Beluga whale alphaherpesvirus 1
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A286MM97|A0A286MM97_9ALPH Protein Mo1 OS=Beluga whale alphaherpesvirus 1 OX=1434720 GN=Mo1 PE=4 SV=1
MM1 pKa = 7.49 AAFVSDD7 pKa = 3.41 RR8 pKa = 11.84 WFDD11 pKa = 3.64 AYY13 pKa = 10.59 MGDD16 pKa = 3.69 PWSEE20 pKa = 3.71 PVYY23 pKa = 11.09 VSLTTPLSEE32 pKa = 4.18 SAPRR36 pKa = 11.84 GTPRR40 pKa = 11.84 RR41 pKa = 11.84 APSTGSPPPSPDD53 pKa = 3.35 PEE55 pKa = 4.19 RR56 pKa = 11.84 APPLPPDD63 pKa = 4.48 PLPPVPDD70 pKa = 4.3 SPPPPYY76 pKa = 9.65 PAAEE80 pKa = 4.25 TPPSFPLADD89 pKa = 3.69 PARR92 pKa = 11.84 GDD94 pKa = 3.77 EE95 pKa = 4.47 APGAPEE101 pKa = 4.29 DD102 pKa = 3.92 ARR104 pKa = 11.84 ITATSVEE111 pKa = 4.25 NPSYY115 pKa = 10.67 LGPLLLPAGLPDD127 pKa = 4.68 RR128 pKa = 11.84 PPAYY132 pKa = 8.12 DD133 pKa = 3.64 TLSVALPPPSYY144 pKa = 10.2 EE145 pKa = 3.68 WAIMDD150 pKa = 5.33 DD151 pKa = 4.1 LLASIAYY158 pKa = 7.5 PRR160 pKa = 11.84 LGEE163 pKa = 4.19 KK164 pKa = 9.69 EE165 pKa = 4.16 CCNVVGLAALLILIIILFGVFAYY188 pKa = 9.71 ILAGSGGGMSKK199 pKa = 10.77
Molecular weight: 20.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.112
IPC2_protein 4.126
IPC_protein 4.062
Toseland 3.872
ProMoST 4.215
Dawson 4.037
Bjellqvist 4.202
Wikipedia 3.961
Rodwell 3.897
Grimsley 3.783
Solomon 4.037
Lehninger 3.986
Nozaki 4.151
DTASelect 4.368
Thurlkill 3.91
EMBOSS 3.973
Sillero 4.177
Patrickios 3.16
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.089
Protein with the highest isoelectric point:
>tr|A0A286MM96|A0A286MM96_9ALPH Protein Mo7 OS=Beluga whale alphaherpesvirus 1 OX=1434720 GN=Mo7 PE=4 SV=1
MM1 pKa = 7.79 DD2 pKa = 5.43 RR3 pKa = 11.84 GAAGLGRR10 pKa = 11.84 AACALQVWLALGLARR25 pKa = 11.84 GVGAHH30 pKa = 6.48 TDD32 pKa = 3.57 DD33 pKa = 5.2 EE34 pKa = 5.64 IDD36 pKa = 3.45 VSEE39 pKa = 4.57 YY40 pKa = 10.33 PVYY43 pKa = 10.65 NCSLVAVRR51 pKa = 11.84 NFAGWFVPPAYY62 pKa = 10.06 AVICLVGVFGNLLVLAMFVTYY83 pKa = 10.53 AKK85 pKa = 10.11 SRR87 pKa = 11.84 CLTDD91 pKa = 3.65 VFIANMALADD101 pKa = 4.29 LLLVLTLPFWAVQHH115 pKa = 6.91 ALDD118 pKa = 4.71 DD119 pKa = 4.66 WIFSNIACKK128 pKa = 7.95 VTRR131 pKa = 11.84 GIYY134 pKa = 10.19 AINFNCGMLLLTCIGVDD151 pKa = 3.31 RR152 pKa = 11.84 YY153 pKa = 10.33 IAIVRR158 pKa = 11.84 ASTAARR164 pKa = 11.84 LRR166 pKa = 11.84 DD167 pKa = 3.64 RR168 pKa = 11.84 LRR170 pKa = 11.84 ARR172 pKa = 11.84 SGLICVGVWAVALATASGTFVFTQKK197 pKa = 10.63 YY198 pKa = 8.63 HH199 pKa = 4.82 VTNSTHH205 pKa = 5.47 VCDD208 pKa = 4.86 ARR210 pKa = 11.84 FDD212 pKa = 4.14 TTSSAPLVWRR222 pKa = 11.84 RR223 pKa = 11.84 LMLSVQLASGFFFPVVCMGVFYY245 pKa = 9.88 FLIIRR250 pKa = 11.84 RR251 pKa = 11.84 LLRR254 pKa = 11.84 SINARR259 pKa = 11.84 KK260 pKa = 8.06 HH261 pKa = 3.61 TAIRR265 pKa = 11.84 VSLAMVLVFFVCQLPHH281 pKa = 6.68 NSVLLMTAVNSHH293 pKa = 5.18 GMVVHH298 pKa = 6.35 NRR300 pKa = 11.84 TDD302 pKa = 3.09 NVTEE306 pKa = 3.99 IWCPIMRR313 pKa = 11.84 NLRR316 pKa = 11.84 YY317 pKa = 9.28 AQRR320 pKa = 11.84 ATEE323 pKa = 4.14 VLAFLHH329 pKa = 6.55 GSVNPVLYY337 pKa = 10.77 AFLGKK342 pKa = 10.21 KK343 pKa = 9.65 FRR345 pKa = 11.84 EE346 pKa = 3.88 QFLRR350 pKa = 11.84 MVRR353 pKa = 11.84 RR354 pKa = 11.84 RR355 pKa = 11.84 GAAPAMRR362 pKa = 11.84 ISSPVKK368 pKa = 9.67 RR369 pKa = 11.84 PSISPPPARR378 pKa = 11.84 PLLGAVCGPPRR389 pKa = 11.84 SPPPKK394 pKa = 9.39 TVFTFPSDD402 pKa = 3.55 GFSGPVFPLVVAFPAALPAPPHH424 pKa = 5.38 SLGWVSSPEE433 pKa = 3.83 RR434 pKa = 11.84 PGRR437 pKa = 11.84 VARR440 pKa = 11.84 PLDD443 pKa = 3.66 SAPRR447 pKa = 11.84 RR448 pKa = 11.84 ARR450 pKa = 11.84 TRR452 pKa = 11.84 GTPAAHH458 pKa = 5.8 VEE460 pKa = 4.38 KK461 pKa = 11.09 VV462 pKa = 2.98
Molecular weight: 50.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.604
IPC_protein 10.555
Toseland 10.423
ProMoST 10.262
Dawson 10.584
Bjellqvist 10.394
Wikipedia 10.847
Rodwell 10.643
Grimsley 10.672
Solomon 10.701
Lehninger 10.657
Nozaki 10.496
DTASelect 10.365
Thurlkill 10.467
EMBOSS 10.847
Sillero 10.54
Patrickios 10.233
IPC_peptide 10.701
IPC2_peptide 9.823
IPC2.peptide.svr19 8.431
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
80
0
80
39899
79
2627
498.7
53.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.316 ± 0.466
1.857 ± 0.101
5.699 ± 0.148
5.379 ± 0.191
3.406 ± 0.184
8.025 ± 0.205
2.095 ± 0.099
2.436 ± 0.133
1.556 ± 0.138
9.592 ± 0.28
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.511 ± 0.098
1.957 ± 0.158
8.346 ± 0.377
2.045 ± 0.122
9.105 ± 0.242
5.755 ± 0.192
4.827 ± 0.159
7.308 ± 0.199
1.14 ± 0.066
2.642 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here