Tomato leaf curl Madagascar virus-Menabe [Madagascar:Morondova:2001]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato leaf curl Madagascar virus

Average proteome isoelectric point is 7.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5DVC7|Q5DVC7_9GEMI Transcriptional activator protein OS=Tomato leaf curl Madagascar virus-Menabe [Madagascar:Morondova:2001] OX=996311 PE=3 SV=1
MM1 pKa = 7.67APLRR5 pKa = 11.84RR6 pKa = 11.84FKK8 pKa = 10.4ICAKK12 pKa = 10.36NYY14 pKa = 8.64FLTYY18 pKa = 9.06PKK20 pKa = 10.53CSLTKK25 pKa = 10.68EE26 pKa = 4.19EE27 pKa = 4.68TLSQIQSLQTPVNKK41 pKa = 10.17LFIKK45 pKa = 10.04ICRR48 pKa = 11.84EE49 pKa = 3.52LHH51 pKa = 6.26EE52 pKa = 4.93NGEE55 pKa = 4.28PHH57 pKa = 6.66LHH59 pKa = 6.03VLIQFEE65 pKa = 4.74GKK67 pKa = 8.8YY68 pKa = 9.53QCTNQRR74 pKa = 11.84FFDD77 pKa = 4.07LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.8KK106 pKa = 11.28DD107 pKa = 3.26GDD109 pKa = 3.83TIEE112 pKa = 4.07WGEE115 pKa = 3.76FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQSANDD132 pKa = 3.81AYY134 pKa = 10.71AAALNAGSKK143 pKa = 10.29SEE145 pKa = 3.84ALRR148 pKa = 11.84VIKK151 pKa = 10.2EE152 pKa = 3.88LAPKK156 pKa = 10.6DD157 pKa = 3.7FVLQFHH163 pKa = 6.68NLNSNLDD170 pKa = 4.08RR171 pKa = 11.84IFQEE175 pKa = 4.24PPAPYY180 pKa = 9.99VSPFLSSSFNQVPEE194 pKa = 4.2EE195 pKa = 3.99LEE197 pKa = 3.93EE198 pKa = 4.22WVSEE202 pKa = 4.09NVMDD206 pKa = 5.26AAARR210 pKa = 11.84PWRR213 pKa = 11.84PNSIVIEE220 pKa = 4.34GDD222 pKa = 3.08SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.26YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTSSYY311 pKa = 11.31KK312 pKa = 10.61EE313 pKa = 3.92FLDD316 pKa = 3.67EE317 pKa = 4.54EE318 pKa = 4.35RR319 pKa = 11.84NAALKK324 pKa = 9.9QWAIKK329 pKa = 10.25NATFVTLHH337 pKa = 6.42EE338 pKa = 4.54PLFTSANQGPTPYY351 pKa = 10.08SEE353 pKa = 4.91EE354 pKa = 4.18EE355 pKa = 3.81NHH357 pKa = 5.97TAA359 pKa = 4.8

Molecular weight: 40.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5DVD0|Q5DVD0_9GEMI Protein V2 OS=Tomato leaf curl Madagascar virus-Menabe [Madagascar:Morondova:2001] OX=996311 GN=AV2 PE=3 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.44RR4 pKa = 11.84PGDD7 pKa = 3.95IIISTPVSKK16 pKa = 10.56VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.03SPYY27 pKa = 7.84TNRR30 pKa = 11.84VAVPIAPATNRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84SWTYY47 pKa = 9.26RR48 pKa = 11.84PMYY51 pKa = 9.54RR52 pKa = 11.84KK53 pKa = 8.92PRR55 pKa = 11.84MYY57 pKa = 10.82RR58 pKa = 11.84MYY60 pKa = 10.57RR61 pKa = 11.84SPDD64 pKa = 3.29VPKK67 pKa = 10.81GCEE70 pKa = 4.39GPCKK74 pKa = 10.23VQSFEE79 pKa = 3.87QRR81 pKa = 11.84DD82 pKa = 4.06DD83 pKa = 3.76VKK85 pKa = 10.08HH86 pKa = 5.63TGIARR91 pKa = 11.84CVSDD95 pKa = 3.31VTRR98 pKa = 11.84GSGITHH104 pKa = 6.35RR105 pKa = 11.84VGKK108 pKa = 9.68RR109 pKa = 11.84FCIKK113 pKa = 10.26SIYY116 pKa = 9.95ILGKK120 pKa = 9.21VWMDD124 pKa = 3.5EE125 pKa = 4.15NIKK128 pKa = 10.08KK129 pKa = 9.99QNHH132 pKa = 5.07TNNVMFFLVRR142 pKa = 11.84DD143 pKa = 3.89RR144 pKa = 11.84RR145 pKa = 11.84PSGSAPMDD153 pKa = 4.08FGQVFNMFDD162 pKa = 3.99NEE164 pKa = 4.11PSTATVKK171 pKa = 10.86NDD173 pKa = 2.97LRR175 pKa = 11.84DD176 pKa = 3.48RR177 pKa = 11.84LQVLRR182 pKa = 11.84KK183 pKa = 8.55FHH185 pKa = 5.96ATVVGGPSGMKK196 pKa = 9.47EE197 pKa = 3.54QALVKK202 pKa = 10.22RR203 pKa = 11.84FYY205 pKa = 10.61RR206 pKa = 11.84INNHH210 pKa = 4.52VVYY213 pKa = 10.43NHH215 pKa = 5.84QEE217 pKa = 3.45AAKK220 pKa = 10.4YY221 pKa = 9.25EE222 pKa = 4.13NHH224 pKa = 6.46TEE226 pKa = 3.99NALLLYY232 pKa = 7.28MACTHH237 pKa = 6.05YY238 pKa = 11.18SNPVYY243 pKa = 9.91ATLKK247 pKa = 9.47IRR249 pKa = 11.84IYY251 pKa = 10.65FYY253 pKa = 11.26DD254 pKa = 3.34AVTNN258 pKa = 4.16

Molecular weight: 29.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1102

100

359

183.7

21.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.354 ± 1.018

2.087 ± 0.461

5.082 ± 0.424

4.9 ± 0.822

4.356 ± 0.575

4.9 ± 0.401

4.265 ± 0.895

5.717 ± 0.978

5.354 ± 0.704

7.441 ± 0.967

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.269 ± 0.53

5.898 ± 0.875

6.352 ± 0.586

5.172 ± 0.859

7.169 ± 1.099

7.26 ± 1.039

5.626 ± 0.793

5.808 ± 1.003

1.361 ± 0.221

3.63 ± 0.595
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski