Luffa begomovirus betasatellite
Average proteome isoelectric point is 5.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M4ARL0|A0A0M4ARL0_9VIRU C1 OS=Luffa begomovirus betasatellite OX=1705090 GN=C1 PE=4 SV=1
MM1 pKa = 7.89 DD2 pKa = 5.41 HH3 pKa = 6.63 SQPHH7 pKa = 6.51 NNTKK11 pKa = 10.49 YY12 pKa = 9.85 LTFRR16 pKa = 11.84 SINAGIMINSITQAEE31 pKa = 4.44 MTRR34 pKa = 11.84 SGTNKK39 pKa = 9.62 QGVKK43 pKa = 8.4 FTVDD47 pKa = 3.1 VRR49 pKa = 11.84 IMEE52 pKa = 3.99 NMKK55 pKa = 10.08 IFIHH59 pKa = 6.17 IRR61 pKa = 11.84 IISTMSPALIKK72 pKa = 10.99 YY73 pKa = 8.87 EE74 pKa = 4.49 GIVKK78 pKa = 8.12 YY79 pKa = 9.0 TYY81 pKa = 11.57 GDD83 pKa = 3.39 MQVPFDD89 pKa = 4.14 FNGFEE94 pKa = 4.42 GNIIANFLFANNGAKK109 pKa = 9.48 IEE111 pKa = 4.34 EE112 pKa = 4.35 IEE114 pKa = 4.88 IEE116 pKa = 4.88 DD117 pKa = 3.24 IVQRR121 pKa = 11.84 LDD123 pKa = 3.24 ILVLEE128 pKa = 4.49 NPEE131 pKa = 4.0 ILGMDD136 pKa = 3.91 VIEE139 pKa = 4.67 PYY141 pKa = 10.87 VFNKK145 pKa = 10.0 KK146 pKa = 8.02 FTVV149 pKa = 3.26
Molecular weight: 17.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.25
IPC2_protein 5.321
IPC_protein 5.207
Toseland 5.258
ProMoST 5.385
Dawson 5.245
Bjellqvist 5.347
Wikipedia 5.118
Rodwell 5.181
Grimsley 5.207
Solomon 5.245
Lehninger 5.207
Nozaki 5.385
DTASelect 5.512
Thurlkill 5.27
EMBOSS 5.207
Sillero 5.461
Patrickios 4.24
IPC_peptide 5.245
IPC2_peptide 5.461
IPC2.peptide.svr19 5.486
Protein with the highest isoelectric point:
>tr|A0A0M4ARL0|A0A0M4ARL0_9VIRU C1 OS=Luffa begomovirus betasatellite OX=1705090 GN=C1 PE=4 SV=1
MM1 pKa = 7.89 DD2 pKa = 5.41 HH3 pKa = 6.63 SQPHH7 pKa = 6.51 NNTKK11 pKa = 10.49 YY12 pKa = 9.85 LTFRR16 pKa = 11.84 SINAGIMINSITQAEE31 pKa = 4.44 MTRR34 pKa = 11.84 SGTNKK39 pKa = 9.62 QGVKK43 pKa = 8.4 FTVDD47 pKa = 3.1 VRR49 pKa = 11.84 IMEE52 pKa = 3.99 NMKK55 pKa = 10.08 IFIHH59 pKa = 6.17 IRR61 pKa = 11.84 IISTMSPALIKK72 pKa = 10.99 YY73 pKa = 8.87 EE74 pKa = 4.49 GIVKK78 pKa = 8.12 YY79 pKa = 9.0 TYY81 pKa = 11.57 GDD83 pKa = 3.39 MQVPFDD89 pKa = 4.14 FNGFEE94 pKa = 4.42 GNIIANFLFANNGAKK109 pKa = 9.48 IEE111 pKa = 4.34 EE112 pKa = 4.35 IEE114 pKa = 4.88 IEE116 pKa = 4.88 DD117 pKa = 3.24 IVQRR121 pKa = 11.84 LDD123 pKa = 3.24 ILVLEE128 pKa = 4.49 NPEE131 pKa = 4.0 ILGMDD136 pKa = 3.91 VIEE139 pKa = 4.67 PYY141 pKa = 10.87 VFNKK145 pKa = 10.0 KK146 pKa = 8.02 FTVV149 pKa = 3.26
Molecular weight: 17.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.25
IPC2_protein 5.321
IPC_protein 5.207
Toseland 5.258
ProMoST 5.385
Dawson 5.245
Bjellqvist 5.347
Wikipedia 5.118
Rodwell 5.181
Grimsley 5.207
Solomon 5.245
Lehninger 5.207
Nozaki 5.385
DTASelect 5.512
Thurlkill 5.27
EMBOSS 5.207
Sillero 5.461
Patrickios 4.24
IPC_peptide 5.245
IPC2_peptide 5.461
IPC2.peptide.svr19 5.486
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
149
149
149
149.0
17.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.027 ± 0.0
0.0 ± 0.0
4.698 ± 0.0
7.383 ± 0.0
6.711 ± 0.0
6.04 ± 0.0
2.013 ± 0.0
14.094 ± 0.0
6.04 ± 0.0
4.698 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.369 ± 0.0
8.725 ± 0.0
3.356 ± 0.0
3.356 ± 0.0
3.356 ± 0.0
4.027 ± 0.0
6.04 ± 0.0
6.711 ± 0.0
0.0 ± 0.0
3.356 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here