Aliiglaciecola sp. M165

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Aliiglaciecola; unclassified Aliiglaciecola

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4070 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A553L388|A0A553L388_9ALTE 30S ribosomal protein S10 OS=Aliiglaciecola sp. M165 OX=2593649 GN=rpsJ PE=3 SV=1
MM1 pKa = 7.65KK2 pKa = 9.13ATSIKK7 pKa = 9.98TPLSLLALALSTAILSGCGSDD28 pKa = 4.59SEE30 pKa = 5.28SIPDD34 pKa = 3.94PVAPPPPPPPPAATDD49 pKa = 4.29FPAQQASPARR59 pKa = 11.84FVDD62 pKa = 3.62GDD64 pKa = 3.6VGEE67 pKa = 4.54VFTTEE72 pKa = 3.76TGLTLYY78 pKa = 10.92YY79 pKa = 10.42FLNDD83 pKa = 3.13EE84 pKa = 4.99AGSSNCNGVEE94 pKa = 3.74GDD96 pKa = 4.23APGSTTDD103 pKa = 3.34EE104 pKa = 4.38TSCAGVWPPLLAATGAQPTGSFSTVEE130 pKa = 4.1RR131 pKa = 11.84DD132 pKa = 4.19DD133 pKa = 4.88GTLQWAYY140 pKa = 11.07RR141 pKa = 11.84DD142 pKa = 3.81FPLYY146 pKa = 10.38TFSNDD151 pKa = 3.03SAQGDD156 pKa = 4.19TNGEE160 pKa = 4.35GINDD164 pKa = 3.31VWFVSRR170 pKa = 11.84PGPSKK175 pKa = 10.34IADD178 pKa = 3.66VNSVPSYY185 pKa = 10.34VGNGTISSVTSTAEE199 pKa = 3.65VLEE202 pKa = 4.18SVRR205 pKa = 11.84LDD207 pKa = 3.29KK208 pKa = 11.39EE209 pKa = 4.43GFSLYY214 pKa = 10.35IFDD217 pKa = 5.97NDD219 pKa = 4.64GLDD222 pKa = 3.28QSACYY227 pKa = 9.84GLNGDD232 pKa = 4.27GCITAWPPLIADD244 pKa = 4.04NAAKK248 pKa = 9.61PAAPLSVVEE257 pKa = 4.64LDD259 pKa = 3.13NGQMQWAYY267 pKa = 10.36RR268 pKa = 11.84GKK270 pKa = 9.45PLYY273 pKa = 10.44FFASDD278 pKa = 3.42TQAGDD283 pKa = 3.5FNGDD287 pKa = 3.01GVGGVWHH294 pKa = 7.25LASQLPAIQRR304 pKa = 11.84EE305 pKa = 4.4VNAQSRR311 pKa = 11.84LSATGRR317 pKa = 11.84VYY319 pKa = 11.29ALLPNTDD326 pKa = 3.69NNNEE330 pKa = 4.16LEE332 pKa = 4.24SQQVDD337 pKa = 2.83KK338 pKa = 11.53DD339 pKa = 3.49QFTVYY344 pKa = 10.37TFDD347 pKa = 3.79NDD349 pKa = 3.63TAGTSNCNGDD359 pKa = 4.72CAVAWPPFLAPDD371 pKa = 4.17AEE373 pKa = 4.19PAVGNFSKK381 pKa = 10.62IEE383 pKa = 4.06RR384 pKa = 11.84TDD386 pKa = 3.88GAIQWAYY393 pKa = 10.72NDD395 pKa = 3.49SPLYY399 pKa = 10.48FFSGDD404 pKa = 3.37NAKK407 pKa = 9.41TDD409 pKa = 3.99LNGDD413 pKa = 3.61GVGGVWHH420 pKa = 6.68IVEE423 pKa = 4.73PPAAPVVSPTSISALSNSLGQTIKK447 pKa = 10.8VDD449 pKa = 3.91GEE451 pKa = 4.22VLALITDD458 pKa = 3.64NQGNSEE464 pKa = 4.4AVTEE468 pKa = 4.37DD469 pKa = 2.97RR470 pKa = 11.84TDD472 pKa = 3.67FQLYY476 pKa = 8.49TFDD479 pKa = 4.15NDD481 pKa = 3.28GDD483 pKa = 4.0EE484 pKa = 5.1LSNCTSTGCMQNWPALLANEE504 pKa = 5.06ADD506 pKa = 3.73TATAPFSIFEE516 pKa = 4.28RR517 pKa = 11.84ADD519 pKa = 3.22GHH521 pKa = 5.76NQWAFNGQPLYY532 pKa = 10.85FFTGDD537 pKa = 3.23TSAGTTNGEE546 pKa = 4.15GVGDD550 pKa = 3.65VWFVARR556 pKa = 11.84PAPVRR561 pKa = 11.84VFNSTDD567 pKa = 3.33DD568 pKa = 3.59GLMIVANDD576 pKa = 4.15LVLASQGKK584 pKa = 6.8TAEE587 pKa = 4.07QLNDD591 pKa = 3.17LTLYY595 pKa = 10.48TFDD598 pKa = 6.18DD599 pKa = 3.84DD600 pKa = 4.25TANSGEE606 pKa = 4.37STCFGGCAVTWPPLYY621 pKa = 9.94ATSADD626 pKa = 3.66QAFGEE631 pKa = 4.38YY632 pKa = 10.12EE633 pKa = 3.93IISRR637 pKa = 11.84TEE639 pKa = 3.7SDD641 pKa = 3.89SSQTLQWTYY650 pKa = 11.33KK651 pKa = 10.45GLPLYY656 pKa = 10.09FFISDD661 pKa = 4.16SEE663 pKa = 4.47LGDD666 pKa = 3.39TGGDD670 pKa = 3.62YY671 pKa = 7.59PTWEE675 pKa = 4.0IARR678 pKa = 11.84PP679 pKa = 3.51

Molecular weight:
72.19 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A553KWG5|A0A553KWG5_9ALTE Monovalent cation/H+ antiporter subunit D OS=Aliiglaciecola sp. M165 OX=2593649 GN=FM019_19940 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.2VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.38GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight:
5.11 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4070

0

4070

1360031

17

3453

334.2

37.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.319 ± 0.04

0.971 ± 0.013

5.821 ± 0.035

6.155 ± 0.036

4.48 ± 0.026

6.661 ± 0.039

2.219 ± 0.02

6.4 ± 0.026

5.293 ± 0.038

10.193 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.02

4.609 ± 0.028

3.857 ± 0.024

4.814 ± 0.039

4.42 ± 0.027

6.852 ± 0.034

5.241 ± 0.03

6.945 ± 0.031

1.257 ± 0.016

3.007 ± 0.021

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski