Clostridium sp. CAG:594

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6D6S1|R6D6S1_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:594 OX=1262826 GN=BN726_01042 PE=4 SV=1
MM1 pKa = 7.19TALEE5 pKa = 4.75LIKK8 pKa = 10.92KK9 pKa = 10.18NMINSGLDD17 pKa = 3.07SSEE20 pKa = 4.15TVGLFSTYY28 pKa = 9.32QVSDD32 pKa = 4.31LDD34 pKa = 4.28CDD36 pKa = 4.37CVNGDD41 pKa = 4.38CSTDD45 pKa = 3.4GYY47 pKa = 11.41EE48 pKa = 4.04SS49 pKa = 3.38

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6CVN0|R6CVN0_9CLOT Lyzozyme M1 (1 4-beta-N-acetylmuramidase) OS=Clostridium sp. CAG:594 OX=1262826 GN=BN726_00027 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 10.42RR3 pKa = 11.84PFQPNNHH10 pKa = 5.68KK11 pKa = 10.29RR12 pKa = 11.84KK13 pKa = 9.25KK14 pKa = 8.96NHH16 pKa = 5.43GFFARR21 pKa = 11.84KK22 pKa = 8.7LAGTNVINKK31 pKa = 8.92RR32 pKa = 11.84RR33 pKa = 11.84AKK35 pKa = 10.04GRR37 pKa = 11.84KK38 pKa = 8.76KK39 pKa = 10.63LSAA42 pKa = 3.95

Molecular weight: 4.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1188

0

1188

338558

29

1531

285.0

32.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.509 ± 0.061

1.099 ± 0.026

6.415 ± 0.061

6.952 ± 0.08

4.363 ± 0.06

5.197 ± 0.063

1.188 ± 0.027

9.472 ± 0.085

11.054 ± 0.077

9.165 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.542 ± 0.036

7.359 ± 0.082

2.369 ± 0.036

1.749 ± 0.035

3.023 ± 0.043

6.425 ± 0.064

5.21 ± 0.064

6.334 ± 0.06

0.526 ± 0.019

5.037 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski