Azoarcus pumilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Azoarcus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3041 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6S3A3|A0A2I6S3A3_9RHOO FecR domain-containing protein OS=Azoarcus pumilus OX=2067960 GN=C0099_01545 PE=4 SV=1
MM1 pKa = 7.98IGIEE5 pKa = 4.22LLQGAARR12 pKa = 11.84RR13 pKa = 11.84EE14 pKa = 4.24SGRR17 pKa = 11.84GVNARR22 pKa = 11.84SAARR26 pKa = 11.84RR27 pKa = 11.84LLAVLRR33 pKa = 11.84GLIFVPGLVLAAGTAMAQNVDD54 pKa = 3.57LVLNHH59 pKa = 7.33DD60 pKa = 4.19LTSAATINASDD71 pKa = 3.53TAAFTVTVDD80 pKa = 3.75NSDD83 pKa = 3.5PTHH86 pKa = 6.57TGDD89 pKa = 3.5ANNVVVTYY97 pKa = 9.91QISATDD103 pKa = 3.85TLLSASPSQGSCSLPPDD120 pKa = 3.59ASNTFTCNFGTIPHH134 pKa = 5.84TTNATLDD141 pKa = 3.67VVVRR145 pKa = 11.84ATGTGVGSFPYY156 pKa = 10.73TMTTNAAVATSSTDD170 pKa = 3.35TEE172 pKa = 4.1ATNNDD177 pKa = 3.37EE178 pKa = 4.22EE179 pKa = 4.57RR180 pKa = 11.84TVTVQEE186 pKa = 4.33GTDD189 pKa = 3.29VGIVLNSSPGAIASGGIWTHH209 pKa = 5.33EE210 pKa = 3.84MVVTNHH216 pKa = 5.72GPIGATNIVAHH227 pKa = 7.06LDD229 pKa = 3.73LPAGAILVGSLPAGCSAGAPGVVCQIASLALSGVQNIGPITTQIAATSGSNLSATALVDD288 pKa = 5.08SLDD291 pKa = 3.72QYY293 pKa = 11.88DD294 pKa = 4.51GNTTNQSSTRR304 pKa = 11.84QVDD307 pKa = 3.67VNPGSDD313 pKa = 3.26LAVTLGQSGGPFLEE327 pKa = 4.4NEE329 pKa = 4.25PFALTANPSYY339 pKa = 10.17TGEE342 pKa = 4.2VPDD345 pKa = 4.19VPEE348 pKa = 4.64LSISVDD354 pKa = 3.08PGLQIQNPAVFNQNGWTCSVSGQLVSCSGLDD385 pKa = 3.57TSGMTAGANQPLGVVTVNVVGDD407 pKa = 3.53TAGTYY412 pKa = 9.48SANEE416 pKa = 3.94ATVSADD422 pKa = 3.44YY423 pKa = 10.54TNKK426 pKa = 9.74PDD428 pKa = 4.37PNLDD432 pKa = 3.54NNIDD436 pKa = 3.6GTAPAIVAAEE446 pKa = 4.1LNLGIVKK453 pKa = 10.14SAPQRR458 pKa = 11.84DD459 pKa = 3.91LRR461 pKa = 11.84TIGAAYY467 pKa = 9.18PFDD470 pKa = 3.94YY471 pKa = 10.89SVTVRR476 pKa = 11.84NNGNVGFWGDD486 pKa = 3.93LTWTDD491 pKa = 3.94TVPEE495 pKa = 4.58GLTITNIAAPGGWTCTTVPPNVVGADD521 pKa = 3.22TFTCSRR527 pKa = 11.84TYY529 pKa = 11.43AEE531 pKa = 4.52GAPFAVNATQAFTITAFANGDD552 pKa = 3.41IVGDD556 pKa = 4.08LVNGACLTTIDD567 pKa = 4.43HH568 pKa = 7.51DD569 pKa = 4.86LGFLEE574 pKa = 5.28NEE576 pKa = 4.26VATPCSNATINAQTPGSSADD596 pKa = 3.7LLVEE600 pKa = 4.36KK601 pKa = 9.13TASAATVNAGEE612 pKa = 4.26TLTYY616 pKa = 8.97TLRR619 pKa = 11.84IVNDD623 pKa = 4.38GPATSTSVTLTDD635 pKa = 3.41VLSNLFTNSGGNSFQGVTVNNGTASGGSCGSSGNVGNPSSRR676 pKa = 11.84TLSCTFTSIPVCSGGDD692 pKa = 3.74CPTVTVTILPHH703 pKa = 6.31GNSADD708 pKa = 3.53GTALTRR714 pKa = 11.84GNTASAYY721 pKa = 9.57SSVVADD727 pKa = 3.96PDD729 pKa = 3.81YY730 pKa = 11.78SNNTDD735 pKa = 3.48NVDD738 pKa = 3.36VSIDD742 pKa = 3.58ALTDD746 pKa = 3.58LALSKK751 pKa = 9.68TVTTWSGEE759 pKa = 3.73LGTPLNYY766 pKa = 10.18RR767 pKa = 11.84IQVTNEE773 pKa = 3.73GASGASSLTMTDD785 pKa = 3.56VLPHH789 pKa = 7.17DD790 pKa = 4.07LTYY793 pKa = 11.24LSVNPGGGASCATQPTANSTTEE815 pKa = 3.62ASNDD819 pKa = 3.39QIICTRR825 pKa = 11.84STLARR830 pKa = 11.84GATWTIDD837 pKa = 3.05VATRR841 pKa = 11.84PNHH844 pKa = 6.39GIAPGSTLVNNAVVTTDD861 pKa = 3.45TPEE864 pKa = 4.04TTSDD868 pKa = 3.62NNADD872 pKa = 3.63TADD875 pKa = 3.88AEE877 pKa = 4.36VGEE880 pKa = 4.5ASVDD884 pKa = 3.74LAVIKK889 pKa = 10.59SDD891 pKa = 3.38TPDD894 pKa = 3.38PVFVADD900 pKa = 3.66TVTYY904 pKa = 8.76TVQIINNGPSVASAATIYY922 pKa = 11.0DD923 pKa = 3.99FLPSEE928 pKa = 4.38GFRR931 pKa = 11.84FVEE934 pKa = 5.26DD935 pKa = 3.67SITYY939 pKa = 8.52YY940 pKa = 10.69DD941 pKa = 3.35VSGGVFTEE949 pKa = 4.33IDD951 pKa = 3.69PGDD954 pKa = 3.67LAGLGIACSKK964 pKa = 10.78EE965 pKa = 3.8PDD967 pKa = 3.64DD968 pKa = 4.0EE969 pKa = 5.58AFGTGYY975 pKa = 9.7PDD977 pKa = 3.53QPLDD981 pKa = 5.49DD982 pKa = 4.56SYY984 pKa = 11.36LWPMDD989 pKa = 3.48TVEE992 pKa = 4.78NPDD995 pKa = 3.68YY996 pKa = 11.26LDD998 pKa = 4.5GVWDD1002 pKa = 4.22PLQGDD1007 pKa = 4.09LVEE1010 pKa = 5.7DD1011 pKa = 4.32ADD1013 pKa = 5.34LICNMGLLLNGQIRR1027 pKa = 11.84AIRR1030 pKa = 11.84YY1031 pKa = 7.25EE1032 pKa = 4.05LEE1034 pKa = 4.13ADD1036 pKa = 3.26TRR1038 pKa = 11.84GVYY1041 pKa = 10.26FNYY1044 pKa = 10.44AISRR1048 pKa = 11.84AQEE1051 pKa = 3.87HH1052 pKa = 7.06RR1053 pKa = 11.84DD1054 pKa = 3.54NGADD1058 pKa = 3.73GPDD1061 pKa = 3.58PVPTNDD1067 pKa = 2.87VTRR1070 pKa = 11.84EE1071 pKa = 3.75RR1072 pKa = 11.84TTVRR1076 pKa = 11.84SVPDD1080 pKa = 3.06VSITKK1085 pKa = 9.38VASSSLVSVMEE1096 pKa = 4.3PFDD1099 pKa = 4.11FVITFTNHH1107 pKa = 5.97HH1108 pKa = 5.9ATEE1111 pKa = 3.99PAYY1114 pKa = 10.36FPEE1117 pKa = 5.22IRR1119 pKa = 11.84DD1120 pKa = 3.61VLPAGMEE1127 pKa = 4.05LTGAPILVSGAPGGSVCTGAAGDD1150 pKa = 4.04EE1151 pKa = 4.24SFEE1154 pKa = 4.49CSFGSGLPPGQSVTVSVPVWVVSDD1178 pKa = 3.74GAGPRR1183 pKa = 11.84TNRR1186 pKa = 11.84VEE1188 pKa = 4.06LHH1190 pKa = 6.97LDD1192 pKa = 3.09TDD1194 pKa = 4.41LEE1196 pKa = 4.34FDD1198 pKa = 3.9EE1199 pKa = 5.54PPPPVAEE1206 pKa = 5.13DD1207 pKa = 3.84EE1208 pKa = 4.45DD1209 pKa = 4.6TVDD1212 pKa = 4.07VVISSLAGRR1221 pKa = 11.84VYY1223 pKa = 10.79HH1224 pKa = 7.26DD1225 pKa = 3.55NDD1227 pKa = 3.38YY1228 pKa = 11.56SGVHH1232 pKa = 5.17TVGNPPIEE1240 pKa = 4.27NVLITLNGVSTWGDD1254 pKa = 3.35PVTRR1258 pKa = 11.84SATTAPDD1265 pKa = 3.32GTYY1268 pKa = 10.56FFGDD1272 pKa = 4.34LPSGLYY1278 pKa = 9.26TVTQTQPGGWIDD1290 pKa = 4.08GPVNPGSVGGVPVTNEE1306 pKa = 3.45IGSIEE1311 pKa = 4.28LPADD1315 pKa = 3.48TDD1317 pKa = 3.66AVQYY1321 pKa = 11.4NFGEE1325 pKa = 4.36YY1326 pKa = 10.14LDD1328 pKa = 4.64SDD1330 pKa = 4.19SGQLASVAGHH1340 pKa = 6.69VYY1342 pKa = 10.68HH1343 pKa = 7.55DD1344 pKa = 3.91ANRR1347 pKa = 11.84NGVFDD1352 pKa = 4.55DD1353 pKa = 4.55AEE1355 pKa = 4.33SGIADD1360 pKa = 3.33VVVTLWRR1367 pKa = 11.84DD1368 pKa = 3.32GAVVATALTDD1378 pKa = 3.24AGGAYY1383 pKa = 10.42FFGQLAPGTYY1393 pKa = 9.8RR1394 pKa = 11.84ITEE1397 pKa = 3.91THH1399 pKa = 6.51PDD1401 pKa = 3.04GWIDD1405 pKa = 3.29GAEE1408 pKa = 3.99AVGVGATEE1416 pKa = 4.49PGVSPEE1422 pKa = 3.77TDD1424 pKa = 3.03VFEE1427 pKa = 5.7SIVLQGGDD1435 pKa = 3.04AAQNYY1440 pKa = 10.03DD1441 pKa = 3.27FGEE1444 pKa = 4.31YY1445 pKa = 9.39TSQDD1449 pKa = 2.96IAPIPTLQFKK1459 pKa = 11.06AMLLLILLMGVYY1471 pKa = 10.58AMYY1474 pKa = 10.48RR1475 pKa = 11.84RR1476 pKa = 11.84RR1477 pKa = 11.84EE1478 pKa = 3.91VV1479 pKa = 2.87

Molecular weight: 152.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6SAJ6|A0A2I6SAJ6_9RHOO Uncharacterized protein OS=Azoarcus pumilus OX=2067960 GN=C0099_00560 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.6RR14 pKa = 11.84THH16 pKa = 5.75GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3041

0

3041

971649

28

1859

319.5

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.797 ± 0.056

1.01 ± 0.015

5.93 ± 0.035

6.245 ± 0.036

3.616 ± 0.026

8.278 ± 0.041

2.41 ± 0.022

4.69 ± 0.029

2.452 ± 0.032

10.672 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.021

2.334 ± 0.023

4.976 ± 0.03

3.023 ± 0.02

8.169 ± 0.049

4.895 ± 0.027

4.769 ± 0.028

7.796 ± 0.035

1.393 ± 0.017

2.127 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski