Duck hepatitis B virus (strain Germany/DHBV-3) (DHBV)
Average proteome isoelectric point is 8.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P0C691|DPOL_DHBV3 Protein P OS=Duck hepatitis B virus (strain Germany/DHBV-3) OX=489542 GN=P PE=1 SV=1
MM1 pKa = 7.08 GQHH4 pKa = 6.83 PAKK7 pKa = 10.86 SMDD10 pKa = 2.88 VRR12 pKa = 11.84 RR13 pKa = 11.84 IEE15 pKa = 4.69 GGEE18 pKa = 3.94 LLLNQLAGRR27 pKa = 11.84 MIPKK31 pKa = 8.61 GTLTWSGKK39 pKa = 10.12 FPTIDD44 pKa = 2.93 HH45 pKa = 6.27 VLDD48 pKa = 3.79 HH49 pKa = 6.1 VQTMEE54 pKa = 5.18 EE55 pKa = 4.51 INTLQQQGAWPAGAGRR71 pKa = 11.84 RR72 pKa = 11.84 VGLSNPAPQEE82 pKa = 3.66 IPQPQWTPEE91 pKa = 3.83 EE92 pKa = 4.01 DD93 pKa = 3.23 QKK95 pKa = 11.68 ARR97 pKa = 11.84 EE98 pKa = 3.95 AFRR101 pKa = 11.84 RR102 pKa = 11.84 YY103 pKa = 8.96 QEE105 pKa = 3.91 EE106 pKa = 4.36 RR107 pKa = 11.84 PPEE110 pKa = 4.18 TTTIPPTSPTQWKK123 pKa = 8.42 LQPGDD128 pKa = 4.58 DD129 pKa = 4.21 PLLGNQSLLEE139 pKa = 4.06 THH141 pKa = 7.31 PLYY144 pKa = 9.63 QTEE147 pKa = 4.09 PAVPVIKK154 pKa = 9.85 TPPLKK159 pKa = 10.58 KK160 pKa = 10.34 KK161 pKa = 10.19 MSGTFGGILAGLIGLLVSFFLLIKK185 pKa = 10.07 ILEE188 pKa = 4.06 ILRR191 pKa = 11.84 RR192 pKa = 11.84 LDD194 pKa = 3.1 WWWISLSSPKK204 pKa = 10.95 GKK206 pKa = 8.31 MQCAFQDD213 pKa = 3.1 TGAQISPHH221 pKa = 5.65 YY222 pKa = 10.44 AGSCPWGCPGFLWTYY237 pKa = 10.39 LRR239 pKa = 11.84 LFIIFLLILLVAAGLLYY256 pKa = 10.14 LTDD259 pKa = 3.77 NGSTILGKK267 pKa = 9.65 LQWASVSALFSSISSLLPSDD287 pKa = 3.98 PKK289 pKa = 11.3 SLVALTFGLSLIWMTSSSATQTLVTLTQLATLSALFYY326 pKa = 10.91 KK327 pKa = 10.61 SS328 pKa = 2.99
Molecular weight: 36.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.617
IPC2_protein 7.849
IPC_protein 7.878
Toseland 7.966
ProMoST 8.273
Dawson 8.58
Bjellqvist 8.712
Wikipedia 8.551
Rodwell 8.609
Grimsley 7.79
Solomon 8.799
Lehninger 8.799
Nozaki 8.902
DTASelect 8.536
Thurlkill 8.624
EMBOSS 8.799
Sillero 8.887
Patrickios 4.698
IPC_peptide 8.799
IPC2_peptide 7.541
IPC2.peptide.svr19 7.61
Protein with the highest isoelectric point:
>sp|P0C6J9|HBEAG_DHBV3 External core antigen OS=Duck hepatitis B virus (strain Germany/DHBV-3) OX=489542 GN=C PE=3 SV=1
MM1 pKa = 8.23 DD2 pKa = 4.61 INASRR7 pKa = 11.84 ALANVYY13 pKa = 10.06 DD14 pKa = 4.55 LPDD17 pKa = 4.02 DD18 pKa = 4.66 FFPKK22 pKa = 9.79 IDD24 pKa = 4.28 DD25 pKa = 4.0 LVRR28 pKa = 11.84 DD29 pKa = 4.15 AKK31 pKa = 10.95 DD32 pKa = 2.99 ALEE35 pKa = 4.58 PYY37 pKa = 8.58 WRR39 pKa = 11.84 SDD41 pKa = 3.62 SIKK44 pKa = 10.32 KK45 pKa = 9.66 HH46 pKa = 4.88 VLIATHH52 pKa = 6.68 FVDD55 pKa = 6.26 LIEE58 pKa = 5.8 DD59 pKa = 4.56 FWQTTQGMHH68 pKa = 7.15 EE69 pKa = 4.02 IAEE72 pKa = 4.32 ALRR75 pKa = 11.84 AVIPPTTTPVPQGYY89 pKa = 9.89 LIQHH93 pKa = 6.94 DD94 pKa = 4.2 EE95 pKa = 4.3 AEE97 pKa = 4.83 EE98 pKa = 4.16 IPLGDD103 pKa = 3.73 LFKK106 pKa = 10.75 HH107 pKa = 4.38 QEE109 pKa = 3.68 EE110 pKa = 5.1 RR111 pKa = 11.84 IVSFQPDD118 pKa = 3.7 YY119 pKa = 10.99 PITARR124 pKa = 11.84 IHH126 pKa = 5.86 AHH128 pKa = 5.52 LKK130 pKa = 10.23 AYY132 pKa = 10.51 AKK134 pKa = 10.36 INEE137 pKa = 4.18 EE138 pKa = 3.87 SLDD141 pKa = 3.51 RR142 pKa = 11.84 ARR144 pKa = 11.84 RR145 pKa = 11.84 LLWWHH150 pKa = 5.78 YY151 pKa = 11.24 NCLLWGEE158 pKa = 4.52 ANVTNYY164 pKa = 9.84 ISRR167 pKa = 11.84 LRR169 pKa = 11.84 TWLSTPEE176 pKa = 3.92 RR177 pKa = 11.84 YY178 pKa = 9.54 RR179 pKa = 11.84 GRR181 pKa = 11.84 DD182 pKa = 3.19 APTIEE187 pKa = 5.55 AITRR191 pKa = 11.84 PIQVAQGGRR200 pKa = 11.84 KK201 pKa = 4.97 TTSGTRR207 pKa = 11.84 KK208 pKa = 9.27 PRR210 pKa = 11.84 GLEE213 pKa = 3.42 PRR215 pKa = 11.84 RR216 pKa = 11.84 RR217 pKa = 11.84 KK218 pKa = 10.23 VKK220 pKa = 7.88 TTVVYY225 pKa = 10.22 GRR227 pKa = 11.84 RR228 pKa = 11.84 RR229 pKa = 11.84 SKK231 pKa = 10.5 SRR233 pKa = 11.84 EE234 pKa = 3.53 RR235 pKa = 11.84 RR236 pKa = 11.84 APTPQRR242 pKa = 11.84 AGSPLPRR249 pKa = 11.84 SSSSHH254 pKa = 5.8 HH255 pKa = 6.7 RR256 pKa = 11.84 SPSPRR261 pKa = 11.84 KK262 pKa = 9.39
Molecular weight: 30.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.202
IPC2_protein 9.282
IPC_protein 9.531
Toseland 9.999
ProMoST 9.78
Dawson 10.218
Bjellqvist 9.911
Wikipedia 10.409
Rodwell 10.452
Grimsley 10.292
Solomon 10.262
Lehninger 10.218
Nozaki 9.984
DTASelect 9.911
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.131
Patrickios 9.867
IPC_peptide 10.248
IPC2_peptide 8.565
IPC2.peptide.svr19 8.389
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
1
5
1848
167
786
369.6
41.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.223 ± 0.756
1.407 ± 0.269
4.437 ± 0.522
4.221 ± 0.564
3.68 ± 0.44
5.844 ± 0.683
2.814 ± 0.455
5.952 ± 0.469
5.357 ± 0.641
10.877 ± 1.696
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.623 ± 0.268
3.03 ± 0.855
7.251 ± 0.479
4.004 ± 0.559
7.468 ± 1.407
8.766 ± 0.797
6.818 ± 0.429
4.762 ± 0.514
2.543 ± 0.336
2.922 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here