Bacillus phage BeachBum

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Harambevirus; Bacillus virus BeachBum

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9SGK8|A0A1X9SGK8_9CAUD DNA encapsidation protein OS=Bacillus phage BeachBum OX=1983461 GN=BEACHBUM_24 PE=4 SV=1
MM1 pKa = 7.49NNHH4 pKa = 5.7FMSIGRR10 pKa = 11.84NCSKK14 pKa = 10.7CNNVRR19 pKa = 11.84LNEE22 pKa = 4.37DD23 pKa = 3.4FSKK26 pKa = 11.06SEE28 pKa = 4.39EE29 pKa = 3.97ICNEE33 pKa = 3.81CFDD36 pKa = 3.85KK37 pKa = 11.23EE38 pKa = 4.14EE39 pKa = 4.81RR40 pKa = 11.84FVDD43 pKa = 4.32SYY45 pKa = 9.87TLIYY49 pKa = 9.08ITEE52 pKa = 4.53DD53 pKa = 3.03EE54 pKa = 4.19TGKK57 pKa = 10.42RR58 pKa = 11.84FEE60 pKa = 6.05AILEE64 pKa = 4.11NQTIEE69 pKa = 4.12EE70 pKa = 4.33TEE72 pKa = 3.96IIYY75 pKa = 10.85GNIIDD80 pKa = 6.08KK81 pKa = 10.72IIVWNVILTMM91 pKa = 4.62

Molecular weight: 10.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9SGT8|A0A1X9SGT8_9CAUD DNA polymerase OS=Bacillus phage BeachBum OX=1983461 GN=BEACHBUM_5 PE=3 SV=1
MM1 pKa = 7.36NKK3 pKa = 9.77YY4 pKa = 9.84KK5 pKa = 10.75RR6 pKa = 11.84LAEE9 pKa = 4.54RR10 pKa = 11.84INVRR14 pKa = 11.84WNGSKK19 pKa = 10.71CSTGWAHH26 pKa = 7.43DD27 pKa = 4.23MIDD30 pKa = 4.33AITILMMKK38 pKa = 8.78EE39 pKa = 3.61QNKK42 pKa = 10.0

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30

0

30

6387

42

728

212.9

24.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.12 ± 0.438

0.83 ± 0.177

6.169 ± 0.27

8.22 ± 0.638

4.462 ± 0.341

6.404 ± 0.453

1.879 ± 0.201

6.905 ± 0.426

8.596 ± 0.454

6.685 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.163 ± 0.233

6.748 ± 0.364

2.881 ± 0.286

3.695 ± 0.32

4.212 ± 0.295

5.355 ± 0.293

6.764 ± 0.528

6.09 ± 0.41

1.143 ± 0.157

4.681 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski