Arthrobacter phage Tweety19

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9W266|A0A7G9W266_9CAUD HNH endonuclease OS=Arthrobacter phage Tweety19 OX=2768133 GN=69 PE=4 SV=1
MM1 pKa = 7.87DD2 pKa = 5.4FEE4 pKa = 6.29LEE6 pKa = 4.6DD7 pKa = 3.56VDD9 pKa = 5.86LEE11 pKa = 4.48LNEE14 pKa = 4.79RR15 pKa = 11.84EE16 pKa = 4.59YY17 pKa = 10.62ICPACRR23 pKa = 11.84LTHH26 pKa = 5.52WQGAPDD32 pKa = 3.92PCDD35 pKa = 3.34RR36 pKa = 11.84SS37 pKa = 3.51

Molecular weight: 4.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9W259|A0A7G9W259_9CAUD Uncharacterized protein OS=Arthrobacter phage Tweety19 OX=2768133 GN=63 PE=4 SV=1
MM1 pKa = 7.48SRR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 9.82LKK7 pKa = 10.69PNLPADD13 pKa = 3.45WHH15 pKa = 6.0EE16 pKa = 4.52HH17 pKa = 6.55DD18 pKa = 4.07SAEE21 pKa = 4.27ANGRR25 pKa = 11.84HH26 pKa = 5.2ITPGTEE32 pKa = 3.46VSIRR36 pKa = 11.84GEE38 pKa = 3.61RR39 pKa = 11.84GRR41 pKa = 11.84FRR43 pKa = 11.84FIKK46 pKa = 10.26RR47 pKa = 11.84VTRR50 pKa = 11.84DD51 pKa = 3.3SGVEE55 pKa = 3.7WLDD58 pKa = 3.26FWGGPKK64 pKa = 10.25GCEE67 pKa = 3.43NWRR70 pKa = 11.84SFRR73 pKa = 11.84PEE75 pKa = 3.26QVRR78 pKa = 11.84RR79 pKa = 11.84VHH81 pKa = 6.69RR82 pKa = 11.84VNKK85 pKa = 8.56TDD87 pKa = 3.24KK88 pKa = 11.04ALAAAHH94 pKa = 6.47KK95 pKa = 9.89AKK97 pKa = 10.7KK98 pKa = 9.15EE99 pKa = 3.9ALRR102 pKa = 11.84SS103 pKa = 3.62

Molecular weight: 11.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12599

37

843

185.3

20.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.311 ± 0.442

0.595 ± 0.095

6.104 ± 0.358

6.628 ± 0.41

3.27 ± 0.197

8.294 ± 0.388

1.722 ± 0.161

4.342 ± 0.292

3.953 ± 0.26

8.89 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.929 ± 0.14

2.722 ± 0.247

5.143 ± 0.217

2.413 ± 0.171

7.524 ± 0.426

5.445 ± 0.231

6.024 ± 0.279

7.604 ± 0.332

1.579 ± 0.153

2.508 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski