Dinoponera quadriceps (South American ant)
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17673 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6P3YH17|A0A6P3YH17_DINQU homer protein homolog 2-like OS=Dinoponera quadriceps OX=609295 GN=LOC106752136 PE=4 SV=1
MM1 pKa = 7.11 VVLSNFLTPQEE12 pKa = 4.6 GIRR15 pKa = 11.84 HH16 pKa = 5.62 EE17 pKa = 4.32 EE18 pKa = 3.96 QNTTVYY24 pKa = 10.76 INDD27 pKa = 3.7 RR28 pKa = 11.84 EE29 pKa = 4.35 VGKK32 pKa = 8.78 GTFYY36 pKa = 9.91 ITEE39 pKa = 4.36 SVLSWVNSDD48 pKa = 3.51 TQQGFSLEE56 pKa = 4.16 YY57 pKa = 10.02 PHH59 pKa = 7.27 ISLHH63 pKa = 6.32 AISRR67 pKa = 11.84 DD68 pKa = 3.7 EE69 pKa = 3.97 QVHH72 pKa = 5.92 PRR74 pKa = 11.84 QCLYY78 pKa = 11.52 VMVDD82 pKa = 3.56 GKK84 pKa = 11.37 VDD86 pKa = 4.07 LPDD89 pKa = 3.53 MPLPLSPDD97 pKa = 3.18 NGSEE101 pKa = 4.29 NEE103 pKa = 4.72 DD104 pKa = 4.13 DD105 pKa = 6.19 DD106 pKa = 6.56 GDD108 pKa = 4.1 TPITEE113 pKa = 4.07 MRR115 pKa = 11.84 FAPDD119 pKa = 3.05 NTNNLEE125 pKa = 4.9 LMFQAMNACQALHH138 pKa = 7.51 PDD140 pKa = 3.96 PQDD143 pKa = 3.33 SFSDD147 pKa = 3.31 EE148 pKa = 5.23 DD149 pKa = 3.62 IYY151 pKa = 11.56 EE152 pKa = 4.56 DD153 pKa = 3.43 AAEE156 pKa = 4.96 DD157 pKa = 3.49 YY158 pKa = 11.34 GEE160 pKa = 4.04 YY161 pKa = 10.26 SEE163 pKa = 5.55 EE164 pKa = 4.17 VGAGDD169 pKa = 4.62 APYY172 pKa = 10.4 ILPTEE177 pKa = 4.13 QMGASHH183 pKa = 6.78 NGSEE187 pKa = 4.58 AEE189 pKa = 3.84 EE190 pKa = 4.32 AMDD193 pKa = 3.75 VEE195 pKa = 4.86 AGQFEE200 pKa = 4.57 DD201 pKa = 5.56 AEE203 pKa = 4.32 EE204 pKa = 4.4 DD205 pKa = 3.54 PP206 pKa = 5.23
Molecular weight: 22.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.503
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.592
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 3.986
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.821
Patrickios 0.846
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A6P3XQ50|A0A6P3XQ50_DINQU zinc finger protein 346-like OS=Dinoponera quadriceps OX=609295 GN=LOC106747538 PE=4 SV=1
MM1 pKa = 7.73 RR2 pKa = 11.84 KK3 pKa = 9.89 CGVEE7 pKa = 3.87 KK8 pKa = 9.09 PTDD11 pKa = 3.51 VTIEE15 pKa = 4.04 CSSRR19 pKa = 11.84 PRR21 pKa = 11.84 VNHH24 pKa = 6.49 FDD26 pKa = 3.21 RR27 pKa = 11.84 TALVQRR33 pKa = 11.84 CDD35 pKa = 3.68 FRR37 pKa = 11.84 SHH39 pKa = 5.71 VLSTSRR45 pKa = 11.84 DD46 pKa = 3.71 PIRR49 pKa = 11.84 NSSNRR54 pKa = 11.84 QLRR57 pKa = 11.84 SRR59 pKa = 11.84 NGEE62 pKa = 3.76 ITSSGDD68 pKa = 3.0 HH69 pKa = 6.24 TNPLFSVSDD78 pKa = 3.45 AGQRR82 pKa = 11.84 PTWSLPLSRR91 pKa = 11.84 YY92 pKa = 8.92 GSPFPRR98 pKa = 11.84 GPSSSLDD105 pKa = 3.44 PLLPFPPGEE114 pKa = 4.25 SARR117 pKa = 11.84 SRR119 pKa = 11.84 IFIPITEE126 pKa = 4.46 PDD128 pKa = 3.19 SRR130 pKa = 11.84 SCFRR134 pKa = 11.84 CCRR137 pKa = 11.84 RR138 pKa = 11.84 SPPEE142 pKa = 3.74 RR143 pKa = 11.84 LVLCGSTALFLNHH156 pKa = 6.54 SPTCAVCHH164 pKa = 6.26 DD165 pKa = 4.13 VGPPRR170 pKa = 11.84 LLLSLSRR177 pKa = 11.84 FKK179 pKa = 10.82 PSTVIRR185 pKa = 11.84 DD186 pKa = 3.83 DD187 pKa = 4.65 DD188 pKa = 4.04 NGASSGQEE196 pKa = 2.84 RR197 pKa = 11.84 RR198 pKa = 11.84 TEE200 pKa = 3.82 MEE202 pKa = 3.74 RR203 pKa = 11.84 KK204 pKa = 9.33 VKK206 pKa = 10.21 IEE208 pKa = 3.97 RR209 pKa = 11.84 EE210 pKa = 4.01 VHH212 pKa = 5.41 HH213 pKa = 6.48 VGKK216 pKa = 10.41 IAGTKK221 pKa = 9.1 ARR223 pKa = 11.84 YY224 pKa = 8.92 VLAVSVNKK232 pKa = 10.54 LKK234 pKa = 10.89 RR235 pKa = 11.84 HH236 pKa = 5.48 FAYY239 pKa = 10.33 ASAQHH244 pKa = 6.15 KK245 pKa = 9.59 RR246 pKa = 11.84 RR247 pKa = 11.84 KK248 pKa = 7.73 TRR250 pKa = 11.84 ALTHH254 pKa = 6.32 RR255 pKa = 11.84 PARR258 pKa = 11.84 SYY260 pKa = 9.6 TQLNARR266 pKa = 11.84 TYY268 pKa = 8.6 AHH270 pKa = 6.5 TRR272 pKa = 11.84 NKK274 pKa = 10.05 HH275 pKa = 5.15 AGATIDD281 pKa = 3.54 FTVCLRR287 pKa = 11.84 SLSLSLSLFSFLFEE301 pKa = 4.04 EE302 pKa = 4.64 LHH304 pKa = 5.8 AHH306 pKa = 6.48 GARR309 pKa = 11.84 FF310 pKa = 3.27
Molecular weight: 34.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.516
IPC_protein 10.379
Toseland 10.687
ProMoST 10.438
Dawson 10.76
Bjellqvist 10.526
Wikipedia 10.994
Rodwell 10.877
Grimsley 10.804
Solomon 10.921
Lehninger 10.891
Nozaki 10.716
DTASelect 10.496
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.716
Patrickios 10.57
IPC_peptide 10.935
IPC2_peptide 9.867
IPC2.peptide.svr19 8.657
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11037
6636
17673
12470770
9
16744
705.6
79.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.824 ± 0.025
1.863 ± 0.019
5.625 ± 0.016
6.974 ± 0.032
3.249 ± 0.015
5.659 ± 0.021
2.519 ± 0.01
5.133 ± 0.015
6.038 ± 0.028
8.88 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.29 ± 0.009
4.646 ± 0.015
5.324 ± 0.024
4.492 ± 0.02
6.018 ± 0.02
8.358 ± 0.023
6.0 ± 0.015
6.172 ± 0.014
1.025 ± 0.007
2.911 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here