Amino acid dipepetide frequency for Nitrospira moscoviensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.445AlaAla: 13.445 ± 0.12	1.15AlaCys: 1.15 ± 0.032	5.417AlaAsp: 5.417 ± 0.068	6.413AlaGlu: 6.413 ± 0.083	3.434AlaPhe: 3.434 ± 0.049	9.309AlaGly: 9.309 ± 0.101	2.243AlaHis: 2.243 ± 0.044	5.011AlaIle: 5.011 ± 0.061	4.188AlaLys: 4.188 ± 0.068	11.533AlaLeu: 11.533 ± 0.13	2.886AlaMet: 2.886 ± 0.053	2.177AlaAsn: 2.177 ± 0.042	4.819AlaPro: 4.819 ± 0.079	4.084AlaGln: 4.084 ± 0.063	7.251AlaArg: 7.251 ± 0.077	5.48AlaSer: 5.48 ± 0.076	4.977AlaThr: 4.977 ± 0.064	8.747AlaVal: 8.747 ± 0.093	1.439AlaTrp: 1.439 ± 0.037	2.533AlaTyr: 2.533 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	1.065CysAla: 1.065 ± 0.03	0.176CysCys: 0.176 ± 0.01	0.507CysAsp: 0.507 ± 0.02	0.511CysGlu: 0.511 ± 0.021	0.33CysPhe: 0.33 ± 0.013	0.959CysGly: 0.959 ± 0.03	0.347CysHis: 0.347 ± 0.018	0.35CysIle: 0.35 ± 0.015	0.272CysLys: 0.272 ± 0.014	1.111CysLeu: 1.111 ± 0.031	0.195CysMet: 0.195 ± 0.012	0.202CysAsn: 0.202 ± 0.013	0.629CysPro: 0.629 ± 0.024	0.302CysGln: 0.302 ± 0.013	0.928CysArg: 0.928 ± 0.03	0.597CysSer: 0.597 ± 0.024	0.487CysThr: 0.487 ± 0.019	0.732CysVal: 0.732 ± 0.023	0.131CysTrp: 0.131 ± 0.009	0.269CysTyr: 0.269 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	5.182AspAla: 5.182 ± 0.072	0.555AspCys: 0.555 ± 0.02	2.608AspAsp: 2.608 ± 0.057	3.333AspGlu: 3.333 ± 0.05	1.732AspPhe: 1.732 ± 0.041	4.365AspGly: 4.365 ± 0.073	1.397AspHis: 1.397 ± 0.03	2.554AspIle: 2.554 ± 0.048	1.671AspLys: 1.671 ± 0.044	5.812AspLeu: 5.812 ± 0.065	1.04AspMet: 1.04 ± 0.027	1.031AspAsn: 1.031 ± 0.028	3.36AspPro: 3.36 ± 0.056	1.972AspGln: 1.972 ± 0.043	4.751AspArg: 4.751 ± 0.065	2.225AspSer: 2.225 ± 0.038	2.292AspThr: 2.292 ± 0.067	4.253AspVal: 4.253 ± 0.062	0.756AspTrp: 0.756 ± 0.024	1.481AspTyr: 1.481 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	6.948GluAla: 6.948 ± 0.085	0.441GluCys: 0.441 ± 0.02	2.735GluAsp: 2.735 ± 0.044	4.067GluGlu: 4.067 ± 0.069	1.941GluPhe: 1.941 ± 0.035	4.29GluGly: 4.29 ± 0.055	1.409GluHis: 1.409 ± 0.031	2.976GluIle: 2.976 ± 0.058	2.385GluLys: 2.385 ± 0.053	6.004GluLeu: 6.004 ± 0.078	1.377GluMet: 1.377 ± 0.031	1.179GluAsn: 1.179 ± 0.032	2.917GluPro: 2.917 ± 0.05	3.041GluGln: 3.041 ± 0.045	5.715GluArg: 5.715 ± 0.066	3.185GluSer: 3.185 ± 0.05	3.16GluThr: 3.16 ± 0.045	4.156GluVal: 4.156 ± 0.07	0.942GluTrp: 0.942 ± 0.025	1.373GluTyr: 1.373 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	3.32PheAla: 3.32 ± 0.053	0.424PheCys: 0.424 ± 0.018	2.125PheAsp: 2.125 ± 0.038	1.983PheGlu: 1.983 ± 0.032	1.412PhePhe: 1.412 ± 0.037	3.022PheGly: 3.022 ± 0.058	0.928PheHis: 0.928 ± 0.031	1.503PheIle: 1.503 ± 0.035	1.123PheLys: 1.123 ± 0.031	3.725PheLeu: 3.725 ± 0.062	0.761PheMet: 0.761 ± 0.024	1.011PheAsn: 1.011 ± 0.031	1.695PhePro: 1.695 ± 0.038	1.219PheGln: 1.219 ± 0.031	2.431PheArg: 2.431 ± 0.04	2.171PheSer: 2.171 ± 0.037	2.118PheThr: 2.118 ± 0.042	2.665PheVal: 2.665 ± 0.045	0.491PheTrp: 0.491 ± 0.02	1.041PheTyr: 1.041 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	7.451GlyAla: 7.451 ± 0.09	0.953GlyCys: 0.953 ± 0.029	3.749GlyAsp: 3.749 ± 0.089	4.468GlyGlu: 4.468 ± 0.057	3.041GlyPhe: 3.041 ± 0.052	6.477GlyGly: 6.477 ± 0.116	1.933GlyHis: 1.933 ± 0.036	4.149GlyIle: 4.149 ± 0.053	3.452GlyLys: 3.452 ± 0.062	8.636GlyLeu: 8.636 ± 0.087	2.037GlyMet: 2.037 ± 0.045	1.937GlyAsn: 1.937 ± 0.078	3.412GlyPro: 3.412 ± 0.056	2.836GlyGln: 2.836 ± 0.047	6.332GlyArg: 6.332 ± 0.078	4.465GlySer: 4.465 ± 0.061	4.955GlyThr: 4.955 ± 0.077	5.799GlyVal: 5.799 ± 0.075	1.319GlyTrp: 1.319 ± 0.033	2.346GlyTyr: 2.346 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.367HisAla: 2.367 ± 0.045	0.328HisCys: 0.328 ± 0.016	1.309HisAsp: 1.309 ± 0.034	1.438HisGlu: 1.438 ± 0.032	0.821HisPhe: 0.821 ± 0.024	1.994HisGly: 1.994 ± 0.042	0.802HisHis: 0.802 ± 0.029	1.047HisIle: 1.047 ± 0.03	0.669HisLys: 0.669 ± 0.023	2.498HisLeu: 2.498 ± 0.047	0.496HisMet: 0.496 ± 0.02	0.52HisAsn: 0.52 ± 0.019	1.695HisPro: 1.695 ± 0.035	0.911HisGln: 0.911 ± 0.029	2.027HisArg: 2.027 ± 0.043	1.126HisSer: 1.126 ± 0.03	1.058HisThr: 1.058 ± 0.027	1.813HisVal: 1.813 ± 0.036	0.357HisTrp: 0.357 ± 0.015	0.671HisTyr: 0.671 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.164IleAla: 5.164 ± 0.061	0.452IleCys: 0.452 ± 0.021	2.837IleAsp: 2.837 ± 0.044	3.416IleGlu: 3.416 ± 0.059	1.441IlePhe: 1.441 ± 0.032	4.5IleGly: 4.5 ± 0.074	1.203IleHis: 1.203 ± 0.031	2.028IleIle: 2.028 ± 0.043	1.899IleLys: 1.899 ± 0.044	4.496IleLeu: 4.496 ± 0.059	0.886IleMet: 0.886 ± 0.023	1.266IleAsn: 1.266 ± 0.034	2.793IlePro: 2.793 ± 0.047	1.685IleGln: 1.685 ± 0.04	3.476IleArg: 3.476 ± 0.047	2.32IleSer: 2.32 ± 0.042	2.796IleThr: 2.796 ± 0.059	4.148IleVal: 4.148 ± 0.061	0.509IleTrp: 0.509 ± 0.02	1.01IleTyr: 1.01 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.04LysAla: 4.04 ± 0.073	0.209LysCys: 0.209 ± 0.011	2.194LysAsp: 2.194 ± 0.047	2.675LysGlu: 2.675 ± 0.061	0.989LysPhe: 0.989 ± 0.035	2.958LysGly: 2.958 ± 0.059	0.832LysHis: 0.832 ± 0.025	1.913LysIle: 1.913 ± 0.048	1.965LysLys: 1.965 ± 0.046	3.434LysLeu: 3.434 ± 0.057	0.888LysMet: 0.888 ± 0.027	0.955LysAsn: 0.955 ± 0.029	2.257LysPro: 2.257 ± 0.049	1.645LysGln: 1.645 ± 0.038	2.722LysArg: 2.722 ± 0.052	1.922LysSer: 1.922 ± 0.042	2.269LysThr: 2.269 ± 0.046	2.621LysVal: 2.621 ± 0.05	0.376LysTrp: 0.376 ± 0.018	0.778LysTyr: 0.778 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	12.098LeuAla: 12.098 ± 0.123	1.135LeuCys: 1.135 ± 0.032	5.694LeuAsp: 5.694 ± 0.069	5.805LeuGlu: 5.805 ± 0.073	3.938LeuPhe: 3.938 ± 0.057	7.681LeuGly: 7.681 ± 0.082	2.467LeuHis: 2.467 ± 0.047	5.074LeuIle: 5.074 ± 0.072	4.195LeuLys: 4.195 ± 0.067	11.192LeuLeu: 11.192 ± 0.13	2.209LeuMet: 2.209 ± 0.037	2.768LeuAsn: 2.768 ± 0.047	5.676LeuPro: 5.676 ± 0.072	3.502LeuGln: 3.502 ± 0.055	7.796LeuArg: 7.796 ± 0.084	6.611LeuSer: 6.611 ± 0.074	6.25LeuThr: 6.25 ± 0.071	7.761LeuVal: 7.761 ± 0.082	1.319LeuTrp: 1.319 ± 0.032	2.636LeuTyr: 2.636 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	2.746MetAla: 2.746 ± 0.042	0.158MetCys: 0.158 ± 0.011	1.169MetAsp: 1.169 ± 0.03	1.318MetGlu: 1.318 ± 0.03	0.631MetPhe: 0.631 ± 0.022	1.709MetGly: 1.709 ± 0.041	0.426MetHis: 0.426 ± 0.016	1.144MetIle: 1.144 ± 0.03	1.282MetLys: 1.282 ± 0.037	2.14MetLeu: 2.14 ± 0.041	0.554MetMet: 0.554 ± 0.021	0.748MetAsn: 0.748 ± 0.022	1.519MetPro: 1.519 ± 0.034	0.838MetGln: 0.838 ± 0.026	1.621MetArg: 1.621 ± 0.044	1.351MetSer: 1.351 ± 0.028	1.604MetThr: 1.604 ± 0.035	1.683MetVal: 1.683 ± 0.037	0.221MetTrp: 0.221 ± 0.013	0.396MetTyr: 0.396 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.187AsnAla: 2.187 ± 0.047	0.246AsnCys: 0.246 ± 0.014	1.2AsnAsp: 1.2 ± 0.064	1.261AsnGlu: 1.261 ± 0.031	0.852AsnPhe: 0.852 ± 0.03	2.17AsnGly: 2.17 ± 0.051	0.593AsnHis: 0.593 ± 0.021	1.147AsnIle: 1.147 ± 0.033	0.785AsnLys: 0.785 ± 0.03	2.618AsnLeu: 2.618 ± 0.048	0.52AsnMet: 0.52 ± 0.019	0.645AsnAsn: 0.645 ± 0.03	1.814AsnPro: 1.814 ± 0.045	0.946AsnGln: 0.946 ± 0.03	1.865AsnArg: 1.865 ± 0.037	1.06AsnSer: 1.06 ± 0.034	1.117AsnThr: 1.117 ± 0.036	1.943AsnVal: 1.943 ± 0.046	0.342AsnTrp: 0.342 ± 0.015	0.626AsnTyr: 0.626 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	6.321ProAla: 6.321 ± 0.089	0.47ProCys: 0.47 ± 0.019	3.535ProAsp: 3.535 ± 0.063	3.542ProGlu: 3.542 ± 0.051	2.006ProPhe: 2.006 ± 0.039	4.29ProGly: 4.29 ± 0.06	1.283ProHis: 1.283 ± 0.031	2.507ProIle: 2.507 ± 0.047	1.812ProLys: 1.812 ± 0.045	5.33ProLeu: 5.33 ± 0.068	1.228ProMet: 1.228 ± 0.028	1.32ProAsn: 1.32 ± 0.036	3.323ProPro: 3.323 ± 0.066	1.732ProGln: 1.732 ± 0.036	3.088ProArg: 3.088 ± 0.061	3.39ProSer: 3.39 ± 0.057	2.706ProThr: 2.706 ± 0.052	4.405ProVal: 4.405 ± 0.064	0.742ProTrp: 0.742 ± 0.024	1.335ProTyr: 1.335 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	4.743GlnAla: 4.743 ± 0.076	0.287GlnCys: 0.287 ± 0.016	1.879GlnAsp: 1.879 ± 0.037	2.436GlnGlu: 2.436 ± 0.045	1.277GlnPhe: 1.277 ± 0.031	2.759GlnGly: 2.759 ± 0.043	0.822GlnHis: 0.822 ± 0.024	1.785GlnIle: 1.785 ± 0.033	1.258GlnLys: 1.258 ± 0.032	3.738GlnLeu: 3.738 ± 0.055	0.807GlnMet: 0.807 ± 0.025	0.734GlnAsn: 0.734 ± 0.026	2.028GlnPro: 2.028 ± 0.041	1.775GlnGln: 1.775 ± 0.036	2.93GlnArg: 2.93 ± 0.056	2.014GlnSer: 2.014 ± 0.042	1.997GlnThr: 1.997 ± 0.038	2.697GlnVal: 2.697 ± 0.044	0.577GlnTrp: 0.577 ± 0.02	0.868GlnTyr: 0.868 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	7.162ArgAla: 7.162 ± 0.087	0.797ArgCys: 0.797 ± 0.027	3.835ArgAsp: 3.835 ± 0.057	4.819ArgGlu: 4.819 ± 0.074	2.998ArgPhe: 2.998 ± 0.05	4.62ArgGly: 4.62 ± 0.063	2.081ArgHis: 2.081 ± 0.043	3.927ArgIle: 3.927 ± 0.051	2.532ArgLys: 2.532 ± 0.047	8.905ArgLeu: 8.905 ± 0.104	2.085ArgMet: 2.085 ± 0.041	1.714ArgAsn: 1.714 ± 0.034	3.825ArgPro: 3.825 ± 0.059	3.129ArgGln: 3.129 ± 0.053	6.734ArgArg: 6.734 ± 0.099	4.046ArgSer: 4.046 ± 0.063	3.929ArgThr: 3.929 ± 0.057	5.314ArgVal: 5.314 ± 0.059	1.263ArgTrp: 1.263 ± 0.037	2.306ArgTyr: 2.306 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	5.384SerAla: 5.384 ± 0.065	0.613SerCys: 0.613 ± 0.02	2.689SerAsp: 2.689 ± 0.042	2.77SerGlu: 2.77 ± 0.045	2.174SerPhe: 2.174 ± 0.036	4.957SerGly: 4.957 ± 0.074	1.317SerHis: 1.317 ± 0.027	2.605SerIle: 2.605 ± 0.051	1.743SerLys: 1.743 ± 0.038	6.109SerLeu: 6.109 ± 0.083	1.402SerMet: 1.402 ± 0.032	1.252SerAsn: 1.252 ± 0.033	3.276SerPro: 3.276 ± 0.043	1.889SerGln: 1.889 ± 0.043	4.021SerArg: 4.021 ± 0.057	3.208SerSer: 3.208 ± 0.063	2.748SerThr: 2.748 ± 0.053	4.119SerVal: 4.119 ± 0.06	0.828SerTrp: 0.828 ± 0.027	1.379SerTyr: 1.379 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	5.696ThrAla: 5.696 ± 0.07	0.489ThrCys: 0.489 ± 0.024	2.74ThrAsp: 2.74 ± 0.047	2.812ThrGlu: 2.812 ± 0.049	2.031ThrPhe: 2.031 ± 0.04	4.974ThrGly: 4.974 ± 0.08	1.162ThrHis: 1.162 ± 0.029	3.076ThrIle: 3.076 ± 0.053	1.962ThrLys: 1.962 ± 0.04	5.727ThrLeu: 5.727 ± 0.079	1.241ThrMet: 1.241 ± 0.031	1.346ThrAsn: 1.346 ± 0.036	3.176ThrPro: 3.176 ± 0.053	1.706ThrGln: 1.706 ± 0.032	3.223ThrArg: 3.223 ± 0.052	2.733ThrSer: 2.733 ± 0.053	2.933ThrThr: 2.933 ± 0.064	5.128ThrVal: 5.128 ± 0.07	0.726ThrTrp: 0.726 ± 0.025	1.33ThrTyr: 1.33 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	7.666ValAla: 7.666 ± 0.082	0.745ValCys: 0.745 ± 0.027	3.846ValAsp: 3.846 ± 0.06	4.839ValGlu: 4.839 ± 0.067	2.582ValPhe: 2.582 ± 0.045	5.707ValGly: 5.707 ± 0.076	1.655ValHis: 1.655 ± 0.036	3.878ValIle: 3.878 ± 0.06	3.032ValLys: 3.032 ± 0.058	8.361ValLeu: 8.361 ± 0.088	1.8ValMet: 1.8 ± 0.039	2.013ValAsn: 2.013 ± 0.047	4.138ValPro: 4.138 ± 0.06	2.693ValGln: 2.693 ± 0.045	5.606ValArg: 5.606 ± 0.068	4.544ValSer: 4.544 ± 0.059	4.883ValThr: 4.883 ± 0.08	6.35ValVal: 6.35 ± 0.083	0.946ValTrp: 0.946 ± 0.025	1.767ValTyr: 1.767 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.211TrpAla: 1.211 ± 0.031	0.167TrpCys: 0.167 ± 0.013	0.728TrpAsp: 0.728 ± 0.023	0.795TrpGlu: 0.795 ± 0.025	0.561TrpPhe: 0.561 ± 0.021	0.89TrpGly: 0.89 ± 0.028	0.354TrpHis: 0.354 ± 0.017	0.74TrpIle: 0.74 ± 0.024	0.618TrpLys: 0.618 ± 0.025	1.658TrpLeu: 1.658 ± 0.038	0.398TrpMet: 0.398 ± 0.017	0.47TrpAsn: 0.47 ± 0.019	0.659TrpPro: 0.659 ± 0.022	0.525TrpGln: 0.525 ± 0.018	1.052TrpArg: 1.052 ± 0.031	0.846TrpSer: 0.846 ± 0.023	0.765TrpThr: 0.765 ± 0.026	0.878TrpVal: 0.878 ± 0.024	0.281TrpTrp: 0.281 ± 0.015	0.398TrpTyr: 0.398 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.343TyrAla: 2.343 ± 0.039	0.306TyrCys: 0.306 ± 0.016	1.573TyrAsp: 1.573 ± 0.039	1.537TyrGlu: 1.537 ± 0.034	0.976TyrPhe: 0.976 ± 0.029	2.118TyrGly: 2.118 ± 0.041	0.694TyrHis: 0.694 ± 0.026	0.91TyrIle: 0.91 ± 0.029	0.799TyrLys: 0.799 ± 0.027	2.78TyrLeu: 2.78 ± 0.047	0.446TyrMet: 0.446 ± 0.02	0.648TyrAsn: 0.648 ± 0.023	1.354TyrPro: 1.354 ± 0.032	0.984TyrGln: 0.984 ± 0.026	2.449TyrArg: 2.449 ± 0.046	1.191TyrSer: 1.191 ± 0.03	1.168TyrThr: 1.168 ± 0.035	1.838TyrVal: 1.838 ± 0.039	0.427TyrTrp: 0.427 ± 0.018	0.851TyrTyr: 0.851 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4733 proteins (1377880 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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