Aliiglaciecola sp. M165
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4070 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A553L388|A0A553L388_9ALTE 30S ribosomal protein S10 OS=Aliiglaciecola sp. M165 OX=2593649 GN=rpsJ PE=3 SV=1
MM1 pKa = 7.65 KK2 pKa = 9.13 ATSIKK7 pKa = 9.98 TPLSLLALALSTAILSGCGSDD28 pKa = 4.59 SEE30 pKa = 5.28 SIPDD34 pKa = 3.94 PVAPPPPPPPPAATDD49 pKa = 4.29 FPAQQASPARR59 pKa = 11.84 FVDD62 pKa = 3.62 GDD64 pKa = 3.6 VGEE67 pKa = 4.54 VFTTEE72 pKa = 3.76 TGLTLYY78 pKa = 10.92 YY79 pKa = 10.42 FLNDD83 pKa = 3.13 EE84 pKa = 4.99 AGSSNCNGVEE94 pKa = 3.74 GDD96 pKa = 4.23 APGSTTDD103 pKa = 3.34 EE104 pKa = 4.38 TSCAGVWPPLLAATGAQPTGSFSTVEE130 pKa = 4.1 RR131 pKa = 11.84 DD132 pKa = 4.19 DD133 pKa = 4.88 GTLQWAYY140 pKa = 11.07 RR141 pKa = 11.84 DD142 pKa = 3.81 FPLYY146 pKa = 10.38 TFSNDD151 pKa = 3.03 SAQGDD156 pKa = 4.19 TNGEE160 pKa = 4.35 GINDD164 pKa = 3.31 VWFVSRR170 pKa = 11.84 PGPSKK175 pKa = 10.34 IADD178 pKa = 3.66 VNSVPSYY185 pKa = 10.34 VGNGTISSVTSTAEE199 pKa = 3.65 VLEE202 pKa = 4.18 SVRR205 pKa = 11.84 LDD207 pKa = 3.29 KK208 pKa = 11.39 EE209 pKa = 4.43 GFSLYY214 pKa = 10.35 IFDD217 pKa = 5.97 NDD219 pKa = 4.64 GLDD222 pKa = 3.28 QSACYY227 pKa = 9.84 GLNGDD232 pKa = 4.27 GCITAWPPLIADD244 pKa = 4.04 NAAKK248 pKa = 9.61 PAAPLSVVEE257 pKa = 4.64 LDD259 pKa = 3.13 NGQMQWAYY267 pKa = 10.36 RR268 pKa = 11.84 GKK270 pKa = 9.45 PLYY273 pKa = 10.44 FFASDD278 pKa = 3.42 TQAGDD283 pKa = 3.5 FNGDD287 pKa = 3.01 GVGGVWHH294 pKa = 7.25 LASQLPAIQRR304 pKa = 11.84 EE305 pKa = 4.4 VNAQSRR311 pKa = 11.84 LSATGRR317 pKa = 11.84 VYY319 pKa = 11.29 ALLPNTDD326 pKa = 3.69 NNNEE330 pKa = 4.16 LEE332 pKa = 4.24 SQQVDD337 pKa = 2.83 KK338 pKa = 11.53 DD339 pKa = 3.49 QFTVYY344 pKa = 10.37 TFDD347 pKa = 3.79 NDD349 pKa = 3.63 TAGTSNCNGDD359 pKa = 4.72 CAVAWPPFLAPDD371 pKa = 4.17 AEE373 pKa = 4.19 PAVGNFSKK381 pKa = 10.62 IEE383 pKa = 4.06 RR384 pKa = 11.84 TDD386 pKa = 3.88 GAIQWAYY393 pKa = 10.72 NDD395 pKa = 3.49 SPLYY399 pKa = 10.48 FFSGDD404 pKa = 3.37 NAKK407 pKa = 9.41 TDD409 pKa = 3.99 LNGDD413 pKa = 3.61 GVGGVWHH420 pKa = 6.68 IVEE423 pKa = 4.73 PPAAPVVSPTSISALSNSLGQTIKK447 pKa = 10.8 VDD449 pKa = 3.91 GEE451 pKa = 4.22 VLALITDD458 pKa = 3.64 NQGNSEE464 pKa = 4.4 AVTEE468 pKa = 4.37 DD469 pKa = 2.97 RR470 pKa = 11.84 TDD472 pKa = 3.67 FQLYY476 pKa = 8.49 TFDD479 pKa = 4.15 NDD481 pKa = 3.28 GDD483 pKa = 4.0 EE484 pKa = 5.1 LSNCTSTGCMQNWPALLANEE504 pKa = 5.06 ADD506 pKa = 3.73 TATAPFSIFEE516 pKa = 4.28 RR517 pKa = 11.84 ADD519 pKa = 3.22 GHH521 pKa = 5.76 NQWAFNGQPLYY532 pKa = 10.85 FFTGDD537 pKa = 3.23 TSAGTTNGEE546 pKa = 4.15 GVGDD550 pKa = 3.65 VWFVARR556 pKa = 11.84 PAPVRR561 pKa = 11.84 VFNSTDD567 pKa = 3.33 DD568 pKa = 3.59 GLMIVANDD576 pKa = 4.15 LVLASQGKK584 pKa = 6.8 TAEE587 pKa = 4.07 QLNDD591 pKa = 3.17 LTLYY595 pKa = 10.48 TFDD598 pKa = 6.18 DD599 pKa = 3.84 DD600 pKa = 4.25 TANSGEE606 pKa = 4.37 STCFGGCAVTWPPLYY621 pKa = 9.94 ATSADD626 pKa = 3.66 QAFGEE631 pKa = 4.38 YY632 pKa = 10.12 EE633 pKa = 3.93 IISRR637 pKa = 11.84 TEE639 pKa = 3.7 SDD641 pKa = 3.89 SSQTLQWTYY650 pKa = 11.33 KK651 pKa = 10.45 GLPLYY656 pKa = 10.09 FFISDD661 pKa = 4.16 SEE663 pKa = 4.47 LGDD666 pKa = 3.39 TGGDD670 pKa = 3.62 YY671 pKa = 7.59 PTWEE675 pKa = 4.0 IARR678 pKa = 11.84 PP679 pKa = 3.51
Molecular weight: 72.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.694
IPC_protein 3.745
Toseland 3.503
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.414
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.113
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.859
Patrickios 1.024
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.775
Protein with the highest isoelectric point:
>tr|A0A553KWG5|A0A553KWG5_9ALTE Monovalent cation/H+ antiporter subunit D OS=Aliiglaciecola sp. M165 OX=2593649 GN=FM019_19940 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 9.13 RR14 pKa = 11.84 SHH16 pKa = 6.17 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.2 VLANRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.38 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4070
0
4070
1360031
17
3453
334.2
37.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.319 ± 0.04
0.971 ± 0.013
5.821 ± 0.035
6.155 ± 0.036
4.48 ± 0.026
6.661 ± 0.039
2.219 ± 0.02
6.4 ± 0.026
5.293 ± 0.038
10.193 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.485 ± 0.02
4.609 ± 0.028
3.857 ± 0.024
4.814 ± 0.039
4.42 ± 0.027
6.852 ± 0.034
5.241 ± 0.03
6.945 ± 0.031
1.257 ± 0.016
3.007 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here