239 ± 0.025	3.535AsnSer: 3.535 ± 0.032	2.747AsnThr: 2.747 ± 0.026	3.291AsnVal: 3.291 ± 0.026	0.481AsnTrp: 0.481 ± 0.01	1.677AsnTyr: 1.677 ± 0.019	0.002AsnXaa: 0.002 ± 0.001
Pro	4.198ProAla: 4.198 ± 0.047	0.865ProCys: 0.865 ± 0.058	2.91ProAsp: 2.91 ± 0.026	3.675ProGlu: 3.675 ± 0.034	1.676ProPhe: 1.676 ± 0.02	3.04ProGly: 3.04 ± 0.07	1.429ProHis: 1.429 ± 0.022	2.538ProIle: 2.538 ± 0.027	2.993ProLys: 2.993 ± 0.03	4.355ProLeu: 4.355 ± 0.034	0.964ProMet: 0.964 ± 0.016	2.262ProAsn: 2.262 ± 0.031	4.898ProPro: 4.898 ± 0.073	2.173ProGln: 2.173 ± 0.031	2.985ProArg: 2.985 ± 0.035	4.115ProSer: 4.115 ± 0.046	3.145ProThr: 3.145 ± 0.037	3.563ProVal: 3.563 ± 0.036	0.507ProTrp: 0.507 ± 0.01	1.675ProTyr: 1.675 ± 0.025	0.003ProXaa: 0.003 ± 0.001
Gln	2.536GlnAla: 2.536 ± 0.026	0.81GlnCys: 0.81 ± 0.023	1.82GlnAsp: 1.82 ± 0.02	2.531GlnGlu: 2.531 ± 0.028	1.316GlnPhe: 1.316 ± 0.015	1.765GlnGly: 1.765 ± 0.025	1.156GlnHis: 1.156 ± 0.018	2.132GlnIle: 2.132 ± 0.022	2.38GlnLys: 2.38 ± 0.025	3.751GlnLeu: 3.751 ± 0.037	0.95GlnMet: 0.95 ± 0.015	2.103GlnAsn: 2.103 ± 0.025	2.125GlnPro: 2.125 ± 0.041	2.186GlnGln: 2.186 ± 0.04	2.409GlnArg: 2.409 ± 0.028	2.61GlnSer: 2.61 ± 0.026	2.105GlnThr: 2.105 ± 0.023	2.167GlnVal: 2.167 ± 0.023	0.458GlnTrp: 0.458 ± 0.009	1.363GlnTyr: 1.363 ± 0.018	0.001GlnXaa: 0.001 ± 0.0
Arg	4.339ArgAla: 4.339 ± 0.049	1.142ArgCys: 1.142 ± 0.022	3.207ArgAsp: 3.207 ± 0.03	3.614ArgGlu: 3.614 ± 0.035	1.831ArgPhe: 1.831 ± 0.019	3.223ArgGly: 3.223 ± 0.035	1.66ArgHis: 1.66 ± 0.02	2.796ArgIle: 2.796 ± 0.025	3.582ArgLys: 3.582 ± 0.031	5.307ArgLeu: 5.307 ± 0.045	1.203ArgMet: 1.203 ± 0.016	2.596ArgAsn: 2.596 ± 0.023	3.039ArgPro: 3.039 ± 0.035	2.223ArgGln: 2.223 ± 0.024	4.606ArgArg: 4.606 ± 0.049	4.075ArgSer: 4.075 ± 0.041	3.021ArgThr: 3.021 ± 0.029	3.404ArgVal: 3.404 ± 0.03	0.661ArgTrp: 0.661 ± 0.012	1.78ArgTyr: 1.78 ± 0.022	0.002ArgXaa: 0.002 ± 0.001
Ser	5.075SerAla: 5.075 ± 0.038	1.411SerCys: 1.411 ± 0.045	4.415SerAsp: 4.415 ± 0.039	4.853SerGlu: 4.853 ± 0.037	2.542SerPhe: 2.542 ± 0.023	4.717SerGly: 4.717 ± 0.105	1.725SerHis: 1.725 ± 0.019	3.702SerIle: 3.702 ± 0.031	4.427SerLys: 4.427 ± 0.04	6.579SerLeu: 6.579 ± 0.045	1.525SerMet: 1.525 ± 0.019	3.538SerAsn: 3.538 ± 0.036	4.373SerPro: 4.373 ± 0.053	2.776SerGln: 2.776 ± 0.029	4.05SerArg: 4.05 ± 0.036	7.069SerSer: 7.069 ± 0.069	4.681SerThr: 4.681 ± 0.054	4.781SerVal: 4.781 ± 0.031	0.837SerTrp: 0.837 ± 0.014	2.196SerTyr: 2.196 ± 0.023	0.003SerXaa: 0.003 ± 0.001
Thr	4.062ThrAla: 4.062 ± 0.03	1.131ThrCys: 1.131 ± 0.026	3.129ThrAsp: 3.129 ± 0.029	3.865ThrGlu: 3.865 ± 0.049	2.062ThrPhe: 2.062 ± 0.021	3.26ThrGly: 3.26 ± 0.033	1.383ThrHis: 1.383 ± 0.017	3.074ThrIle: 3.074 ± 0.026	3.475ThrLys: 3.475 ± 0.032	5.223ThrLeu: 5.223 ± 0.037	1.156ThrMet: 1.156 ± 0.016	2.68ThrAsn: 2.68 ± 0.024	3.541ThrPro: 3.541 ± 0.041	2.047ThrGln: 2.047 ± 0.023	2.824ThrArg: 2.824 ± 0.025	4.519ThrSer: 4.519 ± 0.042	3.906ThrThr: 3.906 ± 0.099	4.013ThrVal: 4.013 ± 0.033	0.619ThrTrp: 0.619 ± 0.011	1.683ThrTyr: 1.683 ± 0.022	0.002ThrXaa: 0.002 ± 0.0
Val	4.708ValAla: 4.708 ± 0.039	1.457ValCys: 1.457 ± 0.029	3.522ValAsp: 3.522 ± 0.03	4.135ValGlu: 4.135 ± 0.036	2.378ValPhe: 2.378 ± 0.024	3.305ValGly: 3.305 ± 0.031	1.6ValHis: 1.6 ± 0.02	3.39ValIle: 3.39 ± 0.029	3.845ValLys: 3.845 ± 0.028	5.986ValLeu: 5.986 ± 0.045	1.423ValMet: 1.423 ± 0.017	2.918ValAsn: 2.918 ± 0.024	3.493ValPro: 3.493 ± 0.033	2.45ValGln: 2.45 ± 0.024	3.606ValArg: 3.606 ± 0.034	4.762ValSer: 4.762 ± 0.033	4.036ValThr: 4.036 ± 0.033	4.505ValVal: 4.505 ± 0.038	0.764ValTrp: 0.764 ± 0.014	2.022ValTyr: 2.022 ± 0.02	0.002ValXaa: 0.002 ± 0.001
Trp	0.686TrpAla: 0.686 ± 0.013	0.248TrpCys: 0.248 ± 0.007	0.636TrpAsp: 0.636 ± 0.012	0.648TrpGlu: 0.648 ± 0.01	0.435TrpPhe: 0.435 ± 0.01	0.661TrpGly: 0.661 ± 0.013	0.265TrpHis: 0.265 ± 0.008	0.552TrpIle: 0.552 ± 0.01	0.687TrpLys: 0.687 ± 0.012	1.122TrpLeu: 1.122 ± 0.018	0.291TrpMet: 0.291 ± 0.007	0.535TrpAsn: 0.535 ± 0.011	0.481TrpPro: 0.481 ± 0.011	0.434TrpGln: 0.434 ± 0.01	0.787TrpArg: 0.787 ± 0.014	0.85TrpSer: 0.85 ± 0.015	0.618TrpThr: 0.618 ± 0.012	0.639TrpVal: 0.639 ± 0.011	0.203TrpTrp: 0.203 ± 0.007	0.373TrpTyr: 0.373 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.956TyrAla: 1.956 ± 0.02	0.737TyrCys: 0.737 ± 0.013	1.884TyrAsp: 1.884 ± 0.019	2.08TyrGlu: 2.08 ± 0.023	1.323TyrPhe: 1.323 ± 0.017	2.075TyrGly: 2.075 ± 0.047	0.847TyrHis: 0.847 ± 0.011	1.735TyrIle: 1.735 ± 0.021	1.979TyrLys: 1.979 ± 0.023	2.864TyrLeu: 2.864 ± 0.029	0.756TyrMet: 0.756 ± 0.012	1.666TyrAsn: 1.666 ± 0.02	1.468TyrPro: 1.468 ± 0.023	1.211TyrGln: 1.211 ± 0.015	1.828TyrArg: 1.828 ± 0.021	2.366TyrSer: 2.366 ± 0.024	1.841TyrThr: 1.841 ± 0.02	2.031TyrVal: 2.031 ± 0.019	0.392TyrTrp: 0.392 ± 0.012	1.281TyrTyr: 1.281 ± 0.019	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.001XaaGlu: 0.001 ± 0.0	0.002XaaPhe: 0.002 ± 0.001	0.002XaaGly: 0.002 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.002XaaLys: 0.002 ± 0.0	0.003XaaLeu: 0.003 ± 0.001	0.002XaaMet: 0.002 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.001XaaGln: 0.001 ± 0.0	0.003XaaArg: 0.003 ± 0.001	0.003XaaSer: 0.003 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.001XaaVal: 0.001 ± 0.0	0.001XaaTrp: 0.001 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.001XaaXaa: 0.001 ± 0.0

Statistics based on 14787 proteins (5961797 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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