Amino acid dipepetide frequency for Clostridium sp. CAG:594

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	2.369AlaAla: 2.369 ± 0.104	0.567AlaCys: 0.567 ± 0.048	2.065AlaAsp: 2.065 ± 0.089	2.233AlaGlu: 2.233 ± 0.086	2.082AlaPhe: 2.082 ± 0.096	2.517AlaGly: 2.517 ± 0.096	0.626AlaHis: 0.626 ± 0.052	4.247AlaIle: 4.247 ± 0.122	4.809AlaLys: 4.809 ± 0.124	4.927AlaLeu: 4.927 ± 0.118	1.099AlaMet: 1.099 ± 0.057	2.644AlaAsn: 2.644 ± 0.111	1.054AlaPro: 1.054 ± 0.057	0.969AlaGln: 0.969 ± 0.066	1.743AlaArg: 1.743 ± 0.094	3.778AlaSer: 3.778 ± 0.114	2.295AlaThr: 2.295 ± 0.087	2.717AlaVal: 2.717 ± 0.103	0.213AlaTrp: 0.213 ± 0.026	2.136AlaTyr: 2.136 ± 0.076	0.0AlaXaa: 0.0 ± 0.0
Cys	0.591CysAla: 0.591 ± 0.048	0.18CysCys: 0.18 ± 0.025	0.842CysAsp: 0.842 ± 0.048	0.647CysGlu: 0.647 ± 0.044	0.623CysPhe: 0.623 ± 0.042	0.803CysGly: 0.803 ± 0.056	0.189CysHis: 0.189 ± 0.023	0.718CysIle: 0.718 ± 0.049	1.134CysLys: 1.134 ± 0.067	0.942CysLeu: 0.942 ± 0.048	0.198CysMet: 0.198 ± 0.022	0.673CysAsn: 0.673 ± 0.051	0.408CysPro: 0.408 ± 0.05	0.204CysGln: 0.204 ± 0.024	0.213CysArg: 0.213 ± 0.027	0.603CysSer: 0.603 ± 0.048	0.608CysThr: 0.608 ± 0.043	0.718CysVal: 0.718 ± 0.047	0.071CysTrp: 0.071 ± 0.013	0.629CysTyr: 0.629 ± 0.042	0.0CysXaa: 0.0 ± 0.0
Asp	3.081AspAla: 3.081 ± 0.098	0.6AspCys: 0.6 ± 0.048	4.519AspAsp: 4.519 ± 0.151	5.423AspGlu: 5.423 ± 0.15	2.895AspPhe: 2.895 ± 0.091	3.352AspGly: 3.352 ± 0.107	0.585AspHis: 0.585 ± 0.039	6.052AspIle: 6.052 ± 0.15	7.34AspLys: 7.34 ± 0.155	5.609AspLeu: 5.609 ± 0.147	1.887AspMet: 1.887 ± 0.094	4.534AspAsn: 4.534 ± 0.143	1.205AspPro: 1.205 ± 0.08	0.7AspGln: 0.7 ± 0.051	1.521AspArg: 1.521 ± 0.061	3.462AspSer: 3.462 ± 0.105	2.98AspThr: 2.98 ± 0.093	4.51AspVal: 4.51 ± 0.115	0.301AspTrp: 0.301 ± 0.036	3.586AspTyr: 3.586 ± 0.098	0.006AspXaa: 0.006 ± 0.004
Glu	3.282GluAla: 3.282 ± 0.121	0.588GluCys: 0.588 ± 0.043	4.307GluAsp: 4.307 ± 0.12	6.684GluGlu: 6.684 ± 0.19	2.709GluPhe: 2.709 ± 0.099	2.679GluGly: 2.679 ± 0.09	0.744GluHis: 0.744 ± 0.045	6.649GluIle: 6.649 ± 0.159	8.796GluLys: 8.796 ± 0.199	6.486GluLeu: 6.486 ± 0.167	1.828GluMet: 1.828 ± 0.077	5.402GluAsn: 5.402 ± 0.146	1.229GluPro: 1.229 ± 0.058	1.382GluGln: 1.382 ± 0.077	2.342GluArg: 2.342 ± 0.09	3.385GluSer: 3.385 ± 0.111	3.054GluThr: 3.054 ± 0.106	4.398GluVal: 4.398 ± 0.146	0.289GluTrp: 0.289 ± 0.038	3.287GluTyr: 3.287 ± 0.116	0.003GluXaa: 0.003 ± 0.003
Phe	1.831PheAla: 1.831 ± 0.073	0.455PheCys: 0.455 ± 0.037	3.364PheAsp: 3.364 ± 0.109	2.623PheGlu: 2.623 ± 0.094	1.811PhePhe: 1.811 ± 0.075	2.443PheGly: 2.443 ± 0.101	0.425PheHis: 0.425 ± 0.032	4.649PheIle: 4.649 ± 0.166	4.304PheLys: 4.304 ± 0.134	4.1PheLeu: 4.1 ± 0.145	1.181PheMet: 1.181 ± 0.055	3.521PheAsn: 3.521 ± 0.123	1.17PhePro: 1.17 ± 0.064	0.656PheGln: 0.656 ± 0.049	1.078PheArg: 1.078 ± 0.059	2.966PheSer: 2.966 ± 0.105	2.186PheThr: 2.186 ± 0.077	2.567PheVal: 2.567 ± 0.083	0.266PheTrp: 0.266 ± 0.025	2.038PheTyr: 2.038 ± 0.075	0.0PheXaa: 0.0 ± 0.0
Gly	2.806GlyAla: 2.806 ± 0.115	0.868GlyCys: 0.868 ± 0.056	2.957GlyAsp: 2.957 ± 0.107	2.971GlyGlu: 2.971 ± 0.12	2.647GlyPhe: 2.647 ± 0.099	2.75GlyGly: 2.75 ± 0.127	0.842GlyHis: 0.842 ± 0.05	5.157GlyIle: 5.157 ± 0.118	5.373GlyLys: 5.373 ± 0.127	4.327GlyLeu: 4.327 ± 0.148	1.3GlyMet: 1.3 ± 0.069	3.09GlyAsn: 3.09 ± 0.114	0.877GlyPro: 0.877 ± 0.062	0.963GlyGln: 0.963 ± 0.055	1.722GlyArg: 1.722 ± 0.083	3.14GlySer: 3.14 ± 0.103	3.048GlyThr: 3.048 ± 0.103	3.621GlyVal: 3.621 ± 0.102	0.281GlyTrp: 0.281 ± 0.029	3.225GlyTyr: 3.225 ± 0.102	0.003GlyXaa: 0.003 ± 0.003
His	0.523HisAla: 0.523 ± 0.039	0.142HisCys: 0.142 ± 0.021	0.697HisAsp: 0.697 ± 0.047	0.771HisGlu: 0.771 ± 0.047	0.644HisPhe: 0.644 ± 0.047	0.747HisGly: 0.747 ± 0.057	0.224HisHis: 0.224 ± 0.029	1.031HisIle: 1.031 ± 0.055	1.004HisLys: 1.004 ± 0.057	1.054HisLeu: 1.054 ± 0.064	0.346HisMet: 0.346 ± 0.032	0.942HisAsn: 0.942 ± 0.061	0.502HisPro: 0.502 ± 0.043	0.251HisGln: 0.251 ± 0.028	0.446HisArg: 0.446 ± 0.037	0.656HisSer: 0.656 ± 0.047	0.647HisThr: 0.647 ± 0.044	0.659HisVal: 0.659 ± 0.047	0.035HisTrp: 0.035 ± 0.012	0.555HisTyr: 0.555 ± 0.04	0.0HisXaa: 0.0 ± 0.0
Ile	4.023IleAla: 4.023 ± 0.122	1.152IleCys: 1.152 ± 0.065	6.253IleAsp: 6.253 ± 0.124	5.869IleGlu: 5.869 ± 0.139	3.846IlePhe: 3.846 ± 0.149	4.688IleGly: 4.688 ± 0.134	0.88IleHis: 0.88 ± 0.055	9.975IleIle: 9.975 ± 0.27	10.825IleLys: 10.825 ± 0.205	8.586IleLeu: 8.586 ± 0.22	2.106IleMet: 2.106 ± 0.079	7.594IleAsn: 7.594 ± 0.174	2.626IlePro: 2.626 ± 0.097	1.096IleGln: 1.096 ± 0.055	2.717IleArg: 2.717 ± 0.098	6.711IleSer: 6.711 ± 0.158	5.52IleThr: 5.52 ± 0.132	5.757IleVal: 5.757 ± 0.129	0.402IleTrp: 0.402 ± 0.04	4.088IleTyr: 4.088 ± 0.138	0.009IleXaa: 0.009 ± 0.005
Lys	4.401LysAla: 4.401 ± 0.123	0.892LysCys: 0.892 ± 0.059	8.055LysAsp: 8.055 ± 0.164	11.165LysGlu: 11.165 ± 0.218	3.223LysPhe: 3.223 ± 0.101	4.699LysGly: 4.699 ± 0.105	1.158LysHis: 1.158 ± 0.057	10.119LysIle: 10.119 ± 0.201	13.112LysLys: 13.112 ± 0.245	9.035LysLeu: 9.035 ± 0.188	3.296LysMet: 3.296 ± 0.098	8.492LysAsn: 8.492 ± 0.168	2.136LysPro: 2.136 ± 0.082	2.265LysGln: 2.265 ± 0.086	3.769LysArg: 3.769 ± 0.099	5.399LysSer: 5.399 ± 0.122	6.026LysThr: 6.026 ± 0.155	6.847LysVal: 6.847 ± 0.162	0.582LysTrp: 0.582 ± 0.038	5.869LysTyr: 5.869 ± 0.151	0.003LysXaa: 0.003 ± 0.003
Leu	4.141LeuAla: 4.141 ± 0.117	0.972LeuCys: 0.972 ± 0.054	5.777LeuAsp: 5.777 ± 0.141	6.061LeuGlu: 6.061 ± 0.156	4.496LeuPhe: 4.496 ± 0.149	4.874LeuGly: 4.874 ± 0.135	0.892LeuHis: 0.892 ± 0.052	8.687LeuIle: 8.687 ± 0.236	10.161LeuLys: 10.161 ± 0.207	8.312LeuLeu: 8.312 ± 0.206	2.079LeuMet: 2.079 ± 0.078	6.672LeuAsn: 6.672 ± 0.155	2.298LeuPro: 2.298 ± 0.086	1.436LeuGln: 1.436 ± 0.069	2.726LeuArg: 2.726 ± 0.097	7.39LeuSer: 7.39 ± 0.147	5.042LeuThr: 5.042 ± 0.122	5.373LeuVal: 5.373 ± 0.14	0.473LeuTrp: 0.473 ± 0.041	3.781LeuTyr: 3.781 ± 0.127	0.006LeuXaa: 0.006 ± 0.004
Met	1.306MetAla: 1.306 ± 0.075	0.201MetCys: 0.201 ± 0.024	1.613MetAsp: 1.613 ± 0.075	1.486MetGlu: 1.486 ± 0.07	1.397MetPhe: 1.397 ± 0.107	1.161MetGly: 1.161 ± 0.064	0.402MetHis: 0.402 ± 0.032	2.475MetIle: 2.475 ± 0.08	2.883MetLys: 2.883 ± 0.086	2.522MetLeu: 2.522 ± 0.088	0.662MetMet: 0.662 ± 0.047	1.722MetAsn: 1.722 ± 0.067	0.945MetPro: 0.945 ± 0.05	0.673MetGln: 0.673 ± 0.049	0.735MetArg: 0.735 ± 0.05	1.311MetSer: 1.311 ± 0.058	1.217MetThr: 1.217 ± 0.07	1.548MetVal: 1.548 ± 0.079	0.124MetTrp: 0.124 ± 0.021	1.04MetTyr: 1.04 ± 0.06	0.0MetXaa: 0.0 ± 0.0
Asn	3.078AsnAla: 3.078 ± 0.112	0.665AsnCys: 0.665 ± 0.042	4.617AsnAsp: 4.617 ± 0.136	5.131AsnGlu: 5.131 ± 0.135	2.924AsnPhe: 2.924 ± 0.086	4.029AsnGly: 4.029 ± 0.13	0.868AsnHis: 0.868 ± 0.049	7.16AsnIle: 7.16 ± 0.157	8.643AsnLys: 8.643 ± 0.161	6.531AsnLeu: 6.531 ± 0.166	2.041AsnMet: 2.041 ± 0.089	6.029AsnAsn: 6.029 ± 0.192	2.026AsnPro: 2.026 ± 0.083	1.22AsnGln: 1.22 ± 0.066	1.725AsnArg: 1.725 ± 0.067	4.026AsnSer: 4.026 ± 0.125	3.565AsnThr: 3.565 ± 0.13	4.723AsnVal: 4.723 ± 0.144	0.369AsnTrp: 0.369 ± 0.037	4.218AsnTyr: 4.218 ± 0.12	0.003AsnXaa: 0.003 ± 0.003
Pro	1.069ProAla: 1.069 ± 0.057	0.281ProCys: 0.281 ± 0.032	1.362ProAsp: 1.362 ± 0.066	1.69ProGlu: 1.69 ± 0.079	1.238ProPhe: 1.238 ± 0.056	1.276ProGly: 1.276 ± 0.075	0.34ProHis: 0.34 ± 0.032	2.032ProIle: 2.032 ± 0.095	2.248ProLys: 2.248 ± 0.081	2.085ProLeu: 2.085 ± 0.076	0.543ProMet: 0.543 ± 0.046	1.701ProAsn: 1.701 ± 0.074	0.325ProPro: 0.325 ± 0.034	0.414ProGln: 0.414 ± 0.035	0.673ProArg: 0.673 ± 0.051	1.784ProSer: 1.784 ± 0.07	1.311ProThr: 1.311 ± 0.071	1.769ProVal: 1.769 ± 0.074	0.213ProTrp: 0.213 ± 0.029	1.338ProTyr: 1.338 ± 0.066	0.003ProXaa: 0.003 ± 0.003
Gln	0.987GlnAla: 0.987 ± 0.053	0.089GlnCys: 0.089 ± 0.014	1.049GlnAsp: 1.049 ± 0.064	1.102GlnGlu: 1.102 ± 0.056	0.647GlnPhe: 0.647 ± 0.047	0.981GlnGly: 0.981 ± 0.047	0.174GlnHis: 0.174 ± 0.023	1.778GlnIle: 1.778 ± 0.075	2.035GlnLys: 2.035 ± 0.088	1.323GlnLeu: 1.323 ± 0.071	0.614GlnMet: 0.614 ± 0.042	1.512GlnAsn: 1.512 ± 0.066	0.322GlnPro: 0.322 ± 0.03	0.366GlnGln: 0.366 ± 0.032	0.753GlnArg: 0.753 ± 0.048	0.969GlnSer: 0.969 ± 0.059	0.987GlnThr: 0.987 ± 0.055	1.057GlnVal: 1.057 ± 0.059	0.086GlnTrp: 0.086 ± 0.016	0.662GlnTyr: 0.662 ± 0.049	0.0GlnXaa: 0.0 ± 0.0
Arg	1.323ArgAla: 1.323 ± 0.068	0.411ArgCys: 0.411 ± 0.037	1.622ArgAsp: 1.622 ± 0.071	2.121ArgGlu: 2.121 ± 0.098	1.264ArgPhe: 1.264 ± 0.063	1.542ArgGly: 1.542 ± 0.074	0.47ArgHis: 0.47 ± 0.04	2.635ArgIle: 2.635 ± 0.094	3.406ArgLys: 3.406 ± 0.108	2.815ArgLeu: 2.815 ± 0.089	0.957ArgMet: 0.957 ± 0.058	2.118ArgAsn: 2.118 ± 0.082	0.774ArgPro: 0.774 ± 0.056	0.78ArgGln: 0.78 ± 0.046	1.353ArgArg: 1.353 ± 0.065	1.527ArgSer: 1.527 ± 0.079	1.394ArgThr: 1.394 ± 0.068	2.003ArgVal: 2.003 ± 0.093	0.201ArgTrp: 0.201 ± 0.027	1.512ArgTyr: 1.512 ± 0.067	0.0ArgXaa: 0.0 ± 0.0
Ser	2.573SerAla: 2.573 ± 0.082	0.798SerCys: 0.798 ± 0.053	4.18SerAsp: 4.18 ± 0.137	3.447SerGlu: 3.447 ± 0.105	3.618SerPhe: 3.618 ± 0.117	3.751SerGly: 3.751 ± 0.118	0.827SerHis: 0.827 ± 0.051	5.396SerIle: 5.396 ± 0.175	6.977SerLys: 6.977 ± 0.157	6.232SerLeu: 6.232 ± 0.147	1.53SerMet: 1.53 ± 0.068	4.785SerAsn: 4.785 ± 0.149	1.279SerPro: 1.279 ± 0.062	1.066SerGln: 1.066 ± 0.061	1.793SerArg: 1.793 ± 0.072	4.868SerSer: 4.868 ± 0.146	2.815SerThr: 2.815 ± 0.109	4.1SerVal: 4.1 ± 0.116	0.422SerTrp: 0.422 ± 0.035	3.787SerTyr: 3.787 ± 0.125	0.003SerXaa: 0.003 ± 0.003
Thr	2.156ThrAla: 2.156 ± 0.082	0.673ThrCys: 0.673 ± 0.046	3.178ThrAsp: 3.178 ± 0.121	2.785ThrGlu: 2.785 ± 0.097	2.41ThrPhe: 2.41 ± 0.101	3.243ThrGly: 3.243 ± 0.114	0.771ThrHis: 0.771 ± 0.051	4.906ThrIle: 4.906 ± 0.123	5.296ThrLys: 5.296 ± 0.109	5.03ThrLeu: 5.03 ± 0.138	1.022ThrMet: 1.022 ± 0.056	3.666ThrAsn: 3.666 ± 0.132	1.56ThrPro: 1.56 ± 0.07	0.845ThrGln: 0.845 ± 0.057	1.412ThrArg: 1.412 ± 0.065	3.917ThrSer: 3.917 ± 0.136	3.09ThrThr: 3.09 ± 0.148	3.048ThrVal: 3.048 ± 0.104	0.266ThrTrp: 0.266 ± 0.028	2.827ThrTyr: 2.827 ± 0.107	0.0ThrXaa: 0.0 ± 0.0
Val	2.921ValAla: 2.921 ± 0.098	0.857ValCys: 0.857 ± 0.048	3.491ValAsp: 3.491 ± 0.107	3.435ValGlu: 3.435 ± 0.117	2.638ValPhe: 2.638 ± 0.09	3.542ValGly: 3.542 ± 0.111	0.715ValHis: 0.715 ± 0.054	6.602ValIle: 6.602 ± 0.147	6.498ValLys: 6.498 ± 0.14	6.342ValLeu: 6.342 ± 0.165	1.554ValMet: 1.554 ± 0.078	4.126ValAsn: 4.126 ± 0.114	1.692ValPro: 1.692 ± 0.075	1.025ValGln: 1.025 ± 0.06	1.941ValArg: 1.941 ± 0.081	5.03ValSer: 5.03 ± 0.141	3.657ValThr: 3.657 ± 0.122	4.188ValVal: 4.188 ± 0.122	0.375ValTrp: 0.375 ± 0.042	2.709ValTyr: 2.709 ± 0.096	0.006ValXaa: 0.006 ± 0.004
Trp	0.251TrpAla: 0.251 ± 0.027	0.106TrpCys: 0.106 ± 0.017	0.349TrpAsp: 0.349 ± 0.037	0.275TrpGlu: 0.275 ± 0.033	0.263TrpPhe: 0.263 ± 0.027	0.242TrpGly: 0.242 ± 0.026	0.077TrpHis: 0.077 ± 0.015	0.425TrpIle: 0.425 ± 0.04	0.372TrpLys: 0.372 ± 0.036	0.541TrpLeu: 0.541 ± 0.043	0.136TrpMet: 0.136 ± 0.022	0.467TrpAsn: 0.467 ± 0.042	0.103TrpPro: 0.103 ± 0.014	0.139TrpGln: 0.139 ± 0.021	0.154TrpArg: 0.154 ± 0.023	0.331TrpSer: 0.331 ± 0.035	0.289TrpThr: 0.289 ± 0.033	0.287TrpVal: 0.287 ± 0.029	0.083TrpTrp: 0.083 ± 0.029	0.366TrpTyr: 0.366 ± 0.038	0.006TrpXaa: 0.006 ± 0.004
Tyr	2.378TyrAla: 2.378 ± 0.088	0.499TyrCys: 0.499 ± 0.04	3.849TyrAsp: 3.849 ± 0.115	3.606TyrGlu: 3.606 ± 0.109	2.251TyrPhe: 2.251 ± 0.093	2.647TyrGly: 2.647 ± 0.082	0.668TyrHis: 0.668 ± 0.042	4.026TyrIle: 4.026 ± 0.123	5.314TyrLys: 5.314 ± 0.155	4.85TyrLeu: 4.85 ± 0.137	1.037TyrMet: 1.037 ± 0.054	3.893TyrAsn: 3.893 ± 0.119	1.164TyrPro: 1.164 ± 0.058	1.04TyrGln: 1.04 ± 0.057	1.406TyrArg: 1.406 ± 0.06	2.992TyrSer: 2.992 ± 0.102	2.369TyrThr: 2.369 ± 0.1	3.453TyrVal: 3.453 ± 0.114	0.21TyrTrp: 0.21 ± 0.027	2.72TyrTyr: 2.72 ± 0.124	0.003TyrXaa: 0.003 ± 0.003
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.006XaaAsp: 0.006 ± 0.005	0.0XaaGlu: 0.0 ± 0.0	0.009XaaPhe: 0.009 ± 0.006	0.003XaaGly: 0.003 ± 0.003	0.0XaaHis: 0.0 ± 0.0	0.003XaaIle: 0.003 ± 0.003	0.015XaaLys: 0.015 ± 0.007	0.0XaaLeu: 0.0 ± 0.0	0.003XaaMet: 0.003 ± 0.003	0.006XaaAsn: 0.006 ± 0.004	0.0XaaPro: 0.0 ± 0.0	0.003XaaGln: 0.003 ± 0.003	0.003XaaArg: 0.003 ± 0.003	0.003XaaSer: 0.003 ± 0.003	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.056XaaXaa: 0.056 ± 0.019

Statistics based on 1188 proteins (338558 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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