Gloeothece verrucosa (strain PCC 7822) (Cyanothece sp. (strain PCC 7822))

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Aphanothecaceae; Gloeothece; Gloeothece verrucosa

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0U6M3|E0U6M3_GLOV7 DEAD/DEAH box helicase domain protein OS=Gloeothece verrucosa (strain PCC 7822) OX=497965 GN=Cyan7822_2822 PE=4 SV=1
MM1 pKa = 7.0ITMNATIYY9 pKa = 10.71RR10 pKa = 11.84NTNADD15 pKa = 3.32SRR17 pKa = 11.84PVSNQLIVKK26 pKa = 7.67LKK28 pKa = 10.64PEE30 pKa = 4.17VQSTQALDD38 pKa = 3.66LQKK41 pKa = 11.24SLGASVIEE49 pKa = 4.43TTKK52 pKa = 10.01QWGYY56 pKa = 8.29QLWSVTGMSIQAALAEE72 pKa = 4.5LKK74 pKa = 10.66KK75 pKa = 10.91NPNVEE80 pKa = 4.13YY81 pKa = 11.27AEE83 pKa = 4.23LNYY86 pKa = 10.76LLEE89 pKa = 4.59TSSIPNDD96 pKa = 3.23VNFNKK101 pKa = 10.14LWAINNTGQTGGTPDD116 pKa = 3.83ADD118 pKa = 3.33IDD120 pKa = 3.83GLEE123 pKa = 4.66AYY125 pKa = 10.21NLANGEE131 pKa = 4.3GVVVGVIDD139 pKa = 3.5TGVDD143 pKa = 3.4YY144 pKa = 8.2THH146 pKa = 7.75PDD148 pKa = 3.18LVNNMWTNPGEE159 pKa = 4.28VPNNGIDD166 pKa = 3.93DD167 pKa = 4.85DD168 pKa = 5.15GNGYY172 pKa = 9.53IDD174 pKa = 5.56DD175 pKa = 4.17YY176 pKa = 11.36YY177 pKa = 11.52GYY179 pKa = 10.99DD180 pKa = 3.74FVNEE184 pKa = 4.37DD185 pKa = 4.65SDD187 pKa = 4.34PLDD190 pKa = 3.72EE191 pKa = 5.22NYY193 pKa = 10.57HH194 pKa = 4.98GTHH197 pKa = 5.35VAGIIAAEE205 pKa = 4.21GNNLQGVIGVAPKK218 pKa = 9.84TKK220 pKa = 10.1IMALKK225 pKa = 9.82FLNGQEE231 pKa = 4.02TGNIFDD237 pKa = 5.84AIQAIEE243 pKa = 4.38YY244 pKa = 8.48ATMMKK249 pKa = 10.36LNYY252 pKa = 9.1GVNVQILNNSWSGGQYY268 pKa = 10.15SQALHH273 pKa = 6.69DD274 pKa = 5.79AIAAANAAGQLFTASAGNGHH294 pKa = 6.5NNNDD298 pKa = 4.22LLPVYY303 pKa = 9.13PASYY307 pKa = 10.37DD308 pKa = 3.47LEE310 pKa = 4.75NIISVAATDD319 pKa = 5.12DD320 pKa = 3.65QDD322 pKa = 3.86QLADD326 pKa = 4.2FSNYY330 pKa = 9.67GATTVDD336 pKa = 3.88LAAPGVQIYY345 pKa = 7.48STWPGNNYY353 pKa = 10.01ASSSGTSMSTAYY365 pKa = 10.55VSGVASLIYY374 pKa = 10.42SLNPNLTPAQVKK386 pKa = 10.19SYY388 pKa = 9.66ILSGVEE394 pKa = 4.23PVSSLQGITVSGGRR408 pKa = 11.84LNAYY412 pKa = 8.66KK413 pKa = 10.78ALLSPLAAKK422 pKa = 8.69ITGSLWNDD430 pKa = 3.32LDD432 pKa = 3.99QNGVRR437 pKa = 11.84DD438 pKa = 3.72AGEE441 pKa = 4.34PGLAGWKK448 pKa = 9.81IYY450 pKa = 10.79LDD452 pKa = 3.59QNNNGTFDD460 pKa = 3.7PGEE463 pKa = 4.13RR464 pKa = 11.84STVTNKK470 pKa = 10.19QGDD473 pKa = 3.95YY474 pKa = 11.33AFLLLSPGNYY484 pKa = 7.08TVRR487 pKa = 11.84EE488 pKa = 4.15VKK490 pKa = 10.36QLGWTQTNQTDD501 pKa = 4.01LYY503 pKa = 8.89YY504 pKa = 9.48TVQVAEE510 pKa = 4.31SQTVSGINWGNFLAQPATISGTKK533 pKa = 9.54WNDD536 pKa = 3.03LNQNAVLDD544 pKa = 3.97SGEE547 pKa = 4.43PGLAGWTIYY556 pKa = 10.84LDD558 pKa = 3.78EE559 pKa = 5.2NGNSQLDD566 pKa = 3.65TGEE569 pKa = 4.53LSTVTDD575 pKa = 3.63EE576 pKa = 3.86QGKK579 pKa = 10.38YY580 pKa = 10.68SFTNLAPGNYY590 pKa = 8.41QVNVVNQNGWQQTQPVSGSYY610 pKa = 10.35SVALSPDD617 pKa = 3.11EE618 pKa = 3.99VVNNINFGHH627 pKa = 6.04YY628 pKa = 7.77EE629 pKa = 3.99LKK631 pKa = 10.49KK632 pKa = 10.98GEE634 pKa = 3.75IRR636 pKa = 11.84GIKK639 pKa = 9.3WNDD642 pKa = 3.16LDD644 pKa = 4.6GDD646 pKa = 4.48GIRR649 pKa = 11.84DD650 pKa = 3.46QGEE653 pKa = 3.95PGLPGMTIYY662 pKa = 11.02LDD664 pKa = 3.47QNQNGRR670 pKa = 11.84LDD672 pKa = 3.6NGEE675 pKa = 3.9ISALTDD681 pKa = 3.12KK682 pKa = 11.04DD683 pKa = 3.41GNYY686 pKa = 10.8AFTNLTPGITYY697 pKa = 8.57TVAEE701 pKa = 4.25VLQANWAQTTPRR713 pKa = 11.84FLFNADD719 pKa = 3.18FSDD722 pKa = 3.86AQGNASLDD730 pKa = 3.37GFTIDD735 pKa = 4.58NNYY738 pKa = 9.86NGLWHH743 pKa = 7.22LSTGRR748 pKa = 11.84GNQAGHH754 pKa = 6.19SADD757 pKa = 3.46DD758 pKa = 3.5SLYY761 pKa = 10.25FGTKK765 pKa = 9.69EE766 pKa = 4.35GIDD769 pKa = 3.29GGGNYY774 pKa = 10.19DD775 pKa = 3.42VGSTSGRR782 pKa = 11.84ITSSEE787 pKa = 3.4ISLIGLEE794 pKa = 4.18DD795 pKa = 3.58AQLSFNYY802 pKa = 9.8FLQTEE807 pKa = 4.24GGGFYY812 pKa = 10.83DD813 pKa = 3.38QAKK816 pKa = 10.37VLLSQNGGDD825 pKa = 3.97FFQIASNQQSLQDD838 pKa = 4.06PTPGWTNLTLDD849 pKa = 4.0LTAYY853 pKa = 8.37TGSKK857 pKa = 9.6IKK859 pKa = 10.31IRR861 pKa = 11.84FDD863 pKa = 3.54FDD865 pKa = 4.73SIDD868 pKa = 3.54YY869 pKa = 9.41YY870 pKa = 11.89ANNFEE875 pKa = 4.08GWYY878 pKa = 9.64IDD880 pKa = 5.73DD881 pKa = 3.58ITVTNAAGQGIYY893 pKa = 7.63TVKK896 pKa = 10.47LDD898 pKa = 3.77PGQIVNNINFGNYY911 pKa = 7.61QPVKK915 pKa = 10.41NGTWRR920 pKa = 11.84NDD922 pKa = 3.22EE923 pKa = 4.92LIGTKK928 pKa = 10.35LKK930 pKa = 10.83DD931 pKa = 3.86IINGLAGNDD940 pKa = 3.34NLYY943 pKa = 10.97GKK945 pKa = 9.97EE946 pKa = 4.33DD947 pKa = 3.85NDD949 pKa = 3.66VLNGGGGNDD958 pKa = 3.04NLYY961 pKa = 11.1GNEE964 pKa = 4.59GNDD967 pKa = 3.28EE968 pKa = 4.41LNGNEE973 pKa = 4.06GKK975 pKa = 10.26DD976 pKa = 3.67YY977 pKa = 11.73LEE979 pKa = 4.73GNQGNDD985 pKa = 3.09SLNGGSEE992 pKa = 4.05SDD994 pKa = 3.36NLLGNEE1000 pKa = 4.79GNDD1003 pKa = 3.54TLKK1006 pKa = 11.14GGTHH1010 pKa = 7.18NDD1012 pKa = 4.06TLWGNQGNDD1021 pKa = 3.15ILNGDD1026 pKa = 4.19EE1027 pKa = 5.68GNDD1030 pKa = 3.46LLYY1033 pKa = 11.16GNEE1036 pKa = 4.22GQDD1039 pKa = 3.53TLKK1042 pKa = 11.05GGSNNDD1048 pKa = 3.28TLYY1051 pKa = 11.37GNEE1054 pKa = 4.66DD1055 pKa = 3.53NDD1057 pKa = 4.29LLSGDD1062 pKa = 4.01EE1063 pKa = 5.38SNDD1066 pKa = 3.28QLYY1069 pKa = 10.87GDD1071 pKa = 4.3QGNDD1075 pKa = 3.11ILNGDD1080 pKa = 4.3AGNDD1084 pKa = 3.38ALYY1087 pKa = 10.82GSTGNDD1093 pKa = 3.24TLSGGVGRR1101 pKa = 11.84DD1102 pKa = 3.17TLYY1105 pKa = 11.43GNEE1108 pKa = 4.53NNDD1111 pKa = 4.55LLNGDD1116 pKa = 4.88DD1117 pKa = 5.65DD1118 pKa = 5.76HH1119 pKa = 7.97DD1120 pKa = 4.3LLYY1123 pKa = 11.21GNQDD1127 pKa = 2.96NDD1129 pKa = 3.87TLNGGNGNDD1138 pKa = 3.27TLYY1141 pKa = 11.19GNEE1144 pKa = 4.67GSDD1147 pKa = 4.2ILNGGNNNDD1156 pKa = 3.34SLYY1159 pKa = 11.11GNEE1162 pKa = 5.46GNDD1165 pKa = 3.68TLNGAEE1171 pKa = 4.58GNDD1174 pKa = 3.62TLRR1177 pKa = 11.84GGEE1180 pKa = 4.3GNDD1183 pKa = 3.86LLTGGLDD1190 pKa = 3.08KK1191 pKa = 11.34DD1192 pKa = 4.35VFVLAANFGSDD1203 pKa = 3.66TITDD1207 pKa = 4.05FMDD1210 pKa = 4.25GQDD1213 pKa = 4.22KK1214 pKa = 11.18LGLSAGLSFAQLTLTSNQSNTLIYY1238 pKa = 9.26LTSSNEE1244 pKa = 3.88LLATLSGINLSLINSRR1260 pKa = 11.84DD1261 pKa = 3.4FMVII1265 pKa = 3.31

Molecular weight: 135.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E0U8M4|E0U8M4_GLOV7 Serine/threonine protein kinase OS=Gloeothece verrucosa (strain PCC 7822) OX=497965 GN=Cyan7822_1783 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84RR3 pKa = 11.84LLCYY7 pKa = 10.09QEE9 pKa = 5.72LVFHH13 pKa = 5.43QQHH16 pKa = 6.48YY17 pKa = 10.81IDD19 pKa = 5.25LLNSGQVDD27 pKa = 4.11PLAHH31 pKa = 6.68SLIRR35 pKa = 11.84WHH37 pKa = 6.0IRR39 pKa = 11.84KK40 pKa = 9.48LGRR43 pKa = 11.84EE44 pKa = 3.85IDD46 pKa = 3.98TRR48 pKa = 11.84WPPRR52 pKa = 11.84LRR54 pKa = 11.84KK55 pKa = 9.64VHH57 pKa = 5.09VV58 pKa = 3.53

Molecular weight: 7.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6559

0

6559

2140918

30

11342

326.4

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.295 ± 0.033

0.98 ± 0.012

4.826 ± 0.033

6.522 ± 0.042

4.062 ± 0.023

6.458 ± 0.041

1.758 ± 0.018

7.158 ± 0.026

5.504 ± 0.037

11.158 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.703 ± 0.018

4.829 ± 0.043

4.716 ± 0.025

5.404 ± 0.032

4.608 ± 0.033

6.549 ± 0.03

5.641 ± 0.049

6.036 ± 0.026

1.459 ± 0.015

3.333 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski