Ruminococcaceae bacterium YAD3003

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; unclassified Oscillospiraceae

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2342 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3CFU3|A0A1H3CFU3_9FIRM Formate--tetrahydrofolate ligase OS=Ruminococcaceae bacterium YAD3003 OX=1520816 GN=fhs PE=3 SV=1
MM1 pKa = 7.4KK2 pKa = 9.79PVKK5 pKa = 9.74TEE7 pKa = 3.9YY8 pKa = 10.34KK9 pKa = 10.13ALAAITAGTIAVLSIAACSIASSNTPDD36 pKa = 3.07TTVPSEE42 pKa = 4.13SEE44 pKa = 4.4TIVEE48 pKa = 4.55TTFSDD53 pKa = 3.34NDD55 pKa = 3.38LNAGYY60 pKa = 10.32DD61 pKa = 3.36EE62 pKa = 5.48GEE64 pKa = 4.05AQIITLNGTSASSDD78 pKa = 3.25SSSVKK83 pKa = 9.98TEE85 pKa = 3.87GSVVTITGKK94 pKa = 8.55GTYY97 pKa = 10.12VITGTLNDD105 pKa = 4.4GYY107 pKa = 10.5IVVDD111 pKa = 4.61AGDD114 pKa = 3.74SDD116 pKa = 5.4DD117 pKa = 4.8IRR119 pKa = 11.84IVLDD123 pKa = 3.45NADD126 pKa = 3.88ITSSDD131 pKa = 3.5YY132 pKa = 11.55AAIYY136 pKa = 9.55CLNADD141 pKa = 3.42NVYY144 pKa = 8.67ITLADD149 pKa = 3.91GSSNSLSSTGEE160 pKa = 3.72FDD162 pKa = 4.67SKK164 pKa = 11.36DD165 pKa = 3.72SNSVDD170 pKa = 3.01GAIFAKK176 pKa = 10.02TDD178 pKa = 2.99ITINGSGTLKK188 pKa = 10.58INSTDD193 pKa = 3.12HH194 pKa = 6.85GIVGKK199 pKa = 10.35DD200 pKa = 3.15DD201 pKa = 3.33VTITGGGISIKK212 pKa = 10.42SSSDD216 pKa = 3.35GIQANDD222 pKa = 3.4SVSVKK227 pKa = 10.48DD228 pKa = 3.43AVINIVCGKK237 pKa = 10.5DD238 pKa = 3.75GIQADD243 pKa = 3.92NDD245 pKa = 3.63DD246 pKa = 5.27DD247 pKa = 3.88KK248 pKa = 11.65TKK250 pKa = 10.61GYY252 pKa = 11.04VYY254 pKa = 9.97IASGSITISAGDD266 pKa = 3.87DD267 pKa = 4.09GITASSSLQIDD278 pKa = 4.49DD279 pKa = 3.73GTIDD283 pKa = 2.97ITGSYY288 pKa = 9.77EE289 pKa = 4.0GLEE292 pKa = 4.04GRR294 pKa = 11.84YY295 pKa = 7.28VTVNGGTISIVSSDD309 pKa = 3.68DD310 pKa = 3.9GINAVSSTSSGEE322 pKa = 3.75MFQADD327 pKa = 4.48DD328 pKa = 4.75SKK330 pKa = 11.75LCINGGEE337 pKa = 4.36IYY339 pKa = 11.12VNTQGDD345 pKa = 4.42GVDD348 pKa = 3.57SNGTFEE354 pKa = 4.18MTGGILTVMGPTSGANGSLDD374 pKa = 3.73VNGSAVISGGTVVMAGASGMATNFTDD400 pKa = 3.6ATQGTALLTVGNQSEE415 pKa = 4.43GSEE418 pKa = 3.94ITVTDD423 pKa = 3.21STGNVILSATSDD435 pKa = 3.37CSYY438 pKa = 9.97QTVLVSSPDD447 pKa = 3.43MEE449 pKa = 5.76KK450 pKa = 10.95DD451 pKa = 2.94GTYY454 pKa = 9.98TVTAGSYY461 pKa = 10.62SEE463 pKa = 4.73TITLSGYY470 pKa = 10.79LYY472 pKa = 10.72GAGGGFGGGVPGGDD486 pKa = 3.87PGQMPAGNSGDD497 pKa = 3.92FPSGGPGGMHH507 pKa = 7.1PPFF510 pKa = 5.38

Molecular weight: 51.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3CDP6|A0A1H3CDP6_9FIRM 50S ribosomal protein L16 OS=Ruminococcaceae bacterium YAD3003 OX=1520816 GN=rplP PE=3 SV=1
MM1 pKa = 7.7AKK3 pKa = 8.05MTFQPKK9 pKa = 9.04KK10 pKa = 7.6RR11 pKa = 11.84QRR13 pKa = 11.84AKK15 pKa = 9.44VHH17 pKa = 5.51GFRR20 pKa = 11.84ARR22 pKa = 11.84MEE24 pKa = 4.12TANGRR29 pKa = 11.84KK30 pKa = 9.05VLAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.87GRR40 pKa = 11.84KK41 pKa = 8.75RR42 pKa = 11.84LAVV45 pKa = 3.41

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2342

0

2342

814396

39

1746

347.7

38.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.516 ± 0.054

1.57 ± 0.019

6.497 ± 0.045

6.971 ± 0.05

4.527 ± 0.039

7.03 ± 0.045

1.516 ± 0.021

7.708 ± 0.043

6.648 ± 0.038

8.398 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.831 ± 0.023

4.584 ± 0.031

3.542 ± 0.038

2.395 ± 0.021

4.031 ± 0.033

6.425 ± 0.034

5.599 ± 0.041

7.071 ± 0.037

0.865 ± 0.017

4.275 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski