Pseudomonas phage DL54

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Bruynoghevirus; unclassified Bruynoghevirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6WDD2|A0A0F6WDD2_9CAUD GGACT domain-containing protein OS=Pseudomonas phage DL54 OX=1640969 PE=4 SV=1
MM1 pKa = 7.53ASKK4 pKa = 10.65KK5 pKa = 10.38EE6 pKa = 4.02SLEE9 pKa = 3.98DD10 pKa = 3.39QARR13 pKa = 11.84KK14 pKa = 9.69EE15 pKa = 3.76IALEE19 pKa = 4.25KK20 pKa = 10.13EE21 pKa = 4.86FSGSWGGPEE30 pKa = 4.13IDD32 pKa = 5.3ADD34 pKa = 4.14DD35 pKa = 4.73FPLGSACGLDD45 pKa = 3.54PEE47 pKa = 4.85VCEE50 pKa = 4.52SCQQ53 pKa = 3.52

Molecular weight: 5.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6WD08|A0A0F6WD08_9CAUD Portal (Connector) protein OS=Pseudomonas phage DL54 OX=1640969 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.34SPYY5 pKa = 9.36EE6 pKa = 4.1AAHH9 pKa = 6.38EE10 pKa = 3.86RR11 pKa = 11.84AQMINRR17 pKa = 11.84LKK19 pKa = 10.78KK20 pKa = 7.41LTRR23 pKa = 11.84MIRR26 pKa = 11.84VHH28 pKa = 7.21PDD30 pKa = 2.8PRR32 pKa = 11.84WIVEE36 pKa = 3.95RR37 pKa = 11.84QEE39 pKa = 4.88LIRR42 pKa = 11.84KK43 pKa = 8.89LSKK46 pKa = 10.75

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

14010

42

1055

197.3

22.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.373 ± 0.52

1.035 ± 0.153

5.96 ± 0.21

7.173 ± 0.41

3.754 ± 0.217

8.244 ± 0.33

2.006 ± 0.202

5.36 ± 0.209

5.839 ± 0.341

8.137 ± 0.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.175

4.325 ± 0.23

4.254 ± 0.233

4.047 ± 0.278

6.003 ± 0.347

5.76 ± 0.274

5.061 ± 0.283

7.052 ± 0.238

1.67 ± 0.145

3.241 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski