Escherichia phage G AB-2017

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; unclassified Kagunavirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q1PUP5|A0A1Q1PUP5_9CAUD Uncharacterized protein OS=Escherichia phage G AB-2017 OX=1933113 GN=G_65 PE=4 SV=1
MM1 pKa = 7.07FVIMYY6 pKa = 10.06SIGEE10 pKa = 4.09EE11 pKa = 4.33EE12 pKa = 4.65YY13 pKa = 10.13EE14 pKa = 3.93HH15 pKa = 7.18SRR17 pKa = 11.84YY18 pKa = 8.27LTSAEE23 pKa = 4.11AEE25 pKa = 4.23NMIHH29 pKa = 6.88EE30 pKa = 5.17LDD32 pKa = 4.34ADD34 pKa = 3.84SFDD37 pKa = 3.96YY38 pKa = 11.13AWIEE42 pKa = 4.26EE43 pKa = 4.2IAEE46 pKa = 4.03

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q1PUR5|A0A1Q1PUR5_9CAUD Uncharacterized protein OS=Escherichia phage G AB-2017 OX=1933113 GN=G_62 PE=4 SV=1
MM1 pKa = 7.08STIQEE6 pKa = 4.05LHH8 pKa = 6.26KK9 pKa = 10.95SYY11 pKa = 10.6LQQHH15 pKa = 6.22RR16 pKa = 11.84NGAKK20 pKa = 10.14LGRR23 pKa = 11.84SKK25 pKa = 9.94TIYY28 pKa = 9.54RR29 pKa = 11.84VFRR32 pKa = 11.84MVIRR36 pKa = 11.84DD37 pKa = 3.59EE38 pKa = 4.0SAKK41 pKa = 9.94IIRR44 pKa = 11.84MTKK47 pKa = 8.25EE48 pKa = 3.62TFKK51 pKa = 11.3

Molecular weight: 6.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12575

42

827

184.9

20.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.781 ± 0.573

1.105 ± 0.14

6.266 ± 0.236

6.958 ± 0.432

3.817 ± 0.175

7.571 ± 0.267

1.734 ± 0.198

5.368 ± 0.16

6.02 ± 0.358

7.507 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.212

4.398 ± 0.271

3.658 ± 0.219

3.905 ± 0.339

5.304 ± 0.236

5.765 ± 0.333

6.203 ± 0.385

7.101 ± 0.275

1.408 ± 0.173

3.507 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski