Circoviridae sp.
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385E449|A0A385E449_9CIRC ATP-dependent helicase Rep OS=Circoviridae sp. OX=1954248 PE=3 SV=1
MM1 pKa = 7.19 AQAPKK6 pKa = 9.39 VRR8 pKa = 11.84 HH9 pKa = 4.83 WVFTLNNYY17 pKa = 7.04 TAVDD21 pKa = 3.43 EE22 pKa = 5.19 SEE24 pKa = 4.08 ILKK27 pKa = 10.69 CNYY30 pKa = 9.59 KK31 pKa = 10.32 SLGYY35 pKa = 9.69 GKK37 pKa = 10.16 EE38 pKa = 4.09 VGEE41 pKa = 4.62 SGTPHH46 pKa = 5.66 LQGWIAFHH54 pKa = 6.25 NPQRR58 pKa = 11.84 LSALKK63 pKa = 9.96 KK64 pKa = 8.22 IHH66 pKa = 6.92 ARR68 pKa = 11.84 AHH70 pKa = 5.44 WEE72 pKa = 3.9 PMKK75 pKa = 10.61 GTLEE79 pKa = 4.67 DD80 pKa = 3.39 SRR82 pKa = 11.84 EE83 pKa = 3.99 YY84 pKa = 10.64 CKK86 pKa = 10.64 KK87 pKa = 10.75 DD88 pKa = 2.77 GDD90 pKa = 4.05 YY91 pKa = 11.72 VEE93 pKa = 5.63 FGDD96 pKa = 4.18 ILEE99 pKa = 4.34 MQRR102 pKa = 11.84 KK103 pKa = 8.55 RR104 pKa = 11.84 RR105 pKa = 11.84 EE106 pKa = 3.78 AAEE109 pKa = 4.2 KK110 pKa = 10.28 GGKK113 pKa = 8.94 ANKK116 pKa = 9.19 EE117 pKa = 3.75 RR118 pKa = 11.84 WSKK121 pKa = 10.95 CIALVRR127 pKa = 11.84 DD128 pKa = 3.99 GLAAQALEE136 pKa = 4.33 EE137 pKa = 4.28 FPDD140 pKa = 3.3 IFVRR144 pKa = 11.84 YY145 pKa = 9.13 HH146 pKa = 5.96 KK147 pKa = 10.83 AIDD150 pKa = 3.81 GAIKK154 pKa = 10.11 RR155 pKa = 11.84 CEE157 pKa = 4.15 EE158 pKa = 3.84 KK159 pKa = 10.47 PYY161 pKa = 11.09 ALPEE165 pKa = 4.24 LDD167 pKa = 3.42 NRR169 pKa = 11.84 WYY171 pKa = 10.42 YY172 pKa = 10.89 GPSGTGKK179 pKa = 10.2 SKK181 pKa = 10.31 SARR184 pKa = 11.84 DD185 pKa = 4.18 EE186 pKa = 4.4 FPDD189 pKa = 5.06 AYY191 pKa = 11.23 VKK193 pKa = 10.91 LNNKK197 pKa = 7.82 WWDD200 pKa = 3.91 GYY202 pKa = 11.17 DD203 pKa = 3.76 GEE205 pKa = 4.77 DD206 pKa = 3.4 TVIIDD211 pKa = 5.83 DD212 pKa = 4.05 LDD214 pKa = 5.04 KK215 pKa = 11.32 YY216 pKa = 11.41 DD217 pKa = 3.87 KK218 pKa = 11.45 ALGGDD223 pKa = 4.07 LKK225 pKa = 10.5 RR226 pKa = 11.84 WSDD229 pKa = 3.73 HH230 pKa = 5.69 YY231 pKa = 10.88 PFPAEE236 pKa = 3.92 YY237 pKa = 9.62 KK238 pKa = 9.72 GGVMVIRR245 pKa = 11.84 PKK247 pKa = 10.64 RR248 pKa = 11.84 VIVTSNYY255 pKa = 9.88 LPEE258 pKa = 5.12 EE259 pKa = 3.97 IWDD262 pKa = 4.14 DD263 pKa = 3.91 EE264 pKa = 4.48 QTTGPINRR272 pKa = 11.84 RR273 pKa = 11.84 FKK275 pKa = 9.71 RR276 pKa = 11.84 TKK278 pKa = 9.87 FDD280 pKa = 3.08 HH281 pKa = 6.96 HH282 pKa = 6.93 VFNPISRR289 pKa = 11.84 PPTPIHH295 pKa = 6.51 EE296 pKa = 5.06 DD297 pKa = 3.46 EE298 pKa = 4.73 FDD300 pKa = 3.95 SLSGIYY306 pKa = 10.18 DD307 pKa = 3.72 LL308 pKa = 6.07
Molecular weight: 35.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.072
IPC2_protein 6.02
IPC_protein 6.109
Toseland 6.504
ProMoST 6.478
Dawson 6.415
Bjellqvist 6.389
Wikipedia 6.427
Rodwell 6.402
Grimsley 6.664
Solomon 6.415
Lehninger 6.415
Nozaki 6.678
DTASelect 6.868
Thurlkill 6.883
EMBOSS 6.854
Sillero 6.781
Patrickios 4.304
IPC_peptide 6.427
IPC2_peptide 6.664
IPC2.peptide.svr19 6.721
Protein with the highest isoelectric point:
>tr|A0A385E449|A0A385E449_9CIRC ATP-dependent helicase Rep OS=Circoviridae sp. OX=1954248 PE=3 SV=1
MM1 pKa = 7.19 AQAPKK6 pKa = 9.39 VRR8 pKa = 11.84 HH9 pKa = 4.83 WVFTLNNYY17 pKa = 7.04 TAVDD21 pKa = 3.43 EE22 pKa = 5.19 SEE24 pKa = 4.08 ILKK27 pKa = 10.69 CNYY30 pKa = 9.59 KK31 pKa = 10.32 SLGYY35 pKa = 9.69 GKK37 pKa = 10.16 EE38 pKa = 4.09 VGEE41 pKa = 4.62 SGTPHH46 pKa = 5.66 LQGWIAFHH54 pKa = 6.25 NPQRR58 pKa = 11.84 LSALKK63 pKa = 9.96 KK64 pKa = 8.22 IHH66 pKa = 6.92 ARR68 pKa = 11.84 AHH70 pKa = 5.44 WEE72 pKa = 3.9 PMKK75 pKa = 10.61 GTLEE79 pKa = 4.67 DD80 pKa = 3.39 SRR82 pKa = 11.84 EE83 pKa = 3.99 YY84 pKa = 10.64 CKK86 pKa = 10.64 KK87 pKa = 10.75 DD88 pKa = 2.77 GDD90 pKa = 4.05 YY91 pKa = 11.72 VEE93 pKa = 5.63 FGDD96 pKa = 4.18 ILEE99 pKa = 4.34 MQRR102 pKa = 11.84 KK103 pKa = 8.55 RR104 pKa = 11.84 RR105 pKa = 11.84 EE106 pKa = 3.78 AAEE109 pKa = 4.2 KK110 pKa = 10.28 GGKK113 pKa = 8.94 ANKK116 pKa = 9.19 EE117 pKa = 3.75 RR118 pKa = 11.84 WSKK121 pKa = 10.95 CIALVRR127 pKa = 11.84 DD128 pKa = 3.99 GLAAQALEE136 pKa = 4.33 EE137 pKa = 4.28 FPDD140 pKa = 3.3 IFVRR144 pKa = 11.84 YY145 pKa = 9.13 HH146 pKa = 5.96 KK147 pKa = 10.83 AIDD150 pKa = 3.81 GAIKK154 pKa = 10.11 RR155 pKa = 11.84 CEE157 pKa = 4.15 EE158 pKa = 3.84 KK159 pKa = 10.47 PYY161 pKa = 11.09 ALPEE165 pKa = 4.24 LDD167 pKa = 3.42 NRR169 pKa = 11.84 WYY171 pKa = 10.42 YY172 pKa = 10.89 GPSGTGKK179 pKa = 10.2 SKK181 pKa = 10.31 SARR184 pKa = 11.84 DD185 pKa = 4.18 EE186 pKa = 4.4 FPDD189 pKa = 5.06 AYY191 pKa = 11.23 VKK193 pKa = 10.91 LNNKK197 pKa = 7.82 WWDD200 pKa = 3.91 GYY202 pKa = 11.17 DD203 pKa = 3.76 GEE205 pKa = 4.77 DD206 pKa = 3.4 TVIIDD211 pKa = 5.83 DD212 pKa = 4.05 LDD214 pKa = 5.04 KK215 pKa = 11.32 YY216 pKa = 11.41 DD217 pKa = 3.87 KK218 pKa = 11.45 ALGGDD223 pKa = 4.07 LKK225 pKa = 10.5 RR226 pKa = 11.84 WSDD229 pKa = 3.73 HH230 pKa = 5.69 YY231 pKa = 10.88 PFPAEE236 pKa = 3.92 YY237 pKa = 9.62 KK238 pKa = 9.72 GGVMVIRR245 pKa = 11.84 PKK247 pKa = 10.64 RR248 pKa = 11.84 VIVTSNYY255 pKa = 9.88 LPEE258 pKa = 5.12 EE259 pKa = 3.97 IWDD262 pKa = 4.14 DD263 pKa = 3.91 EE264 pKa = 4.48 QTTGPINRR272 pKa = 11.84 RR273 pKa = 11.84 FKK275 pKa = 9.71 RR276 pKa = 11.84 TKK278 pKa = 9.87 FDD280 pKa = 3.08 HH281 pKa = 6.96 HH282 pKa = 6.93 VFNPISRR289 pKa = 11.84 PPTPIHH295 pKa = 6.51 EE296 pKa = 5.06 DD297 pKa = 3.46 EE298 pKa = 4.73 FDD300 pKa = 3.95 SLSGIYY306 pKa = 10.18 DD307 pKa = 3.72 LL308 pKa = 6.07
Molecular weight: 35.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.072
IPC2_protein 6.02
IPC_protein 6.109
Toseland 6.504
ProMoST 6.478
Dawson 6.415
Bjellqvist 6.389
Wikipedia 6.427
Rodwell 6.402
Grimsley 6.664
Solomon 6.415
Lehninger 6.415
Nozaki 6.678
DTASelect 6.868
Thurlkill 6.883
EMBOSS 6.854
Sillero 6.781
Patrickios 4.304
IPC_peptide 6.427
IPC2_peptide 6.664
IPC2.peptide.svr19 6.721
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
308
308
308
308.0
35.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.818 ± 0.0
1.299 ± 0.0
8.442 ± 0.0
8.117 ± 0.0
3.571 ± 0.0
7.468 ± 0.0
3.247 ± 0.0
5.519 ± 0.0
9.091 ± 0.0
6.494 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.299 ± 0.0
3.571 ± 0.0
5.844 ± 0.0
1.948 ± 0.0
6.494 ± 0.0
4.545 ± 0.0
3.571 ± 0.0
4.545 ± 0.0
2.922 ± 0.0
5.195 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here