Halomonas sp. TQ8S

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4010 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A368U602|A0A368U602_9GAMM NAD-dependent protein deacetylase OS=Halomonas sp. TQ8S OX=2282306 GN=DU506_07180 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 9.06KK3 pKa = 8.27TLLATAIAGAAVMAASGAQAATVYY27 pKa = 11.04NEE29 pKa = 5.19DD30 pKa = 3.42GTKK33 pKa = 10.4LDD35 pKa = 4.25LYY37 pKa = 11.47GNIQIAYY44 pKa = 9.61LNQTQADD51 pKa = 4.02GSDD54 pKa = 3.4EE55 pKa = 6.07DD56 pKa = 3.91EE57 pKa = 5.0LKK59 pKa = 11.28DD60 pKa = 3.5NGSTIGFAGEE70 pKa = 3.88HH71 pKa = 6.43MITRR75 pKa = 11.84DD76 pKa = 3.02LSGYY80 pKa = 9.41FKK82 pKa = 11.41AEE84 pKa = 4.04FEE86 pKa = 4.32HH87 pKa = 7.35DD88 pKa = 3.3ADD90 pKa = 3.95EE91 pKa = 4.67AKK93 pKa = 9.81TAGGLNTGDD102 pKa = 3.17QAYY105 pKa = 9.77IGLKK109 pKa = 10.68GNFGDD114 pKa = 4.2VRR116 pKa = 11.84LGSWDD121 pKa = 3.9PLIDD125 pKa = 5.15DD126 pKa = 5.77WIQDD130 pKa = 3.93PITNNEE136 pKa = 4.08AFDD139 pKa = 3.84VTDD142 pKa = 3.44TNSRR146 pKa = 11.84IVGIEE151 pKa = 3.6NTRR154 pKa = 11.84EE155 pKa = 3.81GDD157 pKa = 3.39KK158 pKa = 10.66LTFTSASLNGLQFAVGTQFEE178 pKa = 4.67GDD180 pKa = 3.67AEE182 pKa = 4.58SDD184 pKa = 3.14GSAVVNPPVGGSLSVSQDD202 pKa = 3.0SGSNASFFGGVKK214 pKa = 8.36YY215 pKa = 8.6TAGAFSVAAVVDD227 pKa = 4.06DD228 pKa = 4.97LGNNDD233 pKa = 3.37GTFTGTDD240 pKa = 3.12ADD242 pKa = 4.25GNTIGGDD249 pKa = 3.46FDD251 pKa = 6.23AGVQYY256 pKa = 11.01GITGQYY262 pKa = 9.02TVDD265 pKa = 3.55TLRR268 pKa = 11.84VALKK272 pKa = 10.6LEE274 pKa = 4.26RR275 pKa = 11.84FEE277 pKa = 5.22SDD279 pKa = 2.44NDD281 pKa = 3.62YY282 pKa = 11.53VADD285 pKa = 3.74TNYY288 pKa = 10.42YY289 pKa = 10.53GLGARR294 pKa = 11.84YY295 pKa = 9.52GYY297 pKa = 10.94GVGDD301 pKa = 3.59IYY303 pKa = 11.28GAYY306 pKa = 9.94QYY308 pKa = 11.63VDD310 pKa = 3.13VGGGNFVDD318 pKa = 4.39TAQDD322 pKa = 3.25AAFSGDD328 pKa = 3.21WPNEE332 pKa = 4.1RR333 pKa = 11.84EE334 pKa = 4.02DD335 pKa = 3.51EE336 pKa = 4.43SYY338 pKa = 11.73NEE340 pKa = 3.95IVLGATYY347 pKa = 10.49NISDD351 pKa = 3.39SMYY354 pKa = 9.79TFIEE358 pKa = 4.21GAMYY362 pKa = 10.79DD363 pKa = 3.62RR364 pKa = 11.84TNDD367 pKa = 3.29EE368 pKa = 4.45DD369 pKa = 5.05DD370 pKa = 4.01GVAVGAVYY378 pKa = 10.44LFF380 pKa = 4.21

Molecular weight: 40.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A368U5U8|A0A368U5U8_9GAMM Histidine kinase OS=Halomonas sp. TQ8S OX=2282306 GN=DU506_07310 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.17NGRR28 pKa = 11.84AILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4010

0

4010

1279303

30

3406

319.0

35.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.485 ± 0.046

0.967 ± 0.014

5.591 ± 0.027

6.534 ± 0.035

3.491 ± 0.024

7.622 ± 0.034

2.541 ± 0.019

4.909 ± 0.025

3.334 ± 0.036

11.224 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.021

2.908 ± 0.021

4.839 ± 0.027

4.354 ± 0.032

6.809 ± 0.037

5.738 ± 0.025

5.147 ± 0.026

6.972 ± 0.033

1.485 ± 0.019

2.461 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski