Sclerotinia sclerotiorum mitovirus 6
Average proteome isoelectric point is 8.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A023J4K6|A0A023J4K6_9VIRU Pol OS=Sclerotinia sclerotiorum mitovirus 6 OX=1435446 PE=4 SV=1
MM1 pKa = 7.76 KK2 pKa = 10.23 KK3 pKa = 8.1 QTYY6 pKa = 9.9 KK7 pKa = 10.69 LIKK10 pKa = 9.05 IICMLFLNIGRR21 pKa = 11.84 HH22 pKa = 4.56 TYY24 pKa = 10.71 VIGLVMKK31 pKa = 10.5 RR32 pKa = 11.84 IHH34 pKa = 6.72 NLLKK38 pKa = 11.15 NNGTLFTVKK47 pKa = 10.21 YY48 pKa = 9.35 LKK50 pKa = 9.76 QARR53 pKa = 11.84 LHH55 pKa = 4.39 VTRR58 pKa = 11.84 YY59 pKa = 9.1 YY60 pKa = 10.67 CGSPLMEE67 pKa = 4.48 NNIGVSLTQTGFPIIFKK84 pKa = 10.12 EE85 pKa = 4.15 CEE87 pKa = 3.84 PLIKK91 pKa = 10.65 GSMNLKK97 pKa = 10.18 KK98 pKa = 10.63 FVFTLINISRR108 pKa = 11.84 TIRR111 pKa = 11.84 PRR113 pKa = 11.84 KK114 pKa = 10.09 GEE116 pKa = 4.34 IIPISLKK123 pKa = 10.66 PITDD127 pKa = 3.67 PSTAVINKK135 pKa = 9.5 LDD137 pKa = 3.5 NRR139 pKa = 11.84 LLRR142 pKa = 11.84 KK143 pKa = 9.93 VISQNDD149 pKa = 3.31 ICLSIPDD156 pKa = 4.74 HH157 pKa = 6.25 GLSDD161 pKa = 4.92 LEE163 pKa = 4.48 LTTKK167 pKa = 10.41 SGPHH171 pKa = 6.31 GPQTLTALYY180 pKa = 10.7 SMKK183 pKa = 10.28 RR184 pKa = 11.84 YY185 pKa = 8.24 STATASAIMGMLSTSLFKK203 pKa = 11.0 YY204 pKa = 10.5 FKK206 pKa = 10.57 DD207 pKa = 3.67 LYY209 pKa = 10.37 QYY211 pKa = 11.3 SLSKK215 pKa = 10.14 PDD217 pKa = 3.36 EE218 pKa = 4.23 VKK220 pKa = 10.78 PCGCKK225 pKa = 9.68 AAPEE229 pKa = 4.0 VRR231 pKa = 11.84 RR232 pKa = 11.84 LSIVKK237 pKa = 10.3 DD238 pKa = 3.94 PEE240 pKa = 3.84 CKK242 pKa = 9.42 MRR244 pKa = 11.84 VIGMFDD250 pKa = 2.67 WWSQVTLKK258 pKa = 10.49 PLSDD262 pKa = 4.07 SLFKK266 pKa = 10.9 ALSKK270 pKa = 10.17 IDD272 pKa = 3.34 SDD274 pKa = 3.51 RR275 pKa = 11.84 TYY277 pKa = 10.72 SQDD280 pKa = 3.36 PAFHH284 pKa = 6.92 FEE286 pKa = 3.91 VDD288 pKa = 3.83 TNLLWSIDD296 pKa = 3.47 LTAATDD302 pKa = 3.81 RR303 pKa = 11.84 FPIEE307 pKa = 3.95 LQKK310 pKa = 10.99 QILSILTNTAFTRR323 pKa = 11.84 DD324 pKa = 3.32 WADD327 pKa = 3.0 VMVGEE332 pKa = 4.36 EE333 pKa = 3.97 FSYY336 pKa = 11.01 EE337 pKa = 4.02 GGSISYY343 pKa = 10.22 RR344 pKa = 11.84 VGQPMGAYY352 pKa = 9.93 SSWPMFTLSHH362 pKa = 6.87 HH363 pKa = 5.92 ILVRR367 pKa = 11.84 YY368 pKa = 9.74 CGLLNGIDD376 pKa = 3.5 NFNRR380 pKa = 11.84 YY381 pKa = 8.65 IMLGDD386 pKa = 5.05 DD387 pKa = 4.19 IVINHH392 pKa = 6.18 NKK394 pKa = 8.37 VAKK397 pKa = 9.28 TYY399 pKa = 10.38 IRR401 pKa = 11.84 LLHH404 pKa = 6.22 ALGVEE409 pKa = 4.66 TSQAKK414 pKa = 6.99 THH416 pKa = 5.39 VSKK419 pKa = 9.2 HH420 pKa = 4.6 TYY422 pKa = 9.7 EE423 pKa = 4.18 FAKK426 pKa = 10.17 RR427 pKa = 11.84 WIDD430 pKa = 3.51 TRR432 pKa = 11.84 CGEE435 pKa = 4.16 ITGLPIKK442 pKa = 10.62 GLLDD446 pKa = 4.13 NIEE449 pKa = 4.08 NMSTCFQILFDD460 pKa = 4.03 WQLKK464 pKa = 8.97 GNKK467 pKa = 8.7 FWTDD471 pKa = 2.83 RR472 pKa = 11.84 SLVEE476 pKa = 4.17 SVADD480 pKa = 3.63 FLYY483 pKa = 10.53 KK484 pKa = 10.69 VQDD487 pKa = 4.21 FSKK490 pKa = 10.7 KK491 pKa = 8.34 PYY493 pKa = 9.57 GYY495 pKa = 10.48 RR496 pKa = 11.84 KK497 pKa = 9.4 ILEE500 pKa = 4.19 TLKK503 pKa = 10.64 PISFFMRR510 pKa = 11.84 LRR512 pKa = 11.84 FSLSTPDD519 pKa = 3.17 EE520 pKa = 4.17 VRR522 pKa = 11.84 NQICLWTRR530 pKa = 11.84 NSQFIVGGAEE540 pKa = 3.78 KK541 pKa = 10.76 LILNEE546 pKa = 3.72 VLRR549 pKa = 11.84 VFNLTYY555 pKa = 10.41 SALVQKK561 pKa = 10.35 SSRR564 pKa = 11.84 LIVEE568 pKa = 5.18 FYY570 pKa = 10.79 HH571 pKa = 7.33 RR572 pKa = 11.84 INEE575 pKa = 4.21 FVEE578 pKa = 4.5 LEE580 pKa = 4.01 DD581 pKa = 5.7 NIIEE585 pKa = 4.32 DD586 pKa = 3.96 HH587 pKa = 5.8 VHH589 pKa = 7.46 PILQCIEE596 pKa = 3.83 NHH598 pKa = 6.19 LEE600 pKa = 4.24 RR601 pKa = 11.84 IAEE604 pKa = 4.12 KK605 pKa = 10.63 QEE607 pKa = 4.0 SQRR610 pKa = 11.84 GDD612 pKa = 3.27 LTLKK616 pKa = 10.12 EE617 pKa = 4.16 MCDD620 pKa = 3.6 TLMLMDD626 pKa = 4.98 IEE628 pKa = 5.82 SIMTQKK634 pKa = 8.98 RR635 pKa = 11.84 TKK637 pKa = 9.24 VQIMVNNTKK646 pKa = 9.36 MAHH649 pKa = 6.01 KK650 pKa = 9.87 FVNLVKK656 pKa = 10.52 SWDD659 pKa = 3.47 HH660 pKa = 6.62 DD661 pKa = 3.72 NPRR664 pKa = 11.84 FSDD667 pKa = 3.6 PQNFFHH673 pKa = 7.3 GGAADD678 pKa = 3.21 IGFGSTSVQQTLDD691 pKa = 2.67 RR692 pKa = 11.84 FRR694 pKa = 11.84 RR695 pKa = 11.84 YY696 pKa = 9.47 FLL698 pKa = 4.27
Molecular weight: 80.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.506
IPC2_protein 8.404
IPC_protein 8.258
Toseland 8.829
ProMoST 8.829
Dawson 9.224
Bjellqvist 9.151
Wikipedia 9.355
Rodwell 9.414
Grimsley 9.18
Solomon 9.268
Lehninger 9.238
Nozaki 9.311
DTASelect 9.019
Thurlkill 9.151
EMBOSS 9.37
Sillero 9.326
Patrickios 4.647
IPC_peptide 9.268
IPC2_peptide 7.863
IPC2.peptide.svr19 7.72
Protein with the highest isoelectric point:
>tr|A0A023J4K6|A0A023J4K6_9VIRU Pol OS=Sclerotinia sclerotiorum mitovirus 6 OX=1435446 PE=4 SV=1
MM1 pKa = 7.76 KK2 pKa = 10.23 KK3 pKa = 8.1 QTYY6 pKa = 9.9 KK7 pKa = 10.69 LIKK10 pKa = 9.05 IICMLFLNIGRR21 pKa = 11.84 HH22 pKa = 4.56 TYY24 pKa = 10.71 VIGLVMKK31 pKa = 10.5 RR32 pKa = 11.84 IHH34 pKa = 6.72 NLLKK38 pKa = 11.15 NNGTLFTVKK47 pKa = 10.21 YY48 pKa = 9.35 LKK50 pKa = 9.76 QARR53 pKa = 11.84 LHH55 pKa = 4.39 VTRR58 pKa = 11.84 YY59 pKa = 9.1 YY60 pKa = 10.67 CGSPLMEE67 pKa = 4.48 NNIGVSLTQTGFPIIFKK84 pKa = 10.12 EE85 pKa = 4.15 CEE87 pKa = 3.84 PLIKK91 pKa = 10.65 GSMNLKK97 pKa = 10.18 KK98 pKa = 10.63 FVFTLINISRR108 pKa = 11.84 TIRR111 pKa = 11.84 PRR113 pKa = 11.84 KK114 pKa = 10.09 GEE116 pKa = 4.34 IIPISLKK123 pKa = 10.66 PITDD127 pKa = 3.67 PSTAVINKK135 pKa = 9.5 LDD137 pKa = 3.5 NRR139 pKa = 11.84 LLRR142 pKa = 11.84 KK143 pKa = 9.93 VISQNDD149 pKa = 3.31 ICLSIPDD156 pKa = 4.74 HH157 pKa = 6.25 GLSDD161 pKa = 4.92 LEE163 pKa = 4.48 LTTKK167 pKa = 10.41 SGPHH171 pKa = 6.31 GPQTLTALYY180 pKa = 10.7 SMKK183 pKa = 10.28 RR184 pKa = 11.84 YY185 pKa = 8.24 STATASAIMGMLSTSLFKK203 pKa = 11.0 YY204 pKa = 10.5 FKK206 pKa = 10.57 DD207 pKa = 3.67 LYY209 pKa = 10.37 QYY211 pKa = 11.3 SLSKK215 pKa = 10.14 PDD217 pKa = 3.36 EE218 pKa = 4.23 VKK220 pKa = 10.78 PCGCKK225 pKa = 9.68 AAPEE229 pKa = 4.0 VRR231 pKa = 11.84 RR232 pKa = 11.84 LSIVKK237 pKa = 10.3 DD238 pKa = 3.94 PEE240 pKa = 3.84 CKK242 pKa = 9.42 MRR244 pKa = 11.84 VIGMFDD250 pKa = 2.67 WWSQVTLKK258 pKa = 10.49 PLSDD262 pKa = 4.07 SLFKK266 pKa = 10.9 ALSKK270 pKa = 10.17 IDD272 pKa = 3.34 SDD274 pKa = 3.51 RR275 pKa = 11.84 TYY277 pKa = 10.72 SQDD280 pKa = 3.36 PAFHH284 pKa = 6.92 FEE286 pKa = 3.91 VDD288 pKa = 3.83 TNLLWSIDD296 pKa = 3.47 LTAATDD302 pKa = 3.81 RR303 pKa = 11.84 FPIEE307 pKa = 3.95 LQKK310 pKa = 10.99 QILSILTNTAFTRR323 pKa = 11.84 DD324 pKa = 3.32 WADD327 pKa = 3.0 VMVGEE332 pKa = 4.36 EE333 pKa = 3.97 FSYY336 pKa = 11.01 EE337 pKa = 4.02 GGSISYY343 pKa = 10.22 RR344 pKa = 11.84 VGQPMGAYY352 pKa = 9.93 SSWPMFTLSHH362 pKa = 6.87 HH363 pKa = 5.92 ILVRR367 pKa = 11.84 YY368 pKa = 9.74 CGLLNGIDD376 pKa = 3.5 NFNRR380 pKa = 11.84 YY381 pKa = 8.65 IMLGDD386 pKa = 5.05 DD387 pKa = 4.19 IVINHH392 pKa = 6.18 NKK394 pKa = 8.37 VAKK397 pKa = 9.28 TYY399 pKa = 10.38 IRR401 pKa = 11.84 LLHH404 pKa = 6.22 ALGVEE409 pKa = 4.66 TSQAKK414 pKa = 6.99 THH416 pKa = 5.39 VSKK419 pKa = 9.2 HH420 pKa = 4.6 TYY422 pKa = 9.7 EE423 pKa = 4.18 FAKK426 pKa = 10.17 RR427 pKa = 11.84 WIDD430 pKa = 3.51 TRR432 pKa = 11.84 CGEE435 pKa = 4.16 ITGLPIKK442 pKa = 10.62 GLLDD446 pKa = 4.13 NIEE449 pKa = 4.08 NMSTCFQILFDD460 pKa = 4.03 WQLKK464 pKa = 8.97 GNKK467 pKa = 8.7 FWTDD471 pKa = 2.83 RR472 pKa = 11.84 SLVEE476 pKa = 4.17 SVADD480 pKa = 3.63 FLYY483 pKa = 10.53 KK484 pKa = 10.69 VQDD487 pKa = 4.21 FSKK490 pKa = 10.7 KK491 pKa = 8.34 PYY493 pKa = 9.57 GYY495 pKa = 10.48 RR496 pKa = 11.84 KK497 pKa = 9.4 ILEE500 pKa = 4.19 TLKK503 pKa = 10.64 PISFFMRR510 pKa = 11.84 LRR512 pKa = 11.84 FSLSTPDD519 pKa = 3.17 EE520 pKa = 4.17 VRR522 pKa = 11.84 NQICLWTRR530 pKa = 11.84 NSQFIVGGAEE540 pKa = 3.78 KK541 pKa = 10.76 LILNEE546 pKa = 3.72 VLRR549 pKa = 11.84 VFNLTYY555 pKa = 10.41 SALVQKK561 pKa = 10.35 SSRR564 pKa = 11.84 LIVEE568 pKa = 5.18 FYY570 pKa = 10.79 HH571 pKa = 7.33 RR572 pKa = 11.84 INEE575 pKa = 4.21 FVEE578 pKa = 4.5 LEE580 pKa = 4.01 DD581 pKa = 5.7 NIIEE585 pKa = 4.32 DD586 pKa = 3.96 HH587 pKa = 5.8 VHH589 pKa = 7.46 PILQCIEE596 pKa = 3.83 NHH598 pKa = 6.19 LEE600 pKa = 4.24 RR601 pKa = 11.84 IAEE604 pKa = 4.12 KK605 pKa = 10.63 QEE607 pKa = 4.0 SQRR610 pKa = 11.84 GDD612 pKa = 3.27 LTLKK616 pKa = 10.12 EE617 pKa = 4.16 MCDD620 pKa = 3.6 TLMLMDD626 pKa = 4.98 IEE628 pKa = 5.82 SIMTQKK634 pKa = 8.98 RR635 pKa = 11.84 TKK637 pKa = 9.24 VQIMVNNTKK646 pKa = 9.36 MAHH649 pKa = 6.01 KK650 pKa = 9.87 FVNLVKK656 pKa = 10.52 SWDD659 pKa = 3.47 HH660 pKa = 6.62 DD661 pKa = 3.72 NPRR664 pKa = 11.84 FSDD667 pKa = 3.6 PQNFFHH673 pKa = 7.3 GGAADD678 pKa = 3.21 IGFGSTSVQQTLDD691 pKa = 2.67 RR692 pKa = 11.84 FRR694 pKa = 11.84 RR695 pKa = 11.84 YY696 pKa = 9.47 FLL698 pKa = 4.27
Molecular weight: 80.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.506
IPC2_protein 8.404
IPC_protein 8.258
Toseland 8.829
ProMoST 8.829
Dawson 9.224
Bjellqvist 9.151
Wikipedia 9.355
Rodwell 9.414
Grimsley 9.18
Solomon 9.268
Lehninger 9.238
Nozaki 9.311
DTASelect 9.019
Thurlkill 9.151
EMBOSS 9.37
Sillero 9.326
Patrickios 4.647
IPC_peptide 9.268
IPC2_peptide 7.863
IPC2.peptide.svr19 7.72
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
698
698
698
698.0
80.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.725 ± 0.0
1.862 ± 0.0
5.444 ± 0.0
4.728 ± 0.0
5.301 ± 0.0
5.014 ± 0.0
2.722 ± 0.0
8.166 ± 0.0
7.307 ± 0.0
10.745 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.152 ± 0.0
4.728 ± 0.0
3.725 ± 0.0
3.725 ± 0.0
5.444 ± 0.0
7.307 ± 0.0
6.59 ± 0.0
5.444 ± 0.0
1.433 ± 0.0
3.438 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here