Apis mellifera associated microvirus 54

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5W7|A0A3Q8U5W7_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 54 OX=2494785 PE=4 SV=1
MM1 pKa = 7.4SRR3 pKa = 11.84RR4 pKa = 11.84EE5 pKa = 4.01QVSDD9 pKa = 3.62QPITASLKK17 pKa = 9.97FKK19 pKa = 10.5HH20 pKa = 6.07KK21 pKa = 10.64QVDD24 pKa = 3.84TLSEE28 pKa = 3.88LMQRR32 pKa = 11.84LSMVAKK38 pKa = 8.83EE39 pKa = 4.5KK40 pKa = 10.52EE41 pKa = 4.15YY42 pKa = 9.88EE43 pKa = 4.21TLEE46 pKa = 4.14EE47 pKa = 5.12ADD49 pKa = 4.86DD50 pKa = 4.2FNIGDD55 pKa = 4.88DD56 pKa = 4.39YY57 pKa = 11.78DD58 pKa = 4.35PSSPWEE64 pKa = 3.99EE65 pKa = 3.84QFDD68 pKa = 4.08PQGEE72 pKa = 4.52SLGFLATGIKK82 pKa = 10.09KK83 pKa = 9.68PPQGGEE89 pKa = 3.84PQAKK93 pKa = 9.72EE94 pKa = 4.38GEE96 pKa = 4.27NPPAEE101 pKa = 3.98PSIDD105 pKa = 3.91KK106 pKa = 11.0NDD108 pKa = 3.38LL109 pKa = 3.49

Molecular weight: 12.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8U5M1|A0A3Q8U5M1_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 54 OX=2494785 PE=4 SV=1
MM1 pKa = 7.66AKK3 pKa = 9.62SQRR6 pKa = 11.84RR7 pKa = 11.84TKK9 pKa = 10.79RR10 pKa = 11.84NLKK13 pKa = 9.27PLYY16 pKa = 10.1RR17 pKa = 11.84RR18 pKa = 11.84DD19 pKa = 3.28AVAIARR25 pKa = 11.84AVPRR29 pKa = 11.84EE30 pKa = 4.06DD31 pKa = 3.22NALPEE36 pKa = 4.34VYY38 pKa = 10.48LLRR41 pKa = 11.84NLPYY45 pKa = 10.81VNYY48 pKa = 7.47TTSTPYY54 pKa = 9.42QSKK57 pKa = 10.56IIIDD61 pKa = 4.05DD62 pKa = 3.67NRR64 pKa = 11.84SYY66 pKa = 11.57NPDD69 pKa = 3.2PTRR72 pKa = 11.84PPLTISGNYY81 pKa = 9.11SRR83 pKa = 11.84VIAPTHH89 pKa = 5.61RR90 pKa = 11.84NIRR93 pKa = 11.84SSRR96 pKa = 11.84RR97 pKa = 11.84NISGIKK103 pKa = 8.86RR104 pKa = 11.84TFPSYY109 pKa = 11.09KK110 pKa = 9.99LRR112 pKa = 11.84LAYY115 pKa = 9.43PEE117 pKa = 3.95KK118 pKa = 10.43TIVCLRR124 pKa = 11.84RR125 pKa = 11.84NRR127 pKa = 11.84RR128 pKa = 11.84KK129 pKa = 9.75EE130 pKa = 3.82VIHH133 pKa = 6.59AKK135 pKa = 10.33GIAGSRR141 pKa = 11.84VAKK144 pKa = 10.13PKK146 pKa = 10.75FNINSQLHH154 pKa = 5.61CRR156 pKa = 3.96

Molecular weight: 18.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1349

109

490

269.8

30.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.228 ± 0.704

0.667 ± 0.308

4.967 ± 0.719

5.56 ± 0.788

3.262 ± 0.439

7.635 ± 0.871

2.52 ± 0.359

5.486 ± 0.704

5.486 ± 1.212

8.525 ± 0.747

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.076 ± 0.529

4.522 ± 0.612

5.856 ± 0.71

4.818 ± 0.668

7.116 ± 1.032

8.08 ± 0.778

5.263 ± 0.458

4.374 ± 0.763

1.557 ± 0.362

4.003 ± 0.556
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski