Streptococcus phage Javan181

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A1T6|A0A4D6A1T6_9CAUD Clp protease-like protein OS=Streptococcus phage Javan181 OX=2548029 GN=Javan181_0023 PE=3 SV=1
MM1 pKa = 7.23ATNARR6 pKa = 11.84IGLLTKK12 pKa = 10.43SDD14 pKa = 3.21TATFIKK20 pKa = 10.16VAYY23 pKa = 8.82EE24 pKa = 4.6GYY26 pKa = 9.6PSYY29 pKa = 10.52TGSLLTSAFNSLKK42 pKa = 10.01QVKK45 pKa = 9.39EE46 pKa = 3.78LLKK49 pKa = 10.6KK50 pKa = 10.9GNIIMLEE57 pKa = 4.02EE58 pKa = 4.18NLDD61 pKa = 3.85EE62 pKa = 4.85VEE64 pKa = 4.17TDD66 pKa = 3.5SLVQGTEE73 pKa = 3.79NIRR76 pKa = 11.84FVGSEE81 pKa = 3.81SDD83 pKa = 4.35LEE85 pKa = 4.29GDD87 pKa = 3.73YY88 pKa = 11.27LADD91 pKa = 3.18YY92 pKa = 9.15TYY94 pKa = 11.17VYY96 pKa = 10.93KK97 pKa = 9.97EE98 pKa = 4.33TEE100 pKa = 3.99KK101 pKa = 10.48RR102 pKa = 11.84WYY104 pKa = 9.93ILTDD108 pKa = 3.51GKK110 pKa = 10.84LVPCYY115 pKa = 10.4LL116 pKa = 3.81

Molecular weight: 13.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A1R3|A0A4D6A1R3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan181 OX=2548029 GN=Javan181_0003 PE=4 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84EE3 pKa = 3.79KK4 pKa = 10.68VVEE7 pKa = 3.99QKK9 pKa = 10.73LVSEE13 pKa = 4.44VKK15 pKa = 10.26KK16 pKa = 10.87RR17 pKa = 11.84GGICPKK23 pKa = 9.36WVSPSFGGVPDD34 pKa = 3.93RR35 pKa = 11.84LVFLPKK41 pKa = 10.51GKK43 pKa = 10.42FSMVEE48 pKa = 3.69VKK50 pKa = 10.87APGGKK55 pKa = 8.83PRR57 pKa = 11.84LLQVTRR63 pKa = 11.84HH64 pKa = 5.69RR65 pKa = 11.84LFEE68 pKa = 4.0RR69 pKa = 11.84LGFKK73 pKa = 10.05VHH75 pKa = 6.05VLDD78 pKa = 4.78SIEE81 pKa = 4.64KK82 pKa = 8.99IGEE85 pKa = 4.04VLDD88 pKa = 4.85EE89 pKa = 4.7IEE91 pKa = 4.25FTT93 pKa = 4.14

Molecular weight: 10.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

10257

37

1039

277.2

31.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.117 ± 0.486

0.76 ± 0.12

6.132 ± 0.426

7.263 ± 0.365

3.783 ± 0.233

6.883 ± 0.347

1.872 ± 0.163

5.976 ± 0.318

7.156 ± 0.25

9.467 ± 0.3

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.169

4.514 ± 0.259

3.032 ± 0.24

4.075 ± 0.241

4.027 ± 0.306

6.825 ± 0.506

6.981 ± 0.545

6.834 ± 0.267

1.365 ± 0.134

3.607 ± 0.32
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski